Fcγ receptors on aging neutrophils

Detalhes bibliográficos
Autor(a) principal: Gasparoto, Thaís Helena
Data de Publicação: 2021
Outros Autores: Dalboni, Thalita Marcato, Amôr, Nádia Ghinelli, Abe, Aneli Eiko, Perri, Graziela, Lara, Vanessa Soares, Vieira, Narciso Almeida, Gasparoto, Carlos Teodoro, Campanelli, Ana Paula
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: https://www.revistas.usp.br/jaos/article/view/187217
Resumo: Objective: Neutrophils are key effector cells of the innate immune system. They recognize antigens through membrane receptors, which are expressed during their maturation and activation. Neutrophils express FcγRII (CD32), FcγRIII (CD16), and FcγRI (CD64) after being activated by different factors such as cytokines and bacterial products. These receptors are involved with phagocytosis of IgG-opsonized microbes and enhance defense mechanisms. Based on that, our study seeks to compare the expression of FcγRII, FcγRIII, FcγRI, and CD11b on neutrophils from elderly and young subjects and their expression after in vitro activation with cytokines and LPS. Methodology: Neutrophils were isolated from human peripheral blood and from mice bone marrow by density gradient. After isolation, FCγRs expression was immediately analyzed by flow cytometry or after in vitro stimulation. Results: In freshly isolated cells, the percentage of FcγRIIIb+ and CD11b+ neutrophils were higher in samples from young individuals; FcγRIIIa expression was more prominent on aged neutrophils; FcγRIA expression was similar in all samples analyzed. Exposure to CXCL8 and LPS resulted in a higher percentage of FcγRIa+ neutrophils on elderly individuals’ samples but lower when compared with neutrophils from young donors. We observed that LPS caused an increase in FcγRIIa expression on aging human neutrophils. In contrast, FcγRIIIb expression in response to CXCL8 and LPS stimulation was not altered in the four groups. CD11b expression was lower in neutrophils from elderly individuals even in response to LPS and CXCL8. In mice, we observed differences only regarding CD11b expression, which was increased on aged neutrophils. LPS exposure caused an increase in all FcγRs. Conclusions: Our results suggest that, in humans, the overall pattern of FcγR expression and integrin CD11b are altered during aging and immunosenescence might contribute to age-related infection.
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spelling Fcγ receptors on aging neutrophilsReceptorsIgGNeutrophilsAgingObjective: Neutrophils are key effector cells of the innate immune system. They recognize antigens through membrane receptors, which are expressed during their maturation and activation. Neutrophils express FcγRII (CD32), FcγRIII (CD16), and FcγRI (CD64) after being activated by different factors such as cytokines and bacterial products. These receptors are involved with phagocytosis of IgG-opsonized microbes and enhance defense mechanisms. Based on that, our study seeks to compare the expression of FcγRII, FcγRIII, FcγRI, and CD11b on neutrophils from elderly and young subjects and their expression after in vitro activation with cytokines and LPS. Methodology: Neutrophils were isolated from human peripheral blood and from mice bone marrow by density gradient. After isolation, FCγRs expression was immediately analyzed by flow cytometry or after in vitro stimulation. Results: In freshly isolated cells, the percentage of FcγRIIIb+ and CD11b+ neutrophils were higher in samples from young individuals; FcγRIIIa expression was more prominent on aged neutrophils; FcγRIA expression was similar in all samples analyzed. Exposure to CXCL8 and LPS resulted in a higher percentage of FcγRIa+ neutrophils on elderly individuals’ samples but lower when compared with neutrophils from young donors. We observed that LPS caused an increase in FcγRIIa expression on aging human neutrophils. In contrast, FcγRIIIb expression in response to CXCL8 and LPS stimulation was not altered in the four groups. CD11b expression was lower in neutrophils from elderly individuals even in response to LPS and CXCL8. In mice, we observed differences only regarding CD11b expression, which was increased on aged neutrophils. LPS exposure caused an increase in all FcγRs. Conclusions: Our results suggest that, in humans, the overall pattern of FcγR expression and integrin CD11b are altered during aging and immunosenescence might contribute to age-related infection.Universidade de São Paulo. Faculdade de Odontologia de Bauru2021-06-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/18721710.1590/1678-7757-2020-0770Journal of Applied Oral Science; Vol. 29 (2021); e20200770Journal of Applied Oral Science; Vol. 29 (2021); e20200770Journal of Applied Oral Science; v. 29 (2021); e202007701678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/187217/173032Copyright (c) 2021 Journal of Applied Oral Sciencehttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessGasparoto, Thaís Helena Dalboni, Thalita Marcato Amôr, Nádia Ghinelli Abe, Aneli Eiko Perri, Graziela Lara, Vanessa Soares Vieira, Narciso Almeida Gasparoto, Carlos Teodoro Campanelli, Ana Paula 2021-06-14T22:40:45Zoai:revistas.usp.br:article/187217Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2021-06-14T22:40:45Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Fcγ receptors on aging neutrophils
title Fcγ receptors on aging neutrophils
spellingShingle Fcγ receptors on aging neutrophils
Gasparoto, Thaís Helena
Receptors
IgG
Neutrophils
Aging
title_short Fcγ receptors on aging neutrophils
title_full Fcγ receptors on aging neutrophils
title_fullStr Fcγ receptors on aging neutrophils
title_full_unstemmed Fcγ receptors on aging neutrophils
title_sort Fcγ receptors on aging neutrophils
author Gasparoto, Thaís Helena
author_facet Gasparoto, Thaís Helena
Dalboni, Thalita Marcato
Amôr, Nádia Ghinelli
Abe, Aneli Eiko
Perri, Graziela
Lara, Vanessa Soares
Vieira, Narciso Almeida
Gasparoto, Carlos Teodoro
Campanelli, Ana Paula
author_role author
author2 Dalboni, Thalita Marcato
Amôr, Nádia Ghinelli
Abe, Aneli Eiko
Perri, Graziela
Lara, Vanessa Soares
Vieira, Narciso Almeida
Gasparoto, Carlos Teodoro
Campanelli, Ana Paula
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Gasparoto, Thaís Helena
Dalboni, Thalita Marcato
Amôr, Nádia Ghinelli
Abe, Aneli Eiko
Perri, Graziela
Lara, Vanessa Soares
Vieira, Narciso Almeida
Gasparoto, Carlos Teodoro
Campanelli, Ana Paula
dc.subject.por.fl_str_mv Receptors
IgG
Neutrophils
Aging
topic Receptors
IgG
Neutrophils
Aging
description Objective: Neutrophils are key effector cells of the innate immune system. They recognize antigens through membrane receptors, which are expressed during their maturation and activation. Neutrophils express FcγRII (CD32), FcγRIII (CD16), and FcγRI (CD64) after being activated by different factors such as cytokines and bacterial products. These receptors are involved with phagocytosis of IgG-opsonized microbes and enhance defense mechanisms. Based on that, our study seeks to compare the expression of FcγRII, FcγRIII, FcγRI, and CD11b on neutrophils from elderly and young subjects and their expression after in vitro activation with cytokines and LPS. Methodology: Neutrophils were isolated from human peripheral blood and from mice bone marrow by density gradient. After isolation, FCγRs expression was immediately analyzed by flow cytometry or after in vitro stimulation. Results: In freshly isolated cells, the percentage of FcγRIIIb+ and CD11b+ neutrophils were higher in samples from young individuals; FcγRIIIa expression was more prominent on aged neutrophils; FcγRIA expression was similar in all samples analyzed. Exposure to CXCL8 and LPS resulted in a higher percentage of FcγRIa+ neutrophils on elderly individuals’ samples but lower when compared with neutrophils from young donors. We observed that LPS caused an increase in FcγRIIa expression on aging human neutrophils. In contrast, FcγRIIIb expression in response to CXCL8 and LPS stimulation was not altered in the four groups. CD11b expression was lower in neutrophils from elderly individuals even in response to LPS and CXCL8. In mice, we observed differences only regarding CD11b expression, which was increased on aged neutrophils. LPS exposure caused an increase in all FcγRs. Conclusions: Our results suggest that, in humans, the overall pattern of FcγR expression and integrin CD11b are altered during aging and immunosenescence might contribute to age-related infection.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-14
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/jaos/article/view/187217
10.1590/1678-7757-2020-0770
url https://www.revistas.usp.br/jaos/article/view/187217
identifier_str_mv 10.1590/1678-7757-2020-0770
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/jaos/article/view/187217/173032
dc.rights.driver.fl_str_mv Copyright (c) 2021 Journal of Applied Oral Science
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2021 Journal of Applied Oral Science
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
dc.source.none.fl_str_mv Journal of Applied Oral Science; Vol. 29 (2021); e20200770
Journal of Applied Oral Science; Vol. 29 (2021); e20200770
Journal of Applied Oral Science; v. 29 (2021); e20200770
1678-7765
1678-7757
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
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