Effect of fluoride gels with nano-sized sodium trimetaphosphate on the in vitro remineralization of caries lesions

Detalhes bibliográficos
Autor(a) principal: Nagata, Mariana Emi
Data de Publicação: 2023
Outros Autores: Delbem, Alberto Carlos Botazzo, Báez-Quintero, Liliana Carolina, Danelon, Marcelle, Sampaio, Caio, Monteiro, Douglas Roberto, Wiegand, Annette, Pessan, Juliano Pelim
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: https://www.revistas.usp.br/jaos/article/view/213811
Resumo: Objective: To evaluate the effects of fluoride (F) gels supplemented with micrometric or nano-sized sodium trimetaphosphate (TMPmicro and TMPnano, respectively) on the in vitro remineralization of caries-like lesions. Methodology: Bovine enamel subsurface lesions (n=168) were selected according to their surface hardness (SH) and randomly divided into seven groups (n=24/group): Placebo (without F/TMP), 4,500 ppm F (4500F), 4500F + 2.5% TMPnano (2.5% Nano), 4500F + 5% TMPnano (5% Nano), 4500F + 5% TMPmicro (5% Micro), 9,000 ppm F (9000F), and 12,300 ppm F (Acid gel). The gels were applied in a thin layer for one minute. Half of the blocks were subjected to pH cycling for six days, whereas the remaining specimens were used for loosely- (calcium fluoride; CaF2) and firmly-bound (fluorapatite; FA) fluoride analysis. The percentage of surface hardness recovery (%SHR), area of subsurface lesion (ΔKHN), CaF2, FA, calcium (Ca), and phosphorus (P) on/in enamel were determined. Data (log10-transformed) were subjected to ANOVA and the Student-Newman-Keuls’ test (p<0.05). Results: We observed a dose-response relation between F concentrations in the gels without TMP for %SHR and ΔKHN. The 2.5% Nano and 5% Micro reached similar %SHR when compared with 9000F and Acid gels. For ΔKHN, Placebo and 5% Nano gels had the highest values, and 5% Micro, 2.5% Nano, 9000F, and Acid gels, the lowest. All groups had similar retained CaF2 values, except for Placebo and Acid gel. We verified observed an increase in Ca concentrations in nano-sized TMP groups. Regarding P, TMP groups showed similar formation and retention to 9000F and Acid. Conclusion: Adding 2.5% nano-sized or 5% micrometric TMP to low-fluoride gels lead to enhanced in vitro remineralization of artificial caries lesions.
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spelling Effect of fluoride gels with nano-sized sodium trimetaphosphate on the in vitro remineralization of caries lesionsFluoridesPhosphatesDental EnamelNanoparticlesNanotechnologyObjective: To evaluate the effects of fluoride (F) gels supplemented with micrometric or nano-sized sodium trimetaphosphate (TMPmicro and TMPnano, respectively) on the in vitro remineralization of caries-like lesions. Methodology: Bovine enamel subsurface lesions (n=168) were selected according to their surface hardness (SH) and randomly divided into seven groups (n=24/group): Placebo (without F/TMP), 4,500 ppm F (4500F), 4500F + 2.5% TMPnano (2.5% Nano), 4500F + 5% TMPnano (5% Nano), 4500F + 5% TMPmicro (5% Micro), 9,000 ppm F (9000F), and 12,300 ppm F (Acid gel). The gels were applied in a thin layer for one minute. Half of the blocks were subjected to pH cycling for six days, whereas the remaining specimens were used for loosely- (calcium fluoride; CaF2) and firmly-bound (fluorapatite; FA) fluoride analysis. The percentage of surface hardness recovery (%SHR), area of subsurface lesion (ΔKHN), CaF2, FA, calcium (Ca), and phosphorus (P) on/in enamel were determined. Data (log10-transformed) were subjected to ANOVA and the Student-Newman-Keuls’ test (p<0.05). Results: We observed a dose-response relation between F concentrations in the gels without TMP for %SHR and ΔKHN. The 2.5% Nano and 5% Micro reached similar %SHR when compared with 9000F and Acid gels. For ΔKHN, Placebo and 5% Nano gels had the highest values, and 5% Micro, 2.5% Nano, 9000F, and Acid gels, the lowest. All groups had similar retained CaF2 values, except for Placebo and Acid gel. We verified observed an increase in Ca concentrations in nano-sized TMP groups. Regarding P, TMP groups showed similar formation and retention to 9000F and Acid. Conclusion: Adding 2.5% nano-sized or 5% micrometric TMP to low-fluoride gels lead to enhanced in vitro remineralization of artificial caries lesions.Universidade de São Paulo. Faculdade de Odontologia de Bauru2023-06-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/21381110.1590/1678-7757-2023-0115Journal of Applied Oral Science; Vol. 31 (2023); e20230115Journal of Applied Oral Science; Vol. 31 (2023); e20230115Journal of Applied Oral Science; v. 31 (2023); e202301151678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/213811/195980Copyright (c) 2023 Journal of Applied Oral Sciencehttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessNagata, Mariana EmiDelbem, Alberto Carlos BotazzoBáez-Quintero, Liliana CarolinaDanelon, MarcelleSampaio, CaioMonteiro, Douglas RobertoWiegand, AnnettePessan, Juliano Pelim2023-06-28T18:30:12Zoai:revistas.usp.br:article/213811Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2023-06-28T18:30:12Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Effect of fluoride gels with nano-sized sodium trimetaphosphate on the in vitro remineralization of caries lesions
title Effect of fluoride gels with nano-sized sodium trimetaphosphate on the in vitro remineralization of caries lesions
spellingShingle Effect of fluoride gels with nano-sized sodium trimetaphosphate on the in vitro remineralization of caries lesions
Nagata, Mariana Emi
Fluorides
Phosphates
Dental Enamel
Nanoparticles
Nanotechnology
title_short Effect of fluoride gels with nano-sized sodium trimetaphosphate on the in vitro remineralization of caries lesions
title_full Effect of fluoride gels with nano-sized sodium trimetaphosphate on the in vitro remineralization of caries lesions
title_fullStr Effect of fluoride gels with nano-sized sodium trimetaphosphate on the in vitro remineralization of caries lesions
title_full_unstemmed Effect of fluoride gels with nano-sized sodium trimetaphosphate on the in vitro remineralization of caries lesions
title_sort Effect of fluoride gels with nano-sized sodium trimetaphosphate on the in vitro remineralization of caries lesions
author Nagata, Mariana Emi
author_facet Nagata, Mariana Emi
Delbem, Alberto Carlos Botazzo
Báez-Quintero, Liliana Carolina
Danelon, Marcelle
Sampaio, Caio
Monteiro, Douglas Roberto
Wiegand, Annette
Pessan, Juliano Pelim
author_role author
author2 Delbem, Alberto Carlos Botazzo
Báez-Quintero, Liliana Carolina
Danelon, Marcelle
Sampaio, Caio
Monteiro, Douglas Roberto
Wiegand, Annette
Pessan, Juliano Pelim
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Nagata, Mariana Emi
Delbem, Alberto Carlos Botazzo
Báez-Quintero, Liliana Carolina
Danelon, Marcelle
Sampaio, Caio
Monteiro, Douglas Roberto
Wiegand, Annette
Pessan, Juliano Pelim
dc.subject.por.fl_str_mv Fluorides
Phosphates
Dental Enamel
Nanoparticles
Nanotechnology
topic Fluorides
Phosphates
Dental Enamel
Nanoparticles
Nanotechnology
description Objective: To evaluate the effects of fluoride (F) gels supplemented with micrometric or nano-sized sodium trimetaphosphate (TMPmicro and TMPnano, respectively) on the in vitro remineralization of caries-like lesions. Methodology: Bovine enamel subsurface lesions (n=168) were selected according to their surface hardness (SH) and randomly divided into seven groups (n=24/group): Placebo (without F/TMP), 4,500 ppm F (4500F), 4500F + 2.5% TMPnano (2.5% Nano), 4500F + 5% TMPnano (5% Nano), 4500F + 5% TMPmicro (5% Micro), 9,000 ppm F (9000F), and 12,300 ppm F (Acid gel). The gels were applied in a thin layer for one minute. Half of the blocks were subjected to pH cycling for six days, whereas the remaining specimens were used for loosely- (calcium fluoride; CaF2) and firmly-bound (fluorapatite; FA) fluoride analysis. The percentage of surface hardness recovery (%SHR), area of subsurface lesion (ΔKHN), CaF2, FA, calcium (Ca), and phosphorus (P) on/in enamel were determined. Data (log10-transformed) were subjected to ANOVA and the Student-Newman-Keuls’ test (p<0.05). Results: We observed a dose-response relation between F concentrations in the gels without TMP for %SHR and ΔKHN. The 2.5% Nano and 5% Micro reached similar %SHR when compared with 9000F and Acid gels. For ΔKHN, Placebo and 5% Nano gels had the highest values, and 5% Micro, 2.5% Nano, 9000F, and Acid gels, the lowest. All groups had similar retained CaF2 values, except for Placebo and Acid gel. We verified observed an increase in Ca concentrations in nano-sized TMP groups. Regarding P, TMP groups showed similar formation and retention to 9000F and Acid. Conclusion: Adding 2.5% nano-sized or 5% micrometric TMP to low-fluoride gels lead to enhanced in vitro remineralization of artificial caries lesions.
publishDate 2023
dc.date.none.fl_str_mv 2023-06-28
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/jaos/article/view/213811
10.1590/1678-7757-2023-0115
url https://www.revistas.usp.br/jaos/article/view/213811
identifier_str_mv 10.1590/1678-7757-2023-0115
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/jaos/article/view/213811/195980
dc.rights.driver.fl_str_mv Copyright (c) 2023 Journal of Applied Oral Science
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2023 Journal of Applied Oral Science
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
dc.source.none.fl_str_mv Journal of Applied Oral Science; Vol. 31 (2023); e20230115
Journal of Applied Oral Science; Vol. 31 (2023); e20230115
Journal of Applied Oral Science; v. 31 (2023); e20230115
1678-7765
1678-7757
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
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