Effects of CEACAM1 in oral keratinocytes on HO-1 expression induced by Candida β-glucan particles

Detalhes bibliográficos
Autor(a) principal: SAKUMA,Miyuki
Data de Publicação: 2022
Outros Autores: OHTA,Kouji, FUKADA,Shohei, AKAGI,Misaki, KATO,Hiroki, ISHIDA,Yoko, NARUSE,Takako, TAKECHI,Masaaki, SHIGEISHI,Hideo, NISHI,Hiromi, AIKAWA,Tomonao
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572022000100442
Resumo: Abstract Objective Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is a member of the carcinoembryonic antigen family. Although its expression has been found in chronic oral inflammatory epithelium, this study aimed to know whether CEACAM1 in oral keratinocytes participates in host immune response against Candida albicans . Methodology We investigated CEACAM1 expression in oral keratinocytes induced by C. albicans as well as by Candida cell wall component β-glucan particles (β-GPs). Furthermore, the effects of CEACAM1 on β-GPs-induced heme oxygenase-1 (HO-1) expression and its related signals were examined. Results Fluorescence staining showed CEACAM1 expression in oral keratinocytes (RT7) cells, whereas quantitative reverse transcription (RT)-PCR indicated that both live and heat-killed C. albicans increased CEACAM1 mRNA expression in RT7 cells. Examinations using quantitative RT-PCR and western blotting indicated that CEACAM1 expression was also increased by β-GPs derived from C. albicans . Specific siRNA for CEACAM1 decreased HO-1 expression induced by β-GPs from C. albicans as well as the budding yeast microorganism Saccharomyces cerevisiae . Moreover, knockdown of CEACAM1 decreased β-GPs-induced ROS activity in the early phase and translocation of Nrf2 into the nucleus. Conclusion CEACAM1 in oral keratinocytes may have a critical role in regulation of HO-1 for host immune defense during Candida infection.
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network_name_str Journal of applied oral science (Online)
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spelling Effects of CEACAM1 in oral keratinocytes on HO-1 expression induced by Candida β-glucan particlesCandida albicansCEACAM1β-glucan-containing particlesHO-1Oral keratinocytesAbstract Objective Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is a member of the carcinoembryonic antigen family. Although its expression has been found in chronic oral inflammatory epithelium, this study aimed to know whether CEACAM1 in oral keratinocytes participates in host immune response against Candida albicans . Methodology We investigated CEACAM1 expression in oral keratinocytes induced by C. albicans as well as by Candida cell wall component β-glucan particles (β-GPs). Furthermore, the effects of CEACAM1 on β-GPs-induced heme oxygenase-1 (HO-1) expression and its related signals were examined. Results Fluorescence staining showed CEACAM1 expression in oral keratinocytes (RT7) cells, whereas quantitative reverse transcription (RT)-PCR indicated that both live and heat-killed C. albicans increased CEACAM1 mRNA expression in RT7 cells. Examinations using quantitative RT-PCR and western blotting indicated that CEACAM1 expression was also increased by β-GPs derived from C. albicans . Specific siRNA for CEACAM1 decreased HO-1 expression induced by β-GPs from C. albicans as well as the budding yeast microorganism Saccharomyces cerevisiae . Moreover, knockdown of CEACAM1 decreased β-GPs-induced ROS activity in the early phase and translocation of Nrf2 into the nucleus. Conclusion CEACAM1 in oral keratinocytes may have a critical role in regulation of HO-1 for host immune defense during Candida infection.Faculdade De Odontologia De Bauru - USP2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572022000100442Journal of Applied Oral Science v.30 2022reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/1678-7757-2022-0158info:eu-repo/semantics/openAccessSAKUMA,MiyukiOHTA,KoujiFUKADA,ShoheiAKAGI,MisakiKATO,HirokiISHIDA,YokoNARUSE,TakakoTAKECHI,MasaakiSHIGEISHI,HideoNISHI,HiromiAIKAWA,Tomonaoeng2022-11-04T00:00:00Zoai:scielo:S1678-77572022000100442Revistahttp://www.scielo.br/jaosPUBhttps://old.scielo.br/oai/scielo-oai.php||jaos@usp.br1678-77651678-7757opendoar:2022-11-04T00:00Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Effects of CEACAM1 in oral keratinocytes on HO-1 expression induced by Candida β-glucan particles
title Effects of CEACAM1 in oral keratinocytes on HO-1 expression induced by Candida β-glucan particles
spellingShingle Effects of CEACAM1 in oral keratinocytes on HO-1 expression induced by Candida β-glucan particles
SAKUMA,Miyuki
Candida albicans
CEACAM1
β-glucan-containing particles
HO-1
Oral keratinocytes
title_short Effects of CEACAM1 in oral keratinocytes on HO-1 expression induced by Candida β-glucan particles
title_full Effects of CEACAM1 in oral keratinocytes on HO-1 expression induced by Candida β-glucan particles
title_fullStr Effects of CEACAM1 in oral keratinocytes on HO-1 expression induced by Candida β-glucan particles
title_full_unstemmed Effects of CEACAM1 in oral keratinocytes on HO-1 expression induced by Candida β-glucan particles
title_sort Effects of CEACAM1 in oral keratinocytes on HO-1 expression induced by Candida β-glucan particles
author SAKUMA,Miyuki
author_facet SAKUMA,Miyuki
OHTA,Kouji
FUKADA,Shohei
AKAGI,Misaki
KATO,Hiroki
ISHIDA,Yoko
NARUSE,Takako
TAKECHI,Masaaki
SHIGEISHI,Hideo
NISHI,Hiromi
AIKAWA,Tomonao
author_role author
author2 OHTA,Kouji
FUKADA,Shohei
AKAGI,Misaki
KATO,Hiroki
ISHIDA,Yoko
NARUSE,Takako
TAKECHI,Masaaki
SHIGEISHI,Hideo
NISHI,Hiromi
AIKAWA,Tomonao
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv SAKUMA,Miyuki
OHTA,Kouji
FUKADA,Shohei
AKAGI,Misaki
KATO,Hiroki
ISHIDA,Yoko
NARUSE,Takako
TAKECHI,Masaaki
SHIGEISHI,Hideo
NISHI,Hiromi
AIKAWA,Tomonao
dc.subject.por.fl_str_mv Candida albicans
CEACAM1
β-glucan-containing particles
HO-1
Oral keratinocytes
topic Candida albicans
CEACAM1
β-glucan-containing particles
HO-1
Oral keratinocytes
description Abstract Objective Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is a member of the carcinoembryonic antigen family. Although its expression has been found in chronic oral inflammatory epithelium, this study aimed to know whether CEACAM1 in oral keratinocytes participates in host immune response against Candida albicans . Methodology We investigated CEACAM1 expression in oral keratinocytes induced by C. albicans as well as by Candida cell wall component β-glucan particles (β-GPs). Furthermore, the effects of CEACAM1 on β-GPs-induced heme oxygenase-1 (HO-1) expression and its related signals were examined. Results Fluorescence staining showed CEACAM1 expression in oral keratinocytes (RT7) cells, whereas quantitative reverse transcription (RT)-PCR indicated that both live and heat-killed C. albicans increased CEACAM1 mRNA expression in RT7 cells. Examinations using quantitative RT-PCR and western blotting indicated that CEACAM1 expression was also increased by β-GPs derived from C. albicans . Specific siRNA for CEACAM1 decreased HO-1 expression induced by β-GPs from C. albicans as well as the budding yeast microorganism Saccharomyces cerevisiae . Moreover, knockdown of CEACAM1 decreased β-GPs-induced ROS activity in the early phase and translocation of Nrf2 into the nucleus. Conclusion CEACAM1 in oral keratinocytes may have a critical role in regulation of HO-1 for host immune defense during Candida infection.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572022000100442
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572022000100442
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-7757-2022-0158
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Faculdade De Odontologia De Bauru - USP
publisher.none.fl_str_mv Faculdade De Odontologia De Bauru - USP
dc.source.none.fl_str_mv Journal of Applied Oral Science v.30 2022
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
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