Microscale extraction method for HPLC carotenoid analysis in vegetable matrices

Detalhes bibliográficos
Autor(a) principal: Pacheco,Sidney
Data de Publicação: 2014
Outros Autores: Peixoto,Fernanda Marques, Borguini,Renata Galhardo, Nascimento,Luzimar da Silva de Mattos do, Bobeda,Claudio Roberto Ribeiro, Santiago,Manuela Cristina Pessanha de Araújo, Godoy,Ronoel Luiz de Oliveira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Scientia Agrícola (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162014000500011
Resumo: In order to generate simple, efficient analytical methods that are also fast, clean, and economical, and are capable of producing reliable results for a large number of samples, a micro scale extraction method for analysis of carotenoids in vegetable matrices was developed. The efficiency of this adapted method was checked by comparing the results obtained from vegetable matrices, based on extraction equivalence, time required and reagents. Six matrices were used: tomato (Solanum lycopersicum L.), carrot (Daucus carota L.), sweet potato with orange pulp (Ipomoea batatas (L.) Lam.), pumpkin (Cucurbita moschata Duch.), watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai) and sweet potato (Ipomoea batatas (L.) Lam.) flour. Quantification of the total carotenoids was made by spectrophotometry. Quantification and determination of carotenoid profiles were formulated by High Performance Liquid Chromatography with photodiode array detection. Microscale extraction was faster, cheaper and cleaner than the commonly used one, and advantageous for analytical laboratories.
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spelling Microscale extraction method for HPLC carotenoid analysis in vegetable matricesβ-caroteneα-carotenelycopeneIn order to generate simple, efficient analytical methods that are also fast, clean, and economical, and are capable of producing reliable results for a large number of samples, a micro scale extraction method for analysis of carotenoids in vegetable matrices was developed. The efficiency of this adapted method was checked by comparing the results obtained from vegetable matrices, based on extraction equivalence, time required and reagents. Six matrices were used: tomato (Solanum lycopersicum L.), carrot (Daucus carota L.), sweet potato with orange pulp (Ipomoea batatas (L.) Lam.), pumpkin (Cucurbita moschata Duch.), watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai) and sweet potato (Ipomoea batatas (L.) Lam.) flour. Quantification of the total carotenoids was made by spectrophotometry. Quantification and determination of carotenoid profiles were formulated by High Performance Liquid Chromatography with photodiode array detection. Microscale extraction was faster, cheaper and cleaner than the commonly used one, and advantageous for analytical laboratories.Escola Superior de Agricultura "Luiz de Queiroz"2014-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162014000500011Scientia Agricola v.71 n.5 2014reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/0103-9016-2013-0402info:eu-repo/semantics/openAccessPacheco,SidneyPeixoto,Fernanda MarquesBorguini,Renata GalhardoNascimento,Luzimar da Silva de Mattos doBobeda,Claudio Roberto RibeiroSantiago,Manuela Cristina Pessanha de AraújoGodoy,Ronoel Luiz de Oliveiraeng2014-10-01T00:00:00Zoai:scielo:S0103-90162014000500011Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2014-10-01T00:00Scientia Agrícola (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Microscale extraction method for HPLC carotenoid analysis in vegetable matrices
title Microscale extraction method for HPLC carotenoid analysis in vegetable matrices
spellingShingle Microscale extraction method for HPLC carotenoid analysis in vegetable matrices
Pacheco,Sidney
β-carotene
α-carotene
lycopene
title_short Microscale extraction method for HPLC carotenoid analysis in vegetable matrices
title_full Microscale extraction method for HPLC carotenoid analysis in vegetable matrices
title_fullStr Microscale extraction method for HPLC carotenoid analysis in vegetable matrices
title_full_unstemmed Microscale extraction method for HPLC carotenoid analysis in vegetable matrices
title_sort Microscale extraction method for HPLC carotenoid analysis in vegetable matrices
author Pacheco,Sidney
author_facet Pacheco,Sidney
Peixoto,Fernanda Marques
Borguini,Renata Galhardo
Nascimento,Luzimar da Silva de Mattos do
Bobeda,Claudio Roberto Ribeiro
Santiago,Manuela Cristina Pessanha de Araújo
Godoy,Ronoel Luiz de Oliveira
author_role author
author2 Peixoto,Fernanda Marques
Borguini,Renata Galhardo
Nascimento,Luzimar da Silva de Mattos do
Bobeda,Claudio Roberto Ribeiro
Santiago,Manuela Cristina Pessanha de Araújo
Godoy,Ronoel Luiz de Oliveira
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pacheco,Sidney
Peixoto,Fernanda Marques
Borguini,Renata Galhardo
Nascimento,Luzimar da Silva de Mattos do
Bobeda,Claudio Roberto Ribeiro
Santiago,Manuela Cristina Pessanha de Araújo
Godoy,Ronoel Luiz de Oliveira
dc.subject.por.fl_str_mv β-carotene
α-carotene
lycopene
topic β-carotene
α-carotene
lycopene
description In order to generate simple, efficient analytical methods that are also fast, clean, and economical, and are capable of producing reliable results for a large number of samples, a micro scale extraction method for analysis of carotenoids in vegetable matrices was developed. The efficiency of this adapted method was checked by comparing the results obtained from vegetable matrices, based on extraction equivalence, time required and reagents. Six matrices were used: tomato (Solanum lycopersicum L.), carrot (Daucus carota L.), sweet potato with orange pulp (Ipomoea batatas (L.) Lam.), pumpkin (Cucurbita moschata Duch.), watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai) and sweet potato (Ipomoea batatas (L.) Lam.) flour. Quantification of the total carotenoids was made by spectrophotometry. Quantification and determination of carotenoid profiles were formulated by High Performance Liquid Chromatography with photodiode array detection. Microscale extraction was faster, cheaper and cleaner than the commonly used one, and advantageous for analytical laboratories.
publishDate 2014
dc.date.none.fl_str_mv 2014-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162014000500011
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162014000500011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0103-9016-2013-0402
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Escola Superior de Agricultura "Luiz de Queiroz"
publisher.none.fl_str_mv Escola Superior de Agricultura "Luiz de Queiroz"
dc.source.none.fl_str_mv Scientia Agricola v.71 n.5 2014
reponame:Scientia Agrícola (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Scientia Agrícola (Online)
collection Scientia Agrícola (Online)
repository.name.fl_str_mv Scientia Agrícola (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv scientia@usp.br||alleoni@usp.br
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