An innovative, simple, fast, and less toxic high-performance liquid chromatographic method for determination of prednisone in capsules

• Autor(a) principal: Silva, Pedro Henrique Reis da
• Data de Publicação: 2017
• Outros Autores: Enéas, Paula Cristina Rezende, Fernandes, Christian, Pianetti, Gerson Antônio
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Journal of Pharmaceutical Sciences
• Texto Completo: https://www.revistas.usp.br/bjps/article/view/134169
• topic: Prednisone; Capsules; High performance liquid chromatography/validation; Linearity assessment.

Prednisone is an anti-inflammatory steroid drug widely used in clinical practice. However, no high-performance liquid chromatographic (HPLC) method has been described in the literature for the determination of prednisone in capsules until now. Thus, an HPLC method was developed using a C18 (250x4.0, 5 µm) column, with methanol:water (70:30) as mobile phase at a flow rate of 1 mL/min and detection at 240 nm. The developed method was validated following current Brazilian legislation. Additionally, linearity was assessed by evaluating the assumptions of normality, homoscedasticity, and independency of residuals, and the fit to the linear model. The method showed linearity (r2>;0.99) over the range of 14.0-26.0 µg/mL, selectivity, precision (RSD