Reporter virus neutralization test evaluation for Dengue and Zika Virus diagnosis in flavivirus endemic area
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/4394 |
Resumo: | Reporter virus neutralization test (RVNT) has been used as an alternative to the more laborious and time-demanding conventional PRNT assay for both DENV and ZIKV. However, few studies have investigated how these techniques would perform in epidemic areas with the circulation of multiple flavivirus. Here, we evaluate the performance of ZIKV and DENV Rluc RVNT and ZIKV mCh RVNT assays in comparison to the conventional PRNT assay against patient sera collected before and during ZIKV outbreak in Brazil. These samples were categorized into groups based on (1) acute and convalescent samples according to the time of disease, and (2) laboratorial diagnostic results (DENV and ZIKV RT-PCR and IgM-capture ELISA). Our results showed that DENV Rluc assay presented 100% and 78.3% sensitivity and specificity, respectively, with 93.3% accuracy, a similar performance to the traditional PRNT. ZIKV RVNT90, on the other hand, showed much better ZIKV antibody detection performance (around nine-fold higher) when compared to PRNT, with 88% clinical sensitivity. Specificity values were on average 76.8%. Even with these results, however, ZIKV RVNT90 alone was not able to reach a final diagnostic conclusion for secondary infection in human samples due to flavivirus cross reaction. As such, in regions where the flavivirus differential diagnosis represents a challenge, we suggest the establishment of a RVNT panel including other flaviviruses circulating in the region, associated with the other serological techniques such as IgM ELISA and the investigation of seroconversion, in order to help define an accurate diagnostic conclusion using serology. |
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Nunes, Jannyce G. CNunes, Bruno Tardelli DinizShan, ChaoMoraes, Adriana FreitasSilva, Tais RMendonça, Maria H. R. deChagas, Liliane L. dasSilva, Franco A. eAzevedo, Raimunda do Socorro da SilvaSilva, Eliana Vieira Pinto daMartins, Lívia CaricioChiang, Jannifer OliveiraCasseb, Lívia Medeiros NevesHenriques, Daniele FreitasVasconcelos, Pedro Fernando da CostaBurbano, Rommel Mário RodríguesShi, Pei-YongMedeiros, Daniele Barbosa de Almeida2021-08-10T11:47:59Z2021-08-10T11:47:59Z2021NUNES, Jannyce G. C. et al. Reporter virus neutralization test evaluation for Dengue and Zika Virus diagnosis in flavivirus endemic area. Pathogens, v. 10, n. 7, p. 1-11, July 2021.2076-0817https://patua.iec.gov.br/handle/iec/439410.3390/pathogens10070840Reporter virus neutralization test (RVNT) has been used as an alternative to the more laborious and time-demanding conventional PRNT assay for both DENV and ZIKV. However, few studies have investigated how these techniques would perform in epidemic areas with the circulation of multiple flavivirus. Here, we evaluate the performance of ZIKV and DENV Rluc RVNT and ZIKV mCh RVNT assays in comparison to the conventional PRNT assay against patient sera collected before and during ZIKV outbreak in Brazil. These samples were categorized into groups based on (1) acute and convalescent samples according to the time of disease, and (2) laboratorial diagnostic results (DENV and ZIKV RT-PCR and IgM-capture ELISA). Our results showed that DENV Rluc assay presented 100% and 78.3% sensitivity and specificity, respectively, with 93.3% accuracy, a similar performance to the traditional PRNT. ZIKV RVNT90, on the other hand, showed much better ZIKV antibody detection performance (around nine-fold higher) when compared to PRNT, with 88% clinical sensitivity. Specificity values were on average 76.8%. Even with these results, however, ZIKV RVNT90 alone was not able to reach a final diagnostic conclusion for secondary infection in human samples due to flavivirus cross reaction. As such, in regions where the flavivirus differential diagnosis represents a challenge, we suggest the establishment of a RVNT panel including other flaviviruses circulating in the region, associated with the other serological techniques such as IgM ELISA and the investigation of seroconversion, in order to help define an accurate diagnostic conclusion using serology.Ministry of Health of Brazil and by the grants from CNPq (process 303999/2016-0 and 440405/2016-5) and CAPES (Zika fast track project). Pan American Health Organization SCON2016-01353, NIH grants AI142759, AI127744, and AI136126, and awards from the Kleberg Foundation, John S. Dunn Foundation, Amon G. Carter Foundation, Gilson Longenbaugh Foundation, and Summerfield Robert Foundation.University of Texas Medical Branch. Department of Biochemistry & Molecular Biology. Galveston, TX, USA / State University of Pará. Post Graduation Program in Parasitary Biology in the Amazon. Belém, PA, Brazil.University of Texas Medical Branch. Department of Biochemistry & Molecular Biology. Galveston, TX, USA / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.University of Texas Medical Branch. Department of Biochemistry & Molecular Biology. Galveston, TX, USA.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Pará State University. Science and Health Institute. Belém, PA, Brazil.Federal University of Pará. Biological Sciences Institute. Belém, PA, Brazil.University of Texas Medical Branch. Department of Biochemistry & Molecular Biology. Galveston, TX, USA .University of Texas Medical Branch. Department of Biochemistry & Molecular Biology. Galveston, TX, USA / State University of Pará. Post Graduation Program in Parasitary Biology in the Amazon. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. 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dc.title.pt_BR.fl_str_mv |
Reporter virus neutralization test evaluation for Dengue and Zika Virus diagnosis in flavivirus endemic area |
title |
Reporter virus neutralization test evaluation for Dengue and Zika Virus diagnosis in flavivirus endemic area |
spellingShingle |
Reporter virus neutralization test evaluation for Dengue and Zika Virus diagnosis in flavivirus endemic area Nunes, Jannyce G. C Flavivirus / isolamento & purificação Testes de Neutralização / métodos Vírus da Dengue / patogenicidade Zika virus / patogenicidade Vírus Repórter |
title_short |
Reporter virus neutralization test evaluation for Dengue and Zika Virus diagnosis in flavivirus endemic area |
title_full |
Reporter virus neutralization test evaluation for Dengue and Zika Virus diagnosis in flavivirus endemic area |
title_fullStr |
Reporter virus neutralization test evaluation for Dengue and Zika Virus diagnosis in flavivirus endemic area |
title_full_unstemmed |
Reporter virus neutralization test evaluation for Dengue and Zika Virus diagnosis in flavivirus endemic area |
title_sort |
Reporter virus neutralization test evaluation for Dengue and Zika Virus diagnosis in flavivirus endemic area |
author |
Nunes, Jannyce G. C |
author_facet |
Nunes, Jannyce G. C Nunes, Bruno Tardelli Diniz Shan, Chao Moraes, Adriana Freitas Silva, Tais R Mendonça, Maria H. R. de Chagas, Liliane L. das Silva, Franco A. e Azevedo, Raimunda do Socorro da Silva Silva, Eliana Vieira Pinto da Martins, Lívia Caricio Chiang, Jannifer Oliveira Casseb, Lívia Medeiros Neves Henriques, Daniele Freitas Vasconcelos, Pedro Fernando da Costa Burbano, Rommel Mário Rodrígues Shi, Pei-Yong Medeiros, Daniele Barbosa de Almeida |
author_role |
author |
author2 |
Nunes, Bruno Tardelli Diniz Shan, Chao Moraes, Adriana Freitas Silva, Tais R Mendonça, Maria H. R. de Chagas, Liliane L. das Silva, Franco A. e Azevedo, Raimunda do Socorro da Silva Silva, Eliana Vieira Pinto da Martins, Lívia Caricio Chiang, Jannifer Oliveira Casseb, Lívia Medeiros Neves Henriques, Daniele Freitas Vasconcelos, Pedro Fernando da Costa Burbano, Rommel Mário Rodrígues Shi, Pei-Yong Medeiros, Daniele Barbosa de Almeida |
author2_role |
author author author author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Nunes, Jannyce G. C Nunes, Bruno Tardelli Diniz Shan, Chao Moraes, Adriana Freitas Silva, Tais R Mendonça, Maria H. R. de Chagas, Liliane L. das Silva, Franco A. e Azevedo, Raimunda do Socorro da Silva Silva, Eliana Vieira Pinto da Martins, Lívia Caricio Chiang, Jannifer Oliveira Casseb, Lívia Medeiros Neves Henriques, Daniele Freitas Vasconcelos, Pedro Fernando da Costa Burbano, Rommel Mário Rodrígues Shi, Pei-Yong Medeiros, Daniele Barbosa de Almeida |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Flavivirus / isolamento & purificação Testes de Neutralização / métodos Vírus da Dengue / patogenicidade Zika virus / patogenicidade Vírus Repórter |
topic |
Flavivirus / isolamento & purificação Testes de Neutralização / métodos Vírus da Dengue / patogenicidade Zika virus / patogenicidade Vírus Repórter |
description |
Reporter virus neutralization test (RVNT) has been used as an alternative to the more laborious and time-demanding conventional PRNT assay for both DENV and ZIKV. However, few studies have investigated how these techniques would perform in epidemic areas with the circulation of multiple flavivirus. Here, we evaluate the performance of ZIKV and DENV Rluc RVNT and ZIKV mCh RVNT assays in comparison to the conventional PRNT assay against patient sera collected before and during ZIKV outbreak in Brazil. These samples were categorized into groups based on (1) acute and convalescent samples according to the time of disease, and (2) laboratorial diagnostic results (DENV and ZIKV RT-PCR and IgM-capture ELISA). Our results showed that DENV Rluc assay presented 100% and 78.3% sensitivity and specificity, respectively, with 93.3% accuracy, a similar performance to the traditional PRNT. ZIKV RVNT90, on the other hand, showed much better ZIKV antibody detection performance (around nine-fold higher) when compared to PRNT, with 88% clinical sensitivity. Specificity values were on average 76.8%. Even with these results, however, ZIKV RVNT90 alone was not able to reach a final diagnostic conclusion for secondary infection in human samples due to flavivirus cross reaction. As such, in regions where the flavivirus differential diagnosis represents a challenge, we suggest the establishment of a RVNT panel including other flaviviruses circulating in the region, associated with the other serological techniques such as IgM ELISA and the investigation of seroconversion, in order to help define an accurate diagnostic conclusion using serology. |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-08-10T11:47:59Z |
dc.date.available.fl_str_mv |
2021-08-10T11:47:59Z |
dc.date.issued.fl_str_mv |
2021 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
NUNES, Jannyce G. C. et al. Reporter virus neutralization test evaluation for Dengue and Zika Virus diagnosis in flavivirus endemic area. Pathogens, v. 10, n. 7, p. 1-11, July 2021. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/4394 |
dc.identifier.issn.-.fl_str_mv |
2076-0817 |
dc.identifier.doi.-.fl_str_mv |
10.3390/pathogens10070840 |
identifier_str_mv |
NUNES, Jannyce G. C. et al. Reporter virus neutralization test evaluation for Dengue and Zika Virus diagnosis in flavivirus endemic area. Pathogens, v. 10, n. 7, p. 1-11, July 2021. 2076-0817 10.3390/pathogens10070840 |
url |
https://patua.iec.gov.br/handle/iec/4394 |
dc.language.iso.fl_str_mv |
eng |
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eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
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