Neonatally induced mild diabetes: influence on development, behavior and reproductive function of female Wistar rats
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/1758-5996-5-61 http://hdl.handle.net/11449/112622 |
Resumo: | Background: Neonatal STZ treatment induces a state of mild hyperglycemia in adult rats that disrupts metabolism and maternal/fetal interactions. The aim of this study was investigate the effect of neonatal STZ treatment on the physical development, behavior, and reproductive function of female Wistar rats from infancy to adulthood.Methods: At birth, litters were assigned either to a Control (subcutaneous (s.c.) citrate buffer, n = 10) or STZ group, (streptozotocin (STZ) - 100 mg/kg-sc, n = 6). Blood glucose levels were measured on postnatal days (PND) 35, 84 and 120. In Experiment 1 body weight, length and the appearance of developmental milestones such as eye and vaginal opening were monitored. To assess the relative contribution of the initial and long term effects of STZ treatment this group was subdivided based on blood glucose levels recorded on PND 120: STZ hyperglycemic (between 120 and 300 mg/dl) and STZ normoglycemic (under 120 mg/dl). Behavioral activity was assessed in an open field on PND 21 and 75. In Experiment 2 estrous cyclicity, sexual behavior and circulating gonadotropin, ovarian steroid, and insulin levels were compared between control and STZ-hyperglycemic rats. In all measures the litter was the experimental unit. Parametric data were analyzed using one-way or, where appropriate, two-way ANOVA and significant effects were investigated using Tukey's post hoc test. Fisher's exact test was employed when data did not satisfy the assumption of normality e. g. presence of urine and fecal boli on the open field between groups. Statistical significance was set at p < 0.05 for all data.Results: As expected neonatal STZ treatment caused hyperglycemia and hypoinsulinemia in adulthood. STZ-treated pups also showed a temporary reduction in growth rate that probably reflected the early loss of circulating insulin. Hyperglycemic rats also exhibited a reduction in locomotor and exploratory behavior in the open field. Mild hyperglycemia did not impair gonadotropin levels or estrous cylicity but ovarian steroid concentrations were altered.Conclusions: In female Wistar rats, neonatal STZ treatment impairs growth in infancy and results in mild hyperglycemia/hypoinsulinemia in adulthood that is associated with changes in the response to a novel environment and altered ovarian steroid hormone levels. |
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Repositório Institucional da UNESP |
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Neonatally induced mild diabetes: influence on development, behavior and reproductive function of female Wistar ratsMild diabetesStreptozotocinRatDevelopmentBehaviorReproductive functionBackground: Neonatal STZ treatment induces a state of mild hyperglycemia in adult rats that disrupts metabolism and maternal/fetal interactions. The aim of this study was investigate the effect of neonatal STZ treatment on the physical development, behavior, and reproductive function of female Wistar rats from infancy to adulthood.Methods: At birth, litters were assigned either to a Control (subcutaneous (s.c.) citrate buffer, n = 10) or STZ group, (streptozotocin (STZ) - 100 mg/kg-sc, n = 6). Blood glucose levels were measured on postnatal days (PND) 35, 84 and 120. In Experiment 1 body weight, length and the appearance of developmental milestones such as eye and vaginal opening were monitored. To assess the relative contribution of the initial and long term effects of STZ treatment this group was subdivided based on blood glucose levels recorded on PND 120: STZ hyperglycemic (between 120 and 300 mg/dl) and STZ normoglycemic (under 120 mg/dl). Behavioral activity was assessed in an open field on PND 21 and 75. In Experiment 2 estrous cyclicity, sexual behavior and circulating gonadotropin, ovarian steroid, and insulin levels were compared between control and STZ-hyperglycemic rats. In all measures the litter was the experimental unit. Parametric data were analyzed using one-way or, where appropriate, two-way ANOVA and significant effects were investigated using Tukey's post hoc test. Fisher's exact test was employed when data did not satisfy the assumption of normality e. g. presence of urine and fecal boli on the open field between groups. Statistical significance was set at p < 0.05 for all data.Results: As expected neonatal STZ treatment caused hyperglycemia and hypoinsulinemia in adulthood. STZ-treated pups also showed a temporary reduction in growth rate that probably reflected the early loss of circulating insulin. Hyperglycemic rats also exhibited a reduction in locomotor and exploratory behavior in the open field. Mild hyperglycemia did not impair gonadotropin levels or estrous cylicity but ovarian steroid concentrations were altered.Conclusions: In female Wistar rats, neonatal STZ treatment impairs growth in infancy and results in mild hyperglycemia/hypoinsulinemia in adulthood that is associated with changes in the response to a novel environment and altered ovarian steroid hormone levels.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Sao Paulo State Univ, Botucatu Biosci Inst, Dept Physiol, BR-18618970 Sao Paulo, BrazilSao Paulo State Univ Unesp, Botucatu Med Sch, Dept Gynecol & Obstet, Lab Expt Res Gynecol & Obstet, BR-18618970 Sao Paulo, BrazilConcordia Univ, Dept Psychol, Ctr Studies Behav Neurobiol, Montreal, PQ H4B 1R6, CanadaFed Univ ABC, Math Computat & Cognit Ctr, BR-09210580 Sao Paulo, BrazilUniv Paulista, Grad Program Environm & Expt Pathol, Sao Paulo, BrazilUniv Paulista, Grad Program Dent, Sao Paulo, BrazilSao Paulo State Univ, Botucatu Biosci Inst, Dept Morphol, BR-18618970 Sao Paulo, BrazilUniv Sao Paulo, Fac Odontol Ribeirao Preto, Dept Morfol Estomatol & Fisiol, Lab Neuroendocrinol Repdroducao, BR-14040904 Sao Paulo, BrazilSao Paulo State Univ, Botucatu Biosci Inst, Dept Physiol, BR-18618970 Sao Paulo, BrazilSao Paulo State Univ Unesp, Botucatu Med Sch, Dept Gynecol & Obstet, Lab Expt Res Gynecol & Obstet, BR-18618970 Sao Paulo, BrazilSao Paulo State Univ, Botucatu Biosci Inst, Dept Morphol, BR-18618970 Sao Paulo, BrazilBiomed Central Ltd.Universidade Estadual Paulista (Unesp)Concordia UnivUniversidade Federal do ABC (UFABC)Univ PaulistaUniversidade de São Paulo (USP)Inhasz Kiss, Ana Carolina [UNESP]Woodside, BarbaraSinzato, Yuri Karen [UNESP]Bernardi, Maria MarthaKempinas, Wilma De Grava [UNESP]Anselmo-Franci, Janete AparecidaDamasceno, Debora Cristina [UNESP]2014-12-03T13:10:53Z2014-12-03T13:10:53Z2013-10-16info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10application/pdfhttp://dx.doi.org/10.1186/1758-5996-5-61Diabetology & Metabolic Syndrome. London: Biomed Central Ltd, v. 5, 10 p., 2013.1758-5996http://hdl.handle.net/11449/11262210.1186/1758-5996-5-61WOS:000326633000001WOS000326633000001.pdf6326450271169741Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDiabetology & Metabolic Syndrome2.4130,943info:eu-repo/semantics/openAccess2024-08-16T14:12:50Zoai:repositorio.unesp.br:11449/112622Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-16T14:12:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Neonatally induced mild diabetes: influence on development, behavior and reproductive function of female Wistar rats |
title |
Neonatally induced mild diabetes: influence on development, behavior and reproductive function of female Wistar rats |
spellingShingle |
Neonatally induced mild diabetes: influence on development, behavior and reproductive function of female Wistar rats Inhasz Kiss, Ana Carolina [UNESP] Mild diabetes Streptozotocin Rat Development Behavior Reproductive function |
title_short |
Neonatally induced mild diabetes: influence on development, behavior and reproductive function of female Wistar rats |
title_full |
Neonatally induced mild diabetes: influence on development, behavior and reproductive function of female Wistar rats |
title_fullStr |
Neonatally induced mild diabetes: influence on development, behavior and reproductive function of female Wistar rats |
title_full_unstemmed |
Neonatally induced mild diabetes: influence on development, behavior and reproductive function of female Wistar rats |
title_sort |
Neonatally induced mild diabetes: influence on development, behavior and reproductive function of female Wistar rats |
author |
Inhasz Kiss, Ana Carolina [UNESP] |
author_facet |
Inhasz Kiss, Ana Carolina [UNESP] Woodside, Barbara Sinzato, Yuri Karen [UNESP] Bernardi, Maria Martha Kempinas, Wilma De Grava [UNESP] Anselmo-Franci, Janete Aparecida Damasceno, Debora Cristina [UNESP] |
author_role |
author |
author2 |
Woodside, Barbara Sinzato, Yuri Karen [UNESP] Bernardi, Maria Martha Kempinas, Wilma De Grava [UNESP] Anselmo-Franci, Janete Aparecida Damasceno, Debora Cristina [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Concordia Univ Universidade Federal do ABC (UFABC) Univ Paulista Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Inhasz Kiss, Ana Carolina [UNESP] Woodside, Barbara Sinzato, Yuri Karen [UNESP] Bernardi, Maria Martha Kempinas, Wilma De Grava [UNESP] Anselmo-Franci, Janete Aparecida Damasceno, Debora Cristina [UNESP] |
dc.subject.por.fl_str_mv |
Mild diabetes Streptozotocin Rat Development Behavior Reproductive function |
topic |
Mild diabetes Streptozotocin Rat Development Behavior Reproductive function |
description |
Background: Neonatal STZ treatment induces a state of mild hyperglycemia in adult rats that disrupts metabolism and maternal/fetal interactions. The aim of this study was investigate the effect of neonatal STZ treatment on the physical development, behavior, and reproductive function of female Wistar rats from infancy to adulthood.Methods: At birth, litters were assigned either to a Control (subcutaneous (s.c.) citrate buffer, n = 10) or STZ group, (streptozotocin (STZ) - 100 mg/kg-sc, n = 6). Blood glucose levels were measured on postnatal days (PND) 35, 84 and 120. In Experiment 1 body weight, length and the appearance of developmental milestones such as eye and vaginal opening were monitored. To assess the relative contribution of the initial and long term effects of STZ treatment this group was subdivided based on blood glucose levels recorded on PND 120: STZ hyperglycemic (between 120 and 300 mg/dl) and STZ normoglycemic (under 120 mg/dl). Behavioral activity was assessed in an open field on PND 21 and 75. In Experiment 2 estrous cyclicity, sexual behavior and circulating gonadotropin, ovarian steroid, and insulin levels were compared between control and STZ-hyperglycemic rats. In all measures the litter was the experimental unit. Parametric data were analyzed using one-way or, where appropriate, two-way ANOVA and significant effects were investigated using Tukey's post hoc test. Fisher's exact test was employed when data did not satisfy the assumption of normality e. g. presence of urine and fecal boli on the open field between groups. Statistical significance was set at p < 0.05 for all data.Results: As expected neonatal STZ treatment caused hyperglycemia and hypoinsulinemia in adulthood. STZ-treated pups also showed a temporary reduction in growth rate that probably reflected the early loss of circulating insulin. Hyperglycemic rats also exhibited a reduction in locomotor and exploratory behavior in the open field. Mild hyperglycemia did not impair gonadotropin levels or estrous cylicity but ovarian steroid concentrations were altered.Conclusions: In female Wistar rats, neonatal STZ treatment impairs growth in infancy and results in mild hyperglycemia/hypoinsulinemia in adulthood that is associated with changes in the response to a novel environment and altered ovarian steroid hormone levels. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-10-16 2014-12-03T13:10:53Z 2014-12-03T13:10:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/1758-5996-5-61 Diabetology & Metabolic Syndrome. London: Biomed Central Ltd, v. 5, 10 p., 2013. 1758-5996 http://hdl.handle.net/11449/112622 10.1186/1758-5996-5-61 WOS:000326633000001 WOS000326633000001.pdf 6326450271169741 |
url |
http://dx.doi.org/10.1186/1758-5996-5-61 http://hdl.handle.net/11449/112622 |
identifier_str_mv |
Diabetology & Metabolic Syndrome. London: Biomed Central Ltd, v. 5, 10 p., 2013. 1758-5996 10.1186/1758-5996-5-61 WOS:000326633000001 WOS000326633000001.pdf 6326450271169741 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Diabetology & Metabolic Syndrome 2.413 0,943 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
10 application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd. |
publisher.none.fl_str_mv |
Biomed Central Ltd. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128194243461120 |