2-deoxyglucose-induced food intake by Nile tilapia, Oreochromis niloticus (L.).
Autor(a) principal: | |
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Data de Publicação: | 1993 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/219162 |
Resumo: | The alimentary and glycemic responses to cytoglycopenia were studied in thirty-one Nile tilapia alevins of indeterminate sex and age, measuring on average 10.67 +/- 0.82 cm. The cytoglycopenia was provoked by ip injection of 60 mg/kg 2-deoxy-D-glucose (2-DG, N = 16). The control group (N = 15) was submitted to ip injection of 0.2 ml saline. Blood samples for glucose determination were obtained before and three hours after drug administration by cardiac puncture. Food was then offered ad libitum. One hour later the animals were sacrificed and their stomachs removed. The difference in wet weight between full and empty stomach was utilized to quantify the food intake. Median food intake was 0.3877 g for the fish treated with 2-DG and 0.107 g for the animals injected with saline. This difference was statistically significant by the Mann-Whitney test (P < 0.05). The median values of blood glucose levels before drug injection were 46.19 mg/100 ml in the 2-DG-treated fish and 44.54 mg/100 ml in the control group. Three hours after drug administration, the values were 48.64 mg/100 ml in the experimental group and 56.90 mg/100 ml in the control group. The difference between the values of blood glucose before and after the drug was not significant for either group. We conclude that glucoprivation provokes food intake in fish and that the same glucoprivation was not sufficient to provoke hyperglycemia. |
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Repositório Institucional da UNESP |
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spelling |
2-deoxyglucose-induced food intake by Nile tilapia, Oreochromis niloticus (L.).The alimentary and glycemic responses to cytoglycopenia were studied in thirty-one Nile tilapia alevins of indeterminate sex and age, measuring on average 10.67 +/- 0.82 cm. The cytoglycopenia was provoked by ip injection of 60 mg/kg 2-deoxy-D-glucose (2-DG, N = 16). The control group (N = 15) was submitted to ip injection of 0.2 ml saline. Blood samples for glucose determination were obtained before and three hours after drug administration by cardiac puncture. Food was then offered ad libitum. One hour later the animals were sacrificed and their stomachs removed. The difference in wet weight between full and empty stomach was utilized to quantify the food intake. Median food intake was 0.3877 g for the fish treated with 2-DG and 0.107 g for the animals injected with saline. This difference was statistically significant by the Mann-Whitney test (P < 0.05). The median values of blood glucose levels before drug injection were 46.19 mg/100 ml in the 2-DG-treated fish and 44.54 mg/100 ml in the control group. Three hours after drug administration, the values were 48.64 mg/100 ml in the experimental group and 56.90 mg/100 ml in the control group. The difference between the values of blood glucose before and after the drug was not significant for either group. We conclude that glucoprivation provokes food intake in fish and that the same glucoprivation was not sufficient to provoke hyperglycemia.Departamento de Fisiologia Universidade Estadual Paulista Júlio de Mesquita Filho SPDepartamento de Fisiologia Universidade Estadual Paulista Júlio de Mesquita Filho SPUniversidade Estadual Paulista (UNESP)Delicio, H. C. [UNESP]Vicentini-Paulino, M. L. [UNESP]2022-04-28T18:54:03Z2022-04-28T18:54:03Z1993-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article327-331Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.], v. 26, n. 3, p. 327-331, 1993.0100-879Xhttp://hdl.handle.net/11449/2191622-s2.0-0027552932Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.]info:eu-repo/semantics/openAccess2022-04-28T18:54:03Zoai:repositorio.unesp.br:11449/219162Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:39:00.146873Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
2-deoxyglucose-induced food intake by Nile tilapia, Oreochromis niloticus (L.). |
title |
2-deoxyglucose-induced food intake by Nile tilapia, Oreochromis niloticus (L.). |
spellingShingle |
2-deoxyglucose-induced food intake by Nile tilapia, Oreochromis niloticus (L.). Delicio, H. C. [UNESP] |
title_short |
2-deoxyglucose-induced food intake by Nile tilapia, Oreochromis niloticus (L.). |
title_full |
2-deoxyglucose-induced food intake by Nile tilapia, Oreochromis niloticus (L.). |
title_fullStr |
2-deoxyglucose-induced food intake by Nile tilapia, Oreochromis niloticus (L.). |
title_full_unstemmed |
2-deoxyglucose-induced food intake by Nile tilapia, Oreochromis niloticus (L.). |
title_sort |
2-deoxyglucose-induced food intake by Nile tilapia, Oreochromis niloticus (L.). |
author |
Delicio, H. C. [UNESP] |
author_facet |
Delicio, H. C. [UNESP] Vicentini-Paulino, M. L. [UNESP] |
author_role |
author |
author2 |
Vicentini-Paulino, M. L. [UNESP] |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Delicio, H. C. [UNESP] Vicentini-Paulino, M. L. [UNESP] |
description |
The alimentary and glycemic responses to cytoglycopenia were studied in thirty-one Nile tilapia alevins of indeterminate sex and age, measuring on average 10.67 +/- 0.82 cm. The cytoglycopenia was provoked by ip injection of 60 mg/kg 2-deoxy-D-glucose (2-DG, N = 16). The control group (N = 15) was submitted to ip injection of 0.2 ml saline. Blood samples for glucose determination were obtained before and three hours after drug administration by cardiac puncture. Food was then offered ad libitum. One hour later the animals were sacrificed and their stomachs removed. The difference in wet weight between full and empty stomach was utilized to quantify the food intake. Median food intake was 0.3877 g for the fish treated with 2-DG and 0.107 g for the animals injected with saline. This difference was statistically significant by the Mann-Whitney test (P < 0.05). The median values of blood glucose levels before drug injection were 46.19 mg/100 ml in the 2-DG-treated fish and 44.54 mg/100 ml in the control group. Three hours after drug administration, the values were 48.64 mg/100 ml in the experimental group and 56.90 mg/100 ml in the control group. The difference between the values of blood glucose before and after the drug was not significant for either group. We conclude that glucoprivation provokes food intake in fish and that the same glucoprivation was not sufficient to provoke hyperglycemia. |
publishDate |
1993 |
dc.date.none.fl_str_mv |
1993-01-01 2022-04-28T18:54:03Z 2022-04-28T18:54:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.], v. 26, n. 3, p. 327-331, 1993. 0100-879X http://hdl.handle.net/11449/219162 2-s2.0-0027552932 |
identifier_str_mv |
Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.], v. 26, n. 3, p. 327-331, 1993. 0100-879X 2-s2.0-0027552932 |
url |
http://hdl.handle.net/11449/219162 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.] |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
327-331 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129343873875968 |