Development and validation of a method for the determination of folic acid in different pharmaceutical formulations using derivative spectrophotometry
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Pharmaceutical Sciences |
Texto Completo: | https://www.revistas.usp.br/bjps/article/view/128428 |
Resumo: | Folic acid is a B complex water-soluble vitamin that is essential to humans, and its deficiency can cause problems including congenital malformations in the fetus as well as heart disease. Most countries affected by diseases associated with a lack of folic acid now supplement foods with the vitamin. There is therefore a need for the development of new analytical procedures able to determine folic acid present in different matrices. This work describes the development of zero order and first order derivative spectrophotometric methods for the determination of folic acid in different pharmaceutical formulations, using 0.1 mol L-1 NaOH as solvent. The methods are shown to be simple, selective, and robust. Good linearity was achieved, with correction coefficients ≥0.9996 and limits of detection and quantification ranging from 0.64 to 0.75 and from 1.80 to 2.85 mg L-1, respectively. Recoveries of 98-104% were obtained in accuracy tests, and precision (as RSD) was between 0.2 and 4.8%. The methods can be used in routine analyses for quality control purposes, offering an alternative to the procedures already reported in the literature. |
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Brazilian Journal of Pharmaceutical Sciences |
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Development and validation of a method for the determination of folic acid in different pharmaceutical formulations using derivative spectrophotometry Folic acid is a B complex water-soluble vitamin that is essential to humans, and its deficiency can cause problems including congenital malformations in the fetus as well as heart disease. Most countries affected by diseases associated with a lack of folic acid now supplement foods with the vitamin. There is therefore a need for the development of new analytical procedures able to determine folic acid present in different matrices. This work describes the development of zero order and first order derivative spectrophotometric methods for the determination of folic acid in different pharmaceutical formulations, using 0.1 mol L-1 NaOH as solvent. The methods are shown to be simple, selective, and robust. Good linearity was achieved, with correction coefficients ≥0.9996 and limits of detection and quantification ranging from 0.64 to 0.75 and from 1.80 to 2.85 mg L-1, respectively. Recoveries of 98-104% were obtained in accuracy tests, and precision (as RSD) was between 0.2 and 4.8%. The methods can be used in routine analyses for quality control purposes, offering an alternative to the procedures already reported in the literature. Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2016-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/12842810.1590/s1984-82502016000400019Brazilian Journal of Pharmaceutical Sciences; Vol. 52 Núm. 4 (2016); 741-750Brazilian Journal of Pharmaceutical Sciences; v. 52 n. 4 (2016); 741-750Brazilian Journal of Pharmaceutical Sciences; Vol. 52 No. 4 (2016); 741-7502175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/128428/125280Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso)info:eu-repo/semantics/openAccessRibeiro, Marcos Vinícius de MouraMelo, Ingrid da SilvaLopes, Francisco das Chagas da CostaMoita, Graziella Ciaramella2017-03-16T18:09:44Zoai:revistas.usp.br:article/128428Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2017-03-16T18:09:44Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Development and validation of a method for the determination of folic acid in different pharmaceutical formulations using derivative spectrophotometry |
title |
Development and validation of a method for the determination of folic acid in different pharmaceutical formulations using derivative spectrophotometry |
spellingShingle |
Development and validation of a method for the determination of folic acid in different pharmaceutical formulations using derivative spectrophotometry Ribeiro, Marcos Vinícius de Moura |
title_short |
Development and validation of a method for the determination of folic acid in different pharmaceutical formulations using derivative spectrophotometry |
title_full |
Development and validation of a method for the determination of folic acid in different pharmaceutical formulations using derivative spectrophotometry |
title_fullStr |
Development and validation of a method for the determination of folic acid in different pharmaceutical formulations using derivative spectrophotometry |
title_full_unstemmed |
Development and validation of a method for the determination of folic acid in different pharmaceutical formulations using derivative spectrophotometry |
title_sort |
Development and validation of a method for the determination of folic acid in different pharmaceutical formulations using derivative spectrophotometry |
author |
Ribeiro, Marcos Vinícius de Moura |
author_facet |
Ribeiro, Marcos Vinícius de Moura Melo, Ingrid da Silva Lopes, Francisco das Chagas da Costa Moita, Graziella Ciaramella |
author_role |
author |
author2 |
Melo, Ingrid da Silva Lopes, Francisco das Chagas da Costa Moita, Graziella Ciaramella |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Ribeiro, Marcos Vinícius de Moura Melo, Ingrid da Silva Lopes, Francisco das Chagas da Costa Moita, Graziella Ciaramella |
description |
Folic acid is a B complex water-soluble vitamin that is essential to humans, and its deficiency can cause problems including congenital malformations in the fetus as well as heart disease. Most countries affected by diseases associated with a lack of folic acid now supplement foods with the vitamin. There is therefore a need for the development of new analytical procedures able to determine folic acid present in different matrices. This work describes the development of zero order and first order derivative spectrophotometric methods for the determination of folic acid in different pharmaceutical formulations, using 0.1 mol L-1 NaOH as solvent. The methods are shown to be simple, selective, and robust. Good linearity was achieved, with correction coefficients ≥0.9996 and limits of detection and quantification ranging from 0.64 to 0.75 and from 1.80 to 2.85 mg L-1, respectively. Recoveries of 98-104% were obtained in accuracy tests, and precision (as RSD) was between 0.2 and 4.8%. The methods can be used in routine analyses for quality control purposes, offering an alternative to the procedures already reported in the literature. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/128428 10.1590/s1984-82502016000400019 |
url |
https://www.revistas.usp.br/bjps/article/view/128428 |
identifier_str_mv |
10.1590/s1984-82502016000400019 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/128428/125280 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso) info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso) |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
dc.source.none.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences; Vol. 52 Núm. 4 (2016); 741-750 Brazilian Journal of Pharmaceutical Sciences; v. 52 n. 4 (2016); 741-750 Brazilian Journal of Pharmaceutical Sciences; Vol. 52 No. 4 (2016); 741-750 2175-9790 1984-8250 reponame:Brazilian Journal of Pharmaceutical Sciences instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Pharmaceutical Sciences |
collection |
Brazilian Journal of Pharmaceutical Sciences |
repository.name.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
bjps@usp.br||elizabeth.igne@gmail.com |
_version_ |
1800222912880312320 |