Effect of fluoride gels with nano-sized sodium trimetaphosphate on the in vitro remineralization of caries lesions
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/213811 |
Resumo: | Objective: To evaluate the effects of fluoride (F) gels supplemented with micrometric or nano-sized sodium trimetaphosphate (TMPmicro and TMPnano, respectively) on the in vitro remineralization of caries-like lesions. Methodology: Bovine enamel subsurface lesions (n=168) were selected according to their surface hardness (SH) and randomly divided into seven groups (n=24/group): Placebo (without F/TMP), 4,500 ppm F (4500F), 4500F + 2.5% TMPnano (2.5% Nano), 4500F + 5% TMPnano (5% Nano), 4500F + 5% TMPmicro (5% Micro), 9,000 ppm F (9000F), and 12,300 ppm F (Acid gel). The gels were applied in a thin layer for one minute. Half of the blocks were subjected to pH cycling for six days, whereas the remaining specimens were used for loosely- (calcium fluoride; CaF2) and firmly-bound (fluorapatite; FA) fluoride analysis. The percentage of surface hardness recovery (%SHR), area of subsurface lesion (ΔKHN), CaF2, FA, calcium (Ca), and phosphorus (P) on/in enamel were determined. Data (log10-transformed) were subjected to ANOVA and the Student-Newman-Keuls’ test (p<0.05). Results: We observed a dose-response relation between F concentrations in the gels without TMP for %SHR and ΔKHN. The 2.5% Nano and 5% Micro reached similar %SHR when compared with 9000F and Acid gels. For ΔKHN, Placebo and 5% Nano gels had the highest values, and 5% Micro, 2.5% Nano, 9000F, and Acid gels, the lowest. All groups had similar retained CaF2 values, except for Placebo and Acid gel. We verified observed an increase in Ca concentrations in nano-sized TMP groups. Regarding P, TMP groups showed similar formation and retention to 9000F and Acid. Conclusion: Adding 2.5% nano-sized or 5% micrometric TMP to low-fluoride gels lead to enhanced in vitro remineralization of artificial caries lesions. |
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Journal of applied oral science (Online) |
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Effect of fluoride gels with nano-sized sodium trimetaphosphate on the in vitro remineralization of caries lesionsFluoridesPhosphatesDental EnamelNanoparticlesNanotechnologyObjective: To evaluate the effects of fluoride (F) gels supplemented with micrometric or nano-sized sodium trimetaphosphate (TMPmicro and TMPnano, respectively) on the in vitro remineralization of caries-like lesions. Methodology: Bovine enamel subsurface lesions (n=168) were selected according to their surface hardness (SH) and randomly divided into seven groups (n=24/group): Placebo (without F/TMP), 4,500 ppm F (4500F), 4500F + 2.5% TMPnano (2.5% Nano), 4500F + 5% TMPnano (5% Nano), 4500F + 5% TMPmicro (5% Micro), 9,000 ppm F (9000F), and 12,300 ppm F (Acid gel). The gels were applied in a thin layer for one minute. Half of the blocks were subjected to pH cycling for six days, whereas the remaining specimens were used for loosely- (calcium fluoride; CaF2) and firmly-bound (fluorapatite; FA) fluoride analysis. The percentage of surface hardness recovery (%SHR), area of subsurface lesion (ΔKHN), CaF2, FA, calcium (Ca), and phosphorus (P) on/in enamel were determined. Data (log10-transformed) were subjected to ANOVA and the Student-Newman-Keuls’ test (p<0.05). Results: We observed a dose-response relation between F concentrations in the gels without TMP for %SHR and ΔKHN. The 2.5% Nano and 5% Micro reached similar %SHR when compared with 9000F and Acid gels. For ΔKHN, Placebo and 5% Nano gels had the highest values, and 5% Micro, 2.5% Nano, 9000F, and Acid gels, the lowest. All groups had similar retained CaF2 values, except for Placebo and Acid gel. We verified observed an increase in Ca concentrations in nano-sized TMP groups. Regarding P, TMP groups showed similar formation and retention to 9000F and Acid. Conclusion: Adding 2.5% nano-sized or 5% micrometric TMP to low-fluoride gels lead to enhanced in vitro remineralization of artificial caries lesions.Universidade de São Paulo. Faculdade de Odontologia de Bauru2023-06-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/21381110.1590/1678-7757-2023-0115Journal of Applied Oral Science; Vol. 31 (2023); e20230115Journal of Applied Oral Science; Vol. 31 (2023); e20230115Journal of Applied Oral Science; v. 31 (2023); e202301151678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/213811/195980Copyright (c) 2023 Journal of Applied Oral Sciencehttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessNagata, Mariana EmiDelbem, Alberto Carlos BotazzoBáez-Quintero, Liliana CarolinaDanelon, MarcelleSampaio, CaioMonteiro, Douglas RobertoWiegand, AnnettePessan, Juliano Pelim2023-06-28T18:30:12Zoai:revistas.usp.br:article/213811Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2023-06-28T18:30:12Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Effect of fluoride gels with nano-sized sodium trimetaphosphate on the in vitro remineralization of caries lesions |
title |
Effect of fluoride gels with nano-sized sodium trimetaphosphate on the in vitro remineralization of caries lesions |
spellingShingle |
Effect of fluoride gels with nano-sized sodium trimetaphosphate on the in vitro remineralization of caries lesions Nagata, Mariana Emi Fluorides Phosphates Dental Enamel Nanoparticles Nanotechnology |
title_short |
Effect of fluoride gels with nano-sized sodium trimetaphosphate on the in vitro remineralization of caries lesions |
title_full |
Effect of fluoride gels with nano-sized sodium trimetaphosphate on the in vitro remineralization of caries lesions |
title_fullStr |
Effect of fluoride gels with nano-sized sodium trimetaphosphate on the in vitro remineralization of caries lesions |
title_full_unstemmed |
Effect of fluoride gels with nano-sized sodium trimetaphosphate on the in vitro remineralization of caries lesions |
title_sort |
Effect of fluoride gels with nano-sized sodium trimetaphosphate on the in vitro remineralization of caries lesions |
author |
Nagata, Mariana Emi |
author_facet |
Nagata, Mariana Emi Delbem, Alberto Carlos Botazzo Báez-Quintero, Liliana Carolina Danelon, Marcelle Sampaio, Caio Monteiro, Douglas Roberto Wiegand, Annette Pessan, Juliano Pelim |
author_role |
author |
author2 |
Delbem, Alberto Carlos Botazzo Báez-Quintero, Liliana Carolina Danelon, Marcelle Sampaio, Caio Monteiro, Douglas Roberto Wiegand, Annette Pessan, Juliano Pelim |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Nagata, Mariana Emi Delbem, Alberto Carlos Botazzo Báez-Quintero, Liliana Carolina Danelon, Marcelle Sampaio, Caio Monteiro, Douglas Roberto Wiegand, Annette Pessan, Juliano Pelim |
dc.subject.por.fl_str_mv |
Fluorides Phosphates Dental Enamel Nanoparticles Nanotechnology |
topic |
Fluorides Phosphates Dental Enamel Nanoparticles Nanotechnology |
description |
Objective: To evaluate the effects of fluoride (F) gels supplemented with micrometric or nano-sized sodium trimetaphosphate (TMPmicro and TMPnano, respectively) on the in vitro remineralization of caries-like lesions. Methodology: Bovine enamel subsurface lesions (n=168) were selected according to their surface hardness (SH) and randomly divided into seven groups (n=24/group): Placebo (without F/TMP), 4,500 ppm F (4500F), 4500F + 2.5% TMPnano (2.5% Nano), 4500F + 5% TMPnano (5% Nano), 4500F + 5% TMPmicro (5% Micro), 9,000 ppm F (9000F), and 12,300 ppm F (Acid gel). The gels were applied in a thin layer for one minute. Half of the blocks were subjected to pH cycling for six days, whereas the remaining specimens were used for loosely- (calcium fluoride; CaF2) and firmly-bound (fluorapatite; FA) fluoride analysis. The percentage of surface hardness recovery (%SHR), area of subsurface lesion (ΔKHN), CaF2, FA, calcium (Ca), and phosphorus (P) on/in enamel were determined. Data (log10-transformed) were subjected to ANOVA and the Student-Newman-Keuls’ test (p<0.05). Results: We observed a dose-response relation between F concentrations in the gels without TMP for %SHR and ΔKHN. The 2.5% Nano and 5% Micro reached similar %SHR when compared with 9000F and Acid gels. For ΔKHN, Placebo and 5% Nano gels had the highest values, and 5% Micro, 2.5% Nano, 9000F, and Acid gels, the lowest. All groups had similar retained CaF2 values, except for Placebo and Acid gel. We verified observed an increase in Ca concentrations in nano-sized TMP groups. Regarding P, TMP groups showed similar formation and retention to 9000F and Acid. Conclusion: Adding 2.5% nano-sized or 5% micrometric TMP to low-fluoride gels lead to enhanced in vitro remineralization of artificial caries lesions. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-06-28 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/213811 10.1590/1678-7757-2023-0115 |
url |
https://www.revistas.usp.br/jaos/article/view/213811 |
identifier_str_mv |
10.1590/1678-7757-2023-0115 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/213811/195980 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2023 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2023 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 31 (2023); e20230115 Journal of Applied Oral Science; Vol. 31 (2023); e20230115 Journal of Applied Oral Science; v. 31 (2023); e20230115 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221683578044416 |