Expression of the cell cycle regulation proteins p53 and p21WAF1 in different types of non-dysplastic leukoplakias

Detalhes bibliográficos
Autor(a) principal: Visioli, Fernanda
Data de Publicação: 2012
Outros Autores: Lauxen, Isabel Silva, Sant'Ana Filho, Manoel, Rados, Pantelis Varvaki
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: https://www.revistas.usp.br/jaos/article/view/46246
Resumo: OBJECTIVES: The aim of this study was to analyze the immunolabeling of two cell cycle protein regulators, p53 and p21WAF1, in non-dysplastic leukoplakias with different epithelial alterations: acanthosis, hyperkeratosis and acanthosis combined with hyperkeratosis, and compare them with dysplastic leukoplakias. MATERIAL AND METHODS: This was a prospective cohort study involving 36 patients with oral homogeneous leukoplakias. excisional biopsies were performed and the patients remain under clinical follow-up. The leukoplakias were divided into four groups: 6 acanthosis, 9 hyperkeratosis, 10 acanthosis combined with hyperkeratosis, and 11 epithelial dysplasias. Paraffin-embebeded sections were immunostained for p53 and p21WAF1. Five hundred cells from the basal layer and 500 from the parabasal layer were counted to determine the percentage of positive cells. A qualitative analysis was also carried out to determine the presence or absence of immunohistochemical staining in the intermediate and superficial layers. Groups were compared with ANOVA (p
id USP-17_176af46dd07aee418f5295d8b7fe619d
oai_identifier_str oai:revistas.usp.br:article/46246
network_acronym_str USP-17
network_name_str Journal of applied oral science (Online)
repository_id_str
spelling Expression of the cell cycle regulation proteins p53 and p21WAF1 in different types of non-dysplastic leukoplakiasLeukoplakiaOral cancerTumor suppressor protein p53Cyclin-cependent Kinase inhibitor p21OBJECTIVES: The aim of this study was to analyze the immunolabeling of two cell cycle protein regulators, p53 and p21WAF1, in non-dysplastic leukoplakias with different epithelial alterations: acanthosis, hyperkeratosis and acanthosis combined with hyperkeratosis, and compare them with dysplastic leukoplakias. MATERIAL AND METHODS: This was a prospective cohort study involving 36 patients with oral homogeneous leukoplakias. excisional biopsies were performed and the patients remain under clinical follow-up. The leukoplakias were divided into four groups: 6 acanthosis, 9 hyperkeratosis, 10 acanthosis combined with hyperkeratosis, and 11 epithelial dysplasias. Paraffin-embebeded sections were immunostained for p53 and p21WAF1. Five hundred cells from the basal layer and 500 from the parabasal layer were counted to determine the percentage of positive cells. A qualitative analysis was also carried out to determine the presence or absence of immunohistochemical staining in the intermediate and superficial layers. Groups were compared with ANOVA (pUniversidade de São Paulo. Faculdade de Odontologia de Bauru2012-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/4624610.1590/S1678-77572012000300013Journal of Applied Oral Science; Vol. 20 No. 3 (2012); 369-375Journal of Applied Oral Science; Vol. 20 Núm. 3 (2012); 369-375Journal of Applied Oral Science; v. 20 n. 3 (2012); 369-3751678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/46246/49868Copyright (c) 2012 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessVisioli, FernandaLauxen, Isabel SilvaSant'Ana Filho, ManoelRados, Pantelis Varvaki2012-10-18T19:37:55Zoai:revistas.usp.br:article/46246Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2012-10-18T19:37:55Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Expression of the cell cycle regulation proteins p53 and p21WAF1 in different types of non-dysplastic leukoplakias
title Expression of the cell cycle regulation proteins p53 and p21WAF1 in different types of non-dysplastic leukoplakias
spellingShingle Expression of the cell cycle regulation proteins p53 and p21WAF1 in different types of non-dysplastic leukoplakias
Visioli, Fernanda
Leukoplakia
Oral cancer
Tumor suppressor protein p53
Cyclin-cependent Kinase inhibitor p21
title_short Expression of the cell cycle regulation proteins p53 and p21WAF1 in different types of non-dysplastic leukoplakias
title_full Expression of the cell cycle regulation proteins p53 and p21WAF1 in different types of non-dysplastic leukoplakias
title_fullStr Expression of the cell cycle regulation proteins p53 and p21WAF1 in different types of non-dysplastic leukoplakias
title_full_unstemmed Expression of the cell cycle regulation proteins p53 and p21WAF1 in different types of non-dysplastic leukoplakias
title_sort Expression of the cell cycle regulation proteins p53 and p21WAF1 in different types of non-dysplastic leukoplakias
author Visioli, Fernanda
author_facet Visioli, Fernanda
Lauxen, Isabel Silva
Sant'Ana Filho, Manoel
Rados, Pantelis Varvaki
author_role author
author2 Lauxen, Isabel Silva
Sant'Ana Filho, Manoel
Rados, Pantelis Varvaki
author2_role author
author
author
dc.contributor.author.fl_str_mv Visioli, Fernanda
Lauxen, Isabel Silva
Sant'Ana Filho, Manoel
Rados, Pantelis Varvaki
dc.subject.por.fl_str_mv Leukoplakia
Oral cancer
Tumor suppressor protein p53
Cyclin-cependent Kinase inhibitor p21
topic Leukoplakia
Oral cancer
Tumor suppressor protein p53
Cyclin-cependent Kinase inhibitor p21
description OBJECTIVES: The aim of this study was to analyze the immunolabeling of two cell cycle protein regulators, p53 and p21WAF1, in non-dysplastic leukoplakias with different epithelial alterations: acanthosis, hyperkeratosis and acanthosis combined with hyperkeratosis, and compare them with dysplastic leukoplakias. MATERIAL AND METHODS: This was a prospective cohort study involving 36 patients with oral homogeneous leukoplakias. excisional biopsies were performed and the patients remain under clinical follow-up. The leukoplakias were divided into four groups: 6 acanthosis, 9 hyperkeratosis, 10 acanthosis combined with hyperkeratosis, and 11 epithelial dysplasias. Paraffin-embebeded sections were immunostained for p53 and p21WAF1. Five hundred cells from the basal layer and 500 from the parabasal layer were counted to determine the percentage of positive cells. A qualitative analysis was also carried out to determine the presence or absence of immunohistochemical staining in the intermediate and superficial layers. Groups were compared with ANOVA (p
publishDate 2012
dc.date.none.fl_str_mv 2012-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/jaos/article/view/46246
10.1590/S1678-77572012000300013
url https://www.revistas.usp.br/jaos/article/view/46246
identifier_str_mv 10.1590/S1678-77572012000300013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/jaos/article/view/46246/49868
dc.rights.driver.fl_str_mv Copyright (c) 2012 Journal of Applied Oral Science
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2012 Journal of Applied Oral Science
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
dc.source.none.fl_str_mv Journal of Applied Oral Science; Vol. 20 No. 3 (2012); 369-375
Journal of Applied Oral Science; Vol. 20 Núm. 3 (2012); 369-375
Journal of Applied Oral Science; v. 20 n. 3 (2012); 369-375
1678-7765
1678-7757
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
_version_ 1800221677440729088

Registros relacionados