Diversidade e caracterização de rizóbios associados ao feijão-fava no semiárido

Detalhes bibliográficos
Autor(a) principal: NASCIMENTO, Antunes Romeu Lima do
Data de Publicação: 2014
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRPE
Texto Completo: http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6937
Resumo: The lima bean is a legume producer of edible grains with high protein value, cultivated worldwide, having in Brazil, the Northeast region as the main producer. In this region, the production is made with low use of technology, resulting in low yields. Among the technologies used to increase the production of legume, we can highlight the inoculation of seed with diazotrophic bacterias, with the objective of replace or reduce nitrogen fertilization. The aim of this study was to evaluate the diversity of rhizobia in symbiosis with the lima bean adapted to semiarid conditions. To obtain the isolated was used 02 accessions of lima bean (one of indeterminate growth and other of determinate growth) and 12 composite samples of soil from different mesoregions the states of Paraíba and Pernambuco. Plants were cultived in a protected area with transparent canvas, in design completely randomized in factorial of 12 (soil) x two (accessions) in Leonard pots adapted with plastic bottle containing 1.3 L of sand and 100 mL soil, with three replicates, for 55 days. The variables analyzed were: number of nodules (NN), fresh mass of nodules (MFN), specifies biomass of nodules (BEN), dry mass of the part aerial (MSPA) and dry mass of root (MSR). Data were analyzed using the F test and the means compared by the Scott- Knott test. Of the collected nodules were isolated bacteria and determined their morphological and physiological characteristics in culture medium, in the laboratory of Soil Microbiology of UFRPE (Recife-PE). With the characteristics of the isolates was calculated the coefficient J and was performed the grouping of the bacteria. The soil of Alagoa Grande-PB showed the highest values for NN and MFN, however when analyzing the specific biomass of nodules no detectable differences. For MSR obtained only difference between accessions. And for MSPA differences were observed for both factors, emphasizing the soil of Alagoa Grande-PB, and two soils of native vegetation, Areia-PB and Garanhuns-PE, these being a possible source of efficient strains. The grouping of the bacteria showed that accession of growth determined formed 13 groups, while the other accession, 24. And analyzing all bacteria in one dendrogram were formed groups with bacteria of each separate accession and also united. Accessions differ in groups of rhizobia able to form symbiosis.
id URPE_9d34d189aa4a3cb0bf991bfdf4251cca
oai_identifier_str oai:tede2:tede2/6937
network_acronym_str URPE
network_name_str Biblioteca Digital de Teses e Dissertações da UFRPE
repository_id_str
spelling SANTOS, Carolina Etienne de Rosália e SilvaFREITAS, Ana Dolores Santiago deCOSTA, Cynthia Maria CarneiroPRAGANA, Rossana BarbosaSILVA, Maria de Fátima dahttp://lattes.cnpq.br/9892727812146615NASCIMENTO, Antunes Romeu Lima do2017-05-22T13:26:07Z2014-02-24NASCIMENTO, Antunes Romeu Lima do. Diversidade e caracterização de rizóbios associados ao feijão-fava no semiárido. 2014. 53 f. Dissertação (Programa de Pós-Graduação em Produção Vegetal) - Universidade Federal Rural de Pernambuco, Serra Talhada.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6937The lima bean is a legume producer of edible grains with high protein value, cultivated worldwide, having in Brazil, the Northeast region as the main producer. In this region, the production is made with low use of technology, resulting in low yields. Among the technologies used to increase the production of legume, we can highlight the inoculation of seed with diazotrophic bacterias, with the objective of replace or reduce nitrogen fertilization. The aim of this study was to evaluate the diversity of rhizobia in symbiosis with the lima bean adapted to semiarid conditions. To obtain the isolated was used 02 accessions of lima bean (one of indeterminate growth and other of determinate growth) and 12 composite samples of soil from different mesoregions the states of Paraíba and Pernambuco. Plants were cultived in a protected area with transparent canvas, in design completely randomized in factorial of 12 (soil) x two (accessions) in Leonard pots adapted with plastic bottle containing 1.3 L of sand and 100 mL soil, with three replicates, for 55 days. The variables analyzed were: number of nodules (NN), fresh mass of nodules (MFN), specifies biomass of nodules (BEN), dry mass of the part aerial (MSPA) and dry mass of root (MSR). Data were analyzed using the F test and the means compared by the Scott- Knott test. Of the collected nodules were isolated bacteria and determined their morphological and physiological characteristics in culture medium, in the laboratory of Soil Microbiology of UFRPE (Recife-PE). With the characteristics of the isolates was calculated the coefficient J and was performed the grouping of the bacteria. The soil of Alagoa Grande-PB showed the highest values for NN and MFN, however when analyzing the specific biomass of nodules no detectable differences. For MSR obtained only difference between accessions. And for MSPA differences were observed for both factors, emphasizing the soil of Alagoa Grande-PB, and two soils of native vegetation, Areia-PB and Garanhuns-PE, these being a possible source of efficient strains. The grouping of the bacteria showed that accession of growth determined formed 13 groups, while the other accession, 24. And analyzing all bacteria in one dendrogram were formed groups with bacteria of each separate accession and also united. Accessions differ in groups of rhizobia able to form symbiosis.O feijão-fava é uma leguminosa produtora de grãos comestíveis com alto valor protéico, cultivada mundialmente, tendo no Brasil, a região Nordeste como principal produtora. Nessa região, a produção é feita com baixa utilização de tecnologia, o que resulta em baixos rendimentos. Entre as tecnologias utilizadas para aumentar a produção de leguminosas, pode-se destacar a inoculação das sementes com bactérias diazotróficas, com o objetivo de substituir ou reduzir a adubação nitrogenada. O objetivo do presente estudo foi avaliar a diversidade de rizóbios em simbiose com o feijão-fava adaptados às condições do Semiárido. Para a obtenção dos isolados foram utilizados dois acessos de feijão-fava (um de crescimento indeterminado e outro de crescimento determinado) e 12 amostras compostas de solo de diferentes mesorregiões dos estados da Paraíba e Pernambuco. As plantas foram cultivadas em uma área protegida com lona transparente, em delineamento experimental inteiramente casualizado em esquema fatorial de 12 (solos) x dois (acessos) em vasos de Leonard adaptados com garrafas PET contendo 1,3 L de areia e 100 mL de solo, com três repetições, por 55 dias. As variáveis analisadas foram: número de nódulos (NN), massa fresca de nódulos (MFN), biomassa especifica dos nódulos (BEN), massa seca da parte aérea (MSPA) e da raiz (MSR). Os dados foram submetidos ao teste F e as médias comparadas pelo teste de Scott-Knott. Dos nódulos coletados foram isoladas bactérias e determinadas suas características morfofisiológicas em meio de cultura, no laboratório de Microbiologia do Solo da UFRPE (Recife-PE). Com as características dos isolados calculou-se o coeficiente J e realizou o agrupamento das bactérias. O solo de Alagoa Grande-PB foi o que apresentou os maiores valores de NN e MFN, entretanto quando analisada a biomassa especifica dos nódulos não se evidenciou diferenças. Para MSR obteve-se diferença apenas entre os acessos. Para MSPA foram observadas diferenças para ambos os fatores, destacando-se o solo de Alagoa Grande-PB, e dois solos de vegetação nativa, Areia-PB e Garanhuns-PE, sendo estes uma possível fonte de estirpes eficientes. O agrupamento das bactérias revelou que o acesso de crescimento determinado formou 13 grupos, enquanto o outro acesso, 24. Analisando-se todas as bactérias em um único dendrograma observou-se a formação de grupos com as bactérias de cada acesso separados e também unidos. Os acessos diferem quanto aos grupos de rizóbios capazes de formar simbiose.Submitted by Mario BC (mario@bc.ufrpe.br) on 2017-05-22T13:26:07Z No. of bitstreams: 1 Antunes Romeu Lima do Nascimento.pdf: 1176079 bytes, checksum: 3965be55696a10d228bab8c7064bc386 (MD5)Made available in DSpace on 2017-05-22T13:26:07Z (GMT). No. of bitstreams: 1 Antunes Romeu Lima do Nascimento.pdf: 1176079 bytes, checksum: 3965be55696a10d228bab8c7064bc386 (MD5) Previous issue date: 2014-02-24Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em Produção VegetalUFRPEBrasilUnidade Acadêmica de Serra TalhadaFeijão-favaPhaseolus lunatusRizóbioMorfofisiologiaFITOTECNIA::MELHORAMENTO VEGETALDiversidade e caracterização de rizóbios associados ao feijão-fava no semiáridoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-1747323715939935130600600600600-696890503755266288426156072994701319672075167498588264571info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPELICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/6937/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51ORIGINALAntunes Romeu Lima do Nascimento.pdfAntunes Romeu Lima do Nascimento.pdfapplication/pdf1176079http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/6937/2/Antunes+Romeu+Lima+do+Nascimento.pdf3965be55696a10d228bab8c7064bc386MD52tede2/69372018-08-30 12:37:04.366oai:tede2: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Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2024-05-28T12:34:59.938711Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false
dc.title.por.fl_str_mv Diversidade e caracterização de rizóbios associados ao feijão-fava no semiárido
title Diversidade e caracterização de rizóbios associados ao feijão-fava no semiárido
spellingShingle Diversidade e caracterização de rizóbios associados ao feijão-fava no semiárido
NASCIMENTO, Antunes Romeu Lima do
Feijão-fava
Phaseolus lunatus
Rizóbio
Morfofisiologia
FITOTECNIA::MELHORAMENTO VEGETAL
title_short Diversidade e caracterização de rizóbios associados ao feijão-fava no semiárido
title_full Diversidade e caracterização de rizóbios associados ao feijão-fava no semiárido
title_fullStr Diversidade e caracterização de rizóbios associados ao feijão-fava no semiárido
title_full_unstemmed Diversidade e caracterização de rizóbios associados ao feijão-fava no semiárido
title_sort Diversidade e caracterização de rizóbios associados ao feijão-fava no semiárido
author NASCIMENTO, Antunes Romeu Lima do
author_facet NASCIMENTO, Antunes Romeu Lima do
author_role author
dc.contributor.advisor1.fl_str_mv SANTOS, Carolina Etienne de Rosália e Silva
dc.contributor.advisor-co1.fl_str_mv FREITAS, Ana Dolores Santiago de
dc.contributor.referee1.fl_str_mv COSTA, Cynthia Maria Carneiro
dc.contributor.referee2.fl_str_mv PRAGANA, Rossana Barbosa
dc.contributor.referee3.fl_str_mv SILVA, Maria de Fátima da
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/9892727812146615
dc.contributor.author.fl_str_mv NASCIMENTO, Antunes Romeu Lima do
contributor_str_mv SANTOS, Carolina Etienne de Rosália e Silva
FREITAS, Ana Dolores Santiago de
COSTA, Cynthia Maria Carneiro
PRAGANA, Rossana Barbosa
SILVA, Maria de Fátima da
dc.subject.por.fl_str_mv Feijão-fava
Phaseolus lunatus
Rizóbio
Morfofisiologia
topic Feijão-fava
Phaseolus lunatus
Rizóbio
Morfofisiologia
FITOTECNIA::MELHORAMENTO VEGETAL
dc.subject.cnpq.fl_str_mv FITOTECNIA::MELHORAMENTO VEGETAL
description The lima bean is a legume producer of edible grains with high protein value, cultivated worldwide, having in Brazil, the Northeast region as the main producer. In this region, the production is made with low use of technology, resulting in low yields. Among the technologies used to increase the production of legume, we can highlight the inoculation of seed with diazotrophic bacterias, with the objective of replace or reduce nitrogen fertilization. The aim of this study was to evaluate the diversity of rhizobia in symbiosis with the lima bean adapted to semiarid conditions. To obtain the isolated was used 02 accessions of lima bean (one of indeterminate growth and other of determinate growth) and 12 composite samples of soil from different mesoregions the states of Paraíba and Pernambuco. Plants were cultived in a protected area with transparent canvas, in design completely randomized in factorial of 12 (soil) x two (accessions) in Leonard pots adapted with plastic bottle containing 1.3 L of sand and 100 mL soil, with three replicates, for 55 days. The variables analyzed were: number of nodules (NN), fresh mass of nodules (MFN), specifies biomass of nodules (BEN), dry mass of the part aerial (MSPA) and dry mass of root (MSR). Data were analyzed using the F test and the means compared by the Scott- Knott test. Of the collected nodules were isolated bacteria and determined their morphological and physiological characteristics in culture medium, in the laboratory of Soil Microbiology of UFRPE (Recife-PE). With the characteristics of the isolates was calculated the coefficient J and was performed the grouping of the bacteria. The soil of Alagoa Grande-PB showed the highest values for NN and MFN, however when analyzing the specific biomass of nodules no detectable differences. For MSR obtained only difference between accessions. And for MSPA differences were observed for both factors, emphasizing the soil of Alagoa Grande-PB, and two soils of native vegetation, Areia-PB and Garanhuns-PE, these being a possible source of efficient strains. The grouping of the bacteria showed that accession of growth determined formed 13 groups, while the other accession, 24. And analyzing all bacteria in one dendrogram were formed groups with bacteria of each separate accession and also united. Accessions differ in groups of rhizobia able to form symbiosis.
publishDate 2014
dc.date.issued.fl_str_mv 2014-02-24
dc.date.accessioned.fl_str_mv 2017-05-22T13:26:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv NASCIMENTO, Antunes Romeu Lima do. Diversidade e caracterização de rizóbios associados ao feijão-fava no semiárido. 2014. 53 f. Dissertação (Programa de Pós-Graduação em Produção Vegetal) - Universidade Federal Rural de Pernambuco, Serra Talhada.
dc.identifier.uri.fl_str_mv http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6937
identifier_str_mv NASCIMENTO, Antunes Romeu Lima do. Diversidade e caracterização de rizóbios associados ao feijão-fava no semiárido. 2014. 53 f. Dissertação (Programa de Pós-Graduação em Produção Vegetal) - Universidade Federal Rural de Pernambuco, Serra Talhada.
url http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6937
dc.language.iso.fl_str_mv por
language por
dc.relation.program.fl_str_mv -1747323715939935130
dc.relation.confidence.fl_str_mv 600
600
600
600
dc.relation.department.fl_str_mv -6968905037552662884
dc.relation.cnpq.fl_str_mv 2615607299470131967
dc.relation.sponsorship.fl_str_mv 2075167498588264571
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal Rural de Pernambuco
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Produção Vegetal
dc.publisher.initials.fl_str_mv UFRPE
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Unidade Acadêmica de Serra Talhada
publisher.none.fl_str_mv Universidade Federal Rural de Pernambuco
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da UFRPE
instname:Universidade Federal Rural de Pernambuco (UFRPE)
instacron:UFRPE
instname_str Universidade Federal Rural de Pernambuco (UFRPE)
instacron_str UFRPE
institution UFRPE
reponame_str Biblioteca Digital de Teses e Dissertações da UFRPE
collection Biblioteca Digital de Teses e Dissertações da UFRPE
bitstream.url.fl_str_mv http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/6937/1/license.txt
http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/6937/2/Antunes+Romeu+Lima+do+Nascimento.pdf
bitstream.checksum.fl_str_mv bd3efa91386c1718a7f26a329fdcb468
3965be55696a10d228bab8c7064bc386
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)
repository.mail.fl_str_mv bdtd@ufrpe.br ||bdtd@ufrpe.br
_version_ 1810102243800645632