Análise epidemiológica da infecção por Mycoplasma bovigenitalium e Ureaplasma diversum em bovinos na microrregião do Vale do Ipanema - Pernambuco

Detalhes bibliográficos
Autor(a) principal: MACÊDO, Allison Alves de
Data de Publicação: 2017
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRPE
Texto Completo: http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/7306
Resumo: This study’s objective was to conduct a Mycoplasma bovigenitalium and Ureaplasma diversum infections epidemiological investigation in cattle in the Ipanema Valley microregion, Pernambuco state. Vaginal swabs were collected from 355 reproductive age cows and submitted to polymerase chain reaction (PCR) and microbiological isolation. An investigative questionnaire was used to risk factors analysis associated with Mollicutes infection. According to PCR, 9.29% (33/355) of the samples were positive for Mycoplasma bovigenitalium and 21.69% (77/355) for Ureaplasma diversum. Coinfection was observed in 2.81% (10/355) of the samples. According to isolation, there was growth on Hayflick medium of 81.81% (27/33) from the samples classified as Mycoplasma spp. and 24.67% (19/77) growth on UB medium, being classified as Ureaplasma diversum. Risk factors associated to Mollicutes infection were considered: semi-intensive breeding system (OR = 4.6), pasture rent (OR = 3.6), reproductive disorders nonisolated animals (OR = 3.2), and natural mating plus artificial insemination (OR = 3.5). This is the first record of Mycoplasma bovigenitalium and Ureaplasma diversum infection in semiarid region cattle herds of Pernambuco state, Brazil. From this, preventive measures directed to the identified risk factors can contribute to Mollicutes’ occurrence decrease in the cattle herds studied.
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spelling PINHEIRO JUNIOR, José WiltonSANTOS, Sandra Batista dosPINHEIRO JUNIOR, José WiltonMOTA, Rinaldo AparecidoSANTOS, Sandra Batista doshttp://lattes.cnpq.br/3932177528242791MACÊDO, Allison Alves de2018-06-19T12:55:47Z2017-02-06MACÊDO, Allison Alves de. Análise epidemiológica da infecção por Mycoplasma bovigenitalium e Ureaplasma diversum em bovinos na microrregião do Vale do Ipanema - Pernambuco. 2017. 57 f. Dissertação (Programa de Pós-Graduação em Ciência Animal Tropical) - Universidade Federal Rural de Pernambuco, Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/7306This study’s objective was to conduct a Mycoplasma bovigenitalium and Ureaplasma diversum infections epidemiological investigation in cattle in the Ipanema Valley microregion, Pernambuco state. Vaginal swabs were collected from 355 reproductive age cows and submitted to polymerase chain reaction (PCR) and microbiological isolation. An investigative questionnaire was used to risk factors analysis associated with Mollicutes infection. According to PCR, 9.29% (33/355) of the samples were positive for Mycoplasma bovigenitalium and 21.69% (77/355) for Ureaplasma diversum. Coinfection was observed in 2.81% (10/355) of the samples. According to isolation, there was growth on Hayflick medium of 81.81% (27/33) from the samples classified as Mycoplasma spp. and 24.67% (19/77) growth on UB medium, being classified as Ureaplasma diversum. Risk factors associated to Mollicutes infection were considered: semi-intensive breeding system (OR = 4.6), pasture rent (OR = 3.6), reproductive disorders nonisolated animals (OR = 3.2), and natural mating plus artificial insemination (OR = 3.5). This is the first record of Mycoplasma bovigenitalium and Ureaplasma diversum infection in semiarid region cattle herds of Pernambuco state, Brazil. From this, preventive measures directed to the identified risk factors can contribute to Mollicutes’ occurrence decrease in the cattle herds studied.Objetivou-se, com este estudo, realizar uma investigação epidemiológica da infecção por Mycoplasma bovigenitalium e Ureaplasma diversum em bovinos na microrregião do Vale do Ipanema, estado de Pernambuco. Foram coletados suabes vaginais de 355 vacas em idade reprodutiva que foram submetidos à Reação em Cadeia da Polimerase (PCR) e isolamento microbiológico. Um questionário investigativo foi utilizado para a análise dos fatores de risco associados à infecção por Mollicutes. Pela PCR, 9,29% (33/355) amostras foram positivas para Mycoplasma bovigenitalium e 21,69% (77/355) para Ureaplasma diversum. Observou-se coinfecção em 2,81% (10/355) das amostras. A partir do isolamento microbiológico das amostras positivas na PCR, houve crescimento no meio Hayflick de 81,81% (27/33) das amostras, sendo classificadas como Mycoplasma spp. e 24,67% (19/77) cresceram no meio “UB”, sendo classificadas como U. diversum. Foram considerados fatores de risco associados à infecção por Mollicutes: sistema de criação semi-intensivo (OR=4,6); aluguel de pastagens (OR=3,6); não isolamento dos animais com distúrbios reprodutivos (OR=3,2) e realização de monta natural mais inseminação artificial (OR=3,5). Este é o primeiro registro da infecção por Mycoplasma bovigenitalium e Ureaplasma diversum em rebanhos bovinos na região semiárida do estado de Pernambuco, Brasil. A partir dos resultados obtidos, medidas de prevenção direcionadas aos fatores de riscos identificados podem contribuir com a diminuição da ocorrência de Mollicutes nos rebanhos bovinos estudados.Submitted by Mario BC (mario@bc.ufrpe.br) on 2018-06-19T12:55:46Z No. of bitstreams: 1 Allison Alves de Macedo.pdf: 650201 bytes, checksum: b68962f58a5ff7ccd111367548d6a064 (MD5)Made available in DSpace on 2018-06-19T12:55:47Z (GMT). No. of bitstreams: 1 Allison Alves de Macedo.pdf: 650201 bytes, checksum: b68962f58a5ff7ccd111367548d6a064 (MD5) Previous issue date: 2017-02-06application/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em Ciência Animal TropicalUFRPEBrasilDepartamento de Morfologia e Fisiologia AnimalEpidemiologiaMycoplasma bovigenitaliumUreaplasma diversumBovinoCIENCIAS AGRARIAS::MEDICINA VETERINARIAAnálise epidemiológica da infecção por Mycoplasma bovigenitalium e Ureaplasma diversum em bovinos na microrregião do Vale do Ipanema - Pernambucoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-6143187600765506511600600600-8922364187987396204453670264235017319info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPEORIGINALAllison Alves de Macedo.pdfAllison Alves de Macedo.pdfapplication/pdf650201http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/7306/2/Allison+Alves+de+Macedo.pdfb68962f58a5ff7ccd111367548d6a064MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/7306/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede2/73062018-06-19 09:55:47.027oai:tede2: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Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2018-06-19T12:55:47Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false
dc.title.por.fl_str_mv Análise epidemiológica da infecção por Mycoplasma bovigenitalium e Ureaplasma diversum em bovinos na microrregião do Vale do Ipanema - Pernambuco
title Análise epidemiológica da infecção por Mycoplasma bovigenitalium e Ureaplasma diversum em bovinos na microrregião do Vale do Ipanema - Pernambuco
spellingShingle Análise epidemiológica da infecção por Mycoplasma bovigenitalium e Ureaplasma diversum em bovinos na microrregião do Vale do Ipanema - Pernambuco
MACÊDO, Allison Alves de
Epidemiologia
Mycoplasma bovigenitalium
Ureaplasma diversum
Bovino
CIENCIAS AGRARIAS::MEDICINA VETERINARIA
title_short Análise epidemiológica da infecção por Mycoplasma bovigenitalium e Ureaplasma diversum em bovinos na microrregião do Vale do Ipanema - Pernambuco
title_full Análise epidemiológica da infecção por Mycoplasma bovigenitalium e Ureaplasma diversum em bovinos na microrregião do Vale do Ipanema - Pernambuco
title_fullStr Análise epidemiológica da infecção por Mycoplasma bovigenitalium e Ureaplasma diversum em bovinos na microrregião do Vale do Ipanema - Pernambuco
title_full_unstemmed Análise epidemiológica da infecção por Mycoplasma bovigenitalium e Ureaplasma diversum em bovinos na microrregião do Vale do Ipanema - Pernambuco
title_sort Análise epidemiológica da infecção por Mycoplasma bovigenitalium e Ureaplasma diversum em bovinos na microrregião do Vale do Ipanema - Pernambuco
author MACÊDO, Allison Alves de
author_facet MACÊDO, Allison Alves de
author_role author
dc.contributor.advisor1.fl_str_mv PINHEIRO JUNIOR, José Wilton
dc.contributor.advisor-co1.fl_str_mv SANTOS, Sandra Batista dos
dc.contributor.referee1.fl_str_mv PINHEIRO JUNIOR, José Wilton
dc.contributor.referee2.fl_str_mv MOTA, Rinaldo Aparecido
dc.contributor.referee3.fl_str_mv SANTOS, Sandra Batista dos
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/3932177528242791
dc.contributor.author.fl_str_mv MACÊDO, Allison Alves de
contributor_str_mv PINHEIRO JUNIOR, José Wilton
SANTOS, Sandra Batista dos
PINHEIRO JUNIOR, José Wilton
MOTA, Rinaldo Aparecido
SANTOS, Sandra Batista dos
dc.subject.por.fl_str_mv Epidemiologia
Mycoplasma bovigenitalium
Ureaplasma diversum
Bovino
topic Epidemiologia
Mycoplasma bovigenitalium
Ureaplasma diversum
Bovino
CIENCIAS AGRARIAS::MEDICINA VETERINARIA
dc.subject.cnpq.fl_str_mv CIENCIAS AGRARIAS::MEDICINA VETERINARIA
description This study’s objective was to conduct a Mycoplasma bovigenitalium and Ureaplasma diversum infections epidemiological investigation in cattle in the Ipanema Valley microregion, Pernambuco state. Vaginal swabs were collected from 355 reproductive age cows and submitted to polymerase chain reaction (PCR) and microbiological isolation. An investigative questionnaire was used to risk factors analysis associated with Mollicutes infection. According to PCR, 9.29% (33/355) of the samples were positive for Mycoplasma bovigenitalium and 21.69% (77/355) for Ureaplasma diversum. Coinfection was observed in 2.81% (10/355) of the samples. According to isolation, there was growth on Hayflick medium of 81.81% (27/33) from the samples classified as Mycoplasma spp. and 24.67% (19/77) growth on UB medium, being classified as Ureaplasma diversum. Risk factors associated to Mollicutes infection were considered: semi-intensive breeding system (OR = 4.6), pasture rent (OR = 3.6), reproductive disorders nonisolated animals (OR = 3.2), and natural mating plus artificial insemination (OR = 3.5). This is the first record of Mycoplasma bovigenitalium and Ureaplasma diversum infection in semiarid region cattle herds of Pernambuco state, Brazil. From this, preventive measures directed to the identified risk factors can contribute to Mollicutes’ occurrence decrease in the cattle herds studied.
publishDate 2017
dc.date.issued.fl_str_mv 2017-02-06
dc.date.accessioned.fl_str_mv 2018-06-19T12:55:47Z
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dc.identifier.citation.fl_str_mv MACÊDO, Allison Alves de. Análise epidemiológica da infecção por Mycoplasma bovigenitalium e Ureaplasma diversum em bovinos na microrregião do Vale do Ipanema - Pernambuco. 2017. 57 f. Dissertação (Programa de Pós-Graduação em Ciência Animal Tropical) - Universidade Federal Rural de Pernambuco, Recife.
dc.identifier.uri.fl_str_mv http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/7306
identifier_str_mv MACÊDO, Allison Alves de. Análise epidemiológica da infecção por Mycoplasma bovigenitalium e Ureaplasma diversum em bovinos na microrregião do Vale do Ipanema - Pernambuco. 2017. 57 f. Dissertação (Programa de Pós-Graduação em Ciência Animal Tropical) - Universidade Federal Rural de Pernambuco, Recife.
url http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/7306
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dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Ciência Animal Tropical
dc.publisher.initials.fl_str_mv UFRPE
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Departamento de Morfologia e Fisiologia Animal
publisher.none.fl_str_mv Universidade Federal Rural de Pernambuco
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