Evaluation of 4% stabilized Sodium Hypochlorite activity in the repair of cutaneous excisional wounds in mice

Detalhes bibliográficos
Autor(a) principal: da Costa, Marcelo C. [UNESP]
Data de Publicação: 2021
Outros Autores: Ferreira, Bruno A., de Moura, Francyelle B.R., de Lima, Lara G., Araujo, Fernanda de A., Mota, Francisco C.D.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.injury.2021.05.035
http://hdl.handle.net/11449/221828
Resumo: Wounds are conditions largely present in the clinical routine, and even though frequent, their complete resolution can be challenging. Several solutions can aid or stimulate the healing process, and for this reason, this work used a stabilized solution of 4% sodium hypochlorite for the treatment of excisional wounds in mice. This study was carried out in two distinct stages: in the first stage, the optimal concentration of the chlorinated solution was determined by using the sponge implantation technique in mouse subcutaneous tissue to evaluate the dose-response curve; and in the second phase, this concentration was tested in an experimental model of excisional skin wounds in mice. Soluble collagen, hemoglobin, myeloperoxidase (MPO) and N-acetyl-β-D-glycosaminidase (NAG) activity were assessed, and total, type I and type III collagen deposition were quantified in both stages. Based on the results presented in the sponge implantation study, the chlorinated solution at 150 ppm (0.015%) was chosen for use in a preclinical trial of skin healing in mice. At 1, 3, 7 and 14 days of treatment, the % wound area repair in the group treated with 150 ppm chlorinated solution was higher when compared to the control group, with statistical differences at all time points (*p≤ 0.05 and **p≤ 0.01). 150 ppm chlorinated solution obtained from a stabilized 4% sodium hypochlorite solution was effective in accelerating cutaneous excision wound repair in mice, showing a positive influence on tissue repair.
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spelling Evaluation of 4% stabilized Sodium Hypochlorite activity in the repair of cutaneous excisional wounds in miceChlorinated speciesCollagenEnzymesInflammationMacrophagesMyeloperoxidaseNeutrophilsSpongeWounds are conditions largely present in the clinical routine, and even though frequent, their complete resolution can be challenging. Several solutions can aid or stimulate the healing process, and for this reason, this work used a stabilized solution of 4% sodium hypochlorite for the treatment of excisional wounds in mice. This study was carried out in two distinct stages: in the first stage, the optimal concentration of the chlorinated solution was determined by using the sponge implantation technique in mouse subcutaneous tissue to evaluate the dose-response curve; and in the second phase, this concentration was tested in an experimental model of excisional skin wounds in mice. Soluble collagen, hemoglobin, myeloperoxidase (MPO) and N-acetyl-β-D-glycosaminidase (NAG) activity were assessed, and total, type I and type III collagen deposition were quantified in both stages. Based on the results presented in the sponge implantation study, the chlorinated solution at 150 ppm (0.015%) was chosen for use in a preclinical trial of skin healing in mice. At 1, 3, 7 and 14 days of treatment, the % wound area repair in the group treated with 150 ppm chlorinated solution was higher when compared to the control group, with statistical differences at all time points (*p≤ 0.05 and **p≤ 0.01). 150 ppm chlorinated solution obtained from a stabilized 4% sodium hypochlorite solution was effective in accelerating cutaneous excision wound repair in mice, showing a positive influence on tissue repair.Department of Veterinary Surgery Paulista State University Júlio de Mesquita Filho Access Road Highway Professor Paulo Donato Castelane Castelane S / N Vila IndustrialInstitute of Biomedical Sciences Federal University of Uberlândia Avenue. João Naves de Ávila, 2121 - Santa Monica, [Zip Code]: 1720Institute of Biology Campinas State University, Street Monteiro Lobato, 255. [Zip Code]: 1308-862Veterinarian Federal University of Uberlândia, Avenue Mato Grosso, 3289 – Código postal [Zip Code]: 38405-314, Bloco 2S - UmuaramaDepartment of Veterinary Surgery Federal University of Uberlândia, Av. Mato Grosso, 3289 - [Zip Code]: 38405-314, Bloco 2S - UmuaramaDepartment of Veterinary Surgery Paulista State University Júlio de Mesquita Filho Access Road Highway Professor Paulo Donato Castelane Castelane S / N Vila IndustrialUniversidade Estadual Paulista (UNESP)Universidade Federal de Uberlândia (UFU)Universidade Estadual de Campinas (UNICAMP)da Costa, Marcelo C. [UNESP]Ferreira, Bruno A.de Moura, Francyelle B.R.de Lima, Lara G.Araujo, Fernanda de A.Mota, Francisco C.D.2022-04-28T19:40:51Z2022-04-28T19:40:51Z2021-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2075-2083http://dx.doi.org/10.1016/j.injury.2021.05.035Injury, v. 52, n. 8, p. 2075-2083, 2021.1879-02670020-1383http://hdl.handle.net/11449/22182810.1016/j.injury.2021.05.0352-s2.0-85108535509Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInjuryinfo:eu-repo/semantics/openAccess2022-04-28T19:40:51Zoai:repositorio.unesp.br:11449/221828Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:00:57.484303Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Evaluation of 4% stabilized Sodium Hypochlorite activity in the repair of cutaneous excisional wounds in mice
title Evaluation of 4% stabilized Sodium Hypochlorite activity in the repair of cutaneous excisional wounds in mice
spellingShingle Evaluation of 4% stabilized Sodium Hypochlorite activity in the repair of cutaneous excisional wounds in mice
da Costa, Marcelo C. [UNESP]
Chlorinated species
Collagen
Enzymes
Inflammation
Macrophages
Myeloperoxidase
Neutrophils
Sponge
title_short Evaluation of 4% stabilized Sodium Hypochlorite activity in the repair of cutaneous excisional wounds in mice
title_full Evaluation of 4% stabilized Sodium Hypochlorite activity in the repair of cutaneous excisional wounds in mice
title_fullStr Evaluation of 4% stabilized Sodium Hypochlorite activity in the repair of cutaneous excisional wounds in mice
title_full_unstemmed Evaluation of 4% stabilized Sodium Hypochlorite activity in the repair of cutaneous excisional wounds in mice
title_sort Evaluation of 4% stabilized Sodium Hypochlorite activity in the repair of cutaneous excisional wounds in mice
author da Costa, Marcelo C. [UNESP]
author_facet da Costa, Marcelo C. [UNESP]
Ferreira, Bruno A.
de Moura, Francyelle B.R.
de Lima, Lara G.
Araujo, Fernanda de A.
Mota, Francisco C.D.
author_role author
author2 Ferreira, Bruno A.
de Moura, Francyelle B.R.
de Lima, Lara G.
Araujo, Fernanda de A.
Mota, Francisco C.D.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade Federal de Uberlândia (UFU)
Universidade Estadual de Campinas (UNICAMP)
dc.contributor.author.fl_str_mv da Costa, Marcelo C. [UNESP]
Ferreira, Bruno A.
de Moura, Francyelle B.R.
de Lima, Lara G.
Araujo, Fernanda de A.
Mota, Francisco C.D.
dc.subject.por.fl_str_mv Chlorinated species
Collagen
Enzymes
Inflammation
Macrophages
Myeloperoxidase
Neutrophils
Sponge
topic Chlorinated species
Collagen
Enzymes
Inflammation
Macrophages
Myeloperoxidase
Neutrophils
Sponge
description Wounds are conditions largely present in the clinical routine, and even though frequent, their complete resolution can be challenging. Several solutions can aid or stimulate the healing process, and for this reason, this work used a stabilized solution of 4% sodium hypochlorite for the treatment of excisional wounds in mice. This study was carried out in two distinct stages: in the first stage, the optimal concentration of the chlorinated solution was determined by using the sponge implantation technique in mouse subcutaneous tissue to evaluate the dose-response curve; and in the second phase, this concentration was tested in an experimental model of excisional skin wounds in mice. Soluble collagen, hemoglobin, myeloperoxidase (MPO) and N-acetyl-β-D-glycosaminidase (NAG) activity were assessed, and total, type I and type III collagen deposition were quantified in both stages. Based on the results presented in the sponge implantation study, the chlorinated solution at 150 ppm (0.015%) was chosen for use in a preclinical trial of skin healing in mice. At 1, 3, 7 and 14 days of treatment, the % wound area repair in the group treated with 150 ppm chlorinated solution was higher when compared to the control group, with statistical differences at all time points (*p≤ 0.05 and **p≤ 0.01). 150 ppm chlorinated solution obtained from a stabilized 4% sodium hypochlorite solution was effective in accelerating cutaneous excision wound repair in mice, showing a positive influence on tissue repair.
publishDate 2021
dc.date.none.fl_str_mv 2021-08-01
2022-04-28T19:40:51Z
2022-04-28T19:40:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.injury.2021.05.035
Injury, v. 52, n. 8, p. 2075-2083, 2021.
1879-0267
0020-1383
http://hdl.handle.net/11449/221828
10.1016/j.injury.2021.05.035
2-s2.0-85108535509
url http://dx.doi.org/10.1016/j.injury.2021.05.035
http://hdl.handle.net/11449/221828
identifier_str_mv Injury, v. 52, n. 8, p. 2075-2083, 2021.
1879-0267
0020-1383
10.1016/j.injury.2021.05.035
2-s2.0-85108535509
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Injury
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 2075-2083
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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