Nanostructured lipid carriers as a novel tool to deliver sclareol: physicochemical characterisation and evaluation in human cancer cell lines

Detalhes bibliográficos
Autor(a) principal: Silva Marques Borges, Gabriel
Data de Publicação: 2022
Outros Autores: Dias Moura Prazeres, Pedro Henrique, Malachias de Souza, Ângelo, Yoshida, Maria Irene, Carneiro Vilela, José Mario, Teixeira Maciel e Silva, Aline, Silva Oliveira, Mariana, Assis Gomes, Dawidson, Spangler Andrade, Margareth, de Souza-Fagundes, Elaine Maria, Miranda Ferreira , Lucas Antônio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Pharmaceutical Sciences
Texto Completo: https://www.revistas.usp.br/bjps/article/view/200989
Resumo: Sclareol (SC) is arousing great interest due to its cytostatic and cytotoxic activities in several cancer cell lines. However, its hydrophobicity is a limiting factor for its in vivo administration. One way to solve this problem is through nanoencapsulation. Therefore, solid lipid nanoparticles (SLN-SC) and nanostructured lipid carriers (NLC-SC) loaded with SC were produced and compared regarding their physicochemical properties. NLC-SC showed better SC encapsulation than SLN-SC and was chosen to be compared with free SC in human cancer cell lines (MDA-MB-231 and HCT-116). Free SC had slightly higher cytotoxicity than NLC-SC and produced subdiploid DNA content in both cell lines. On the other hand, NLC-SC led to subdiploid content in MDA-MB-231 cells and G2/M checkpoint arrest in HCT-116 cells. These findings suggest that SC encapsulation in NLC is a way to allow the in vivo administration of SC and might alter its biological properties.
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spelling Nanostructured lipid carriers as a novel tool to deliver sclareol: physicochemical characterisation and evaluation in human cancer cell linesCancer. Sclareol. Solid lipid nanoparticles. Nanostructured lipid carriers. Small-angle X-ray scattering.Sclareol (SC) is arousing great interest due to its cytostatic and cytotoxic activities in several cancer cell lines. However, its hydrophobicity is a limiting factor for its in vivo administration. One way to solve this problem is through nanoencapsulation. Therefore, solid lipid nanoparticles (SLN-SC) and nanostructured lipid carriers (NLC-SC) loaded with SC were produced and compared regarding their physicochemical properties. NLC-SC showed better SC encapsulation than SLN-SC and was chosen to be compared with free SC in human cancer cell lines (MDA-MB-231 and HCT-116). Free SC had slightly higher cytotoxicity than NLC-SC and produced subdiploid DNA content in both cell lines. On the other hand, NLC-SC led to subdiploid content in MDA-MB-231 cells and G2/M checkpoint arrest in HCT-116 cells. These findings suggest that SC encapsulation in NLC is a way to allow the in vivo administration of SC and might alter its biological properties.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2022-11-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/20098910.1590/s2175-97902020000418497Brazilian Journal of Pharmaceutical Sciences; Vol. 57 (2021)Brazilian Journal of Pharmaceutical Sciences; v. 57 (2021)Brazilian Journal of Pharmaceutical Sciences; Vol. 57 (2021)2175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/200989/185172https://www.revistas.usp.br/bjps/article/view/200989/185173Copyright (c) 2022 Brazilian Journal of Pharmaceutical Scienceshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSilva Marques Borges, GabrielDias Moura Prazeres, Pedro Henrique Malachias de Souza, ÂngeloYoshida, Maria IreneCarneiro Vilela, José MarioTeixeira Maciel e Silva, AlineSilva Oliveira, MarianaAssis Gomes, DawidsonSpangler Andrade, Margareth de Souza-Fagundes, Elaine MariaMiranda Ferreira , Lucas Antônio2022-11-09T17:30:27Zoai:revistas.usp.br:article/200989Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2022-11-09T17:30:27Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Nanostructured lipid carriers as a novel tool to deliver sclareol: physicochemical characterisation and evaluation in human cancer cell lines
title Nanostructured lipid carriers as a novel tool to deliver sclareol: physicochemical characterisation and evaluation in human cancer cell lines
spellingShingle Nanostructured lipid carriers as a novel tool to deliver sclareol: physicochemical characterisation and evaluation in human cancer cell lines
Silva Marques Borges, Gabriel
Cancer. Sclareol. Solid lipid nanoparticles. Nanostructured lipid carriers. Small-angle X-ray scattering.
title_short Nanostructured lipid carriers as a novel tool to deliver sclareol: physicochemical characterisation and evaluation in human cancer cell lines
title_full Nanostructured lipid carriers as a novel tool to deliver sclareol: physicochemical characterisation and evaluation in human cancer cell lines
title_fullStr Nanostructured lipid carriers as a novel tool to deliver sclareol: physicochemical characterisation and evaluation in human cancer cell lines
title_full_unstemmed Nanostructured lipid carriers as a novel tool to deliver sclareol: physicochemical characterisation and evaluation in human cancer cell lines
title_sort Nanostructured lipid carriers as a novel tool to deliver sclareol: physicochemical characterisation and evaluation in human cancer cell lines
author Silva Marques Borges, Gabriel
author_facet Silva Marques Borges, Gabriel
Dias Moura Prazeres, Pedro Henrique
Malachias de Souza, Ângelo
Yoshida, Maria Irene
Carneiro Vilela, José Mario
Teixeira Maciel e Silva, Aline
Silva Oliveira, Mariana
Assis Gomes, Dawidson
Spangler Andrade, Margareth
de Souza-Fagundes, Elaine Maria
Miranda Ferreira , Lucas Antônio
author_role author
author2 Dias Moura Prazeres, Pedro Henrique
Malachias de Souza, Ângelo
Yoshida, Maria Irene
Carneiro Vilela, José Mario
Teixeira Maciel e Silva, Aline
Silva Oliveira, Mariana
Assis Gomes, Dawidson
Spangler Andrade, Margareth
de Souza-Fagundes, Elaine Maria
Miranda Ferreira , Lucas Antônio
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva Marques Borges, Gabriel
Dias Moura Prazeres, Pedro Henrique
Malachias de Souza, Ângelo
Yoshida, Maria Irene
Carneiro Vilela, José Mario
Teixeira Maciel e Silva, Aline
Silva Oliveira, Mariana
Assis Gomes, Dawidson
Spangler Andrade, Margareth
de Souza-Fagundes, Elaine Maria
Miranda Ferreira , Lucas Antônio
dc.subject.por.fl_str_mv Cancer. Sclareol. Solid lipid nanoparticles. Nanostructured lipid carriers. Small-angle X-ray scattering.
topic Cancer. Sclareol. Solid lipid nanoparticles. Nanostructured lipid carriers. Small-angle X-ray scattering.
description Sclareol (SC) is arousing great interest due to its cytostatic and cytotoxic activities in several cancer cell lines. However, its hydrophobicity is a limiting factor for its in vivo administration. One way to solve this problem is through nanoencapsulation. Therefore, solid lipid nanoparticles (SLN-SC) and nanostructured lipid carriers (NLC-SC) loaded with SC were produced and compared regarding their physicochemical properties. NLC-SC showed better SC encapsulation than SLN-SC and was chosen to be compared with free SC in human cancer cell lines (MDA-MB-231 and HCT-116). Free SC had slightly higher cytotoxicity than NLC-SC and produced subdiploid DNA content in both cell lines. On the other hand, NLC-SC led to subdiploid content in MDA-MB-231 cells and G2/M checkpoint arrest in HCT-116 cells. These findings suggest that SC encapsulation in NLC is a way to allow the in vivo administration of SC and might alter its biological properties.
publishDate 2022
dc.date.none.fl_str_mv 2022-11-09
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjps/article/view/200989
10.1590/s2175-97902020000418497
url https://www.revistas.usp.br/bjps/article/view/200989
identifier_str_mv 10.1590/s2175-97902020000418497
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjps/article/view/200989/185172
https://www.revistas.usp.br/bjps/article/view/200989/185173
dc.rights.driver.fl_str_mv Copyright (c) 2022 Brazilian Journal of Pharmaceutical Sciences
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2022 Brazilian Journal of Pharmaceutical Sciences
https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Ciências Farmacêuticas
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Ciências Farmacêuticas
dc.source.none.fl_str_mv Brazilian Journal of Pharmaceutical Sciences; Vol. 57 (2021)
Brazilian Journal of Pharmaceutical Sciences; v. 57 (2021)
Brazilian Journal of Pharmaceutical Sciences; Vol. 57 (2021)
2175-9790
1984-8250
reponame:Brazilian Journal of Pharmaceutical Sciences
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Pharmaceutical Sciences
collection Brazilian Journal of Pharmaceutical Sciences
repository.name.fl_str_mv Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)
repository.mail.fl_str_mv bjps@usp.br||elizabeth.igne@gmail.com
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