Early responses of Eucalyptus grandis during rust infection
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Tipo de documento: | Tese |
Idioma: | eng |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da USP |
Texto Completo: | http://www.teses.usp.br/teses/disponiveis/11/11137/tde-30042020-170013/ |
Resumo: | Eucalyptus grandis (E. grandis) is the main tree species used to produce paper and pulp in Brazil, which is the second-largest global producer. Rust disease is among the most harmful conditions affecting Eucalyptus plantations, and it is caused by the biotrophic fungus Austropuccinia psidii (A. psidii), which can interfere with annual tree development and lead to productivity losses. In this context, we aimed to study the molecular events involved in the early response of E. grandis to rust infection. To accomplish these aims, contrasting plant genotypes derived from a half-sibling progeny of E. grandis were either inoculated with A. psidii or subjected to control conditions to evaluate their physiological, metabolomic and proteomic responses 24 hours after inoculation (hai). Our results showed that responses of the genotypes against A. psidii diverged at 12 hai, when the rust-resistant genotype detected the fungus and initiated both oxidative burst and flavonoid production as an effective defense against the pathogen. Simultaneously, the immune system of the rustsusceptible genotype was unable to induce the production of ROS (reactive oxygen species) and hypersensitive response was suppressed. Thus, our findings enabled us to understand some of the key molecular mechanisms that underly the interactions between E. grandis and A. psidii, identify key molecules for further study of the disease pathology, and important features for enhancing molecular breeding efforts. |
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Early responses of Eucalyptus grandis during rust infectionRespostas iniciais de Eucalyptus grandis durante a infecção por ferrugemAustropuccinia psidiiAustropuccinia psidiiFisiologia vegetalMetabolômicaMetabolomicsPlant physiologyProteômicaProteomicsEucalyptus grandis (E. grandis) is the main tree species used to produce paper and pulp in Brazil, which is the second-largest global producer. Rust disease is among the most harmful conditions affecting Eucalyptus plantations, and it is caused by the biotrophic fungus Austropuccinia psidii (A. psidii), which can interfere with annual tree development and lead to productivity losses. In this context, we aimed to study the molecular events involved in the early response of E. grandis to rust infection. To accomplish these aims, contrasting plant genotypes derived from a half-sibling progeny of E. grandis were either inoculated with A. psidii or subjected to control conditions to evaluate their physiological, metabolomic and proteomic responses 24 hours after inoculation (hai). Our results showed that responses of the genotypes against A. psidii diverged at 12 hai, when the rust-resistant genotype detected the fungus and initiated both oxidative burst and flavonoid production as an effective defense against the pathogen. Simultaneously, the immune system of the rustsusceptible genotype was unable to induce the production of ROS (reactive oxygen species) and hypersensitive response was suppressed. Thus, our findings enabled us to understand some of the key molecular mechanisms that underly the interactions between E. grandis and A. psidii, identify key molecules for further study of the disease pathology, and important features for enhancing molecular breeding efforts.Eucalyptus grandis (E. grandis) é a principal espécie florestal utilizada para produção de papel e celulose no Brasil, o segundo maior produtor do mundo. Entre as condições de estresse que podem afetar as plantações de eucalipto, a ferrugem causada pelo fungo biotrófico Austropuccinia psidii (A. psidii) pode interferir no desenvolvimento anual das árvores e conduzir a perdas de produtividade. Neste contexto, o objetivo deste trabalho foi estudar os eventos moleculares envolvidos nas respostas iniciais de E. grandis durante a infecção por ferrugem. Para isso, plantas de dois genótipos contrastantes, derivados de uma progênie de meios-irmãos de E. grandis, foram tratadas em condições de inoculação e controle para avaliar suas respostas fisiológicas, metabólicas e do proteoma durante 24 horas após a inoculação (hai). Os resultados mostraram que os genótipos iniciam uma divergência na resposta à infecção por A. psidii 12 hai, quando o genótipo resistente já detectou o fungo para induzir explosões oxidativas e produção de flavonoides como uma resposta efetiva contra o patógeno, ao mesmo tempo que o genótipo suscetível apresenta subversão do sistema imune na indução de detoxificação de EROS (espécies reativas de oxigênio) e supressão das respostas de hipersensibilidade. Portanto, este estudo possibilitou a compreensão de alguns dos mecanismos moleculares que ocorrem na interação entre E. grandis e A. psidii e a identificação de moléculas-chave que podem possivelmente auxiliar estudos futuros sobre a patologia da doença e, também, o melhoramento molecular.Biblioteca Digitais de Teses e Dissertações da USPLabate, Carlos AlbertoSekiya, Alline2020-03-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttp://www.teses.usp.br/teses/disponiveis/11/11137/tde-30042020-170013/reponame:Biblioteca Digital de Teses e Dissertações da USPinstname:Universidade de São Paulo (USP)instacron:USPLiberar o conteúdo para acesso público.info:eu-repo/semantics/openAccesseng2022-04-30T12:59:49Zoai:teses.usp.br:tde-30042020-170013Biblioteca Digital de Teses e Dissertaçõeshttp://www.teses.usp.br/PUBhttp://www.teses.usp.br/cgi-bin/mtd2br.plvirginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.bropendoar:27212022-04-30T12:59:49Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Early responses of Eucalyptus grandis during rust infection Respostas iniciais de Eucalyptus grandis durante a infecção por ferrugem |
title |
Early responses of Eucalyptus grandis during rust infection |
spellingShingle |
Early responses of Eucalyptus grandis during rust infection Sekiya, Alline Austropuccinia psidii Austropuccinia psidii Fisiologia vegetal Metabolômica Metabolomics Plant physiology Proteômica Proteomics |
title_short |
Early responses of Eucalyptus grandis during rust infection |
title_full |
Early responses of Eucalyptus grandis during rust infection |
title_fullStr |
Early responses of Eucalyptus grandis during rust infection |
title_full_unstemmed |
Early responses of Eucalyptus grandis during rust infection |
title_sort |
Early responses of Eucalyptus grandis during rust infection |
author |
Sekiya, Alline |
author_facet |
Sekiya, Alline |
author_role |
author |
dc.contributor.none.fl_str_mv |
Labate, Carlos Alberto |
dc.contributor.author.fl_str_mv |
Sekiya, Alline |
dc.subject.por.fl_str_mv |
Austropuccinia psidii Austropuccinia psidii Fisiologia vegetal Metabolômica Metabolomics Plant physiology Proteômica Proteomics |
topic |
Austropuccinia psidii Austropuccinia psidii Fisiologia vegetal Metabolômica Metabolomics Plant physiology Proteômica Proteomics |
description |
Eucalyptus grandis (E. grandis) is the main tree species used to produce paper and pulp in Brazil, which is the second-largest global producer. Rust disease is among the most harmful conditions affecting Eucalyptus plantations, and it is caused by the biotrophic fungus Austropuccinia psidii (A. psidii), which can interfere with annual tree development and lead to productivity losses. In this context, we aimed to study the molecular events involved in the early response of E. grandis to rust infection. To accomplish these aims, contrasting plant genotypes derived from a half-sibling progeny of E. grandis were either inoculated with A. psidii or subjected to control conditions to evaluate their physiological, metabolomic and proteomic responses 24 hours after inoculation (hai). Our results showed that responses of the genotypes against A. psidii diverged at 12 hai, when the rust-resistant genotype detected the fungus and initiated both oxidative burst and flavonoid production as an effective defense against the pathogen. Simultaneously, the immune system of the rustsusceptible genotype was unable to induce the production of ROS (reactive oxygen species) and hypersensitive response was suppressed. Thus, our findings enabled us to understand some of the key molecular mechanisms that underly the interactions between E. grandis and A. psidii, identify key molecules for further study of the disease pathology, and important features for enhancing molecular breeding efforts. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-03-12 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.teses.usp.br/teses/disponiveis/11/11137/tde-30042020-170013/ |
url |
http://www.teses.usp.br/teses/disponiveis/11/11137/tde-30042020-170013/ |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
|
dc.rights.driver.fl_str_mv |
Liberar o conteúdo para acesso público. info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Liberar o conteúdo para acesso público. |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
|
dc.publisher.none.fl_str_mv |
Biblioteca Digitais de Teses e Dissertações da USP |
publisher.none.fl_str_mv |
Biblioteca Digitais de Teses e Dissertações da USP |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da USP instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Biblioteca Digital de Teses e Dissertações da USP |
collection |
Biblioteca Digital de Teses e Dissertações da USP |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
virginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.br |
_version_ |
1809091197966221312 |