Dynamics and metabolic profile of oral keratinocytes (NOK-si) and Candida albicans after interaction in co-culture

Detalhes bibliográficos
Autor(a) principal: Masetti, Paula [UNESP]
Data de Publicação: 2021
Outros Autores: Sanitá, Paula Volpato [UNESP], Jorge, Janaina Habib [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1080/08927014.2021.1941908
http://hdl.handle.net/11449/221915
Resumo: Understanding the interaction between oral keratinocytes (NOK-si) and Candida albicans is fundamental for the development of prevention strategies and new therapies for oral candidiasis. This study evaluated the dynamics and metabolic profile of these cells growing in co-culture by means of cell metabolism, number of CFU ml−1, and production of enzymes, cytokines, and metabolites. The data were analyzed by ANOVAs and post hoc tests (α = 0.05). In co-cultures, there were significant decreases in the cell metabolism of NOK-si and C. albicans and increases in the CFU ml−1 values of C. albicans biofilm. There were also significant increases in the production of cytokines by NOK-si and proteinase by C. albicans biofilm after their interaction. The metabolic balance of the main metabolites, amino acids, and extracellular and intracellular metabolites was shifted in favor of the co-cultures, while aromatic alcohols were secreted in higher amounts by the biofilm of C. albicans. It was concluded that the interaction of cells in co-culture influenced their dynamics over time.
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spelling Dynamics and metabolic profile of oral keratinocytes (NOK-si) and Candida albicans after interaction in co-cultureCandida albicansco-cultureMetabolic profileoral keratinocytesUnderstanding the interaction between oral keratinocytes (NOK-si) and Candida albicans is fundamental for the development of prevention strategies and new therapies for oral candidiasis. This study evaluated the dynamics and metabolic profile of these cells growing in co-culture by means of cell metabolism, number of CFU ml−1, and production of enzymes, cytokines, and metabolites. The data were analyzed by ANOVAs and post hoc tests (α = 0.05). In co-cultures, there were significant decreases in the cell metabolism of NOK-si and C. albicans and increases in the CFU ml−1 values of C. albicans biofilm. There were also significant increases in the production of cytokines by NOK-si and proteinase by C. albicans biofilm after their interaction. The metabolic balance of the main metabolites, amino acids, and extracellular and intracellular metabolites was shifted in favor of the co-cultures, while aromatic alcohols were secreted in higher amounts by the biofilm of C. albicans. It was concluded that the interaction of cells in co-culture influenced their dynamics over time.Department of Dental Materials and Prosthodontics São Paulo State University (Unesp) School of DentistryDepartment of Dental Materials and Prosthodontics São Paulo State University (Unesp) School of DentistryUniversidade Estadual Paulista (UNESP)Masetti, Paula [UNESP]Sanitá, Paula Volpato [UNESP]Jorge, Janaina Habib [UNESP]2022-04-28T19:41:23Z2022-04-28T19:41:23Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article572-589http://dx.doi.org/10.1080/08927014.2021.1941908Biofouling, v. 37, n. 5, p. 572-589, 2021.1029-24540892-7014http://hdl.handle.net/11449/22191510.1080/08927014.2021.19419082-s2.0-85109389091Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiofoulinginfo:eu-repo/semantics/openAccess2022-04-28T19:41:23Zoai:repositorio.unesp.br:11449/221915Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:05:10.257739Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Dynamics and metabolic profile of oral keratinocytes (NOK-si) and Candida albicans after interaction in co-culture
title Dynamics and metabolic profile of oral keratinocytes (NOK-si) and Candida albicans after interaction in co-culture
spellingShingle Dynamics and metabolic profile of oral keratinocytes (NOK-si) and Candida albicans after interaction in co-culture
Masetti, Paula [UNESP]
Candida albicans
co-culture
Metabolic profile
oral keratinocytes
title_short Dynamics and metabolic profile of oral keratinocytes (NOK-si) and Candida albicans after interaction in co-culture
title_full Dynamics and metabolic profile of oral keratinocytes (NOK-si) and Candida albicans after interaction in co-culture
title_fullStr Dynamics and metabolic profile of oral keratinocytes (NOK-si) and Candida albicans after interaction in co-culture
title_full_unstemmed Dynamics and metabolic profile of oral keratinocytes (NOK-si) and Candida albicans after interaction in co-culture
title_sort Dynamics and metabolic profile of oral keratinocytes (NOK-si) and Candida albicans after interaction in co-culture
author Masetti, Paula [UNESP]
author_facet Masetti, Paula [UNESP]
Sanitá, Paula Volpato [UNESP]
Jorge, Janaina Habib [UNESP]
author_role author
author2 Sanitá, Paula Volpato [UNESP]
Jorge, Janaina Habib [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Masetti, Paula [UNESP]
Sanitá, Paula Volpato [UNESP]
Jorge, Janaina Habib [UNESP]
dc.subject.por.fl_str_mv Candida albicans
co-culture
Metabolic profile
oral keratinocytes
topic Candida albicans
co-culture
Metabolic profile
oral keratinocytes
description Understanding the interaction between oral keratinocytes (NOK-si) and Candida albicans is fundamental for the development of prevention strategies and new therapies for oral candidiasis. This study evaluated the dynamics and metabolic profile of these cells growing in co-culture by means of cell metabolism, number of CFU ml−1, and production of enzymes, cytokines, and metabolites. The data were analyzed by ANOVAs and post hoc tests (α = 0.05). In co-cultures, there were significant decreases in the cell metabolism of NOK-si and C. albicans and increases in the CFU ml−1 values of C. albicans biofilm. There were also significant increases in the production of cytokines by NOK-si and proteinase by C. albicans biofilm after their interaction. The metabolic balance of the main metabolites, amino acids, and extracellular and intracellular metabolites was shifted in favor of the co-cultures, while aromatic alcohols were secreted in higher amounts by the biofilm of C. albicans. It was concluded that the interaction of cells in co-culture influenced their dynamics over time.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-01
2022-04-28T19:41:23Z
2022-04-28T19:41:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1080/08927014.2021.1941908
Biofouling, v. 37, n. 5, p. 572-589, 2021.
1029-2454
0892-7014
http://hdl.handle.net/11449/221915
10.1080/08927014.2021.1941908
2-s2.0-85109389091
url http://dx.doi.org/10.1080/08927014.2021.1941908
http://hdl.handle.net/11449/221915
identifier_str_mv Biofouling, v. 37, n. 5, p. 572-589, 2021.
1029-2454
0892-7014
10.1080/08927014.2021.1941908
2-s2.0-85109389091
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biofouling
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 572-589
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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