Plasma rico em plaquetas: obtenção, momento de produção e uso na integração de flapes cutâneos de avanço em cães

Detalhes bibliográficos
Autor(a) principal: CAVALCANTI, Grazielle Anahy de Sousa Aleixo
Data de Publicação: 2010
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRPE
Texto Completo: http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5644
Resumo: A lot of emphasis has been given to the use of platelet rich plasma (PRP) in various areas of medicine, including reconstructive surgery, because when it is applied on a tissue in repair its capable of stimulating many important cellular processes, such as proliferation,differentiation, chemotaxis and angiogenesis. The aim of this paper was to evaluate the PRP effects over integration of advance skin flaps in dogs. In the first stage of the research, blood samples were collected from 20 dogs to compare two protocols used to obtain PRP. In the first protocol (A), blood sample was submitted to a single spin at 1200 revolutions per minute (rpm) for 10 minutes while in the second protocol (B), the sample was processed through two centrifugations, the first at 1200 rpm for 10 minutes and the second at 1600 rpm for 10 minutes. The results showed that protocol B was able to increase platelet count in 449,02% compared to whole blood, being higher than protocol A (190,22%). In the sequence, protocol B was applied in other 20 animals to evaluate its efficiency and it was found that the methodwas reproducible. After that, blood samples from 20 dogs were collected for production of PRP at two different moments: M0, corresponding to preoperative time, before anesthesia and fluid therapy, and M1, during trans-surgical period, after anesthesia. Platelets counts of blood collected in M0 were 28,58% higher than in M1, leading consequently, to the production of a more concentrated PRP. After defining the best protocol (B) and the ideal moment to collect the blood sample (M0) began the final stage of the experiment, in which two advance flaps were created in the ventral abdomen of eight dogs. Between one flap and recipient bed, PRP gel activated with thromboplastin was applied, whereas under the other flap was not applied any product(control). The flaps were clinically evaluated 24 hours after surgery (D1) and at every two days (D3, D5 and D7) until the seventh day after surgery. With ten days, both flaps were removed for histopathological examination and morphometry of blood vessels where it was demonstrated no significant difference between treated and control flap. Based on the results, it is concluded that platelet gel activated with thromboplastin did not enhance integration of advance skin flaps in dogs.
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spelling COELHO, Maria Cristina de Oliveira CardosoTEIXEIRA, Miriam NogueiraSILVA, José Antônio Aleixo daTUDURY , Eduardo AlbertoRODRIGUES, Marcelo CamposMENEZES, Flávia Ferreira dehttp://lattes.cnpq.br/3165940085830406CAVALCANTI, Grazielle Anahy de Sousa Aleixo2016-10-10T15:50:19Z2010-02-26CAVALCANTI, Grazielle Anahy de Sousa Aleixo. Plasma rico em plaquetas: obtenção, momento de produção e uso na integração de flapes cutâneos de avanço em cães. 2010. 131 f. Tese (Programa de Pós-Graduação em Ciência Veterinária) - Universidade Federal Rural de Pernambuco, Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5644A lot of emphasis has been given to the use of platelet rich plasma (PRP) in various areas of medicine, including reconstructive surgery, because when it is applied on a tissue in repair its capable of stimulating many important cellular processes, such as proliferation,differentiation, chemotaxis and angiogenesis. The aim of this paper was to evaluate the PRP effects over integration of advance skin flaps in dogs. In the first stage of the research, blood samples were collected from 20 dogs to compare two protocols used to obtain PRP. In the first protocol (A), blood sample was submitted to a single spin at 1200 revolutions per minute (rpm) for 10 minutes while in the second protocol (B), the sample was processed through two centrifugations, the first at 1200 rpm for 10 minutes and the second at 1600 rpm for 10 minutes. The results showed that protocol B was able to increase platelet count in 449,02% compared to whole blood, being higher than protocol A (190,22%). In the sequence, protocol B was applied in other 20 animals to evaluate its efficiency and it was found that the methodwas reproducible. After that, blood samples from 20 dogs were collected for production of PRP at two different moments: M0, corresponding to preoperative time, before anesthesia and fluid therapy, and M1, during trans-surgical period, after anesthesia. Platelets counts of blood collected in M0 were 28,58% higher than in M1, leading consequently, to the production of a more concentrated PRP. After defining the best protocol (B) and the ideal moment to collect the blood sample (M0) began the final stage of the experiment, in which two advance flaps were created in the ventral abdomen of eight dogs. Between one flap and recipient bed, PRP gel activated with thromboplastin was applied, whereas under the other flap was not applied any product(control). The flaps were clinically evaluated 24 hours after surgery (D1) and at every two days (D3, D5 and D7) until the seventh day after surgery. With ten days, both flaps were removed for histopathological examination and morphometry of blood vessels where it was demonstrated no significant difference between treated and control flap. Based on the results, it is concluded that platelet gel activated with thromboplastin did not enhance integration of advance skin flaps in dogs.Atualmente tem se dado muita ênfase ao uso do plasma rico em plaquetas (PRP) em diversas áreas da Medicina, inclusive em cirurgia reconstrutiva, pois ao ser aplicado sobre um tecido em reparação ele é capaz de estimular muitos processos celulares importantes, como a proliferação, diferenciação, quimiotaxia e angiogênese. Com este trabalho, objetivou-se avaliar a ação do PRP na integração de flapes cutâneos de avanço em animais da espécie canina. Na primeira etapa do experimento foram coletados amostras de sangue de 20 cães para comparar dois protocolos de obtenção do PRP. No primeiro (A), a amostra de sangue foi submetida a uma única centrifugação a 1200 rotações por minuto (rpm) durante 10 minutos enquanto que no segundo (B), a amostra foi processada através de duas centrifugações, sendo a primeira a 1200 rpm durante 10 minutos e a segunda em 1600 rpm durante 10 minutos. Os resultados demonstraram que o protocolo B foi capaz de incrementar a concentração plaquetária em 449,02% com relação ao sangue total, sendo este superior ao protocolo A (190,22%). Posteriomente se testou o protocolo B em outros 20 animais e foi constatado que ométodo em questão era reprodutível e eficiente. Na etapa seguinte, amostras de sangue de mais 20 cães foram utilizadas para produção do PRP em dois momentos distintos, sendo o primeiro (M0) correspondente ao período pré-operatório antes da anestesia e fluidoterapia, e o segundo (M1) durante o trans-operatório após a obtenção do plano anestésico. A coleta em M0 resultou em uma plaquetometria 28,58% mais alta do que em M1, culminando consequentemente, com a produção de um PRP mais concentrado. Definido o protocolo (B) e o momento ideal para a coleta da amostra de sangue (M0), seguiu-se para a etapa final do experimento, no qual foram criados dois flapes de avanço na região ventral do abdomen de oito cadelas. Entre um flape e o leito receptor, aplicou-se o gel de PRP ativado com tromboplastina, enquanto que no outro flap não foi aplicado nenhum produto (controle). Estes foram clinicamente avaliados 24 horas após a cirurgia (D1) e a cada dois dias (D3, D5 e D7) atéo sétimo dia de pós-operatório. Além disso, com dez dias ambos os flapes foram removidos para realização do exame histopatológico e morfometria dos vasos sanguíneos. Os achados demonstraram não haver diferença significativa entre o flape tratado e controle, concluindo-se que o gel de plaquetas ativado com tromboplastina não favoreceu a integração de flapes cutâneos de avanço em animais da espécie canina.Submitted by (edna.saturno@ufrpe.br) on 2016-10-10T15:50:19Z No. of bitstreams: 1 Grazielle Anahy de Sousa.pdf: 1926179 bytes, checksum: 8e81f0f99a362aeb986fac0cef28150c (MD5)Made available in DSpace on 2016-10-10T15:50:19Z (GMT). No. of bitstreams: 1 Grazielle Anahy de Sousa.pdf: 1926179 bytes, checksum: 8e81f0f99a362aeb986fac0cef28150c (MD5) Previous issue date: 2010-02-26Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqapplication/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em Ciência VeterináriaUFRPEBrasilDepartamento de Medicina VeterináriaCãoTrauma cutâneoFlapePlaquetaDogCutaneous traumaPlateletFlapCIENCIAS AGRARIAS::MEDICINA VETERINARIAPlasma rico em plaquetas: obtenção, momento de produção e uso na integração de flapes cutâneos de avanço em cãesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis-3061482854177903105600600600600-3020210563763616780453670264235017319-2555911436985713659info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPELICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/5644/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51ORIGINALGrazielle Anahy de Sousa.pdfGrazielle Anahy de Sousa.pdfapplication/pdf1926179http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/5644/2/Grazielle+Anahy+de+Sousa.pdf8e81f0f99a362aeb986fac0cef28150cMD52tede2/56442016-10-10 12:50:19.159oai:tede2: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Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2024-05-28T12:33:19.888405Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false
dc.title.por.fl_str_mv Plasma rico em plaquetas: obtenção, momento de produção e uso na integração de flapes cutâneos de avanço em cães
title Plasma rico em plaquetas: obtenção, momento de produção e uso na integração de flapes cutâneos de avanço em cães
spellingShingle Plasma rico em plaquetas: obtenção, momento de produção e uso na integração de flapes cutâneos de avanço em cães
CAVALCANTI, Grazielle Anahy de Sousa Aleixo
Cão
Trauma cutâneo
Flape
Plaqueta
Dog
Cutaneous trauma
Platelet
Flap
CIENCIAS AGRARIAS::MEDICINA VETERINARIA
title_short Plasma rico em plaquetas: obtenção, momento de produção e uso na integração de flapes cutâneos de avanço em cães
title_full Plasma rico em plaquetas: obtenção, momento de produção e uso na integração de flapes cutâneos de avanço em cães
title_fullStr Plasma rico em plaquetas: obtenção, momento de produção e uso na integração de flapes cutâneos de avanço em cães
title_full_unstemmed Plasma rico em plaquetas: obtenção, momento de produção e uso na integração de flapes cutâneos de avanço em cães
title_sort Plasma rico em plaquetas: obtenção, momento de produção e uso na integração de flapes cutâneos de avanço em cães
author CAVALCANTI, Grazielle Anahy de Sousa Aleixo
author_facet CAVALCANTI, Grazielle Anahy de Sousa Aleixo
author_role author
dc.contributor.advisor1.fl_str_mv COELHO, Maria Cristina de Oliveira Cardoso
dc.contributor.advisor-co1.fl_str_mv TEIXEIRA, Miriam Nogueira
dc.contributor.advisor-co2.fl_str_mv SILVA, José Antônio Aleixo da
dc.contributor.referee1.fl_str_mv TUDURY , Eduardo Alberto
dc.contributor.referee2.fl_str_mv RODRIGUES, Marcelo Campos
dc.contributor.referee3.fl_str_mv MENEZES, Flávia Ferreira de
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/3165940085830406
dc.contributor.author.fl_str_mv CAVALCANTI, Grazielle Anahy de Sousa Aleixo
contributor_str_mv COELHO, Maria Cristina de Oliveira Cardoso
TEIXEIRA, Miriam Nogueira
SILVA, José Antônio Aleixo da
TUDURY , Eduardo Alberto
RODRIGUES, Marcelo Campos
MENEZES, Flávia Ferreira de
dc.subject.por.fl_str_mv Cão
Trauma cutâneo
Flape
Plaqueta
topic Cão
Trauma cutâneo
Flape
Plaqueta
Dog
Cutaneous trauma
Platelet
Flap
CIENCIAS AGRARIAS::MEDICINA VETERINARIA
dc.subject.eng.fl_str_mv Dog
Cutaneous trauma
Platelet
Flap
dc.subject.cnpq.fl_str_mv CIENCIAS AGRARIAS::MEDICINA VETERINARIA
description A lot of emphasis has been given to the use of platelet rich plasma (PRP) in various areas of medicine, including reconstructive surgery, because when it is applied on a tissue in repair its capable of stimulating many important cellular processes, such as proliferation,differentiation, chemotaxis and angiogenesis. The aim of this paper was to evaluate the PRP effects over integration of advance skin flaps in dogs. In the first stage of the research, blood samples were collected from 20 dogs to compare two protocols used to obtain PRP. In the first protocol (A), blood sample was submitted to a single spin at 1200 revolutions per minute (rpm) for 10 minutes while in the second protocol (B), the sample was processed through two centrifugations, the first at 1200 rpm for 10 minutes and the second at 1600 rpm for 10 minutes. The results showed that protocol B was able to increase platelet count in 449,02% compared to whole blood, being higher than protocol A (190,22%). In the sequence, protocol B was applied in other 20 animals to evaluate its efficiency and it was found that the methodwas reproducible. After that, blood samples from 20 dogs were collected for production of PRP at two different moments: M0, corresponding to preoperative time, before anesthesia and fluid therapy, and M1, during trans-surgical period, after anesthesia. Platelets counts of blood collected in M0 were 28,58% higher than in M1, leading consequently, to the production of a more concentrated PRP. After defining the best protocol (B) and the ideal moment to collect the blood sample (M0) began the final stage of the experiment, in which two advance flaps were created in the ventral abdomen of eight dogs. Between one flap and recipient bed, PRP gel activated with thromboplastin was applied, whereas under the other flap was not applied any product(control). The flaps were clinically evaluated 24 hours after surgery (D1) and at every two days (D3, D5 and D7) until the seventh day after surgery. With ten days, both flaps were removed for histopathological examination and morphometry of blood vessels where it was demonstrated no significant difference between treated and control flap. Based on the results, it is concluded that platelet gel activated with thromboplastin did not enhance integration of advance skin flaps in dogs.
publishDate 2010
dc.date.issued.fl_str_mv 2010-02-26
dc.date.accessioned.fl_str_mv 2016-10-10T15:50:19Z
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dc.identifier.citation.fl_str_mv CAVALCANTI, Grazielle Anahy de Sousa Aleixo. Plasma rico em plaquetas: obtenção, momento de produção e uso na integração de flapes cutâneos de avanço em cães. 2010. 131 f. Tese (Programa de Pós-Graduação em Ciência Veterinária) - Universidade Federal Rural de Pernambuco, Recife.
dc.identifier.uri.fl_str_mv http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5644
identifier_str_mv CAVALCANTI, Grazielle Anahy de Sousa Aleixo. Plasma rico em plaquetas: obtenção, momento de produção e uso na integração de flapes cutâneos de avanço em cães. 2010. 131 f. Tese (Programa de Pós-Graduação em Ciência Veterinária) - Universidade Federal Rural de Pernambuco, Recife.
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