Microscale extraction method for HPLC carotenoid analysis in vegetable matrices
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Scientia Agrícola (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162014000500011 |
Resumo: | In order to generate simple, efficient analytical methods that are also fast, clean, and economical, and are capable of producing reliable results for a large number of samples, a micro scale extraction method for analysis of carotenoids in vegetable matrices was developed. The efficiency of this adapted method was checked by comparing the results obtained from vegetable matrices, based on extraction equivalence, time required and reagents. Six matrices were used: tomato (Solanum lycopersicum L.), carrot (Daucus carota L.), sweet potato with orange pulp (Ipomoea batatas (L.) Lam.), pumpkin (Cucurbita moschata Duch.), watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai) and sweet potato (Ipomoea batatas (L.) Lam.) flour. Quantification of the total carotenoids was made by spectrophotometry. Quantification and determination of carotenoid profiles were formulated by High Performance Liquid Chromatography with photodiode array detection. Microscale extraction was faster, cheaper and cleaner than the commonly used one, and advantageous for analytical laboratories. |
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Microscale extraction method for HPLC carotenoid analysis in vegetable matricesβ-caroteneα-carotenelycopeneIn order to generate simple, efficient analytical methods that are also fast, clean, and economical, and are capable of producing reliable results for a large number of samples, a micro scale extraction method for analysis of carotenoids in vegetable matrices was developed. The efficiency of this adapted method was checked by comparing the results obtained from vegetable matrices, based on extraction equivalence, time required and reagents. Six matrices were used: tomato (Solanum lycopersicum L.), carrot (Daucus carota L.), sweet potato with orange pulp (Ipomoea batatas (L.) Lam.), pumpkin (Cucurbita moschata Duch.), watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai) and sweet potato (Ipomoea batatas (L.) Lam.) flour. Quantification of the total carotenoids was made by spectrophotometry. Quantification and determination of carotenoid profiles were formulated by High Performance Liquid Chromatography with photodiode array detection. Microscale extraction was faster, cheaper and cleaner than the commonly used one, and advantageous for analytical laboratories.Escola Superior de Agricultura "Luiz de Queiroz"2014-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162014000500011Scientia Agricola v.71 n.5 2014reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/0103-9016-2013-0402info:eu-repo/semantics/openAccessPacheco,SidneyPeixoto,Fernanda MarquesBorguini,Renata GalhardoNascimento,Luzimar da Silva de Mattos doBobeda,Claudio Roberto RibeiroSantiago,Manuela Cristina Pessanha de AraújoGodoy,Ronoel Luiz de Oliveiraeng2014-10-01T00:00:00Zoai:scielo:S0103-90162014000500011Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2014-10-01T00:00Scientia Agrícola (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Microscale extraction method for HPLC carotenoid analysis in vegetable matrices |
title |
Microscale extraction method for HPLC carotenoid analysis in vegetable matrices |
spellingShingle |
Microscale extraction method for HPLC carotenoid analysis in vegetable matrices Pacheco,Sidney β-carotene α-carotene lycopene |
title_short |
Microscale extraction method for HPLC carotenoid analysis in vegetable matrices |
title_full |
Microscale extraction method for HPLC carotenoid analysis in vegetable matrices |
title_fullStr |
Microscale extraction method for HPLC carotenoid analysis in vegetable matrices |
title_full_unstemmed |
Microscale extraction method for HPLC carotenoid analysis in vegetable matrices |
title_sort |
Microscale extraction method for HPLC carotenoid analysis in vegetable matrices |
author |
Pacheco,Sidney |
author_facet |
Pacheco,Sidney Peixoto,Fernanda Marques Borguini,Renata Galhardo Nascimento,Luzimar da Silva de Mattos do Bobeda,Claudio Roberto Ribeiro Santiago,Manuela Cristina Pessanha de Araújo Godoy,Ronoel Luiz de Oliveira |
author_role |
author |
author2 |
Peixoto,Fernanda Marques Borguini,Renata Galhardo Nascimento,Luzimar da Silva de Mattos do Bobeda,Claudio Roberto Ribeiro Santiago,Manuela Cristina Pessanha de Araújo Godoy,Ronoel Luiz de Oliveira |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Pacheco,Sidney Peixoto,Fernanda Marques Borguini,Renata Galhardo Nascimento,Luzimar da Silva de Mattos do Bobeda,Claudio Roberto Ribeiro Santiago,Manuela Cristina Pessanha de Araújo Godoy,Ronoel Luiz de Oliveira |
dc.subject.por.fl_str_mv |
β-carotene α-carotene lycopene |
topic |
β-carotene α-carotene lycopene |
description |
In order to generate simple, efficient analytical methods that are also fast, clean, and economical, and are capable of producing reliable results for a large number of samples, a micro scale extraction method for analysis of carotenoids in vegetable matrices was developed. The efficiency of this adapted method was checked by comparing the results obtained from vegetable matrices, based on extraction equivalence, time required and reagents. Six matrices were used: tomato (Solanum lycopersicum L.), carrot (Daucus carota L.), sweet potato with orange pulp (Ipomoea batatas (L.) Lam.), pumpkin (Cucurbita moschata Duch.), watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai) and sweet potato (Ipomoea batatas (L.) Lam.) flour. Quantification of the total carotenoids was made by spectrophotometry. Quantification and determination of carotenoid profiles were formulated by High Performance Liquid Chromatography with photodiode array detection. Microscale extraction was faster, cheaper and cleaner than the commonly used one, and advantageous for analytical laboratories. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162014000500011 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162014000500011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-9016-2013-0402 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
dc.source.none.fl_str_mv |
Scientia Agricola v.71 n.5 2014 reponame:Scientia Agrícola (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Scientia Agrícola (Online) |
collection |
Scientia Agrícola (Online) |
repository.name.fl_str_mv |
Scientia Agrícola (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
scientia@usp.br||alleoni@usp.br |
_version_ |
1748936463388508160 |