Establishment of the bacterial fecal community during the first month of life in Brazilian newborns
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Clinics |
Texto Completo: | https://www.revistas.usp.br/clinics/article/view/19676 |
Resumo: | OBJECTIVE: The establishment of the intestinal microbiota in newborns is a critical period with possible long-term consequences for human health. In this research, the development of the fecal microbiota of a group of exclusively breastfed neonates living in low socio-economic conditions in the city of São Paulo, Brazil, during the first month of life, was studied. METHODS: Fecal samples were collected from ten neonates on the second, seventh, and 30th days after birth. One of the neonates underwent antibiotic therapy. Molecular techniques were used for analysis; DNA was extracted from the samples, and 16S rRNA libraries were sequenced and phylogenetically analyzed after construction. A real-time polymerase chain reaction (PCR) was performed on the samples taken from the 30th day to amplify DNA from Bifidobacterium sp. RESULTS: The primary phylogenetic groups identified in the samples were Escherichia and Clostridium. Staphylococcus was identified at a low rate. Bifidobacterium sp. was detected in all of the samples collected on the 30th day. In the child who received antibiotics, a reduction in anaerobes and Escherichia, which was associated with an overgrowth of Klebsiella, was observed throughout the experimental period. CONCLUSION: The observed pattern of Escherichia predominance and reduced Staphylococcus colonization is in contrast with the patterns observed in neonates living in developed countries. |
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Clinics |
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Establishment of the bacterial fecal community during the first month of life in Brazilian newbornsMicrobiotaBreastfeeding16S rRNAEscherichiaClostridiumOBJECTIVE: The establishment of the intestinal microbiota in newborns is a critical period with possible long-term consequences for human health. In this research, the development of the fecal microbiota of a group of exclusively breastfed neonates living in low socio-economic conditions in the city of São Paulo, Brazil, during the first month of life, was studied. METHODS: Fecal samples were collected from ten neonates on the second, seventh, and 30th days after birth. One of the neonates underwent antibiotic therapy. Molecular techniques were used for analysis; DNA was extracted from the samples, and 16S rRNA libraries were sequenced and phylogenetically analyzed after construction. A real-time polymerase chain reaction (PCR) was performed on the samples taken from the 30th day to amplify DNA from Bifidobacterium sp. RESULTS: The primary phylogenetic groups identified in the samples were Escherichia and Clostridium. Staphylococcus was identified at a low rate. Bifidobacterium sp. was detected in all of the samples collected on the 30th day. In the child who received antibiotics, a reduction in anaerobes and Escherichia, which was associated with an overgrowth of Klebsiella, was observed throughout the experimental period. CONCLUSION: The observed pattern of Escherichia predominance and reduced Staphylococcus colonization is in contrast with the patterns observed in neonates living in developed countries.Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2012-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/1967610.6061/clinics/2012(02)05Clinics; Vol. 67 No. 2 (2012); 113-123Clinics; v. 67 n. 2 (2012); 113-123Clinics; Vol. 67 Núm. 2 (2012); 113-1231980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/19676/21740Brandt, KátiaTaddei, Carla R.Takagi, Elizabeth H.Oliveira, Fernanda F.Duarte, Rubens T. D.Irino, IsabelMartinez, Marina B.Carneiro-Sampaio, Magdainfo:eu-repo/semantics/openAccess2012-05-24T18:50:27Zoai:revistas.usp.br:article/19676Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2012-05-24T18:50:27Clinics - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Establishment of the bacterial fecal community during the first month of life in Brazilian newborns |
title |
Establishment of the bacterial fecal community during the first month of life in Brazilian newborns |
spellingShingle |
Establishment of the bacterial fecal community during the first month of life in Brazilian newborns Brandt, Kátia Microbiota Breastfeeding 16S rRNA Escherichia Clostridium |
title_short |
Establishment of the bacterial fecal community during the first month of life in Brazilian newborns |
title_full |
Establishment of the bacterial fecal community during the first month of life in Brazilian newborns |
title_fullStr |
Establishment of the bacterial fecal community during the first month of life in Brazilian newborns |
title_full_unstemmed |
Establishment of the bacterial fecal community during the first month of life in Brazilian newborns |
title_sort |
Establishment of the bacterial fecal community during the first month of life in Brazilian newborns |
author |
Brandt, Kátia |
author_facet |
Brandt, Kátia Taddei, Carla R. Takagi, Elizabeth H. Oliveira, Fernanda F. Duarte, Rubens T. D. Irino, Isabel Martinez, Marina B. Carneiro-Sampaio, Magda |
author_role |
author |
author2 |
Taddei, Carla R. Takagi, Elizabeth H. Oliveira, Fernanda F. Duarte, Rubens T. D. Irino, Isabel Martinez, Marina B. Carneiro-Sampaio, Magda |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Brandt, Kátia Taddei, Carla R. Takagi, Elizabeth H. Oliveira, Fernanda F. Duarte, Rubens T. D. Irino, Isabel Martinez, Marina B. Carneiro-Sampaio, Magda |
dc.subject.por.fl_str_mv |
Microbiota Breastfeeding 16S rRNA Escherichia Clostridium |
topic |
Microbiota Breastfeeding 16S rRNA Escherichia Clostridium |
description |
OBJECTIVE: The establishment of the intestinal microbiota in newborns is a critical period with possible long-term consequences for human health. In this research, the development of the fecal microbiota of a group of exclusively breastfed neonates living in low socio-economic conditions in the city of São Paulo, Brazil, during the first month of life, was studied. METHODS: Fecal samples were collected from ten neonates on the second, seventh, and 30th days after birth. One of the neonates underwent antibiotic therapy. Molecular techniques were used for analysis; DNA was extracted from the samples, and 16S rRNA libraries were sequenced and phylogenetically analyzed after construction. A real-time polymerase chain reaction (PCR) was performed on the samples taken from the 30th day to amplify DNA from Bifidobacterium sp. RESULTS: The primary phylogenetic groups identified in the samples were Escherichia and Clostridium. Staphylococcus was identified at a low rate. Bifidobacterium sp. was detected in all of the samples collected on the 30th day. In the child who received antibiotics, a reduction in anaerobes and Escherichia, which was associated with an overgrowth of Klebsiella, was observed throughout the experimental period. CONCLUSION: The observed pattern of Escherichia predominance and reduced Staphylococcus colonization is in contrast with the patterns observed in neonates living in developed countries. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/19676 10.6061/clinics/2012(02)05 |
url |
https://www.revistas.usp.br/clinics/article/view/19676 |
identifier_str_mv |
10.6061/clinics/2012(02)05 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/19676/21740 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
dc.source.none.fl_str_mv |
Clinics; Vol. 67 No. 2 (2012); 113-123 Clinics; v. 67 n. 2 (2012); 113-123 Clinics; Vol. 67 Núm. 2 (2012); 113-123 1980-5322 1807-5932 reponame:Clinics instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Clinics |
collection |
Clinics |
repository.name.fl_str_mv |
Clinics - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||clinics@hc.fm.usp.br |
_version_ |
1800222758234226688 |