Periodontal disease severity is associated to pathogenic consortia comprising putative and candidate periodontal pathogens
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/206654 |
Resumo: | Based on a holistic concept of polymicrobial etiology, we have hypothesized that putative and candidate periodontal pathogens are more frequently detected in consortia than alone in advanced forms of periodontal diseases (PD). Objective: To correlate specific consortia of periodontal pathogens with clinical periodontal status and severity of periodontitis. Methodology: Subgingival biofilm was obtained from individuals with periodontal health (113, PH), gingivitis (91, G), and periodontitis (209, P). Genomic DNA was purified and the species Aggregatibacter actinomycetemcomitans (Aa), Aa JP2-like strain, Porphyromonas gingivalis (Pg), Dialister pneumosintes (Dp), and Filifactor alocis (Fa) were detected by PCR. Configural frequency and logistic regression analyses were performed to correlate microbial consortia and PD. Results: Aa + Pg in the presence of Dp (phi=0.240; χ2=11.9, p<0.01), as well as Aa JP2 + Dp + Fa (phi=0.186, χ2=4.6, p<0.05) were significantly more associated in advanced stages of P. The consortium Aa + Fa + Dp was strongly associated with deep pocketing and inflammation (p<0.001). The best predictors of disease severity (80% accuracy) included older age (OR 1.11 [95% CI 1.07 – 1.15], p<0.001), Black/African-American ancestry (OR 1.89 [95% CI 1.19 – 2.99], p=0.007), and high frequency of Aa + Pg + Dp (OR 3.04 [95% CI 1.49 – 6.22], p=0.002). Conclusion; Specific microbial consortia of putative and novel periodontal pathogens, associated with demographic parameters, correlate with severe periodontitis, supporting the multifactorial nature of PD. |
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Journal of applied oral science (Online) |
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Periodontal disease severity is associated to pathogenic consortia comprising putative and candidate periodontal pathogensGingivitisPeriodontitisDental plaquePCRMicrobial consortiaBased on a holistic concept of polymicrobial etiology, we have hypothesized that putative and candidate periodontal pathogens are more frequently detected in consortia than alone in advanced forms of periodontal diseases (PD). Objective: To correlate specific consortia of periodontal pathogens with clinical periodontal status and severity of periodontitis. Methodology: Subgingival biofilm was obtained from individuals with periodontal health (113, PH), gingivitis (91, G), and periodontitis (209, P). Genomic DNA was purified and the species Aggregatibacter actinomycetemcomitans (Aa), Aa JP2-like strain, Porphyromonas gingivalis (Pg), Dialister pneumosintes (Dp), and Filifactor alocis (Fa) were detected by PCR. Configural frequency and logistic regression analyses were performed to correlate microbial consortia and PD. Results: Aa + Pg in the presence of Dp (phi=0.240; χ2=11.9, p<0.01), as well as Aa JP2 + Dp + Fa (phi=0.186, χ2=4.6, p<0.05) were significantly more associated in advanced stages of P. The consortium Aa + Fa + Dp was strongly associated with deep pocketing and inflammation (p<0.001). The best predictors of disease severity (80% accuracy) included older age (OR 1.11 [95% CI 1.07 – 1.15], p<0.001), Black/African-American ancestry (OR 1.89 [95% CI 1.19 – 2.99], p=0.007), and high frequency of Aa + Pg + Dp (OR 3.04 [95% CI 1.49 – 6.22], p=0.002). Conclusion; Specific microbial consortia of putative and novel periodontal pathogens, associated with demographic parameters, correlate with severe periodontitis, supporting the multifactorial nature of PD.Universidade de São Paulo. Faculdade de Odontologia de Bauru2023-01-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/20665410.1590/1678-7757-2022-0359Journal of Applied Oral Science; Vol. 31 (2023); e20220359Journal of Applied Oral Science; Vol. 31 (2023); e20220359Journal of Applied Oral Science; v. 31 (2023); e202203591678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/206654/190366Copyright (c) 2023 Journal of Applied Oral Sciencehttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessAraújo, Lélia Lima Lourenço, Talita Gomes BaêtaColombo, Ana Paula Vieira 2023-01-10T18:32:08Zoai:revistas.usp.br:article/206654Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2023-01-10T18:32:08Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Periodontal disease severity is associated to pathogenic consortia comprising putative and candidate periodontal pathogens |
title |
Periodontal disease severity is associated to pathogenic consortia comprising putative and candidate periodontal pathogens |
spellingShingle |
Periodontal disease severity is associated to pathogenic consortia comprising putative and candidate periodontal pathogens Araújo, Lélia Lima Gingivitis Periodontitis Dental plaque PCR Microbial consortia |
title_short |
Periodontal disease severity is associated to pathogenic consortia comprising putative and candidate periodontal pathogens |
title_full |
Periodontal disease severity is associated to pathogenic consortia comprising putative and candidate periodontal pathogens |
title_fullStr |
Periodontal disease severity is associated to pathogenic consortia comprising putative and candidate periodontal pathogens |
title_full_unstemmed |
Periodontal disease severity is associated to pathogenic consortia comprising putative and candidate periodontal pathogens |
title_sort |
Periodontal disease severity is associated to pathogenic consortia comprising putative and candidate periodontal pathogens |
author |
Araújo, Lélia Lima |
author_facet |
Araújo, Lélia Lima Lourenço, Talita Gomes Baêta Colombo, Ana Paula Vieira |
author_role |
author |
author2 |
Lourenço, Talita Gomes Baêta Colombo, Ana Paula Vieira |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Araújo, Lélia Lima Lourenço, Talita Gomes Baêta Colombo, Ana Paula Vieira |
dc.subject.por.fl_str_mv |
Gingivitis Periodontitis Dental plaque PCR Microbial consortia |
topic |
Gingivitis Periodontitis Dental plaque PCR Microbial consortia |
description |
Based on a holistic concept of polymicrobial etiology, we have hypothesized that putative and candidate periodontal pathogens are more frequently detected in consortia than alone in advanced forms of periodontal diseases (PD). Objective: To correlate specific consortia of periodontal pathogens with clinical periodontal status and severity of periodontitis. Methodology: Subgingival biofilm was obtained from individuals with periodontal health (113, PH), gingivitis (91, G), and periodontitis (209, P). Genomic DNA was purified and the species Aggregatibacter actinomycetemcomitans (Aa), Aa JP2-like strain, Porphyromonas gingivalis (Pg), Dialister pneumosintes (Dp), and Filifactor alocis (Fa) were detected by PCR. Configural frequency and logistic regression analyses were performed to correlate microbial consortia and PD. Results: Aa + Pg in the presence of Dp (phi=0.240; χ2=11.9, p<0.01), as well as Aa JP2 + Dp + Fa (phi=0.186, χ2=4.6, p<0.05) were significantly more associated in advanced stages of P. The consortium Aa + Fa + Dp was strongly associated with deep pocketing and inflammation (p<0.001). The best predictors of disease severity (80% accuracy) included older age (OR 1.11 [95% CI 1.07 – 1.15], p<0.001), Black/African-American ancestry (OR 1.89 [95% CI 1.19 – 2.99], p=0.007), and high frequency of Aa + Pg + Dp (OR 3.04 [95% CI 1.49 – 6.22], p=0.002). Conclusion; Specific microbial consortia of putative and novel periodontal pathogens, associated with demographic parameters, correlate with severe periodontitis, supporting the multifactorial nature of PD. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-01-10 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/206654 10.1590/1678-7757-2022-0359 |
url |
https://www.revistas.usp.br/jaos/article/view/206654 |
identifier_str_mv |
10.1590/1678-7757-2022-0359 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/206654/190366 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2023 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2023 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 31 (2023); e20220359 Journal of Applied Oral Science; Vol. 31 (2023); e20220359 Journal of Applied Oral Science; v. 31 (2023); e20220359 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221683316948992 |