Standardization of the cultivation of the isolated filamentous fungus A4 for lipase production / Padronização do cultivo do fungo filamentoso isolado A4 para a produção de lipases

Detalhes bibliográficos
Autor(a) principal: Costa, Tatiana P.
Data de Publicação: 2020
Outros Autores: Spencer, Paula V. D., Souza, Mirian J., Rocha, Adeline C. P., Nelson, David L., Pinto, Nísia A. V. D., Benassi, Vivian M.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista Veras
Texto Completo: https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/17953
Resumo: Lipases are produced by microorganisms to form an important biotechnological group of enzymes with different properties and specificities. Among the lipase-producing microorganisms are the filamentous fungi, which are abundant and widespread in nature. Because of their catalytic versatility, lipases are highly commercialized, being applied mainly in the production of detergents, food, animal feed, paper and cellulose, pharmaceutical and biodiesel industries. This work sought to select a filamentous fungus that produces lipases and optimize the conditions for cultivation so as to increase lipolytic activity. Initially, 21 filamentous fungi were grown in a solid medium for lipases for four days in a bacteriological incubator at 30 ºC. The fungus with the largest halo representing lipase activity was incubated in five different submerged media at 30 ºC for eight days, and the lipase activity of crude extracellular enzyme extracts was evaluated every 24 hours. After defining the culture medium, its composition was varied, analyzing different sources of nitrogen, salt solutions, the influence of the initial pH of the medium and the carbon source. The isolated fungus, identified as A4, exhibited promising lipase synthesis in Khanna submerged culture medium containing urea, peptone and KNO3 as the nitrogen source, Vogel salts solution, initial pH of 4.5 and cotton oil as a carbon source. This work demonstrated the importance of standardizing chemical conditions for the growth and production of lipases from filamentous fungi, thereby contributing to a greater understanding of the lipases produced by the selection of the fungus with a potential for enzyme production.
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spelling Standardization of the cultivation of the isolated filamentous fungus A4 for lipase production / Padronização do cultivo do fungo filamentoso isolado A4 para a produção de lipasesEnzymesFilamentous fungusBiotechnologyLipases are produced by microorganisms to form an important biotechnological group of enzymes with different properties and specificities. Among the lipase-producing microorganisms are the filamentous fungi, which are abundant and widespread in nature. Because of their catalytic versatility, lipases are highly commercialized, being applied mainly in the production of detergents, food, animal feed, paper and cellulose, pharmaceutical and biodiesel industries. This work sought to select a filamentous fungus that produces lipases and optimize the conditions for cultivation so as to increase lipolytic activity. Initially, 21 filamentous fungi were grown in a solid medium for lipases for four days in a bacteriological incubator at 30 ºC. The fungus with the largest halo representing lipase activity was incubated in five different submerged media at 30 ºC for eight days, and the lipase activity of crude extracellular enzyme extracts was evaluated every 24 hours. After defining the culture medium, its composition was varied, analyzing different sources of nitrogen, salt solutions, the influence of the initial pH of the medium and the carbon source. The isolated fungus, identified as A4, exhibited promising lipase synthesis in Khanna submerged culture medium containing urea, peptone and KNO3 as the nitrogen source, Vogel salts solution, initial pH of 4.5 and cotton oil as a carbon source. This work demonstrated the importance of standardizing chemical conditions for the growth and production of lipases from filamentous fungi, thereby contributing to a greater understanding of the lipases produced by the selection of the fungus with a potential for enzyme production.Brazilian Journals Publicações de Periódicos e Editora Ltda.2020-10-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/1795310.34117/bjdv6n10-168Brazilian Journal of Development; Vol. 6 No. 10 (2020); 76404-76423Brazilian Journal of Development; Vol. 6 Núm. 10 (2020); 76404-76423Brazilian Journal of Development; v. 6 n. 10 (2020); 76404-764232525-8761reponame:Revista Verasinstname:Instituto Superior de Educação Vera Cruz (VeraCruz)instacron:VERACRUZporhttps://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/17953/14536Copyright (c) 2020 Brazilian Journal of Developmentinfo:eu-repo/semantics/openAccessCosta, Tatiana P.Spencer, Paula V. D.Souza, Mirian J.Rocha, Adeline C. P.Nelson, David L.Pinto, Nísia A. V. D.Benassi, Vivian M.2021-02-04T12:50:18Zoai:ojs2.ojs.brazilianjournals.com.br:article/17953Revistahttp://site.veracruz.edu.br:8087/instituto/revistaveras/index.php/revistaveras/PRIhttp://site.veracruz.edu.br:8087/instituto/revistaveras/index.php/revistaveras/oai||revistaveras@veracruz.edu.br2236-57292236-5729opendoar:2024-10-15T16:10:19.502770Revista Veras - Instituto Superior de Educação Vera Cruz (VeraCruz)false
dc.title.none.fl_str_mv Standardization of the cultivation of the isolated filamentous fungus A4 for lipase production / Padronização do cultivo do fungo filamentoso isolado A4 para a produção de lipases
title Standardization of the cultivation of the isolated filamentous fungus A4 for lipase production / Padronização do cultivo do fungo filamentoso isolado A4 para a produção de lipases
spellingShingle Standardization of the cultivation of the isolated filamentous fungus A4 for lipase production / Padronização do cultivo do fungo filamentoso isolado A4 para a produção de lipases
Costa, Tatiana P.
Enzymes
Filamentous fungus
Biotechnology
title_short Standardization of the cultivation of the isolated filamentous fungus A4 for lipase production / Padronização do cultivo do fungo filamentoso isolado A4 para a produção de lipases
title_full Standardization of the cultivation of the isolated filamentous fungus A4 for lipase production / Padronização do cultivo do fungo filamentoso isolado A4 para a produção de lipases
title_fullStr Standardization of the cultivation of the isolated filamentous fungus A4 for lipase production / Padronização do cultivo do fungo filamentoso isolado A4 para a produção de lipases
title_full_unstemmed Standardization of the cultivation of the isolated filamentous fungus A4 for lipase production / Padronização do cultivo do fungo filamentoso isolado A4 para a produção de lipases
title_sort Standardization of the cultivation of the isolated filamentous fungus A4 for lipase production / Padronização do cultivo do fungo filamentoso isolado A4 para a produção de lipases
author Costa, Tatiana P.
author_facet Costa, Tatiana P.
Spencer, Paula V. D.
Souza, Mirian J.
Rocha, Adeline C. P.
Nelson, David L.
Pinto, Nísia A. V. D.
Benassi, Vivian M.
author_role author
author2 Spencer, Paula V. D.
Souza, Mirian J.
Rocha, Adeline C. P.
Nelson, David L.
Pinto, Nísia A. V. D.
Benassi, Vivian M.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Costa, Tatiana P.
Spencer, Paula V. D.
Souza, Mirian J.
Rocha, Adeline C. P.
Nelson, David L.
Pinto, Nísia A. V. D.
Benassi, Vivian M.
dc.subject.por.fl_str_mv Enzymes
Filamentous fungus
Biotechnology
topic Enzymes
Filamentous fungus
Biotechnology
description Lipases are produced by microorganisms to form an important biotechnological group of enzymes with different properties and specificities. Among the lipase-producing microorganisms are the filamentous fungi, which are abundant and widespread in nature. Because of their catalytic versatility, lipases are highly commercialized, being applied mainly in the production of detergents, food, animal feed, paper and cellulose, pharmaceutical and biodiesel industries. This work sought to select a filamentous fungus that produces lipases and optimize the conditions for cultivation so as to increase lipolytic activity. Initially, 21 filamentous fungi were grown in a solid medium for lipases for four days in a bacteriological incubator at 30 ºC. The fungus with the largest halo representing lipase activity was incubated in five different submerged media at 30 ºC for eight days, and the lipase activity of crude extracellular enzyme extracts was evaluated every 24 hours. After defining the culture medium, its composition was varied, analyzing different sources of nitrogen, salt solutions, the influence of the initial pH of the medium and the carbon source. The isolated fungus, identified as A4, exhibited promising lipase synthesis in Khanna submerged culture medium containing urea, peptone and KNO3 as the nitrogen source, Vogel salts solution, initial pH of 4.5 and cotton oil as a carbon source. This work demonstrated the importance of standardizing chemical conditions for the growth and production of lipases from filamentous fungi, thereby contributing to a greater understanding of the lipases produced by the selection of the fungus with a potential for enzyme production.
publishDate 2020
dc.date.none.fl_str_mv 2020-10-08
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/17953
10.34117/bjdv6n10-168
url https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/17953
identifier_str_mv 10.34117/bjdv6n10-168
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/17953/14536
dc.rights.driver.fl_str_mv Copyright (c) 2020 Brazilian Journal of Development
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Brazilian Journal of Development
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Brazilian Journals Publicações de Periódicos e Editora Ltda.
publisher.none.fl_str_mv Brazilian Journals Publicações de Periódicos e Editora Ltda.
dc.source.none.fl_str_mv Brazilian Journal of Development; Vol. 6 No. 10 (2020); 76404-76423
Brazilian Journal of Development; Vol. 6 Núm. 10 (2020); 76404-76423
Brazilian Journal of Development; v. 6 n. 10 (2020); 76404-76423
2525-8761
reponame:Revista Veras
instname:Instituto Superior de Educação Vera Cruz (VeraCruz)
instacron:VERACRUZ
instname_str Instituto Superior de Educação Vera Cruz (VeraCruz)
instacron_str VERACRUZ
institution VERACRUZ
reponame_str Revista Veras
collection Revista Veras
repository.name.fl_str_mv Revista Veras - Instituto Superior de Educação Vera Cruz (VeraCruz)
repository.mail.fl_str_mv ||revistaveras@veracruz.edu.br
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