Resistência antimicrobiana de Staphylococcus aureus isolados de produtos de origem animal

Detalhes bibliográficos
Autor(a) principal: VASCONCELOS, Cristiane de Melo
Data de Publicação: 2018
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRPE
Texto Completo: http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/7653
Resumo: Staphylococcus aureus is a commensal microorganism of the skin and mucous membranes of animals and humans and is considered an opportunistic agent responsible for food poisoning. Another threat to public health relates to its antimicrobial resistance capacity, which has been detected in isolated strains of various animal products and is associated with the indiscriminate use of antimicrobial agents in animal production, which warns of the transmission of this microorganism through food. The objective of this study was to investigate the occurrence of antimicrobial resistant Staphylococcus aureus in handmade coalho cheese, beef ground and pork and chicken meat. From each animal product, 3 market samples were acquired in Pernambuco, Brazil, which were submitted to microbiological analysis for coagulase positive Staphylococcus and S. aureus counts. S. aureus strains were isolated, submitted to the antimicrobial susceptibility test to nine antimicrobials used in the human and veterinary clinics, and the antimicrobial multiresistance was then evaluated and the Multiple Antimicrobial Resistance (MAR) index was calculated. Contamination in all types of products was verified by S. coagulase positive, with an equal count for S. aureus, ranging from 2.4 x 103 CFU/g in the pork meat to 2.2 x 106 CFU/g in chicken meat. Among the isolated strains, 37.5% (45/120) were confirmed as S. aureus, with the highest occurrence in pork with 63% (19/45) and handmade coalho cheese with 37% (14/45), followed by ground beef with 33% (10/45) and chicken meat with 6.7% (2/45). Regarding antimicrobial resistance, pork meat, followed by handmade coalho cheese and chicken meat had the highest number of resistant strains. There was a higher resistance to tetracycline, with 33.3% (15/45) of S. aureus strains, followed by penicillin G with 24.4% (11/45), erythromycin with 8.8% (4/45), clindamycin with 6.6% (3/45) and cefoxitin with 2.2% (1/45), with 4 (8.8%) strains considered multidrug-resistant (MDR), 3 (6.6%) MLSc (constitutive resistance to macrolide, lincosamide and streptogramin), 1 (2.2%) MRSA (methicillin-resistant S. aureus) and 17 (37.7%) from potential sources of high-risk contamination, with MAR index ≥ 0.2. There was no resistance to gentamicin, ciprofloxacin, chloramphenicol and sulfazotrim. It is concluded that the products of animal origin analyzed showed contamination by S. aureus and may be reservoirs of strains resistant to antimicrobials, representing a potential risk to the health of the population.
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spelling MACHADO, Erilane de Castro LimaSILVA, Michelle Rose de OliveiraSILVA, Celiane Gomes Maia daXAVIER, Danilo EliasMORAIS, Márcia Maria Camargohttp://lattes.cnpq.br/6288561678724837VASCONCELOS, Cristiane de Melo2018-10-19T13:53:41Z2018-08-31VASCONCELOS, Cristiane de Melo. Resistência antimicrobiana de Staphylococcus aureus isolados de produtos de origem animal. 2018. 79 f. Dissertação (Programa de Pós-Graduação em Ciência e Tecnologia de Alimentos) - Universidade Federal Rural de Pernambuco, Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/7653Staphylococcus aureus is a commensal microorganism of the skin and mucous membranes of animals and humans and is considered an opportunistic agent responsible for food poisoning. Another threat to public health relates to its antimicrobial resistance capacity, which has been detected in isolated strains of various animal products and is associated with the indiscriminate use of antimicrobial agents in animal production, which warns of the transmission of this microorganism through food. The objective of this study was to investigate the occurrence of antimicrobial resistant Staphylococcus aureus in handmade coalho cheese, beef ground and pork and chicken meat. From each animal product, 3 market samples were acquired in Pernambuco, Brazil, which were submitted to microbiological analysis for coagulase positive Staphylococcus and S. aureus counts. S. aureus strains were isolated, submitted to the antimicrobial susceptibility test to nine antimicrobials used in the human and veterinary clinics, and the antimicrobial multiresistance was then evaluated and the Multiple Antimicrobial Resistance (MAR) index was calculated. Contamination in all types of products was verified by S. coagulase positive, with an equal count for S. aureus, ranging from 2.4 x 103 CFU/g in the pork meat to 2.2 x 106 CFU/g in chicken meat. Among the isolated strains, 37.5% (45/120) were confirmed as S. aureus, with the highest occurrence in pork with 63% (19/45) and handmade coalho cheese with 37% (14/45), followed by ground beef with 33% (10/45) and chicken meat with 6.7% (2/45). Regarding antimicrobial resistance, pork meat, followed by handmade coalho cheese and chicken meat had the highest number of resistant strains. There was a higher resistance to tetracycline, with 33.3% (15/45) of S. aureus strains, followed by penicillin G with 24.4% (11/45), erythromycin with 8.8% (4/45), clindamycin with 6.6% (3/45) and cefoxitin with 2.2% (1/45), with 4 (8.8%) strains considered multidrug-resistant (MDR), 3 (6.6%) MLSc (constitutive resistance to macrolide, lincosamide and streptogramin), 1 (2.2%) MRSA (methicillin-resistant S. aureus) and 17 (37.7%) from potential sources of high-risk contamination, with MAR index ≥ 0.2. There was no resistance to gentamicin, ciprofloxacin, chloramphenicol and sulfazotrim. It is concluded that the products of animal origin analyzed showed contamination by S. aureus and may be reservoirs of strains resistant to antimicrobials, representing a potential risk to the health of the population.Staphylococcus aureus é um microrganismo comensal da pele e de membranas mucosas de animais e de seres humanos, sendo considerado um agente oportunista e responsável por intoxicação alimentar. Uma outra ameaça à saúde pública relaciona-se à sua capacidade de resistência antimicrobiana, que tem sido detectada em cepas isoladas de diversos produtos de origem animal e associa-se ao uso indiscriminado de agentes antimicrobianos na produção animal, o que alerta sobre o monitoramento da transmissão deste microrganismo através de alimentos. Objetivou-se com o presente estudo pesquisar a ocorrência de Staphylococcus aureus resistentes a antimicrobianos em queijo de coalho artesanal, carne moída bovina e carnes suína e de frango. De cada produto de origem animal, foram adquiridas 3 amostras de minimercados em Pernambuco, Brasil, que foram submetidas à análise microbiológica para contagem de Staphylococcus coagulase positiva e de S. aureus. Cepas de S. aureus foram isoladas, submetidas ao teste de susceptibilidade antimicrobiana a nove antimicrobianos utilizados nas clínicas humana e veterinária e, posteriormente, avaliou-se a multirresistência antimicrobiana e calculou-se o índice de Múltipla Resistência Antimicrobiana (MAR). Verificou-se contaminação em todos os tipos de produtos por S. coagulase positiva, com igual contagem para S. aureus, variando de 2,4 x 103 UFC/g na carne suína a 2,2 x 106 UFC/g na carne de frango. Entre as cepas isoladas, 37,5% (45/120) foram confirmadas como S. aureus, em maior ocorrência na carne suína com 63% (19/45) e no queijo de coalho artesanal com 37% (14/45), seguidos da carne moída bovina com 33% (10/45) e da carne de frango com 6,7% (2/45). Quanto à resistência antimicrobiana, a carne suína, seguida do queijo de coalho e da carne de frango apresentaram o maior número de cepas resistentes. Houve maior resistência à tetraciclina, com 33,3% (15/45) das cepas de S. aureus, seguida por penicilina G com 24,4% (11/45), eritromicina com 8,8% (4/45), clindamicina com 6,6% (3/45) e cefoxitina com 2,2% (1/45), sendo 4 (8,8%) cepas consideradas multidrogarresistentes (MDR), 3 (6,6%) MLSc (resistência constitutiva aos macrolídeos, lincosamidas e estreptogramina), 1 (2,2%) MRSA (S. aureus resistente à meticilina) e 17 (37,7%) provenientes de potenciais fontes de contaminação de alto risco, com índice MAR ≥ 0,2. Não houve resistência à gentamicina, ciprofloxacina, cloranfenicol e sulfazotrim. Conclui-se que os produtos de origem animal analisados apresentaram contaminação por S. aureus e podem ser reservatórios de cepas resistentes a antimicrobianos, representando um potencial risco à saúde da população.Submitted by Mario BC (mario@bc.ufrpe.br) on 2018-10-19T13:53:41Z No. of bitstreams: 1 Cristiane de Melo Vasconcelos.pdf: 745658 bytes, checksum: f6e135c1790e48a3d88b17616bf80d1d (MD5)Made available in DSpace on 2018-10-19T13:53:41Z (GMT). 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dc.title.por.fl_str_mv Resistência antimicrobiana de Staphylococcus aureus isolados de produtos de origem animal
title Resistência antimicrobiana de Staphylococcus aureus isolados de produtos de origem animal
spellingShingle Resistência antimicrobiana de Staphylococcus aureus isolados de produtos de origem animal
VASCONCELOS, Cristiane de Melo
Staphylococcus aureus
Antimicrobiano
Microrganismo
CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS
title_short Resistência antimicrobiana de Staphylococcus aureus isolados de produtos de origem animal
title_full Resistência antimicrobiana de Staphylococcus aureus isolados de produtos de origem animal
title_fullStr Resistência antimicrobiana de Staphylococcus aureus isolados de produtos de origem animal
title_full_unstemmed Resistência antimicrobiana de Staphylococcus aureus isolados de produtos de origem animal
title_sort Resistência antimicrobiana de Staphylococcus aureus isolados de produtos de origem animal
author VASCONCELOS, Cristiane de Melo
author_facet VASCONCELOS, Cristiane de Melo
author_role author
dc.contributor.advisor1.fl_str_mv MACHADO, Erilane de Castro Lima
dc.contributor.advisor-co1.fl_str_mv SILVA, Michelle Rose de Oliveira
dc.contributor.referee1.fl_str_mv SILVA, Celiane Gomes Maia da
dc.contributor.referee2.fl_str_mv XAVIER, Danilo Elias
dc.contributor.referee3.fl_str_mv MORAIS, Márcia Maria Camargo
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/6288561678724837
dc.contributor.author.fl_str_mv VASCONCELOS, Cristiane de Melo
contributor_str_mv MACHADO, Erilane de Castro Lima
SILVA, Michelle Rose de Oliveira
SILVA, Celiane Gomes Maia da
XAVIER, Danilo Elias
MORAIS, Márcia Maria Camargo
dc.subject.por.fl_str_mv Staphylococcus aureus
Antimicrobiano
Microrganismo
topic Staphylococcus aureus
Antimicrobiano
Microrganismo
CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS
dc.subject.cnpq.fl_str_mv CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS
description Staphylococcus aureus is a commensal microorganism of the skin and mucous membranes of animals and humans and is considered an opportunistic agent responsible for food poisoning. Another threat to public health relates to its antimicrobial resistance capacity, which has been detected in isolated strains of various animal products and is associated with the indiscriminate use of antimicrobial agents in animal production, which warns of the transmission of this microorganism through food. The objective of this study was to investigate the occurrence of antimicrobial resistant Staphylococcus aureus in handmade coalho cheese, beef ground and pork and chicken meat. From each animal product, 3 market samples were acquired in Pernambuco, Brazil, which were submitted to microbiological analysis for coagulase positive Staphylococcus and S. aureus counts. S. aureus strains were isolated, submitted to the antimicrobial susceptibility test to nine antimicrobials used in the human and veterinary clinics, and the antimicrobial multiresistance was then evaluated and the Multiple Antimicrobial Resistance (MAR) index was calculated. Contamination in all types of products was verified by S. coagulase positive, with an equal count for S. aureus, ranging from 2.4 x 103 CFU/g in the pork meat to 2.2 x 106 CFU/g in chicken meat. Among the isolated strains, 37.5% (45/120) were confirmed as S. aureus, with the highest occurrence in pork with 63% (19/45) and handmade coalho cheese with 37% (14/45), followed by ground beef with 33% (10/45) and chicken meat with 6.7% (2/45). Regarding antimicrobial resistance, pork meat, followed by handmade coalho cheese and chicken meat had the highest number of resistant strains. There was a higher resistance to tetracycline, with 33.3% (15/45) of S. aureus strains, followed by penicillin G with 24.4% (11/45), erythromycin with 8.8% (4/45), clindamycin with 6.6% (3/45) and cefoxitin with 2.2% (1/45), with 4 (8.8%) strains considered multidrug-resistant (MDR), 3 (6.6%) MLSc (constitutive resistance to macrolide, lincosamide and streptogramin), 1 (2.2%) MRSA (methicillin-resistant S. aureus) and 17 (37.7%) from potential sources of high-risk contamination, with MAR index ≥ 0.2. There was no resistance to gentamicin, ciprofloxacin, chloramphenicol and sulfazotrim. It is concluded that the products of animal origin analyzed showed contamination by S. aureus and may be reservoirs of strains resistant to antimicrobials, representing a potential risk to the health of the population.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-10-19T13:53:41Z
dc.date.issued.fl_str_mv 2018-08-31
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.citation.fl_str_mv VASCONCELOS, Cristiane de Melo. Resistência antimicrobiana de Staphylococcus aureus isolados de produtos de origem animal. 2018. 79 f. Dissertação (Programa de Pós-Graduação em Ciência e Tecnologia de Alimentos) - Universidade Federal Rural de Pernambuco, Recife.
dc.identifier.uri.fl_str_mv http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/7653
identifier_str_mv VASCONCELOS, Cristiane de Melo. Resistência antimicrobiana de Staphylococcus aureus isolados de produtos de origem animal. 2018. 79 f. Dissertação (Programa de Pós-Graduação em Ciência e Tecnologia de Alimentos) - Universidade Federal Rural de Pernambuco, Recife.
url http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/7653
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dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Departamento de Ciências Domésticas
publisher.none.fl_str_mv Universidade Federal Rural de Pernambuco
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