Extracting DNA of nematodes communities from Argentine Pampas agricultural soils

Detalhes bibliográficos
Autor(a) principal: Mondino,Eduardo A.
Data de Publicação: 2015
Outros Autores: Covacevich,Fernanda, Studdert,Guillermo A., Pimentel,João P., Berbara,Ricardo L.L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Anais da Academia Brasileira de Ciências (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200691
Resumo: We examined four strategies (Tris/EDTA, sodium dodecyl sulfate, Chelex 100 resin and cetyltrimethylammonium bromide -CTAB-) for extracting nucleic acid (DNA) from communities of nematodes. Nematodes were isolated from an agricultural area under different management of long-term crop rotation experiment from Argentina during three seasons. After DNA extraction, Polymerase Chain Reaction-amplifications were performed and considered as indicators of successful DNA extraction. The CTAB combined with proteinase K and phenol-chloroform-isoamyl alcohol was the unique successful method because positive amplifications were obtained by using both eukaryotic and nematode specific primers. This work could contribute to biodiversity studies of nematodes on agroecosystems.
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spelling Extracting DNA of nematodes communities from Argentine Pampas agricultural soilsAgroecosystemsnematodesnucleic acid extractionCTABWe examined four strategies (Tris/EDTA, sodium dodecyl sulfate, Chelex 100 resin and cetyltrimethylammonium bromide -CTAB-) for extracting nucleic acid (DNA) from communities of nematodes. Nematodes were isolated from an agricultural area under different management of long-term crop rotation experiment from Argentina during three seasons. After DNA extraction, Polymerase Chain Reaction-amplifications were performed and considered as indicators of successful DNA extraction. The CTAB combined with proteinase K and phenol-chloroform-isoamyl alcohol was the unique successful method because positive amplifications were obtained by using both eukaryotic and nematode specific primers. This work could contribute to biodiversity studies of nematodes on agroecosystems.Academia Brasileira de Ciências2015-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200691Anais da Academia Brasileira de Ciências v.87 n.2 2015reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765201520130110info:eu-repo/semantics/openAccessMondino,Eduardo A.Covacevich,FernandaStuddert,Guillermo A.Pimentel,João P.Berbara,Ricardo L.L.eng2016-03-04T00:00:00Zoai:scielo:S0001-37652015000200691Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2016-03-04T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false
dc.title.none.fl_str_mv Extracting DNA of nematodes communities from Argentine Pampas agricultural soils
title Extracting DNA of nematodes communities from Argentine Pampas agricultural soils
spellingShingle Extracting DNA of nematodes communities from Argentine Pampas agricultural soils
Mondino,Eduardo A.
Agroecosystems
nematodes
nucleic acid extraction
CTAB
title_short Extracting DNA of nematodes communities from Argentine Pampas agricultural soils
title_full Extracting DNA of nematodes communities from Argentine Pampas agricultural soils
title_fullStr Extracting DNA of nematodes communities from Argentine Pampas agricultural soils
title_full_unstemmed Extracting DNA of nematodes communities from Argentine Pampas agricultural soils
title_sort Extracting DNA of nematodes communities from Argentine Pampas agricultural soils
author Mondino,Eduardo A.
author_facet Mondino,Eduardo A.
Covacevich,Fernanda
Studdert,Guillermo A.
Pimentel,João P.
Berbara,Ricardo L.L.
author_role author
author2 Covacevich,Fernanda
Studdert,Guillermo A.
Pimentel,João P.
Berbara,Ricardo L.L.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Mondino,Eduardo A.
Covacevich,Fernanda
Studdert,Guillermo A.
Pimentel,João P.
Berbara,Ricardo L.L.
dc.subject.por.fl_str_mv Agroecosystems
nematodes
nucleic acid extraction
CTAB
topic Agroecosystems
nematodes
nucleic acid extraction
CTAB
description We examined four strategies (Tris/EDTA, sodium dodecyl sulfate, Chelex 100 resin and cetyltrimethylammonium bromide -CTAB-) for extracting nucleic acid (DNA) from communities of nematodes. Nematodes were isolated from an agricultural area under different management of long-term crop rotation experiment from Argentina during three seasons. After DNA extraction, Polymerase Chain Reaction-amplifications were performed and considered as indicators of successful DNA extraction. The CTAB combined with proteinase K and phenol-chloroform-isoamyl alcohol was the unique successful method because positive amplifications were obtained by using both eukaryotic and nematode specific primers. This work could contribute to biodiversity studies of nematodes on agroecosystems.
publishDate 2015
dc.date.none.fl_str_mv 2015-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200691
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200691
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0001-3765201520130110
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências v.87 n.2 2015
reponame:Anais da Academia Brasileira de Ciências (Online)
instname:Academia Brasileira de Ciências (ABC)
instacron:ABC
instname_str Academia Brasileira de Ciências (ABC)
instacron_str ABC
institution ABC
reponame_str Anais da Academia Brasileira de Ciências (Online)
collection Anais da Academia Brasileira de Ciências (Online)
repository.name.fl_str_mv Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)
repository.mail.fl_str_mv ||aabc@abc.org.br
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