High final energy of gallium arsenide laser increases MyoD gene expression during the intermediate phase of muscle regeneration after cryoinjury in rats
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10103-018-2439-3 http://hdl.handle.net/11449/170603 |
Resumo: | The aim of this study was to determine the effects of gallium arsenide (GaAs) laser on IGF-I, MyoD, MAFbx, and TNF-α gene expression during the intermediate phase of muscle regeneration after cryoinjury 21 Wistar rats were divided into three groups (n = 7 per group): untreated with no injury (control group), cryoinjury without GaAs (injured group), and cryoinjury with GaAs (GaAs-injured group). The cryoinjury was induced in the central region of the tibialis anterior muscle (TA). The region injured was irradiated once a day during 14 days using GaAs laser (904 nm; spot size 0.035 cm2, output power 50 mW; energy density 69 J cm−2; exposure time 4 s per point; final energy 4.8 J). Twenty-four hours after the last application, the right and left TA muscles were collected for histological (collagen content) and molecular (gene expression of IGF-I, MyoD, MAFbx, and TNF-α) analyses, respectively. Data were analyzed using one-way ANOVA at P < 0.05. There were no significant (P > 0.05) differences in collagen density and IGF-I gene expression in all experimental groups. There were similar (P < 0.05) decreases in MAFbx and TNF-α gene expression in the injured and GaAs-injured groups, compared to control group. The MyoD gene expression increased (P = 0.008) in the GaAs-injured group, but not in the injured group (P = 0.338), compared to control group. GaAs laser therapy had a positive effect on MyoD gene expression, but not IGF-I, MAFbx, and TNF-α, during intermediary phases (14 days post-injury) of muscle repair. |
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High final energy of gallium arsenide laser increases MyoD gene expression during the intermediate phase of muscle regeneration after cryoinjury in ratsGallium arsenide laserIGF-IMAFbxMuscle regenerationMyoDTNF-α gene expressionThe aim of this study was to determine the effects of gallium arsenide (GaAs) laser on IGF-I, MyoD, MAFbx, and TNF-α gene expression during the intermediate phase of muscle regeneration after cryoinjury 21 Wistar rats were divided into three groups (n = 7 per group): untreated with no injury (control group), cryoinjury without GaAs (injured group), and cryoinjury with GaAs (GaAs-injured group). The cryoinjury was induced in the central region of the tibialis anterior muscle (TA). The region injured was irradiated once a day during 14 days using GaAs laser (904 nm; spot size 0.035 cm2, output power 50 mW; energy density 69 J cm−2; exposure time 4 s per point; final energy 4.8 J). Twenty-four hours after the last application, the right and left TA muscles were collected for histological (collagen content) and molecular (gene expression of IGF-I, MyoD, MAFbx, and TNF-α) analyses, respectively. Data were analyzed using one-way ANOVA at P < 0.05. There were no significant (P > 0.05) differences in collagen density and IGF-I gene expression in all experimental groups. There were similar (P < 0.05) decreases in MAFbx and TNF-α gene expression in the injured and GaAs-injured groups, compared to control group. The MyoD gene expression increased (P = 0.008) in the GaAs-injured group, but not in the injured group (P = 0.338), compared to control group. GaAs laser therapy had a positive effect on MyoD gene expression, but not IGF-I, MAFbx, and TNF-α, during intermediary phases (14 days post-injury) of muscle repair.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Physical Therapy University of Western São Paulo (UNOESTE)Center of Research in Health Science North University of Paraná (UNOPAR), Avenue Paris, 675, Jardim PizaDepartment of Morphology São Paulo State University (UNESP)Department of Morphology São Paulo State University (UNESP)CNPq: 146045/2013-0University of Western São Paulo (UNOESTE)North University of Paraná (UNOPAR)Universidade Estadual Paulista (Unesp)Santos, Caroline PereiraAguiar, Andreo FernandoGiometti, Ines CristinaMariano, Thaoan Brunode Freitas, Carlos Eduardo AssumpçãoNai, Gisele Alborghettide Freitas, Selma ZambelliPai-Silva, Maeli Dal [UNESP]Pacagnelli, Francis Lopes2018-12-11T16:51:39Z2018-12-11T16:51:39Z2018-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article843-850application/pdfhttp://dx.doi.org/10.1007/s10103-018-2439-3Lasers in Medical Science, v. 33, n. 4, p. 843-850, 2018.1435-604X0268-8921http://hdl.handle.net/11449/17060310.1007/s10103-018-2439-32-s2.0-850413500742-s2.0-85041350074.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLasers in Medical Science0,713info:eu-repo/semantics/openAccess2023-10-18T06:04:29Zoai:repositorio.unesp.br:11449/170603Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:13:11.401240Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
High final energy of gallium arsenide laser increases MyoD gene expression during the intermediate phase of muscle regeneration after cryoinjury in rats |
title |
High final energy of gallium arsenide laser increases MyoD gene expression during the intermediate phase of muscle regeneration after cryoinjury in rats |
spellingShingle |
High final energy of gallium arsenide laser increases MyoD gene expression during the intermediate phase of muscle regeneration after cryoinjury in rats Santos, Caroline Pereira Gallium arsenide laser IGF-I MAFbx Muscle regeneration MyoD TNF-α gene expression |
title_short |
High final energy of gallium arsenide laser increases MyoD gene expression during the intermediate phase of muscle regeneration after cryoinjury in rats |
title_full |
High final energy of gallium arsenide laser increases MyoD gene expression during the intermediate phase of muscle regeneration after cryoinjury in rats |
title_fullStr |
High final energy of gallium arsenide laser increases MyoD gene expression during the intermediate phase of muscle regeneration after cryoinjury in rats |
title_full_unstemmed |
High final energy of gallium arsenide laser increases MyoD gene expression during the intermediate phase of muscle regeneration after cryoinjury in rats |
title_sort |
High final energy of gallium arsenide laser increases MyoD gene expression during the intermediate phase of muscle regeneration after cryoinjury in rats |
author |
Santos, Caroline Pereira |
author_facet |
Santos, Caroline Pereira Aguiar, Andreo Fernando Giometti, Ines Cristina Mariano, Thaoan Bruno de Freitas, Carlos Eduardo Assumpção Nai, Gisele Alborghetti de Freitas, Selma Zambelli Pai-Silva, Maeli Dal [UNESP] Pacagnelli, Francis Lopes |
author_role |
author |
author2 |
Aguiar, Andreo Fernando Giometti, Ines Cristina Mariano, Thaoan Bruno de Freitas, Carlos Eduardo Assumpção Nai, Gisele Alborghetti de Freitas, Selma Zambelli Pai-Silva, Maeli Dal [UNESP] Pacagnelli, Francis Lopes |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
University of Western São Paulo (UNOESTE) North University of Paraná (UNOPAR) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Santos, Caroline Pereira Aguiar, Andreo Fernando Giometti, Ines Cristina Mariano, Thaoan Bruno de Freitas, Carlos Eduardo Assumpção Nai, Gisele Alborghetti de Freitas, Selma Zambelli Pai-Silva, Maeli Dal [UNESP] Pacagnelli, Francis Lopes |
dc.subject.por.fl_str_mv |
Gallium arsenide laser IGF-I MAFbx Muscle regeneration MyoD TNF-α gene expression |
topic |
Gallium arsenide laser IGF-I MAFbx Muscle regeneration MyoD TNF-α gene expression |
description |
The aim of this study was to determine the effects of gallium arsenide (GaAs) laser on IGF-I, MyoD, MAFbx, and TNF-α gene expression during the intermediate phase of muscle regeneration after cryoinjury 21 Wistar rats were divided into three groups (n = 7 per group): untreated with no injury (control group), cryoinjury without GaAs (injured group), and cryoinjury with GaAs (GaAs-injured group). The cryoinjury was induced in the central region of the tibialis anterior muscle (TA). The region injured was irradiated once a day during 14 days using GaAs laser (904 nm; spot size 0.035 cm2, output power 50 mW; energy density 69 J cm−2; exposure time 4 s per point; final energy 4.8 J). Twenty-four hours after the last application, the right and left TA muscles were collected for histological (collagen content) and molecular (gene expression of IGF-I, MyoD, MAFbx, and TNF-α) analyses, respectively. Data were analyzed using one-way ANOVA at P < 0.05. There were no significant (P > 0.05) differences in collagen density and IGF-I gene expression in all experimental groups. There were similar (P < 0.05) decreases in MAFbx and TNF-α gene expression in the injured and GaAs-injured groups, compared to control group. The MyoD gene expression increased (P = 0.008) in the GaAs-injured group, but not in the injured group (P = 0.338), compared to control group. GaAs laser therapy had a positive effect on MyoD gene expression, but not IGF-I, MAFbx, and TNF-α, during intermediary phases (14 days post-injury) of muscle repair. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T16:51:39Z 2018-12-11T16:51:39Z 2018-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10103-018-2439-3 Lasers in Medical Science, v. 33, n. 4, p. 843-850, 2018. 1435-604X 0268-8921 http://hdl.handle.net/11449/170603 10.1007/s10103-018-2439-3 2-s2.0-85041350074 2-s2.0-85041350074.pdf |
url |
http://dx.doi.org/10.1007/s10103-018-2439-3 http://hdl.handle.net/11449/170603 |
identifier_str_mv |
Lasers in Medical Science, v. 33, n. 4, p. 843-850, 2018. 1435-604X 0268-8921 10.1007/s10103-018-2439-3 2-s2.0-85041350074 2-s2.0-85041350074.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Lasers in Medical Science 0,713 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
843-850 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128482191867904 |