Reporter virus neutralization test evaluation for Dengue and Zika Virus diagnosis in flavivirus endemic area

Detalhes bibliográficos
Autor(a) principal: Nunes, Jannyce G. C
Data de Publicação: 2021
Outros Autores: Nunes, Bruno Tardelli Diniz, Shan, Chao, Moraes, Adriana Freitas, Silva, Tais R, Mendonça, Maria H. R. de, Chagas, Liliane L. das, Silva, Franco A. e, Azevedo, Raimunda do Socorro da Silva, Silva, Eliana Vieira Pinto da, Martins, Lívia Caricio, Chiang, Jannifer Oliveira, Casseb, Lívia Medeiros Neves, Henriques, Daniele Freitas, Vasconcelos, Pedro Fernando da Costa, Burbano, Rommel Mário Rodrígues, Shi, Pei-Yong, Medeiros, Daniele Barbosa de Almeida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/4394
Resumo: Reporter virus neutralization test (RVNT) has been used as an alternative to the more laborious and time-demanding conventional PRNT assay for both DENV and ZIKV. However, few studies have investigated how these techniques would perform in epidemic areas with the circulation of multiple flavivirus. Here, we evaluate the performance of ZIKV and DENV Rluc RVNT and ZIKV mCh RVNT assays in comparison to the conventional PRNT assay against patient sera collected before and during ZIKV outbreak in Brazil. These samples were categorized into groups based on (1) acute and convalescent samples according to the time of disease, and (2) laboratorial diagnostic results (DENV and ZIKV RT-PCR and IgM-capture ELISA). Our results showed that DENV Rluc assay presented 100% and 78.3% sensitivity and specificity, respectively, with 93.3% accuracy, a similar performance to the traditional PRNT. ZIKV RVNT90, on the other hand, showed much better ZIKV antibody detection performance (around nine-fold higher) when compared to PRNT, with 88% clinical sensitivity. Specificity values were on average 76.8%. Even with these results, however, ZIKV RVNT90 alone was not able to reach a final diagnostic conclusion for secondary infection in human samples due to flavivirus cross reaction. As such, in regions where the flavivirus differential diagnosis represents a challenge, we suggest the establishment of a RVNT panel including other flaviviruses circulating in the region, associated with the other serological techniques such as IgM ELISA and the investigation of seroconversion, in order to help define an accurate diagnostic conclusion using serology.
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spelling Nunes, Jannyce G. CNunes, Bruno Tardelli DinizShan, ChaoMoraes, Adriana FreitasSilva, Tais RMendonça, Maria H. R. deChagas, Liliane L. dasSilva, Franco A. eAzevedo, Raimunda do Socorro da SilvaSilva, Eliana Vieira Pinto daMartins, Lívia CaricioChiang, Jannifer OliveiraCasseb, Lívia Medeiros NevesHenriques, Daniele FreitasVasconcelos, Pedro Fernando da CostaBurbano, Rommel Mário RodríguesShi, Pei-YongMedeiros, Daniele Barbosa de Almeida2021-08-10T11:47:59Z2021-08-10T11:47:59Z2021NUNES, Jannyce G. C. et al. Reporter virus neutralization test evaluation for Dengue and Zika Virus diagnosis in flavivirus endemic area. Pathogens, v. 10, n. 7, p. 1-11, July 2021.2076-0817https://patua.iec.gov.br/handle/iec/439410.3390/pathogens10070840Reporter virus neutralization test (RVNT) has been used as an alternative to the more laborious and time-demanding conventional PRNT assay for both DENV and ZIKV. However, few studies have investigated how these techniques would perform in epidemic areas with the circulation of multiple flavivirus. Here, we evaluate the performance of ZIKV and DENV Rluc RVNT and ZIKV mCh RVNT assays in comparison to the conventional PRNT assay against patient sera collected before and during ZIKV outbreak in Brazil. These samples were categorized into groups based on (1) acute and convalescent samples according to the time of disease, and (2) laboratorial diagnostic results (DENV and ZIKV RT-PCR and IgM-capture ELISA). Our results showed that DENV Rluc assay presented 100% and 78.3% sensitivity and specificity, respectively, with 93.3% accuracy, a similar performance to the traditional PRNT. ZIKV RVNT90, on the other hand, showed much better ZIKV antibody detection performance (around nine-fold higher) when compared to PRNT, with 88% clinical sensitivity. Specificity values were on average 76.8%. Even with these results, however, ZIKV RVNT90 alone was not able to reach a final diagnostic conclusion for secondary infection in human samples due to flavivirus cross reaction. As such, in regions where the flavivirus differential diagnosis represents a challenge, we suggest the establishment of a RVNT panel including other flaviviruses circulating in the region, associated with the other serological techniques such as IgM ELISA and the investigation of seroconversion, in order to help define an accurate diagnostic conclusion using serology.Ministry of Health of Brazil and by the grants from CNPq (process 303999/2016-0 and 440405/2016-5) and CAPES (Zika fast track project). Pan American Health Organization SCON2016-01353, NIH grants AI142759, AI127744, and AI136126, and awards from the Kleberg Foundation, John S. Dunn Foundation, Amon G. Carter Foundation, Gilson Longenbaugh Foundation, and Summerfield Robert Foundation.University of Texas Medical Branch. Department of Biochemistry & Molecular Biology. Galveston, TX, USA / State University of Pará. Post Graduation Program in Parasitary Biology in the Amazon. Belém, PA, Brazil.University of Texas Medical Branch. Department of Biochemistry & Molecular Biology. Galveston, TX, USA / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.University of Texas Medical Branch. Department of Biochemistry & Molecular Biology. Galveston, TX, USA.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Pará State University. Science and Health Institute. Belém, PA, Brazil.Federal University of Pará. Biological Sciences Institute. Belém, PA, Brazil.University of Texas Medical Branch. Department of Biochemistry & Molecular Biology. Galveston, TX, USA .University of Texas Medical Branch. Department of Biochemistry & Molecular Biology. Galveston, TX, USA / State University of Pará. Post Graduation Program in Parasitary Biology in the Amazon. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. 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dc.title.pt_BR.fl_str_mv Reporter virus neutralization test evaluation for Dengue and Zika Virus diagnosis in flavivirus endemic area
title Reporter virus neutralization test evaluation for Dengue and Zika Virus diagnosis in flavivirus endemic area
spellingShingle Reporter virus neutralization test evaluation for Dengue and Zika Virus diagnosis in flavivirus endemic area
Nunes, Jannyce G. C
Flavivirus / isolamento & purificação
Testes de Neutralização / métodos
Vírus da Dengue / patogenicidade
Zika virus / patogenicidade
Vírus Repórter
title_short Reporter virus neutralization test evaluation for Dengue and Zika Virus diagnosis in flavivirus endemic area
title_full Reporter virus neutralization test evaluation for Dengue and Zika Virus diagnosis in flavivirus endemic area
title_fullStr Reporter virus neutralization test evaluation for Dengue and Zika Virus diagnosis in flavivirus endemic area
title_full_unstemmed Reporter virus neutralization test evaluation for Dengue and Zika Virus diagnosis in flavivirus endemic area
title_sort Reporter virus neutralization test evaluation for Dengue and Zika Virus diagnosis in flavivirus endemic area
author Nunes, Jannyce G. C
author_facet Nunes, Jannyce G. C
Nunes, Bruno Tardelli Diniz
Shan, Chao
Moraes, Adriana Freitas
Silva, Tais R
Mendonça, Maria H. R. de
Chagas, Liliane L. das
Silva, Franco A. e
Azevedo, Raimunda do Socorro da Silva
Silva, Eliana Vieira Pinto da
Martins, Lívia Caricio
Chiang, Jannifer Oliveira
Casseb, Lívia Medeiros Neves
Henriques, Daniele Freitas
Vasconcelos, Pedro Fernando da Costa
Burbano, Rommel Mário Rodrígues
Shi, Pei-Yong
Medeiros, Daniele Barbosa de Almeida
author_role author
author2 Nunes, Bruno Tardelli Diniz
Shan, Chao
Moraes, Adriana Freitas
Silva, Tais R
Mendonça, Maria H. R. de
Chagas, Liliane L. das
Silva, Franco A. e
Azevedo, Raimunda do Socorro da Silva
Silva, Eliana Vieira Pinto da
Martins, Lívia Caricio
Chiang, Jannifer Oliveira
Casseb, Lívia Medeiros Neves
Henriques, Daniele Freitas
Vasconcelos, Pedro Fernando da Costa
Burbano, Rommel Mário Rodrígues
Shi, Pei-Yong
Medeiros, Daniele Barbosa de Almeida
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Nunes, Jannyce G. C
Nunes, Bruno Tardelli Diniz
Shan, Chao
Moraes, Adriana Freitas
Silva, Tais R
Mendonça, Maria H. R. de
Chagas, Liliane L. das
Silva, Franco A. e
Azevedo, Raimunda do Socorro da Silva
Silva, Eliana Vieira Pinto da
Martins, Lívia Caricio
Chiang, Jannifer Oliveira
Casseb, Lívia Medeiros Neves
Henriques, Daniele Freitas
Vasconcelos, Pedro Fernando da Costa
Burbano, Rommel Mário Rodrígues
Shi, Pei-Yong
Medeiros, Daniele Barbosa de Almeida
dc.subject.decsPrimary.pt_BR.fl_str_mv Flavivirus / isolamento & purificação
Testes de Neutralização / métodos
Vírus da Dengue / patogenicidade
Zika virus / patogenicidade
Vírus Repórter
topic Flavivirus / isolamento & purificação
Testes de Neutralização / métodos
Vírus da Dengue / patogenicidade
Zika virus / patogenicidade
Vírus Repórter
description Reporter virus neutralization test (RVNT) has been used as an alternative to the more laborious and time-demanding conventional PRNT assay for both DENV and ZIKV. However, few studies have investigated how these techniques would perform in epidemic areas with the circulation of multiple flavivirus. Here, we evaluate the performance of ZIKV and DENV Rluc RVNT and ZIKV mCh RVNT assays in comparison to the conventional PRNT assay against patient sera collected before and during ZIKV outbreak in Brazil. These samples were categorized into groups based on (1) acute and convalescent samples according to the time of disease, and (2) laboratorial diagnostic results (DENV and ZIKV RT-PCR and IgM-capture ELISA). Our results showed that DENV Rluc assay presented 100% and 78.3% sensitivity and specificity, respectively, with 93.3% accuracy, a similar performance to the traditional PRNT. ZIKV RVNT90, on the other hand, showed much better ZIKV antibody detection performance (around nine-fold higher) when compared to PRNT, with 88% clinical sensitivity. Specificity values were on average 76.8%. Even with these results, however, ZIKV RVNT90 alone was not able to reach a final diagnostic conclusion for secondary infection in human samples due to flavivirus cross reaction. As such, in regions where the flavivirus differential diagnosis represents a challenge, we suggest the establishment of a RVNT panel including other flaviviruses circulating in the region, associated with the other serological techniques such as IgM ELISA and the investigation of seroconversion, in order to help define an accurate diagnostic conclusion using serology.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-08-10T11:47:59Z
dc.date.available.fl_str_mv 2021-08-10T11:47:59Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv NUNES, Jannyce G. C. et al. Reporter virus neutralization test evaluation for Dengue and Zika Virus diagnosis in flavivirus endemic area. Pathogens, v. 10, n. 7, p. 1-11, July 2021.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/4394
dc.identifier.issn.-.fl_str_mv 2076-0817
dc.identifier.doi.-.fl_str_mv 10.3390/pathogens10070840
identifier_str_mv NUNES, Jannyce G. C. et al. Reporter virus neutralization test evaluation for Dengue and Zika Virus diagnosis in flavivirus endemic area. Pathogens, v. 10, n. 7, p. 1-11, July 2021.
2076-0817
10.3390/pathogens10070840
url https://patua.iec.gov.br/handle/iec/4394
dc.language.iso.fl_str_mv eng
language eng
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dc.publisher.none.fl_str_mv MDPI
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