Catechin-incorporated dental copolymers inhibit growth of Streptococcus mutans

Detalhes bibliográficos
Autor(a) principal: Mankovskaia, Alexandra
Data de Publicação: 2013
Outros Autores: Levesque, Celine M., Prakki, Anuradha
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: https://www.revistas.usp.br/jaos/article/view/80456
Resumo: Objective: To test the inhibitory growth activity of green tea catechin incorporated into dental resins compared to resins containing the broad-spectrum antimicrobial compound chlorhexidine against Streptococcus mutans in vitro. Material and Methods: The minimum inhibitory concentrations (MICs) of epigallocatechin-gallate (EGCg) and chlorhexidine (CHX) were determined according to the microdilution method. Resin discs (5 mm × 3 mm) were prepared from Bis-GMA/TEGDMA (R1) and Bis-GMA/CH3Bis-GMA (R2) comonomers (n=9) containing: a) no drug, b) EGCg, c) CHX. Two concentrations of each drug (0.5× MIC and 1× MIC) were incorporated into the resin discs. Samples were individually immersed in a bacterial culture and incubated for 24 h at 37°C under constant agitation. Cell viability was assessed by counting the number of colonies on replica agar plates. Statistical analysis was performed using one-way ANOVA, Tukey and Student t-tests (α=0.05). Results: Both resins containing EGCg and CHX showed a significant inhibition of bacterial growth at both concentrations tested (p
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spelling Catechin-incorporated dental copolymers inhibit growth of Streptococcus mutans Objective: To test the inhibitory growth activity of green tea catechin incorporated into dental resins compared to resins containing the broad-spectrum antimicrobial compound chlorhexidine against Streptococcus mutans in vitro. Material and Methods: The minimum inhibitory concentrations (MICs) of epigallocatechin-gallate (EGCg) and chlorhexidine (CHX) were determined according to the microdilution method. Resin discs (5 mm × 3 mm) were prepared from Bis-GMA/TEGDMA (R1) and Bis-GMA/CH3Bis-GMA (R2) comonomers (n=9) containing: a) no drug, b) EGCg, c) CHX. Two concentrations of each drug (0.5× MIC and 1× MIC) were incorporated into the resin discs. Samples were individually immersed in a bacterial culture and incubated for 24 h at 37°C under constant agitation. Cell viability was assessed by counting the number of colonies on replica agar plates. Statistical analysis was performed using one-way ANOVA, Tukey and Student t-tests (α=0.05). Results: Both resins containing EGCg and CHX showed a significant inhibition of bacterial growth at both concentrations tested (pUniversidade de São Paulo. Faculdade de Odontologia de Bauru2013-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/8045610.1590/1678-7757201302430Journal of Applied Oral Science; Vol. 21 No. 2 (2013); 203-207Journal of Applied Oral Science; Vol. 21 Núm. 2 (2013); 203-207Journal of Applied Oral Science; v. 21 n. 2 (2013); 203-2071678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/80456/84119Copyright (c) 2013 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessMankovskaia, Alexandra Levesque, Celine M. Prakki, Anuradha 2014-05-08T12:31:56Zoai:revistas.usp.br:article/80456Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2014-05-08T12:31:56Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Catechin-incorporated dental copolymers inhibit growth of Streptococcus mutans
title Catechin-incorporated dental copolymers inhibit growth of Streptococcus mutans
spellingShingle Catechin-incorporated dental copolymers inhibit growth of Streptococcus mutans
Mankovskaia, Alexandra
title_short Catechin-incorporated dental copolymers inhibit growth of Streptococcus mutans
title_full Catechin-incorporated dental copolymers inhibit growth of Streptococcus mutans
title_fullStr Catechin-incorporated dental copolymers inhibit growth of Streptococcus mutans
title_full_unstemmed Catechin-incorporated dental copolymers inhibit growth of Streptococcus mutans
title_sort Catechin-incorporated dental copolymers inhibit growth of Streptococcus mutans
author Mankovskaia, Alexandra
author_facet Mankovskaia, Alexandra
Levesque, Celine M.
Prakki, Anuradha
author_role author
author2 Levesque, Celine M.
Prakki, Anuradha
author2_role author
author
dc.contributor.author.fl_str_mv Mankovskaia, Alexandra
Levesque, Celine M.
Prakki, Anuradha
description Objective: To test the inhibitory growth activity of green tea catechin incorporated into dental resins compared to resins containing the broad-spectrum antimicrobial compound chlorhexidine against Streptococcus mutans in vitro. Material and Methods: The minimum inhibitory concentrations (MICs) of epigallocatechin-gallate (EGCg) and chlorhexidine (CHX) were determined according to the microdilution method. Resin discs (5 mm × 3 mm) were prepared from Bis-GMA/TEGDMA (R1) and Bis-GMA/CH3Bis-GMA (R2) comonomers (n=9) containing: a) no drug, b) EGCg, c) CHX. Two concentrations of each drug (0.5× MIC and 1× MIC) were incorporated into the resin discs. Samples were individually immersed in a bacterial culture and incubated for 24 h at 37°C under constant agitation. Cell viability was assessed by counting the number of colonies on replica agar plates. Statistical analysis was performed using one-way ANOVA, Tukey and Student t-tests (α=0.05). Results: Both resins containing EGCg and CHX showed a significant inhibition of bacterial growth at both concentrations tested (p
publishDate 2013
dc.date.none.fl_str_mv 2013-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/jaos/article/view/80456
10.1590/1678-7757201302430
url https://www.revistas.usp.br/jaos/article/view/80456
identifier_str_mv 10.1590/1678-7757201302430
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/jaos/article/view/80456/84119
dc.rights.driver.fl_str_mv Copyright (c) 2013 Journal of Applied Oral Science
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2013 Journal of Applied Oral Science
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
dc.source.none.fl_str_mv Journal of Applied Oral Science; Vol. 21 No. 2 (2013); 203-207
Journal of Applied Oral Science; Vol. 21 Núm. 2 (2013); 203-207
Journal of Applied Oral Science; v. 21 n. 2 (2013); 203-207
1678-7765
1678-7757
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
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