Isolation of potential lipolytic filamentous fungi from Macauba samples for applications in biotechnological processes / Isolamento de fungos filamentosos potenciais lipolíticos a partir de amostras de Macaúba visando aplicações em processos biotecnológicos

• Autor(a) principal: Rocha, Adeline Cristina Pereira
• Data de Publicação: 2021
• Outros Autores: Costa, Tatiana Paula, Schmiele, Marcio, Santos, Sandro Luiz Barbosa dos, Roa, Juan Pedro Bretas, Nelson, David Lee, Benassi, Vivian Machado
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Revista Veras
• Texto Completo: https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/29927
• topic: Enzymes; Filamentous fungus; Macaúba.

Lipases have great biotechnological value, and they are used in several industrial sectors, including the food, pharmaceutical and energy sectors, such as in the production of methyl or ethyl esters of fatty acids (biodiesel). The aim of this study was to isolate promising lipolytic filamentous fungi from samples of bark and macauba oil, as well as to analyze the characteristics of the isolates, the effect of temperature on the growth of isolated fungi and the potential for lipase production by fungi. Samples of macauba shells and samples of macauba oil were collected, and nine filamentous fungi with similar morphological macroscopic characteristics were isolated. The fungi grew best at 30 °C in solid culture medium containing olive oil. The qualitative production of lipases was evaluated in a solid culture medium for lipases at 30 °C for 48 hours. Three strains identified as ARO1, ARO2 and ARC3, with enzymatic radii of 0.43 cm, 0.30 cm and 0.27 cm, respectively, were selected. The quantitative production of lipases in submerged Carvalho Peixoto (CP) medium containing glycerol as the carbon source was performed for these three isolates at 30 oC over a 120-hour period. The highest lipolytic activity was observed for the ARC3 fungus (3.81 U.mL-1), followed by ARO1 (1.43 U.mL-1) and ARO2 (0.72 U.mL-1). The filamentous fungus ARC3, isolated from samples of macauba shell, exhibited the greatest potential for lipase production.