Glutamine treatment attenuates hyperglycemia-induced mitochondrial stress and apoptosis in umbilical vein endothelial cells

Detalhes bibliográficos
Autor(a) principal: Safi, Sher Zaman
Data de Publicação: 2015
Outros Autores: Batumalaie, Kalaivani, Mansor, Marzida, Chinna, Karuthan, Mohan, Syam, Karimian, Hamed, Qvist, Rajes, Ashraf, Muhammad Aqeel, Yan, Garcie Ong Siok
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Clinics
Texto Completo: https://www.revistas.usp.br/clinics/article/view/102016
Resumo: OBJECTIVE: The aim of this study was to determine the in vitro effect of glutamine and insulin on apoptosis, mitochondrial membrane potential, cell permeability, and inflammatory cytokines in hyperglycemic umbilical vein endothelial cells. MATERIALS AND METHODS: Human umbilical vein endothelial cells were grown and subjected to glutamine and insulin to examine the effects of these agents on the hyperglycemic state. Mitochondrial function and the production of inflammatory cytokines were assessed using fluorescence analysis and multiple cytotoxicity assays. Apoptosis was analyzed by the terminal deoxynucleotidyl transferase dUTP nick end-labeling assay. RESULTS: Glutamine maintains the integrity of the mitochondria by reducing the cell permeability and cytochrome c levels and increasing the mitochondrial membrane potential. The cytochrome c level was significantly (p
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spelling Glutamine treatment attenuates hyperglycemia-induced mitochondrial stress and apoptosis in umbilical vein endothelial cells OBJECTIVE: The aim of this study was to determine the in vitro effect of glutamine and insulin on apoptosis, mitochondrial membrane potential, cell permeability, and inflammatory cytokines in hyperglycemic umbilical vein endothelial cells. MATERIALS AND METHODS: Human umbilical vein endothelial cells were grown and subjected to glutamine and insulin to examine the effects of these agents on the hyperglycemic state. Mitochondrial function and the production of inflammatory cytokines were assessed using fluorescence analysis and multiple cytotoxicity assays. Apoptosis was analyzed by the terminal deoxynucleotidyl transferase dUTP nick end-labeling assay. RESULTS: Glutamine maintains the integrity of the mitochondria by reducing the cell permeability and cytochrome c levels and increasing the mitochondrial membrane potential. The cytochrome c level was significantly (pHospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2015-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/10201610.6061/clinics/2015(08)07Clinics; Vol. 70 No. 8 (2015); 569-576Clinics; v. 70 n. 8 (2015); 569-576Clinics; Vol. 70 Núm. 8 (2015); 569-5761980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/102016/100448Copyright (c) 2015 Clinicsinfo:eu-repo/semantics/openAccessSafi, Sher Zaman Batumalaie, Kalaivani Mansor, Marzida Chinna, Karuthan Mohan, Syam Karimian, Hamed Qvist, Rajes Ashraf, Muhammad Aqeel Yan, Garcie Ong Siok 2015-08-07T12:48:44Zoai:revistas.usp.br:article/102016Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2015-08-07T12:48:44Clinics - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Glutamine treatment attenuates hyperglycemia-induced mitochondrial stress and apoptosis in umbilical vein endothelial cells
title Glutamine treatment attenuates hyperglycemia-induced mitochondrial stress and apoptosis in umbilical vein endothelial cells
spellingShingle Glutamine treatment attenuates hyperglycemia-induced mitochondrial stress and apoptosis in umbilical vein endothelial cells
Safi, Sher Zaman
title_short Glutamine treatment attenuates hyperglycemia-induced mitochondrial stress and apoptosis in umbilical vein endothelial cells
title_full Glutamine treatment attenuates hyperglycemia-induced mitochondrial stress and apoptosis in umbilical vein endothelial cells
title_fullStr Glutamine treatment attenuates hyperglycemia-induced mitochondrial stress and apoptosis in umbilical vein endothelial cells
title_full_unstemmed Glutamine treatment attenuates hyperglycemia-induced mitochondrial stress and apoptosis in umbilical vein endothelial cells
title_sort Glutamine treatment attenuates hyperglycemia-induced mitochondrial stress and apoptosis in umbilical vein endothelial cells
author Safi, Sher Zaman
author_facet Safi, Sher Zaman
Batumalaie, Kalaivani
Mansor, Marzida
Chinna, Karuthan
Mohan, Syam
Karimian, Hamed
Qvist, Rajes
Ashraf, Muhammad Aqeel
Yan, Garcie Ong Siok
author_role author
author2 Batumalaie, Kalaivani
Mansor, Marzida
Chinna, Karuthan
Mohan, Syam
Karimian, Hamed
Qvist, Rajes
Ashraf, Muhammad Aqeel
Yan, Garcie Ong Siok
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Safi, Sher Zaman
Batumalaie, Kalaivani
Mansor, Marzida
Chinna, Karuthan
Mohan, Syam
Karimian, Hamed
Qvist, Rajes
Ashraf, Muhammad Aqeel
Yan, Garcie Ong Siok
description OBJECTIVE: The aim of this study was to determine the in vitro effect of glutamine and insulin on apoptosis, mitochondrial membrane potential, cell permeability, and inflammatory cytokines in hyperglycemic umbilical vein endothelial cells. MATERIALS AND METHODS: Human umbilical vein endothelial cells were grown and subjected to glutamine and insulin to examine the effects of these agents on the hyperglycemic state. Mitochondrial function and the production of inflammatory cytokines were assessed using fluorescence analysis and multiple cytotoxicity assays. Apoptosis was analyzed by the terminal deoxynucleotidyl transferase dUTP nick end-labeling assay. RESULTS: Glutamine maintains the integrity of the mitochondria by reducing the cell permeability and cytochrome c levels and increasing the mitochondrial membrane potential. The cytochrome c level was significantly (p
publishDate 2015
dc.date.none.fl_str_mv 2015-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/clinics/article/view/102016
10.6061/clinics/2015(08)07
url https://www.revistas.usp.br/clinics/article/view/102016
identifier_str_mv 10.6061/clinics/2015(08)07
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/clinics/article/view/102016/100448
dc.rights.driver.fl_str_mv Copyright (c) 2015 Clinics
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2015 Clinics
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
dc.source.none.fl_str_mv Clinics; Vol. 70 No. 8 (2015); 569-576
Clinics; v. 70 n. 8 (2015); 569-576
Clinics; Vol. 70 Núm. 8 (2015); 569-576
1980-5322
1807-5932
reponame:Clinics
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Clinics
collection Clinics
repository.name.fl_str_mv Clinics - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||clinics@hc.fm.usp.br
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