Importance of the agar-media in the evaluation of bacteriocin activity against the same test-microorganisms
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Pharmaceutical Sciences |
Texto Completo: | https://www.revistas.usp.br/bjps/article/view/146811 |
Resumo: | Bacteriocins are peptides produced by various species of bacteria, especially lactic acid bacteria, which exhibit a large spectrum of action against spoilage bacteria and foodborne pathogens. Successful application of techniques for quantitative or qualitative bacteriocin determination relies not only on the sensitivity of the test-microorganisms, but also on the agar-medium employed. Cell free supernatants are routinely used to preliminary screen for antimicrobial activity of bacteria by means of the agar well diffusion method, but the supernatant may also include other molecules (such as medium components and/or intracellular compounds) accidentally released during cell free supernatant preparation, which may interfere with the assay. Reproducibility of bacteriocin activity against the same test-microorganisms is an important factor to be considered. Unfortunately, no specific information about bioassays standardization to determine bacteriocin activity is available in the literature. In this work, growth inhibition by means of the agar well diffusion assays were carried out on different agar-media showing a strong dependence on the agar-medium used, indicating that the inhibitory effects could also depend on the diffusion of exudates that are included in the cell-free supernatant. The results presented in this communication show that selection of the agar-medium is crucial for the bioassay response. |
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Brazilian Journal of Pharmaceutical Sciences |
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Importance of the agar-media in the evaluation of bacteriocin activity against the same test-microorganismsBacteriocinGrowth inhibitionSolid mediumAgar well diffusion assay Bacteriocins are peptides produced by various species of bacteria, especially lactic acid bacteria, which exhibit a large spectrum of action against spoilage bacteria and foodborne pathogens. Successful application of techniques for quantitative or qualitative bacteriocin determination relies not only on the sensitivity of the test-microorganisms, but also on the agar-medium employed. Cell free supernatants are routinely used to preliminary screen for antimicrobial activity of bacteria by means of the agar well diffusion method, but the supernatant may also include other molecules (such as medium components and/or intracellular compounds) accidentally released during cell free supernatant preparation, which may interfere with the assay. Reproducibility of bacteriocin activity against the same test-microorganisms is an important factor to be considered. Unfortunately, no specific information about bioassays standardization to determine bacteriocin activity is available in the literature. In this work, growth inhibition by means of the agar well diffusion assays were carried out on different agar-media showing a strong dependence on the agar-medium used, indicating that the inhibitory effects could also depend on the diffusion of exudates that are included in the cell-free supernatant. The results presented in this communication show that selection of the agar-medium is crucial for the bioassay response.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2018-06-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/14681110.1590/s2175-97902018000117533Brazilian Journal of Pharmaceutical Sciences; Vol. 54 Núm. 1 (2018); e17533Brazilian Journal of Pharmaceutical Sciences; v. 54 n. 1 (2018); e17533Brazilian Journal of Pharmaceutical Sciences; Vol. 54 No. 1 (2018); e175332175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/146811/140340Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso)info:eu-repo/semantics/openAccessAzevedo, Pamela Oliveira de Souza deMolinari, FrancescoOliveira, Ricardo Pinheiro de Souza2018-06-07T16:31:56Zoai:revistas.usp.br:article/146811Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2018-06-07T16:31:56Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Importance of the agar-media in the evaluation of bacteriocin activity against the same test-microorganisms |
title |
Importance of the agar-media in the evaluation of bacteriocin activity against the same test-microorganisms |
spellingShingle |
Importance of the agar-media in the evaluation of bacteriocin activity against the same test-microorganisms Azevedo, Pamela Oliveira de Souza de Bacteriocin Growth inhibition Solid medium Agar well diffusion assay |
title_short |
Importance of the agar-media in the evaluation of bacteriocin activity against the same test-microorganisms |
title_full |
Importance of the agar-media in the evaluation of bacteriocin activity against the same test-microorganisms |
title_fullStr |
Importance of the agar-media in the evaluation of bacteriocin activity against the same test-microorganisms |
title_full_unstemmed |
Importance of the agar-media in the evaluation of bacteriocin activity against the same test-microorganisms |
title_sort |
Importance of the agar-media in the evaluation of bacteriocin activity against the same test-microorganisms |
author |
Azevedo, Pamela Oliveira de Souza de |
author_facet |
Azevedo, Pamela Oliveira de Souza de Molinari, Francesco Oliveira, Ricardo Pinheiro de Souza |
author_role |
author |
author2 |
Molinari, Francesco Oliveira, Ricardo Pinheiro de Souza |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Azevedo, Pamela Oliveira de Souza de Molinari, Francesco Oliveira, Ricardo Pinheiro de Souza |
dc.subject.por.fl_str_mv |
Bacteriocin Growth inhibition Solid medium Agar well diffusion assay |
topic |
Bacteriocin Growth inhibition Solid medium Agar well diffusion assay |
description |
Bacteriocins are peptides produced by various species of bacteria, especially lactic acid bacteria, which exhibit a large spectrum of action against spoilage bacteria and foodborne pathogens. Successful application of techniques for quantitative or qualitative bacteriocin determination relies not only on the sensitivity of the test-microorganisms, but also on the agar-medium employed. Cell free supernatants are routinely used to preliminary screen for antimicrobial activity of bacteria by means of the agar well diffusion method, but the supernatant may also include other molecules (such as medium components and/or intracellular compounds) accidentally released during cell free supernatant preparation, which may interfere with the assay. Reproducibility of bacteriocin activity against the same test-microorganisms is an important factor to be considered. Unfortunately, no specific information about bioassays standardization to determine bacteriocin activity is available in the literature. In this work, growth inhibition by means of the agar well diffusion assays were carried out on different agar-media showing a strong dependence on the agar-medium used, indicating that the inhibitory effects could also depend on the diffusion of exudates that are included in the cell-free supernatant. The results presented in this communication show that selection of the agar-medium is crucial for the bioassay response. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-06-07 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/146811 10.1590/s2175-97902018000117533 |
url |
https://www.revistas.usp.br/bjps/article/view/146811 |
identifier_str_mv |
10.1590/s2175-97902018000117533 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/146811/140340 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso) info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso) |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
dc.source.none.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences; Vol. 54 Núm. 1 (2018); e17533 Brazilian Journal of Pharmaceutical Sciences; v. 54 n. 1 (2018); e17533 Brazilian Journal of Pharmaceutical Sciences; Vol. 54 No. 1 (2018); e17533 2175-9790 1984-8250 reponame:Brazilian Journal of Pharmaceutical Sciences instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Pharmaceutical Sciences |
collection |
Brazilian Journal of Pharmaceutical Sciences |
repository.name.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
bjps@usp.br||elizabeth.igne@gmail.com |
_version_ |
1800222913336442880 |