Comparison of Two Methodologies for CD4+T Lymphocytes Relative Counting on Immune Monitoring of Patients with Human Immunodeficiency Virus
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1100/2012/906873 http://hdl.handle.net/11449/7066 |
Resumo: | Considering that counting the percentage of CD4 T lymphocytes can add prognostic information regarding patients infected with HIV, the aim of this study was to evaluate the percentage values of CD4+ T lymphocytes from 81 patients determined by flow cytometry and estimated by flow cytometry in conjunction with a hematology counter. Means were compared through the Student's t-test. Pearson's correlation was determined, and the agreement between results was tested by Bland-Altman. The level of significance was P < 0.05. It was found a significantly higher mean difference between the relative values of CD4+ T lymphocytes to the hematologic counter (P < 0.05), for all strata studied. Positive and significant correlations (P < 0.01) were found between the strata CD4 < 200 cells/mL (r = 0.93), between 200 and 500 cells/mL (r = 0.65), and >500 cells/mL (r = 0.81). The limits of agreement were 1.0 +/- 3.8% for the stratum of CD4 < 200 cells/mL, approximately 2.2 +/- 13.5% for the stratum of CD4 between 200 and 500 cells/mL, and approximately 6.2 +/- 20.4% for the stratum > 500 cells/mL. The differences in the percentages of CD4+ T lymphocytes obtained by different methodologies could lead to conflict when used in clinical decisions related to the treatment and care of people infected with HIV. |
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Repositório Institucional da UNESP |
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spelling |
Comparison of Two Methodologies for CD4+T Lymphocytes Relative Counting on Immune Monitoring of Patients with Human Immunodeficiency VirusConsidering that counting the percentage of CD4 T lymphocytes can add prognostic information regarding patients infected with HIV, the aim of this study was to evaluate the percentage values of CD4+ T lymphocytes from 81 patients determined by flow cytometry and estimated by flow cytometry in conjunction with a hematology counter. Means were compared through the Student's t-test. Pearson's correlation was determined, and the agreement between results was tested by Bland-Altman. The level of significance was P < 0.05. It was found a significantly higher mean difference between the relative values of CD4+ T lymphocytes to the hematologic counter (P < 0.05), for all strata studied. Positive and significant correlations (P < 0.01) were found between the strata CD4 < 200 cells/mL (r = 0.93), between 200 and 500 cells/mL (r = 0.65), and >500 cells/mL (r = 0.81). The limits of agreement were 1.0 +/- 3.8% for the stratum of CD4 < 200 cells/mL, approximately 2.2 +/- 13.5% for the stratum of CD4 between 200 and 500 cells/mL, and approximately 6.2 +/- 20.4% for the stratum > 500 cells/mL. The differences in the percentages of CD4+ T lymphocytes obtained by different methodologies could lead to conflict when used in clinical decisions related to the treatment and care of people infected with HIV.State Univ Ponta Grossa UEPG, Pharmaceut Sci Post Grad Program, BR-84030900 Ponta Grossa, PR, BrazilState Univ Parana Midwestern UNICTR, Dept Pharm, BR-85040080 Guarapuava, PR, BrazilEAS, Cty Hlth Dept, BR-84051900 Ponta Grossa, PR, BrazilState Univ Paulista UNESP, Dept Physiotherapy, BR-19060900 Presidente Prudente, SP, BrazilState Univ Paulista UNESP, Dept Physiotherapy, BR-19060900 Presidente Prudente, SP, BrazilHindawi Publishing CorporationUniversidade Estadual de Ponta Grossa (UEPG)State Univ Parana Midwestern UNICTREASUniversidade Estadual Paulista (Unesp)Kalva Borato, Danielle CristyaneCarraro, EmersonWeber Ribas, Sonia ReginaKalva-Filho, Carlos Augusto [UNESP]Rebuglio Vellosa, Jose Carlos2014-05-20T13:23:26Z2014-05-20T13:23:26Z2012-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article5application/pdfhttp://dx.doi.org/10.1100/2012/906873Scientific World Journal. New York: Hindawi Publishing Corporation, p. 5, 2012.1537-744Xhttp://hdl.handle.net/11449/706610.1100/2012/906873WOS:000312025000001WOS000312025000001.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific World Journalinfo:eu-repo/semantics/openAccess2024-06-18T18:44:28Zoai:repositorio.unesp.br:11449/7066Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:55:33.669172Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Comparison of Two Methodologies for CD4+T Lymphocytes Relative Counting on Immune Monitoring of Patients with Human Immunodeficiency Virus |
title |
Comparison of Two Methodologies for CD4+T Lymphocytes Relative Counting on Immune Monitoring of Patients with Human Immunodeficiency Virus |
spellingShingle |
Comparison of Two Methodologies for CD4+T Lymphocytes Relative Counting on Immune Monitoring of Patients with Human Immunodeficiency Virus Kalva Borato, Danielle Cristyane |
title_short |
Comparison of Two Methodologies for CD4+T Lymphocytes Relative Counting on Immune Monitoring of Patients with Human Immunodeficiency Virus |
title_full |
Comparison of Two Methodologies for CD4+T Lymphocytes Relative Counting on Immune Monitoring of Patients with Human Immunodeficiency Virus |
title_fullStr |
Comparison of Two Methodologies for CD4+T Lymphocytes Relative Counting on Immune Monitoring of Patients with Human Immunodeficiency Virus |
title_full_unstemmed |
Comparison of Two Methodologies for CD4+T Lymphocytes Relative Counting on Immune Monitoring of Patients with Human Immunodeficiency Virus |
title_sort |
Comparison of Two Methodologies for CD4+T Lymphocytes Relative Counting on Immune Monitoring of Patients with Human Immunodeficiency Virus |
author |
Kalva Borato, Danielle Cristyane |
author_facet |
Kalva Borato, Danielle Cristyane Carraro, Emerson Weber Ribas, Sonia Regina Kalva-Filho, Carlos Augusto [UNESP] Rebuglio Vellosa, Jose Carlos |
author_role |
author |
author2 |
Carraro, Emerson Weber Ribas, Sonia Regina Kalva-Filho, Carlos Augusto [UNESP] Rebuglio Vellosa, Jose Carlos |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Ponta Grossa (UEPG) State Univ Parana Midwestern UNICTR EAS Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Kalva Borato, Danielle Cristyane Carraro, Emerson Weber Ribas, Sonia Regina Kalva-Filho, Carlos Augusto [UNESP] Rebuglio Vellosa, Jose Carlos |
description |
Considering that counting the percentage of CD4 T lymphocytes can add prognostic information regarding patients infected with HIV, the aim of this study was to evaluate the percentage values of CD4+ T lymphocytes from 81 patients determined by flow cytometry and estimated by flow cytometry in conjunction with a hematology counter. Means were compared through the Student's t-test. Pearson's correlation was determined, and the agreement between results was tested by Bland-Altman. The level of significance was P < 0.05. It was found a significantly higher mean difference between the relative values of CD4+ T lymphocytes to the hematologic counter (P < 0.05), for all strata studied. Positive and significant correlations (P < 0.01) were found between the strata CD4 < 200 cells/mL (r = 0.93), between 200 and 500 cells/mL (r = 0.65), and >500 cells/mL (r = 0.81). The limits of agreement were 1.0 +/- 3.8% for the stratum of CD4 < 200 cells/mL, approximately 2.2 +/- 13.5% for the stratum of CD4 between 200 and 500 cells/mL, and approximately 6.2 +/- 20.4% for the stratum > 500 cells/mL. The differences in the percentages of CD4+ T lymphocytes obtained by different methodologies could lead to conflict when used in clinical decisions related to the treatment and care of people infected with HIV. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01 2014-05-20T13:23:26Z 2014-05-20T13:23:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1100/2012/906873 Scientific World Journal. New York: Hindawi Publishing Corporation, p. 5, 2012. 1537-744X http://hdl.handle.net/11449/7066 10.1100/2012/906873 WOS:000312025000001 WOS000312025000001.pdf |
url |
http://dx.doi.org/10.1100/2012/906873 http://hdl.handle.net/11449/7066 |
identifier_str_mv |
Scientific World Journal. New York: Hindawi Publishing Corporation, p. 5, 2012. 1537-744X 10.1100/2012/906873 WOS:000312025000001 WOS000312025000001.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific World Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
5 application/pdf |
dc.publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129373749903360 |