Detection of pathogenic virus in fish fillets

• Autor(a) principal: Schmid, Ary Walter
• Data de Publicação: 1977
• Outros Autores: Stewien, Klaus E., Candeias, J. A. N.
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Revista de Saúde Pública
• Texto Completo: https://www.revistas.usp.br/rsp/article/view/22857
• topic: Vírus patogênicos humanos; Vírus^i1^scult; Viruses^i2^shuman pathoge; Virus cultivation

The purpose of this study was to develop a method for detection of virus in fish fillets. Samples (50g) of fish fillets were minced and shaken for 5 min with 200 ml of doubled distilled water (pH 7.3-7.5). The mixture was clarified by filtration. This food extract was subjected to filtration through a sterile sodium alginate membrane, which was thereafter dissolved in 2 ml of a 3.8% sodium citrate solution, and centrifuged at 10000 rev/min for 20 min, at 4°C. Penicillin, streptomycin and amphotericin B were added to the supernatant fraction. Virus isolations were done in primary rhesus (Macaca mulatta) monkey kidney cultures, HeLa cultures and newborn mice. Fifty one fish fillets were examined. The agents isolated were: 2 type 1 poliovirus, 2 type 3 poliovirus and one type B4 coxsackievirus, giving an isolation rate of 9.8%. Using the intratypic serodifferentiation for the characterization of the four polioviruses strains isolated from the fish fillets, only one was similar to the vaccine strain, and the three others were classified as non-vaccine like strains.