Cutaneous attributes of chlorogenic acid, apigenin, kaempferol and naringenin established by HPLC-TBARS-EVSC protocol and Laser Doppler flowmetry

Detalhes bibliográficos
Autor(a) principal: Ruscinc, Nadia
Data de Publicação: 2024
Tipo de documento: Tese
Idioma: eng
Título da fonte: Biblioteca Digital de Teses e Dissertações da USP
Texto Completo: https://www.teses.usp.br/teses/disponiveis/9/9139/tde-22072024-100427/
Resumo: The integumentary system, a vital organ for organisms, constitutes a multifaceted barrier against pathogens and environmental factors, crucial for maintaining homeostasis. Skin, composed of epidermis, dermis, and hypodermis, plays a pivotal role in preserving life. Intrinsic and extrinsic factors can accelerate skin aging and compromise its homeostatic functions. Solar radiation, particularly ultraviolet (UV) radiation, poses a significant risk for skin cancer and accelerates photoaging. This study explores the in vitro safety and protective effects of polyphenols (chlorogenic acid, apigenin, kaempferol, and naringenin) against UV-induced lipid peroxidation using innovative methodologies, including HET-CAM assay, HPLC-TBARS-EVSC, and laser Doppler flowmetry. The HET-CAM assay confirmed non-irritating profiles for the polyphenols. HPLC-TBARS-EVSC revealed that only naringenin exhibited a significant reduction in lipid peroxidation, while others showed pro-oxidant tendencies. Laser Doppler flowmetry demonstrated anti-inflammatory potential, with naringenin displaying superior efficacy in reducing inflammatory response induced by methyl nicotinate. This comprehensive investigation underscores the diverse protective roles of polyphenols in skin health, emphasizing naringenin\'s notable anti-radical and antiinflammatory properties.
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spelling Cutaneous attributes of chlorogenic acid, apigenin, kaempferol and naringenin established by HPLC-TBARS-EVSC protocol and Laser Doppler flowmetryAtributos cutâneos do ácido clorogênico, apigenina, kaempferol e naringenina estabelecidos pelo protocolo HPLC-TBARS-EVSC e fluxometria Laser DopplerEstresss oxidativoLipid peroxidationOxidative stressPeroxidação lipídicaPolifenoisPolyphenolsTBARSTBARSThe integumentary system, a vital organ for organisms, constitutes a multifaceted barrier against pathogens and environmental factors, crucial for maintaining homeostasis. Skin, composed of epidermis, dermis, and hypodermis, plays a pivotal role in preserving life. Intrinsic and extrinsic factors can accelerate skin aging and compromise its homeostatic functions. Solar radiation, particularly ultraviolet (UV) radiation, poses a significant risk for skin cancer and accelerates photoaging. This study explores the in vitro safety and protective effects of polyphenols (chlorogenic acid, apigenin, kaempferol, and naringenin) against UV-induced lipid peroxidation using innovative methodologies, including HET-CAM assay, HPLC-TBARS-EVSC, and laser Doppler flowmetry. The HET-CAM assay confirmed non-irritating profiles for the polyphenols. HPLC-TBARS-EVSC revealed that only naringenin exhibited a significant reduction in lipid peroxidation, while others showed pro-oxidant tendencies. Laser Doppler flowmetry demonstrated anti-inflammatory potential, with naringenin displaying superior efficacy in reducing inflammatory response induced by methyl nicotinate. This comprehensive investigation underscores the diverse protective roles of polyphenols in skin health, emphasizing naringenin\'s notable anti-radical and antiinflammatory properties.O sistema tegumentar, órgão vital para os organismos, constitui uma barreira multifacetada contra patógenos e fatores ambientais, crucial para a manutenção da homeostase. A pele, composta por epiderme, derme e hipoderme, desempenha um papel fundamental na preservação da vida. Fatores intrínsecos e extrínsecos podem acelerar o envelhecimento da pele e comprometer as suas funções homeostáticas. A radiação solar, especialmente a radiação ultravioleta (UV), representa um risco significativo de cancer de pele e acelera o fotoenvelhecimento. Este estudo explora a segurança in vitro e os efeitos protetores dos polifenóis (ácido clorogênico, apigenina, kaempferol e naringenina) contra a peroxidação lipídica induzida por UV usando metodologias inovadoras, incluindo ensaio HET-CAM, HPLC-TBARS-EVSC e fluxometria Laser Doppler. O ensaio HET-CAM confirmou os perfis não irritantes para os polifenóis. HPLC-TBARS-EVSC revelou que apenas a naringenina exibiu uma redução significativa na peroxidação lipídica, enquanto outros mostraram tendências pró-oxidantes. A fluxometria Laser Doppler demonstrou potencial anti-inflamatório, com a naringenina apresentando eficácia superior na redução da resposta inflamatória induzida pelo nicotinato de metila. Esta investigação abrangente destaca os diversos papéis protetores dos polifenóis na saúde da pele, enfatizando as notáveis propriedades anti-radicais e anti-inflamatórias da naringenina.Biblioteca Digitais de Teses e Dissertações da USPBaby, André RolimRuscinc, Nadia2024-05-24info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttps://www.teses.usp.br/teses/disponiveis/9/9139/tde-22072024-100427/reponame:Biblioteca Digital de Teses e Dissertações da USPinstname:Universidade de São Paulo (USP)instacron:USPLiberar o conteúdo para acesso público.info:eu-repo/semantics/openAccesseng2024-07-23T20:11:02Zoai:teses.usp.br:tde-22072024-100427Biblioteca Digital de Teses e Dissertaçõeshttp://www.teses.usp.br/PUBhttp://www.teses.usp.br/cgi-bin/mtd2br.plvirginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.bropendoar:27212024-07-23T20:11:02Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Cutaneous attributes of chlorogenic acid, apigenin, kaempferol and naringenin established by HPLC-TBARS-EVSC protocol and Laser Doppler flowmetry
Atributos cutâneos do ácido clorogênico, apigenina, kaempferol e naringenina estabelecidos pelo protocolo HPLC-TBARS-EVSC e fluxometria Laser Doppler
title Cutaneous attributes of chlorogenic acid, apigenin, kaempferol and naringenin established by HPLC-TBARS-EVSC protocol and Laser Doppler flowmetry
spellingShingle Cutaneous attributes of chlorogenic acid, apigenin, kaempferol and naringenin established by HPLC-TBARS-EVSC protocol and Laser Doppler flowmetry
Ruscinc, Nadia
Estresss oxidativo
Lipid peroxidation
Oxidative stress
Peroxidação lipídica
Polifenois
Polyphenols
TBARS
TBARS
title_short Cutaneous attributes of chlorogenic acid, apigenin, kaempferol and naringenin established by HPLC-TBARS-EVSC protocol and Laser Doppler flowmetry
title_full Cutaneous attributes of chlorogenic acid, apigenin, kaempferol and naringenin established by HPLC-TBARS-EVSC protocol and Laser Doppler flowmetry
title_fullStr Cutaneous attributes of chlorogenic acid, apigenin, kaempferol and naringenin established by HPLC-TBARS-EVSC protocol and Laser Doppler flowmetry
title_full_unstemmed Cutaneous attributes of chlorogenic acid, apigenin, kaempferol and naringenin established by HPLC-TBARS-EVSC protocol and Laser Doppler flowmetry
title_sort Cutaneous attributes of chlorogenic acid, apigenin, kaempferol and naringenin established by HPLC-TBARS-EVSC protocol and Laser Doppler flowmetry
author Ruscinc, Nadia
author_facet Ruscinc, Nadia
author_role author
dc.contributor.none.fl_str_mv Baby, André Rolim
dc.contributor.author.fl_str_mv Ruscinc, Nadia
dc.subject.por.fl_str_mv Estresss oxidativo
Lipid peroxidation
Oxidative stress
Peroxidação lipídica
Polifenois
Polyphenols
TBARS
TBARS
topic Estresss oxidativo
Lipid peroxidation
Oxidative stress
Peroxidação lipídica
Polifenois
Polyphenols
TBARS
TBARS
description The integumentary system, a vital organ for organisms, constitutes a multifaceted barrier against pathogens and environmental factors, crucial for maintaining homeostasis. Skin, composed of epidermis, dermis, and hypodermis, plays a pivotal role in preserving life. Intrinsic and extrinsic factors can accelerate skin aging and compromise its homeostatic functions. Solar radiation, particularly ultraviolet (UV) radiation, poses a significant risk for skin cancer and accelerates photoaging. This study explores the in vitro safety and protective effects of polyphenols (chlorogenic acid, apigenin, kaempferol, and naringenin) against UV-induced lipid peroxidation using innovative methodologies, including HET-CAM assay, HPLC-TBARS-EVSC, and laser Doppler flowmetry. The HET-CAM assay confirmed non-irritating profiles for the polyphenols. HPLC-TBARS-EVSC revealed that only naringenin exhibited a significant reduction in lipid peroxidation, while others showed pro-oxidant tendencies. Laser Doppler flowmetry demonstrated anti-inflammatory potential, with naringenin displaying superior efficacy in reducing inflammatory response induced by methyl nicotinate. This comprehensive investigation underscores the diverse protective roles of polyphenols in skin health, emphasizing naringenin\'s notable anti-radical and antiinflammatory properties.
publishDate 2024
dc.date.none.fl_str_mv 2024-05-24
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.teses.usp.br/teses/disponiveis/9/9139/tde-22072024-100427/
url https://www.teses.usp.br/teses/disponiveis/9/9139/tde-22072024-100427/
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv
dc.rights.driver.fl_str_mv Liberar o conteúdo para acesso público.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Liberar o conteúdo para acesso público.
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv
dc.publisher.none.fl_str_mv Biblioteca Digitais de Teses e Dissertações da USP
publisher.none.fl_str_mv Biblioteca Digitais de Teses e Dissertações da USP
dc.source.none.fl_str_mv
reponame:Biblioteca Digital de Teses e Dissertações da USP
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Biblioteca Digital de Teses e Dissertações da USP
collection Biblioteca Digital de Teses e Dissertações da USP
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)
repository.mail.fl_str_mv virginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.br
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