Surface morphology and in vitro leachability of soft liners modified by the incorporation of antifungals for denture stomatitis treatment
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/190845 |
Resumo: | Objective: To evaluate the surface morphology and in vitro leachability of temporary soft linings modified by the incorporation of antifungals in minimum inhibitory concentrations (MIC) for Candida albicans biofilm. Methodology: Specimens of soft lining materials Softone and Trusoft were made without (control) or with the addition of nystatin (Ny), miconazole (Mc), ketoconazole (Ke), chlorhexidine diacetate (Chx), or itraconazole (It) at their MIC for C. albicans biofilm. The surface analyses were performed using Confocal laser scanning microscopy after 24 h, 7 days, or 14 days of immersion in distilled water at 37ºC. In vitro leachability of Chx or Ny from the modified materials was also measured using Ultraviolet visible spectroscopy for up to 14 days of immersion in distilled water at 37ºC. Data (μg/mL) were submitted to ANOVA 1-factor/Bonferroni (α=0.05). Results: Softone had a more irregular surface than Trusoft. Morphological changes were noted in both materials with increasing immersion time, particularly, in those containing drugs. Groups containing Chx and It presented extremely porous and irregular surfaces. Both materials had biexponential release kinetics. Softone leached a higher concentration of the antifungals than Trusoft (p=0.004), and chlorhexidine was released at a higher concentration than nystatin (p<0.001). Conclusions: The surface of the soft lining materials changed more significantly with the addition of Chx or It. Softone released a higher concentration of drugs than Trusoft did, guiding the future treatment of denture stomatitis. |
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Journal of applied oral science (Online) |
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Surface morphology and in vitro leachability of soft liners modified by the incorporation of antifungals for denture stomatitis treatmentAnti-infective agentsDrug releaseDenture linersStomatitis, dentureSurface propertiesObjective: To evaluate the surface morphology and in vitro leachability of temporary soft linings modified by the incorporation of antifungals in minimum inhibitory concentrations (MIC) for Candida albicans biofilm. Methodology: Specimens of soft lining materials Softone and Trusoft were made without (control) or with the addition of nystatin (Ny), miconazole (Mc), ketoconazole (Ke), chlorhexidine diacetate (Chx), or itraconazole (It) at their MIC for C. albicans biofilm. The surface analyses were performed using Confocal laser scanning microscopy after 24 h, 7 days, or 14 days of immersion in distilled water at 37ºC. In vitro leachability of Chx or Ny from the modified materials was also measured using Ultraviolet visible spectroscopy for up to 14 days of immersion in distilled water at 37ºC. Data (μg/mL) were submitted to ANOVA 1-factor/Bonferroni (α=0.05). Results: Softone had a more irregular surface than Trusoft. Morphological changes were noted in both materials with increasing immersion time, particularly, in those containing drugs. Groups containing Chx and It presented extremely porous and irregular surfaces. Both materials had biexponential release kinetics. Softone leached a higher concentration of the antifungals than Trusoft (p=0.004), and chlorhexidine was released at a higher concentration than nystatin (p<0.001). Conclusions: The surface of the soft lining materials changed more significantly with the addition of Chx or It. Softone released a higher concentration of drugs than Trusoft did, guiding the future treatment of denture stomatitis.Universidade de São Paulo. Faculdade de Odontologia de Bauru2021-09-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/19084510.1590/1678-7757-2020-0639Journal of Applied Oral Science; Vol. 28 (2020); e20200639Journal of Applied Oral Science; Vol. 28 (2020); e20200639Journal of Applied Oral Science; v. 28 (2020); e202006391678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/190845/175990Copyright (c) 2021 Journal of Applied Oral Sciencehttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSánchez-Aliaga, AdelaidaFarago, Paulo VitorMichél, Milton Domingos Sugio, Carolina Yoshi Campos Neppelenbroek, Karin Hermana Urban, Vanessa Migliorini 2021-09-22T14:31:06Zoai:revistas.usp.br:article/190845Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2021-09-22T14:31:06Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Surface morphology and in vitro leachability of soft liners modified by the incorporation of antifungals for denture stomatitis treatment |
title |
Surface morphology and in vitro leachability of soft liners modified by the incorporation of antifungals for denture stomatitis treatment |
spellingShingle |
Surface morphology and in vitro leachability of soft liners modified by the incorporation of antifungals for denture stomatitis treatment Sánchez-Aliaga, Adelaida Anti-infective agents Drug release Denture liners Stomatitis, denture Surface properties |
title_short |
Surface morphology and in vitro leachability of soft liners modified by the incorporation of antifungals for denture stomatitis treatment |
title_full |
Surface morphology and in vitro leachability of soft liners modified by the incorporation of antifungals for denture stomatitis treatment |
title_fullStr |
Surface morphology and in vitro leachability of soft liners modified by the incorporation of antifungals for denture stomatitis treatment |
title_full_unstemmed |
Surface morphology and in vitro leachability of soft liners modified by the incorporation of antifungals for denture stomatitis treatment |
title_sort |
Surface morphology and in vitro leachability of soft liners modified by the incorporation of antifungals for denture stomatitis treatment |
author |
Sánchez-Aliaga, Adelaida |
author_facet |
Sánchez-Aliaga, Adelaida Farago, Paulo Vitor Michél, Milton Domingos Sugio, Carolina Yoshi Campos Neppelenbroek, Karin Hermana Urban, Vanessa Migliorini |
author_role |
author |
author2 |
Farago, Paulo Vitor Michél, Milton Domingos Sugio, Carolina Yoshi Campos Neppelenbroek, Karin Hermana Urban, Vanessa Migliorini |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Sánchez-Aliaga, Adelaida Farago, Paulo Vitor Michél, Milton Domingos Sugio, Carolina Yoshi Campos Neppelenbroek, Karin Hermana Urban, Vanessa Migliorini |
dc.subject.por.fl_str_mv |
Anti-infective agents Drug release Denture liners Stomatitis, denture Surface properties |
topic |
Anti-infective agents Drug release Denture liners Stomatitis, denture Surface properties |
description |
Objective: To evaluate the surface morphology and in vitro leachability of temporary soft linings modified by the incorporation of antifungals in minimum inhibitory concentrations (MIC) for Candida albicans biofilm. Methodology: Specimens of soft lining materials Softone and Trusoft were made without (control) or with the addition of nystatin (Ny), miconazole (Mc), ketoconazole (Ke), chlorhexidine diacetate (Chx), or itraconazole (It) at their MIC for C. albicans biofilm. The surface analyses were performed using Confocal laser scanning microscopy after 24 h, 7 days, or 14 days of immersion in distilled water at 37ºC. In vitro leachability of Chx or Ny from the modified materials was also measured using Ultraviolet visible spectroscopy for up to 14 days of immersion in distilled water at 37ºC. Data (μg/mL) were submitted to ANOVA 1-factor/Bonferroni (α=0.05). Results: Softone had a more irregular surface than Trusoft. Morphological changes were noted in both materials with increasing immersion time, particularly, in those containing drugs. Groups containing Chx and It presented extremely porous and irregular surfaces. Both materials had biexponential release kinetics. Softone leached a higher concentration of the antifungals than Trusoft (p=0.004), and chlorhexidine was released at a higher concentration than nystatin (p<0.001). Conclusions: The surface of the soft lining materials changed more significantly with the addition of Chx or It. Softone released a higher concentration of drugs than Trusoft did, guiding the future treatment of denture stomatitis. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-09-22 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/190845 10.1590/1678-7757-2020-0639 |
url |
https://www.revistas.usp.br/jaos/article/view/190845 |
identifier_str_mv |
10.1590/1678-7757-2020-0639 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/190845/175990 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 28 (2020); e20200639 Journal of Applied Oral Science; Vol. 28 (2020); e20200639 Journal of Applied Oral Science; v. 28 (2020); e20200639 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221682485428224 |