An innovative, simple, fast, and less toxic high-performance liquid chromatographic method for determination of prednisone in capsules

Detalhes bibliográficos
Autor(a) principal: Silva, Pedro Henrique Reis da
Data de Publicação: 2017
Outros Autores: Enéas, Paula Cristina Rezende, Fernandes, Christian, Pianetti, Gerson Antônio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/27155
Resumo: Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Produtos Farmaceuticos. Belo Horizonte, MG, Brazil
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spelling Silva, Pedro Henrique Reis daEnéas, Paula Cristina RezendeFernandes, ChristianPianetti, Gerson Antônio2018-06-27T14:53:12Z2018-06-27T14:53:12Z2017SILVA, Pedro Henrique Reis da et al. An innovative, simple, fast, and less toxic high-performance liquid chromatographic method for determination of prednisone in capsules. Braz. J. Pharm. Sci, v. 53, n. 2, e16064, 2017.2175-9790https://www.arca.fiocruz.br/handle/icict/2715510.1590/s2175-97902017000216064engUniversidade de São PauloPrednisonacapsulasCromatografia líquida de alta eficiência / validaçãoAvaliação da linearidadePrednisoneCapsulesHigh performance liquid chromatography/validationLinearity assessmentAn innovative, simple, fast, and less toxic high-performance liquid chromatographic method for determination of prednisone in capsulesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Produtos Farmaceuticos. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Produtos Farmaceuticos. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Produtos Farmaceuticos. Belo Horizonte, MG, BrazilPrednisone is an anti-inflammatory steroid drug widely used in clinical practice. However, no high-performance liquid chromatographic (HPLC) method has been described in the literature for the determination of prednisone in capsules until now. Thus, an HPLC method was developed using a C18 (250x4.0, 5 µm) column, with methanol:water (70:30) as mobile phase at a flow rate of 1 mL/min and detection at 240 nm. The developed method was validated following current Brazilian legislation. Additionally, linearity was assessed by evaluating the assumptions of normality, homoscedasticity, and independency of residuals, and the fit to the linear model. The method showed linearity (r2>0.99) over the range of 14.0-26.0 µg/mL, selectivity, precision (RSD<2.0%), robustness, and accuracy (average recovery of 100.05%). The chromatographic procedure was applied for assay and uniformity content determination of three different batches of prednisone capsules, showing to be suitable for their quality control.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/27155/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALAn innovative, simple, fast,.pdfAn innovative, simple, fast,.pdfapplication/pdf401694https://www.arca.fiocruz.br/bitstream/icict/27155/2/An%20innovative%2c%20simple%2c%20fast%2c.pdfb36672d4b399a1db6f0f3dc5a99dfd6dMD52TEXTAn innovative, simple, fast,.pdf.txtAn innovative, simple, fast,.pdf.txtExtracted texttext/plain24251https://www.arca.fiocruz.br/bitstream/icict/27155/3/An%20innovative%2c%20simple%2c%20fast%2c.pdf.txt594e71ded5592d6252b1cfd3da94a0eeMD53icict/271552019-06-19 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dc.title.pt_BR.fl_str_mv An innovative, simple, fast, and less toxic high-performance liquid chromatographic method for determination of prednisone in capsules
title An innovative, simple, fast, and less toxic high-performance liquid chromatographic method for determination of prednisone in capsules
spellingShingle An innovative, simple, fast, and less toxic high-performance liquid chromatographic method for determination of prednisone in capsules
Silva, Pedro Henrique Reis da
Prednisona
capsulas
Cromatografia líquida de alta eficiência / validação
Avaliação da linearidade
Prednisone
Capsules
High performance liquid chromatography/validation
Linearity assessment
title_short An innovative, simple, fast, and less toxic high-performance liquid chromatographic method for determination of prednisone in capsules
title_full An innovative, simple, fast, and less toxic high-performance liquid chromatographic method for determination of prednisone in capsules
title_fullStr An innovative, simple, fast, and less toxic high-performance liquid chromatographic method for determination of prednisone in capsules
title_full_unstemmed An innovative, simple, fast, and less toxic high-performance liquid chromatographic method for determination of prednisone in capsules
title_sort An innovative, simple, fast, and less toxic high-performance liquid chromatographic method for determination of prednisone in capsules
author Silva, Pedro Henrique Reis da
author_facet Silva, Pedro Henrique Reis da
Enéas, Paula Cristina Rezende
Fernandes, Christian
Pianetti, Gerson Antônio
author_role author
author2 Enéas, Paula Cristina Rezende
Fernandes, Christian
Pianetti, Gerson Antônio
author2_role author
author
author
dc.contributor.author.fl_str_mv Silva, Pedro Henrique Reis da
Enéas, Paula Cristina Rezende
Fernandes, Christian
Pianetti, Gerson Antônio
dc.subject.other.pt_BR.fl_str_mv Prednisona
capsulas
Cromatografia líquida de alta eficiência / validação
Avaliação da linearidade
topic Prednisona
capsulas
Cromatografia líquida de alta eficiência / validação
Avaliação da linearidade
Prednisone
Capsules
High performance liquid chromatography/validation
Linearity assessment
dc.subject.en.pt_BR.fl_str_mv Prednisone
Capsules
High performance liquid chromatography/validation
Linearity assessment
description Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Produtos Farmaceuticos. Belo Horizonte, MG, Brazil
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-06-27T14:53:12Z
dc.date.available.fl_str_mv 2018-06-27T14:53:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, Pedro Henrique Reis da et al. An innovative, simple, fast, and less toxic high-performance liquid chromatographic method for determination of prednisone in capsules. Braz. J. Pharm. Sci, v. 53, n. 2, e16064, 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/27155
dc.identifier.issn.pt_BR.fl_str_mv 2175-9790
dc.identifier.doi.none.fl_str_mv 10.1590/s2175-97902017000216064
identifier_str_mv SILVA, Pedro Henrique Reis da et al. An innovative, simple, fast, and less toxic high-performance liquid chromatographic method for determination of prednisone in capsules. Braz. J. Pharm. Sci, v. 53, n. 2, e16064, 2017.
2175-9790
10.1590/s2175-97902017000216064
url https://www.arca.fiocruz.br/handle/icict/27155
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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