Wound healing and anti-oxidant activities induced by an alginate hydrogel-based ointment

• Autor(a) principal: Cabrera, Tatiana Noel Basika
• Data de Publicação: 2023
• Outros Autores: Greif, Gonzalo, Giusti, Marcos, Bollati-Fogolín, Mariela
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Journal of Health Review
• Texto Completo: https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/63788
• topic: hydrogel; wound healing; sodium alginate; tissue repair

Background: Alginate, a natural polysaccharide derived from seaweed, has shown to be better than other modern dressings in reducing wound area. The Brazilian population and medical care staff have used hydrogel-based ointment containing alginate for years in treatment of acute and chronic wounds. It is popularly known for their benefits, but so far, no molecular and cellular scientific proof of their wound healing capability has been tested. Objective: Since there is little information about the molecular and cellular responses of the hydrogel-based ointment containing alginate to improve wound healing mechanisms, in this study we evaluate the wound healing capability of a specific hydrogel employing in vitro models of human skin keratinocytes and fibroblastos. Methods: HaCaT-SF and HDFn cells were used as models of human skin keratinocytes and fibroblasts, respectively. The ointment effects in the wound healing were evaluated specifically on cell proliferation and migration, analyzing gene expression profiles and soluble factors secretion, as well as antioxidant activity. Results: From the analysis several genes related to the wound healing stages showed differential expression and secretion patterns in ointment-treated samples compared with control ones. Among these, cytokines and chemokines (involved in inflammation); growth factors and angiogenesis related proteins (implied in cell proliferation); matrix metalloproteinases and serine protease inhibitors (engaged in cell migration and tissue remodeling) were identified. Furthermore, this hydrogel exhibited anti-oxidant properties against oxidative damage in keratinocytes. Conclusions: Overall, our results indicate that this hydrogel-based ointment may function as a modulator of the wound healing process, promoting homeostasis and correct tissue repair.