Standardization of the cultivation of the isolated filamentous fungus A4 for lipase production / Padronização do cultivo do fungo filamentoso isolado A4 para a produção de lipases
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista Veras |
Texto Completo: | https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/17953 |
Resumo: | Lipases are produced by microorganisms to form an important biotechnological group of enzymes with different properties and specificities. Among the lipase-producing microorganisms are the filamentous fungi, which are abundant and widespread in nature. Because of their catalytic versatility, lipases are highly commercialized, being applied mainly in the production of detergents, food, animal feed, paper and cellulose, pharmaceutical and biodiesel industries. This work sought to select a filamentous fungus that produces lipases and optimize the conditions for cultivation so as to increase lipolytic activity. Initially, 21 filamentous fungi were grown in a solid medium for lipases for four days in a bacteriological incubator at 30 ºC. The fungus with the largest halo representing lipase activity was incubated in five different submerged media at 30 ºC for eight days, and the lipase activity of crude extracellular enzyme extracts was evaluated every 24 hours. After defining the culture medium, its composition was varied, analyzing different sources of nitrogen, salt solutions, the influence of the initial pH of the medium and the carbon source. The isolated fungus, identified as A4, exhibited promising lipase synthesis in Khanna submerged culture medium containing urea, peptone and KNO3 as the nitrogen source, Vogel salts solution, initial pH of 4.5 and cotton oil as a carbon source. This work demonstrated the importance of standardizing chemical conditions for the growth and production of lipases from filamentous fungi, thereby contributing to a greater understanding of the lipases produced by the selection of the fungus with a potential for enzyme production. |
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Standardization of the cultivation of the isolated filamentous fungus A4 for lipase production / Padronização do cultivo do fungo filamentoso isolado A4 para a produção de lipasesEnzymesFilamentous fungusBiotechnologyLipases are produced by microorganisms to form an important biotechnological group of enzymes with different properties and specificities. Among the lipase-producing microorganisms are the filamentous fungi, which are abundant and widespread in nature. Because of their catalytic versatility, lipases are highly commercialized, being applied mainly in the production of detergents, food, animal feed, paper and cellulose, pharmaceutical and biodiesel industries. This work sought to select a filamentous fungus that produces lipases and optimize the conditions for cultivation so as to increase lipolytic activity. Initially, 21 filamentous fungi were grown in a solid medium for lipases for four days in a bacteriological incubator at 30 ºC. The fungus with the largest halo representing lipase activity was incubated in five different submerged media at 30 ºC for eight days, and the lipase activity of crude extracellular enzyme extracts was evaluated every 24 hours. After defining the culture medium, its composition was varied, analyzing different sources of nitrogen, salt solutions, the influence of the initial pH of the medium and the carbon source. The isolated fungus, identified as A4, exhibited promising lipase synthesis in Khanna submerged culture medium containing urea, peptone and KNO3 as the nitrogen source, Vogel salts solution, initial pH of 4.5 and cotton oil as a carbon source. This work demonstrated the importance of standardizing chemical conditions for the growth and production of lipases from filamentous fungi, thereby contributing to a greater understanding of the lipases produced by the selection of the fungus with a potential for enzyme production.Brazilian Journals Publicações de Periódicos e Editora Ltda.2020-10-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/1795310.34117/bjdv6n10-168Brazilian Journal of Development; Vol. 6 No. 10 (2020); 76404-76423Brazilian Journal of Development; Vol. 6 Núm. 10 (2020); 76404-76423Brazilian Journal of Development; v. 6 n. 10 (2020); 76404-764232525-8761reponame:Revista Verasinstname:Instituto Superior de Educação Vera Cruz (VeraCruz)instacron:VERACRUZporhttps://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/17953/14536Copyright (c) 2020 Brazilian Journal of Developmentinfo:eu-repo/semantics/openAccessCosta, Tatiana P.Spencer, Paula V. D.Souza, Mirian J.Rocha, Adeline C. P.Nelson, David L.Pinto, Nísia A. V. D.Benassi, Vivian M.2021-02-04T12:50:18Zoai:ojs2.ojs.brazilianjournals.com.br:article/17953Revistahttp://site.veracruz.edu.br:8087/instituto/revistaveras/index.php/revistaveras/PRIhttp://site.veracruz.edu.br:8087/instituto/revistaveras/index.php/revistaveras/oai||revistaveras@veracruz.edu.br2236-57292236-5729opendoar:2024-10-15T16:10:19.502770Revista Veras - Instituto Superior de Educação Vera Cruz (VeraCruz)false |
dc.title.none.fl_str_mv |
Standardization of the cultivation of the isolated filamentous fungus A4 for lipase production / Padronização do cultivo do fungo filamentoso isolado A4 para a produção de lipases |
title |
Standardization of the cultivation of the isolated filamentous fungus A4 for lipase production / Padronização do cultivo do fungo filamentoso isolado A4 para a produção de lipases |
spellingShingle |
Standardization of the cultivation of the isolated filamentous fungus A4 for lipase production / Padronização do cultivo do fungo filamentoso isolado A4 para a produção de lipases Costa, Tatiana P. Enzymes Filamentous fungus Biotechnology |
title_short |
Standardization of the cultivation of the isolated filamentous fungus A4 for lipase production / Padronização do cultivo do fungo filamentoso isolado A4 para a produção de lipases |
title_full |
Standardization of the cultivation of the isolated filamentous fungus A4 for lipase production / Padronização do cultivo do fungo filamentoso isolado A4 para a produção de lipases |
title_fullStr |
Standardization of the cultivation of the isolated filamentous fungus A4 for lipase production / Padronização do cultivo do fungo filamentoso isolado A4 para a produção de lipases |
title_full_unstemmed |
Standardization of the cultivation of the isolated filamentous fungus A4 for lipase production / Padronização do cultivo do fungo filamentoso isolado A4 para a produção de lipases |
title_sort |
Standardization of the cultivation of the isolated filamentous fungus A4 for lipase production / Padronização do cultivo do fungo filamentoso isolado A4 para a produção de lipases |
author |
Costa, Tatiana P. |
author_facet |
Costa, Tatiana P. Spencer, Paula V. D. Souza, Mirian J. Rocha, Adeline C. P. Nelson, David L. Pinto, Nísia A. V. D. Benassi, Vivian M. |
author_role |
author |
author2 |
Spencer, Paula V. D. Souza, Mirian J. Rocha, Adeline C. P. Nelson, David L. Pinto, Nísia A. V. D. Benassi, Vivian M. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Costa, Tatiana P. Spencer, Paula V. D. Souza, Mirian J. Rocha, Adeline C. P. Nelson, David L. Pinto, Nísia A. V. D. Benassi, Vivian M. |
dc.subject.por.fl_str_mv |
Enzymes Filamentous fungus Biotechnology |
topic |
Enzymes Filamentous fungus Biotechnology |
description |
Lipases are produced by microorganisms to form an important biotechnological group of enzymes with different properties and specificities. Among the lipase-producing microorganisms are the filamentous fungi, which are abundant and widespread in nature. Because of their catalytic versatility, lipases are highly commercialized, being applied mainly in the production of detergents, food, animal feed, paper and cellulose, pharmaceutical and biodiesel industries. This work sought to select a filamentous fungus that produces lipases and optimize the conditions for cultivation so as to increase lipolytic activity. Initially, 21 filamentous fungi were grown in a solid medium for lipases for four days in a bacteriological incubator at 30 ºC. The fungus with the largest halo representing lipase activity was incubated in five different submerged media at 30 ºC for eight days, and the lipase activity of crude extracellular enzyme extracts was evaluated every 24 hours. After defining the culture medium, its composition was varied, analyzing different sources of nitrogen, salt solutions, the influence of the initial pH of the medium and the carbon source. The isolated fungus, identified as A4, exhibited promising lipase synthesis in Khanna submerged culture medium containing urea, peptone and KNO3 as the nitrogen source, Vogel salts solution, initial pH of 4.5 and cotton oil as a carbon source. This work demonstrated the importance of standardizing chemical conditions for the growth and production of lipases from filamentous fungi, thereby contributing to a greater understanding of the lipases produced by the selection of the fungus with a potential for enzyme production. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-10-08 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/17953 10.34117/bjdv6n10-168 |
url |
https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/17953 |
identifier_str_mv |
10.34117/bjdv6n10-168 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/17953/14536 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Brazilian Journal of Development info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Brazilian Journal of Development |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Brazilian Journals Publicações de Periódicos e Editora Ltda. |
publisher.none.fl_str_mv |
Brazilian Journals Publicações de Periódicos e Editora Ltda. |
dc.source.none.fl_str_mv |
Brazilian Journal of Development; Vol. 6 No. 10 (2020); 76404-76423 Brazilian Journal of Development; Vol. 6 Núm. 10 (2020); 76404-76423 Brazilian Journal of Development; v. 6 n. 10 (2020); 76404-76423 2525-8761 reponame:Revista Veras instname:Instituto Superior de Educação Vera Cruz (VeraCruz) instacron:VERACRUZ |
instname_str |
Instituto Superior de Educação Vera Cruz (VeraCruz) |
instacron_str |
VERACRUZ |
institution |
VERACRUZ |
reponame_str |
Revista Veras |
collection |
Revista Veras |
repository.name.fl_str_mv |
Revista Veras - Instituto Superior de Educação Vera Cruz (VeraCruz) |
repository.mail.fl_str_mv |
||revistaveras@veracruz.edu.br |
_version_ |
1813645483660279808 |