Non-cross-linked collagen type I/III materials enhance cell proliferation: in vitro and in vivo evidence
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/80539 |
Resumo: | Objective: To analyze Mucograft®(MG), a recently introduced collagen matrix, in vitro and in vivo, and compare it with BioGide®(BG), a well-established collagen membrane, as control. Material and Methods: A detailed analysis of the materials surface and ultra-structure was performed. Cellular growth patterns and proliferation rates of human fibroblasts on MG and BG were analyzed in vitro. In addition, the early tissue reaction of CD-1 mouse to these materials was analyzed by means of histological and histomorphometrical analysis. Results: MG showed a three-fold higher thickness both in dry and wet conditions, when compared to BG. The spongy surface of BG significantly differed from that of MG. Cells showed a characteristic proliferation pattern on the different materials in vitro. Fibroblasts tended to proliferate on the compact layers of both collagens, with the highest values on the compact side of BG. In vivo, at day three both materials demonstrated good tissue integration, with a mononuclear cell sheet of fibroblasts on all surfaces, however, without penetrating into the materials. Conclusions: The findings of this study showed that MG and BG facilitate cell proliferation on both of their surfaces in vitro. In vivo, these two materials induce a comparable early tissue reaction, while serving as cell occlusive barriers. |
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Journal of applied oral science (Online) |
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Non-cross-linked collagen type I/III materials enhance cell proliferation: in vitro and in vivo evidence Objective: To analyze Mucograft®(MG), a recently introduced collagen matrix, in vitro and in vivo, and compare it with BioGide®(BG), a well-established collagen membrane, as control. Material and Methods: A detailed analysis of the materials surface and ultra-structure was performed. Cellular growth patterns and proliferation rates of human fibroblasts on MG and BG were analyzed in vitro. In addition, the early tissue reaction of CD-1 mouse to these materials was analyzed by means of histological and histomorphometrical analysis. Results: MG showed a three-fold higher thickness both in dry and wet conditions, when compared to BG. The spongy surface of BG significantly differed from that of MG. Cells showed a characteristic proliferation pattern on the different materials in vitro. Fibroblasts tended to proliferate on the compact layers of both collagens, with the highest values on the compact side of BG. In vivo, at day three both materials demonstrated good tissue integration, with a mononuclear cell sheet of fibroblasts on all surfaces, however, without penetrating into the materials. Conclusions: The findings of this study showed that MG and BG facilitate cell proliferation on both of their surfaces in vitro. In vivo, these two materials induce a comparable early tissue reaction, while serving as cell occlusive barriers. Universidade de São Paulo. Faculdade de Odontologia de Bauru2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/8053910.1590/1678-775720130316Journal of Applied Oral Science; Vol. 22 No. 1 (2014); 29-37Journal of Applied Oral Science; Vol. 22 Núm. 1 (2014); 29-37Journal of Applied Oral Science; v. 22 n. 1 (2014); 29-371678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/80539/84202Copyright (c) 2014 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessWILLERSHAUSEN, Ines BARBECK, Mike BOEHM, Nicole SADER, Robert WILLERSHAUSEN, Brita KIRKPATRICK, Charles James GHANAATI, Shahram 2014-05-08T13:28:51Zoai:revistas.usp.br:article/80539Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2014-05-08T13:28:51Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Non-cross-linked collagen type I/III materials enhance cell proliferation: in vitro and in vivo evidence |
title |
Non-cross-linked collagen type I/III materials enhance cell proliferation: in vitro and in vivo evidence |
spellingShingle |
Non-cross-linked collagen type I/III materials enhance cell proliferation: in vitro and in vivo evidence WILLERSHAUSEN, Ines |
title_short |
Non-cross-linked collagen type I/III materials enhance cell proliferation: in vitro and in vivo evidence |
title_full |
Non-cross-linked collagen type I/III materials enhance cell proliferation: in vitro and in vivo evidence |
title_fullStr |
Non-cross-linked collagen type I/III materials enhance cell proliferation: in vitro and in vivo evidence |
title_full_unstemmed |
Non-cross-linked collagen type I/III materials enhance cell proliferation: in vitro and in vivo evidence |
title_sort |
Non-cross-linked collagen type I/III materials enhance cell proliferation: in vitro and in vivo evidence |
author |
WILLERSHAUSEN, Ines |
author_facet |
WILLERSHAUSEN, Ines BARBECK, Mike BOEHM, Nicole SADER, Robert WILLERSHAUSEN, Brita KIRKPATRICK, Charles James GHANAATI, Shahram |
author_role |
author |
author2 |
BARBECK, Mike BOEHM, Nicole SADER, Robert WILLERSHAUSEN, Brita KIRKPATRICK, Charles James GHANAATI, Shahram |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
WILLERSHAUSEN, Ines BARBECK, Mike BOEHM, Nicole SADER, Robert WILLERSHAUSEN, Brita KIRKPATRICK, Charles James GHANAATI, Shahram |
description |
Objective: To analyze Mucograft®(MG), a recently introduced collagen matrix, in vitro and in vivo, and compare it with BioGide®(BG), a well-established collagen membrane, as control. Material and Methods: A detailed analysis of the materials surface and ultra-structure was performed. Cellular growth patterns and proliferation rates of human fibroblasts on MG and BG were analyzed in vitro. In addition, the early tissue reaction of CD-1 mouse to these materials was analyzed by means of histological and histomorphometrical analysis. Results: MG showed a three-fold higher thickness both in dry and wet conditions, when compared to BG. The spongy surface of BG significantly differed from that of MG. Cells showed a characteristic proliferation pattern on the different materials in vitro. Fibroblasts tended to proliferate on the compact layers of both collagens, with the highest values on the compact side of BG. In vivo, at day three both materials demonstrated good tissue integration, with a mononuclear cell sheet of fibroblasts on all surfaces, however, without penetrating into the materials. Conclusions: The findings of this study showed that MG and BG facilitate cell proliferation on both of their surfaces in vitro. In vivo, these two materials induce a comparable early tissue reaction, while serving as cell occlusive barriers. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/80539 10.1590/1678-775720130316 |
url |
https://www.revistas.usp.br/jaos/article/view/80539 |
identifier_str_mv |
10.1590/1678-775720130316 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/80539/84202 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2014 Journal of Applied Oral Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2014 Journal of Applied Oral Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 22 No. 1 (2014); 29-37 Journal of Applied Oral Science; Vol. 22 Núm. 1 (2014); 29-37 Journal of Applied Oral Science; v. 22 n. 1 (2014); 29-37 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221678102380544 |