Dynamics and metabolic profile of oral keratinocytes (NOK-si) and Candida albicans after interaction in co-culture
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/08927014.2021.1941908 http://hdl.handle.net/11449/221915 |
Resumo: | Understanding the interaction between oral keratinocytes (NOK-si) and Candida albicans is fundamental for the development of prevention strategies and new therapies for oral candidiasis. This study evaluated the dynamics and metabolic profile of these cells growing in co-culture by means of cell metabolism, number of CFU ml−1, and production of enzymes, cytokines, and metabolites. The data were analyzed by ANOVAs and post hoc tests (α = 0.05). In co-cultures, there were significant decreases in the cell metabolism of NOK-si and C. albicans and increases in the CFU ml−1 values of C. albicans biofilm. There were also significant increases in the production of cytokines by NOK-si and proteinase by C. albicans biofilm after their interaction. The metabolic balance of the main metabolites, amino acids, and extracellular and intracellular metabolites was shifted in favor of the co-cultures, while aromatic alcohols were secreted in higher amounts by the biofilm of C. albicans. It was concluded that the interaction of cells in co-culture influenced their dynamics over time. |
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Repositório Institucional da UNESP |
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Dynamics and metabolic profile of oral keratinocytes (NOK-si) and Candida albicans after interaction in co-cultureCandida albicansco-cultureMetabolic profileoral keratinocytesUnderstanding the interaction between oral keratinocytes (NOK-si) and Candida albicans is fundamental for the development of prevention strategies and new therapies for oral candidiasis. This study evaluated the dynamics and metabolic profile of these cells growing in co-culture by means of cell metabolism, number of CFU ml−1, and production of enzymes, cytokines, and metabolites. The data were analyzed by ANOVAs and post hoc tests (α = 0.05). In co-cultures, there were significant decreases in the cell metabolism of NOK-si and C. albicans and increases in the CFU ml−1 values of C. albicans biofilm. There were also significant increases in the production of cytokines by NOK-si and proteinase by C. albicans biofilm after their interaction. The metabolic balance of the main metabolites, amino acids, and extracellular and intracellular metabolites was shifted in favor of the co-cultures, while aromatic alcohols were secreted in higher amounts by the biofilm of C. albicans. It was concluded that the interaction of cells in co-culture influenced their dynamics over time.Department of Dental Materials and Prosthodontics São Paulo State University (Unesp) School of DentistryDepartment of Dental Materials and Prosthodontics São Paulo State University (Unesp) School of DentistryUniversidade Estadual Paulista (UNESP)Masetti, Paula [UNESP]Sanitá, Paula Volpato [UNESP]Jorge, Janaina Habib [UNESP]2022-04-28T19:41:23Z2022-04-28T19:41:23Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article572-589http://dx.doi.org/10.1080/08927014.2021.1941908Biofouling, v. 37, n. 5, p. 572-589, 2021.1029-24540892-7014http://hdl.handle.net/11449/22191510.1080/08927014.2021.19419082-s2.0-85109389091Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiofoulinginfo:eu-repo/semantics/openAccess2022-04-28T19:41:23Zoai:repositorio.unesp.br:11449/221915Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:05:10.257739Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Dynamics and metabolic profile of oral keratinocytes (NOK-si) and Candida albicans after interaction in co-culture |
title |
Dynamics and metabolic profile of oral keratinocytes (NOK-si) and Candida albicans after interaction in co-culture |
spellingShingle |
Dynamics and metabolic profile of oral keratinocytes (NOK-si) and Candida albicans after interaction in co-culture Masetti, Paula [UNESP] Candida albicans co-culture Metabolic profile oral keratinocytes |
title_short |
Dynamics and metabolic profile of oral keratinocytes (NOK-si) and Candida albicans after interaction in co-culture |
title_full |
Dynamics and metabolic profile of oral keratinocytes (NOK-si) and Candida albicans after interaction in co-culture |
title_fullStr |
Dynamics and metabolic profile of oral keratinocytes (NOK-si) and Candida albicans after interaction in co-culture |
title_full_unstemmed |
Dynamics and metabolic profile of oral keratinocytes (NOK-si) and Candida albicans after interaction in co-culture |
title_sort |
Dynamics and metabolic profile of oral keratinocytes (NOK-si) and Candida albicans after interaction in co-culture |
author |
Masetti, Paula [UNESP] |
author_facet |
Masetti, Paula [UNESP] Sanitá, Paula Volpato [UNESP] Jorge, Janaina Habib [UNESP] |
author_role |
author |
author2 |
Sanitá, Paula Volpato [UNESP] Jorge, Janaina Habib [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Masetti, Paula [UNESP] Sanitá, Paula Volpato [UNESP] Jorge, Janaina Habib [UNESP] |
dc.subject.por.fl_str_mv |
Candida albicans co-culture Metabolic profile oral keratinocytes |
topic |
Candida albicans co-culture Metabolic profile oral keratinocytes |
description |
Understanding the interaction between oral keratinocytes (NOK-si) and Candida albicans is fundamental for the development of prevention strategies and new therapies for oral candidiasis. This study evaluated the dynamics and metabolic profile of these cells growing in co-culture by means of cell metabolism, number of CFU ml−1, and production of enzymes, cytokines, and metabolites. The data were analyzed by ANOVAs and post hoc tests (α = 0.05). In co-cultures, there were significant decreases in the cell metabolism of NOK-si and C. albicans and increases in the CFU ml−1 values of C. albicans biofilm. There were also significant increases in the production of cytokines by NOK-si and proteinase by C. albicans biofilm after their interaction. The metabolic balance of the main metabolites, amino acids, and extracellular and intracellular metabolites was shifted in favor of the co-cultures, while aromatic alcohols were secreted in higher amounts by the biofilm of C. albicans. It was concluded that the interaction of cells in co-culture influenced their dynamics over time. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 2022-04-28T19:41:23Z 2022-04-28T19:41:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/08927014.2021.1941908 Biofouling, v. 37, n. 5, p. 572-589, 2021. 1029-2454 0892-7014 http://hdl.handle.net/11449/221915 10.1080/08927014.2021.1941908 2-s2.0-85109389091 |
url |
http://dx.doi.org/10.1080/08927014.2021.1941908 http://hdl.handle.net/11449/221915 |
identifier_str_mv |
Biofouling, v. 37, n. 5, p. 572-589, 2021. 1029-2454 0892-7014 10.1080/08927014.2021.1941908 2-s2.0-85109389091 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biofouling |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
572-589 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129282346582016 |