Respiratory mechanics do not always mirror pulmonary histological changes in emphysema
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Clinics |
Texto Completo: | https://www.revistas.usp.br/clinics/article/view/19495 |
Resumo: | OBJECTIVE: To verify the accordance of functional and morphometric parameters during the development of emphysema. METHODS: BALB/c mice received a nasal drop of either papain or saline solution and were studied after 1, 3, 15, 28, and 40 days. Functional parameters, such as airway resistance, tissue damping, and tissue elastance, were analyzed. To evaluate the structural changes and possible mechanisms involved in this disease, we measured the mean linear intercept, the volume proportions of elastic and collagen fibers, the number of macrophages, the numbers of cells expressing metalloprotease 12 and 8-isoprostane in lung parenchyma. RESULTS: We only observed decreases in tissue elastance and tissue damping on the 28th day, with a concomitant increase in the mean linear intercept, indicating the presence of emphysema. However, only the mean linear intercept values remained increased until the 40th day. The volume proportion of collagen fibers was increased from the 15th day to the 40th day, whereas the volume proportion of elastic fibers was only increased on the 40th day. The number of macrophages increased beginning on the 1st day. The expression of metalloproteinase 12 was increased from the 3rd day until the 40th day. However, 8-isoprostane expression was only increased on the 1st and 3rd days. CONCLUSIONS: In this study, morphometric parameters were found to be more reliable for detecting the presence of emphysema than the functional parameters measured by respiratory mechanics. Further investigations are necessary to understand how the extracellular matrix remodeling observed in the lung parenchyma could be involved in this process. |
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Clinics |
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Respiratory mechanics do not always mirror pulmonary histological changes in emphysema EmphysemaAnimal modelMean linear interceptRespiratory mechanicsParenchyma remodeling OBJECTIVE: To verify the accordance of functional and morphometric parameters during the development of emphysema. METHODS: BALB/c mice received a nasal drop of either papain or saline solution and were studied after 1, 3, 15, 28, and 40 days. Functional parameters, such as airway resistance, tissue damping, and tissue elastance, were analyzed. To evaluate the structural changes and possible mechanisms involved in this disease, we measured the mean linear intercept, the volume proportions of elastic and collagen fibers, the number of macrophages, the numbers of cells expressing metalloprotease 12 and 8-isoprostane in lung parenchyma. RESULTS: We only observed decreases in tissue elastance and tissue damping on the 28th day, with a concomitant increase in the mean linear intercept, indicating the presence of emphysema. However, only the mean linear intercept values remained increased until the 40th day. The volume proportion of collagen fibers was increased from the 15th day to the 40th day, whereas the volume proportion of elastic fibers was only increased on the 40th day. The number of macrophages increased beginning on the 1st day. The expression of metalloproteinase 12 was increased from the 3rd day until the 40th day. However, 8-isoprostane expression was only increased on the 1st and 3rd days. CONCLUSIONS: In this study, morphometric parameters were found to be more reliable for detecting the presence of emphysema than the functional parameters measured by respiratory mechanics. Further investigations are necessary to understand how the extracellular matrix remodeling observed in the lung parenchyma could be involved in this process. Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2011-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/1949510.1590/S1807-59322011001000020Clinics; Vol. 66 No. 10 (2011); 1797-1803 Clinics; v. 66 n. 10 (2011); 1797-1803 Clinics; Vol. 66 Núm. 10 (2011); 1797-1803 1980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/19495/21558Anciaes, Adriana MartinsOlivo, Clarice RosaPrado, Carla MáximoKagohara, Keila H.Pinto, Tatiana da SilvaMoriya, Henrique T.Mauad, ThaisMartins, Mílton de ArrudaLopes, Fernanda Degobbi Tenorio Quirino dos Santosinfo:eu-repo/semantics/openAccess2012-05-23T16:43:58Zoai:revistas.usp.br:article/19495Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2012-05-23T16:43:58Clinics - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Respiratory mechanics do not always mirror pulmonary histological changes in emphysema |
title |
Respiratory mechanics do not always mirror pulmonary histological changes in emphysema |
spellingShingle |
Respiratory mechanics do not always mirror pulmonary histological changes in emphysema Anciaes, Adriana Martins Emphysema Animal model Mean linear intercept Respiratory mechanics Parenchyma remodeling |
title_short |
Respiratory mechanics do not always mirror pulmonary histological changes in emphysema |
title_full |
Respiratory mechanics do not always mirror pulmonary histological changes in emphysema |
title_fullStr |
Respiratory mechanics do not always mirror pulmonary histological changes in emphysema |
title_full_unstemmed |
Respiratory mechanics do not always mirror pulmonary histological changes in emphysema |
title_sort |
Respiratory mechanics do not always mirror pulmonary histological changes in emphysema |
author |
Anciaes, Adriana Martins |
author_facet |
Anciaes, Adriana Martins Olivo, Clarice Rosa Prado, Carla Máximo Kagohara, Keila H. Pinto, Tatiana da Silva Moriya, Henrique T. Mauad, Thais Martins, Mílton de Arruda Lopes, Fernanda Degobbi Tenorio Quirino dos Santos |
author_role |
author |
author2 |
Olivo, Clarice Rosa Prado, Carla Máximo Kagohara, Keila H. Pinto, Tatiana da Silva Moriya, Henrique T. Mauad, Thais Martins, Mílton de Arruda Lopes, Fernanda Degobbi Tenorio Quirino dos Santos |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Anciaes, Adriana Martins Olivo, Clarice Rosa Prado, Carla Máximo Kagohara, Keila H. Pinto, Tatiana da Silva Moriya, Henrique T. Mauad, Thais Martins, Mílton de Arruda Lopes, Fernanda Degobbi Tenorio Quirino dos Santos |
dc.subject.por.fl_str_mv |
Emphysema Animal model Mean linear intercept Respiratory mechanics Parenchyma remodeling |
topic |
Emphysema Animal model Mean linear intercept Respiratory mechanics Parenchyma remodeling |
description |
OBJECTIVE: To verify the accordance of functional and morphometric parameters during the development of emphysema. METHODS: BALB/c mice received a nasal drop of either papain or saline solution and were studied after 1, 3, 15, 28, and 40 days. Functional parameters, such as airway resistance, tissue damping, and tissue elastance, were analyzed. To evaluate the structural changes and possible mechanisms involved in this disease, we measured the mean linear intercept, the volume proportions of elastic and collagen fibers, the number of macrophages, the numbers of cells expressing metalloprotease 12 and 8-isoprostane in lung parenchyma. RESULTS: We only observed decreases in tissue elastance and tissue damping on the 28th day, with a concomitant increase in the mean linear intercept, indicating the presence of emphysema. However, only the mean linear intercept values remained increased until the 40th day. The volume proportion of collagen fibers was increased from the 15th day to the 40th day, whereas the volume proportion of elastic fibers was only increased on the 40th day. The number of macrophages increased beginning on the 1st day. The expression of metalloproteinase 12 was increased from the 3rd day until the 40th day. However, 8-isoprostane expression was only increased on the 1st and 3rd days. CONCLUSIONS: In this study, morphometric parameters were found to be more reliable for detecting the presence of emphysema than the functional parameters measured by respiratory mechanics. Further investigations are necessary to understand how the extracellular matrix remodeling observed in the lung parenchyma could be involved in this process. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/19495 10.1590/S1807-59322011001000020 |
url |
https://www.revistas.usp.br/clinics/article/view/19495 |
identifier_str_mv |
10.1590/S1807-59322011001000020 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/19495/21558 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
dc.source.none.fl_str_mv |
Clinics; Vol. 66 No. 10 (2011); 1797-1803 Clinics; v. 66 n. 10 (2011); 1797-1803 Clinics; Vol. 66 Núm. 10 (2011); 1797-1803 1980-5322 1807-5932 reponame:Clinics instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Clinics |
collection |
Clinics |
repository.name.fl_str_mv |
Clinics - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||clinics@hc.fm.usp.br |
_version_ |
1800222757357617152 |