Detection of pathogenic virus in fish fillets
Autor(a) principal: | |
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Data de Publicação: | 1977 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista de Saúde Pública |
Texto Completo: | https://www.revistas.usp.br/rsp/article/view/22857 |
Resumo: | The purpose of this study was to develop a method for detection of virus in fish fillets. Samples (50g) of fish fillets were minced and shaken for 5 min with 200 ml of doubled distilled water (pH 7.3-7.5). The mixture was clarified by filtration. This food extract was subjected to filtration through a sterile sodium alginate membrane, which was thereafter dissolved in 2 ml of a 3.8% sodium citrate solution, and centrifuged at 10000 rev/min for 20 min, at 4°C. Penicillin, streptomycin and amphotericin B were added to the supernatant fraction. Virus isolations were done in primary rhesus (Macaca mulatta) monkey kidney cultures, HeLa cultures and newborn mice. Fifty one fish fillets were examined. The agents isolated were: 2 type 1 poliovirus, 2 type 3 poliovirus and one type B4 coxsackievirus, giving an isolation rate of 9.8%. Using the intratypic serodifferentiation for the characterization of the four polioviruses strains isolated from the fish fillets, only one was similar to the vaccine strain, and the three others were classified as non-vaccine like strains. |
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Detection of pathogenic virus in fish fillets Evidenciação de vírus patogênicos humanos em filés de peixe Vírus patogênicos humanosVírus^i1^scultViruses^i2^shuman pathogeVirus cultivation The purpose of this study was to develop a method for detection of virus in fish fillets. Samples (50g) of fish fillets were minced and shaken for 5 min with 200 ml of doubled distilled water (pH 7.3-7.5). The mixture was clarified by filtration. This food extract was subjected to filtration through a sterile sodium alginate membrane, which was thereafter dissolved in 2 ml of a 3.8% sodium citrate solution, and centrifuged at 10000 rev/min for 20 min, at 4°C. Penicillin, streptomycin and amphotericin B were added to the supernatant fraction. Virus isolations were done in primary rhesus (Macaca mulatta) monkey kidney cultures, HeLa cultures and newborn mice. Fifty one fish fillets were examined. The agents isolated were: 2 type 1 poliovirus, 2 type 3 poliovirus and one type B4 coxsackievirus, giving an isolation rate of 9.8%. Using the intratypic serodifferentiation for the characterization of the four polioviruses strains isolated from the fish fillets, only one was similar to the vaccine strain, and the three others were classified as non-vaccine like strains. Foi desenvolvido método para a evidenciação de vírus em filés de peixe. Amostras de 50 gramas foram submetidas à agitação mecânica durante 5 min em 200 ml de água bidestilada esterilizada, em pH 7,3-7,5. As substâncias em suspensão foram removidas mediante uma filtração de clarificação. A suspensão clarificada foi em seguida submetida à ultrafiltração através de membrana de alginato de sódio, sendo esta posteriormente dissolvida em 2 ml de citrato de sódio a 3,8%. A suspensão obtida foi, a seguir, centrifugada a 10.000 rpm durante 20 min, a 4°C, adicionando-se ao sobrenadante penicilina, estreptomicina e anfotericina B. O isolamento de vírus foi feito em culturas primárias de células de rim de macaco rhesus, células HeLa e por inoculação em camundongos recém-nascidos. A identificação sorológica levou aos seguintes resultados: de 51 filés de peixe foram isoladas duas estirpes de poliovírus tipo 1, duas de poliovírus tipo 3 e uma de coxsackievírus B4, o que corresponde a uma percentagem de positividade de 9,8%*. A identificação intratípica das quatro estirpes de poliovírus isoladas revelou ser uma delas semelhante e as demais diferentes das estirpes vacinais. Universidade de São Paulo. Faculdade de Saúde Pública1977-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rsp/article/view/2285710.1590/S0034-89101977000300012Revista de Saúde Pública; Vol. 11 No. 3 (1977); 405-409 Revista de Saúde Pública; Vol. 11 Núm. 3 (1977); 405-409 Revista de Saúde Pública; v. 11 n. 3 (1977); 405-409 1518-87870034-8910reponame:Revista de Saúde Públicainstname:Universidade de São Paulo (USP)instacron:USPporhttps://www.revistas.usp.br/rsp/article/view/22857/24881Copyright (c) 2017 Revista de Saúde Públicainfo:eu-repo/semantics/openAccessSchmid, Ary WalterStewien, Klaus E.Candeias, J. A. N.2012-05-28T14:01:41Zoai:revistas.usp.br:article/22857Revistahttps://www.revistas.usp.br/rsp/indexONGhttps://www.revistas.usp.br/rsp/oairevsp@org.usp.br||revsp1@usp.br1518-87870034-8910opendoar:2012-05-28T14:01:41Revista de Saúde Pública - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Detection of pathogenic virus in fish fillets Evidenciação de vírus patogênicos humanos em filés de peixe |
title |
Detection of pathogenic virus in fish fillets |
spellingShingle |
Detection of pathogenic virus in fish fillets Schmid, Ary Walter Vírus patogênicos humanos Vírus^i1^scult Viruses^i2^shuman pathoge Virus cultivation |
title_short |
Detection of pathogenic virus in fish fillets |
title_full |
Detection of pathogenic virus in fish fillets |
title_fullStr |
Detection of pathogenic virus in fish fillets |
title_full_unstemmed |
Detection of pathogenic virus in fish fillets |
title_sort |
Detection of pathogenic virus in fish fillets |
author |
Schmid, Ary Walter |
author_facet |
Schmid, Ary Walter Stewien, Klaus E. Candeias, J. A. N. |
author_role |
author |
author2 |
Stewien, Klaus E. Candeias, J. A. N. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Schmid, Ary Walter Stewien, Klaus E. Candeias, J. A. N. |
dc.subject.por.fl_str_mv |
Vírus patogênicos humanos Vírus^i1^scult Viruses^i2^shuman pathoge Virus cultivation |
topic |
Vírus patogênicos humanos Vírus^i1^scult Viruses^i2^shuman pathoge Virus cultivation |
description |
The purpose of this study was to develop a method for detection of virus in fish fillets. Samples (50g) of fish fillets were minced and shaken for 5 min with 200 ml of doubled distilled water (pH 7.3-7.5). The mixture was clarified by filtration. This food extract was subjected to filtration through a sterile sodium alginate membrane, which was thereafter dissolved in 2 ml of a 3.8% sodium citrate solution, and centrifuged at 10000 rev/min for 20 min, at 4°C. Penicillin, streptomycin and amphotericin B were added to the supernatant fraction. Virus isolations were done in primary rhesus (Macaca mulatta) monkey kidney cultures, HeLa cultures and newborn mice. Fifty one fish fillets were examined. The agents isolated were: 2 type 1 poliovirus, 2 type 3 poliovirus and one type B4 coxsackievirus, giving an isolation rate of 9.8%. Using the intratypic serodifferentiation for the characterization of the four polioviruses strains isolated from the fish fillets, only one was similar to the vaccine strain, and the three others were classified as non-vaccine like strains. |
publishDate |
1977 |
dc.date.none.fl_str_mv |
1977-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rsp/article/view/22857 10.1590/S0034-89101977000300012 |
url |
https://www.revistas.usp.br/rsp/article/view/22857 |
identifier_str_mv |
10.1590/S0034-89101977000300012 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rsp/article/view/22857/24881 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2017 Revista de Saúde Pública info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2017 Revista de Saúde Pública |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Saúde Pública |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Saúde Pública |
dc.source.none.fl_str_mv |
Revista de Saúde Pública; Vol. 11 No. 3 (1977); 405-409 Revista de Saúde Pública; Vol. 11 Núm. 3 (1977); 405-409 Revista de Saúde Pública; v. 11 n. 3 (1977); 405-409 1518-8787 0034-8910 reponame:Revista de Saúde Pública instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Revista de Saúde Pública |
collection |
Revista de Saúde Pública |
repository.name.fl_str_mv |
Revista de Saúde Pública - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
revsp@org.usp.br||revsp1@usp.br |
_version_ |
1800221769734291456 |