Performance of IFAT, ELISA, direct parasitological examination and PCR on lymph node aspirates for canine visceral leishmaniasis diagnosis

Detalhes bibliográficos
Autor(a) principal: Camargo, J. B. [UNESP]
Data de Publicação: 2010
Outros Autores: Langoni, Hélio [UNESP], Troncarelli, M. Z. [UNESP], Machado, J. G. [UNESP], Lucheis, Simone Baldini [UNESP], Padovani, C. R. [UNESP]
Tipo de documento: Artigo de conferência
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1678-91992010000300007
http://hdl.handle.net/11449/13899
Resumo: Canine visceral leishmaniasis (CVL) is endemic in numerous Brazilian regions. The greatest difficulty in controlling the disease is the diagnostic limitation. In the present study, the most common tests employed for visceral leishmaniasis diagnosis were compared: immunofluorescence antibody test (IFAT), immunoenzymatic assay (ELISA), direct parasitological examination and polymerase chain reaction (PCR). Samples of lymph node aspirates and blood were collected from 100 dogs that lived in an endemic area (Bauru city, São Paulo state) and from 100 negative controls from a non-endemic area (Botucatu city, São Paulo state). Specificity of both IFAT and PCR was 100% whereas ELISA was 99%. Sensitivities were 97.77, 93.33 and 91.11% respectively for IFAT, ELISA and PCR.
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spelling Performance of IFAT, ELISA, direct parasitological examination and PCR on lymph node aspirates for canine visceral leishmaniasis diagnosisvisceral leishmaniasisdogdiagnosisCanine visceral leishmaniasis (CVL) is endemic in numerous Brazilian regions. The greatest difficulty in controlling the disease is the diagnostic limitation. In the present study, the most common tests employed for visceral leishmaniasis diagnosis were compared: immunofluorescence antibody test (IFAT), immunoenzymatic assay (ELISA), direct parasitological examination and polymerase chain reaction (PCR). Samples of lymph node aspirates and blood were collected from 100 dogs that lived in an endemic area (Bauru city, São Paulo state) and from 100 negative controls from a non-endemic area (Botucatu city, São Paulo state). Specificity of both IFAT and PCR was 100% whereas ELISA was 99%. Sensitivities were 97.77, 93.33 and 91.11% respectively for IFAT, ELISA and PCR.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)UNESP Univ Estadual Paulista, São Paulo State Univ, Sch Vet Med & Anim Husb, Dept Vet Hyg & Publ Hlth, Botucatu, SP, BrazilAPTA SAA, São Paulo Agcy Agribusiness Technol, Bauru, SP, BrazilUNESP Univ Estadual Paulista, São Paulo State Univ, Dept Biostat, Botucatu Biosci Inst, Botucatu, SP, BrazilUNESP Univ Estadual Paulista, São Paulo State Univ, Sch Vet Med & Anim Husb, Dept Vet Hyg & Publ Hlth, Botucatu, SP, BrazilUNESP Univ Estadual Paulista, São Paulo State Univ, Dept Biostat, Botucatu Biosci Inst, Botucatu, SP, BrazilUniversidade Estadual Paulista (Unesp), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Camargo, J. B. [UNESP]Langoni, Hélio [UNESP]Troncarelli, M. Z. [UNESP]Machado, J. G. [UNESP]Lucheis, Simone Baldini [UNESP]Padovani, C. R. [UNESP]2014-05-20T13:40:00Z2014-05-20T13:40:00Z2010-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObject414-420application/pdfhttp://dx.doi.org/10.1590/S1678-91992010000300007Journal of Venomous Animals and Toxins Including Tropical Diseases. Botucatu: Cevap-unesp, v. 16, n. 3, p. 414-420, 2010.1678-9199http://hdl.handle.net/11449/13899S1678-91992010000300007WOS:000281692600007S1678-91992010000300007-en.pdf8727897080522289Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins Including Tropical Diseases1.7820,573info:eu-repo/semantics/openAccess2023-11-19T06:14:08Zoai:repositorio.unesp.br:11449/13899Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:09:24.376867Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Performance of IFAT, ELISA, direct parasitological examination and PCR on lymph node aspirates for canine visceral leishmaniasis diagnosis
title Performance of IFAT, ELISA, direct parasitological examination and PCR on lymph node aspirates for canine visceral leishmaniasis diagnosis
spellingShingle Performance of IFAT, ELISA, direct parasitological examination and PCR on lymph node aspirates for canine visceral leishmaniasis diagnosis
Camargo, J. B. [UNESP]
visceral leishmaniasis
dog
diagnosis
title_short Performance of IFAT, ELISA, direct parasitological examination and PCR on lymph node aspirates for canine visceral leishmaniasis diagnosis
title_full Performance of IFAT, ELISA, direct parasitological examination and PCR on lymph node aspirates for canine visceral leishmaniasis diagnosis
title_fullStr Performance of IFAT, ELISA, direct parasitological examination and PCR on lymph node aspirates for canine visceral leishmaniasis diagnosis
title_full_unstemmed Performance of IFAT, ELISA, direct parasitological examination and PCR on lymph node aspirates for canine visceral leishmaniasis diagnosis
title_sort Performance of IFAT, ELISA, direct parasitological examination and PCR on lymph node aspirates for canine visceral leishmaniasis diagnosis
author Camargo, J. B. [UNESP]
author_facet Camargo, J. B. [UNESP]
Langoni, Hélio [UNESP]
Troncarelli, M. Z. [UNESP]
Machado, J. G. [UNESP]
Lucheis, Simone Baldini [UNESP]
Padovani, C. R. [UNESP]
author_role author
author2 Langoni, Hélio [UNESP]
Troncarelli, M. Z. [UNESP]
Machado, J. G. [UNESP]
Lucheis, Simone Baldini [UNESP]
Padovani, C. R. [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Camargo, J. B. [UNESP]
Langoni, Hélio [UNESP]
Troncarelli, M. Z. [UNESP]
Machado, J. G. [UNESP]
Lucheis, Simone Baldini [UNESP]
Padovani, C. R. [UNESP]
dc.subject.por.fl_str_mv visceral leishmaniasis
dog
diagnosis
topic visceral leishmaniasis
dog
diagnosis
description Canine visceral leishmaniasis (CVL) is endemic in numerous Brazilian regions. The greatest difficulty in controlling the disease is the diagnostic limitation. In the present study, the most common tests employed for visceral leishmaniasis diagnosis were compared: immunofluorescence antibody test (IFAT), immunoenzymatic assay (ELISA), direct parasitological examination and polymerase chain reaction (PCR). Samples of lymph node aspirates and blood were collected from 100 dogs that lived in an endemic area (Bauru city, São Paulo state) and from 100 negative controls from a non-endemic area (Botucatu city, São Paulo state). Specificity of both IFAT and PCR was 100% whereas ELISA was 99%. Sensitivities were 97.77, 93.33 and 91.11% respectively for IFAT, ELISA and PCR.
publishDate 2010
dc.date.none.fl_str_mv 2010-01-01
2014-05-20T13:40:00Z
2014-05-20T13:40:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/conferenceObject
format conferenceObject
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1678-91992010000300007
Journal of Venomous Animals and Toxins Including Tropical Diseases. Botucatu: Cevap-unesp, v. 16, n. 3, p. 414-420, 2010.
1678-9199
http://hdl.handle.net/11449/13899
S1678-91992010000300007
WOS:000281692600007
S1678-91992010000300007-en.pdf
8727897080522289
url http://dx.doi.org/10.1590/S1678-91992010000300007
http://hdl.handle.net/11449/13899
identifier_str_mv Journal of Venomous Animals and Toxins Including Tropical Diseases. Botucatu: Cevap-unesp, v. 16, n. 3, p. 414-420, 2010.
1678-9199
S1678-91992010000300007
WOS:000281692600007
S1678-91992010000300007-en.pdf
8727897080522289
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Venomous Animals and Toxins Including Tropical Diseases
1.782
0,573
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 414-420
application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP)
publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP)
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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