Large-scale, high-efficiency production of coffee somatic embryos

Detalhes bibliográficos
Autor(a) principal: Maciel,Anna Lygia de Rezende
Data de Publicação: 2016
Outros Autores: Rodrigues,Filipe Almendagna, Pasqual,Moacir, Carvalho,Carlos Henrique Siqueira de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Crop Breeding and Applied Biotechnology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332016000200102
Resumo: Abstract The study aims to compare the efficiency of previously used liquid media for coffee, evaluating NAA concentrations in liquid medium and proline in semi-solid medium in the regeneration of somatic embryos and asses concentrations of BA and IAA in the maturation of embryos in temporary immersion bioreactors. For the regeneration of globular embryos from embryogenic aggregates and calli, we tested five concentrations (0.00, 0.25, 0.50, 1.00 and 2.00 mg L-1) of NAA in liquid medium and five concentrations (0.0, 0.5, 1.0, 2.0 and 4.0 g L-1) of proline in semisolid medium. The multiplication of embryogenic aggregates was highest in culture medium MM, reaching a density 7.5 times greater than that of the initial density. NAA promoted a linear increase in embryo regeneration. The medium containing 2.0 mg L-1 BA and 0.0 mg L-1 IAA yielded the highest percentage of large cotyledonary embryos.
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spelling Large-scale, high-efficiency production of coffee somatic embryosVegetative propagationbiotechnologytissue cultureCoffea arabica LAbstract The study aims to compare the efficiency of previously used liquid media for coffee, evaluating NAA concentrations in liquid medium and proline in semi-solid medium in the regeneration of somatic embryos and asses concentrations of BA and IAA in the maturation of embryos in temporary immersion bioreactors. For the regeneration of globular embryos from embryogenic aggregates and calli, we tested five concentrations (0.00, 0.25, 0.50, 1.00 and 2.00 mg L-1) of NAA in liquid medium and five concentrations (0.0, 0.5, 1.0, 2.0 and 4.0 g L-1) of proline in semisolid medium. The multiplication of embryogenic aggregates was highest in culture medium MM, reaching a density 7.5 times greater than that of the initial density. NAA promoted a linear increase in embryo regeneration. The medium containing 2.0 mg L-1 BA and 0.0 mg L-1 IAA yielded the highest percentage of large cotyledonary embryos.Crop Breeding and Applied Biotechnology2016-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332016000200102Crop Breeding and Applied Biotechnology v.16 n.2 2016reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332016v16n2a16info:eu-repo/semantics/openAccessMaciel,Anna Lygia de RezendeRodrigues,Filipe AlmendagnaPasqual,MoacirCarvalho,Carlos Henrique Siqueira deeng2016-08-01T00:00:00Zoai:scielo:S1984-70332016000200102Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2016-08-01T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse
dc.title.none.fl_str_mv Large-scale, high-efficiency production of coffee somatic embryos
title Large-scale, high-efficiency production of coffee somatic embryos
spellingShingle Large-scale, high-efficiency production of coffee somatic embryos
Maciel,Anna Lygia de Rezende
Vegetative propagation
biotechnology
tissue culture
Coffea arabica L
title_short Large-scale, high-efficiency production of coffee somatic embryos
title_full Large-scale, high-efficiency production of coffee somatic embryos
title_fullStr Large-scale, high-efficiency production of coffee somatic embryos
title_full_unstemmed Large-scale, high-efficiency production of coffee somatic embryos
title_sort Large-scale, high-efficiency production of coffee somatic embryos
author Maciel,Anna Lygia de Rezende
author_facet Maciel,Anna Lygia de Rezende
Rodrigues,Filipe Almendagna
Pasqual,Moacir
Carvalho,Carlos Henrique Siqueira de
author_role author
author2 Rodrigues,Filipe Almendagna
Pasqual,Moacir
Carvalho,Carlos Henrique Siqueira de
author2_role author
author
author
dc.contributor.author.fl_str_mv Maciel,Anna Lygia de Rezende
Rodrigues,Filipe Almendagna
Pasqual,Moacir
Carvalho,Carlos Henrique Siqueira de
dc.subject.por.fl_str_mv Vegetative propagation
biotechnology
tissue culture
Coffea arabica L
topic Vegetative propagation
biotechnology
tissue culture
Coffea arabica L
description Abstract The study aims to compare the efficiency of previously used liquid media for coffee, evaluating NAA concentrations in liquid medium and proline in semi-solid medium in the regeneration of somatic embryos and asses concentrations of BA and IAA in the maturation of embryos in temporary immersion bioreactors. For the regeneration of globular embryos from embryogenic aggregates and calli, we tested five concentrations (0.00, 0.25, 0.50, 1.00 and 2.00 mg L-1) of NAA in liquid medium and five concentrations (0.0, 0.5, 1.0, 2.0 and 4.0 g L-1) of proline in semisolid medium. The multiplication of embryogenic aggregates was highest in culture medium MM, reaching a density 7.5 times greater than that of the initial density. NAA promoted a linear increase in embryo regeneration. The medium containing 2.0 mg L-1 BA and 0.0 mg L-1 IAA yielded the highest percentage of large cotyledonary embryos.
publishDate 2016
dc.date.none.fl_str_mv 2016-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332016000200102
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332016000200102
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1984-70332016v16n2a16
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology v.16 n.2 2016
reponame:Crop Breeding and Applied Biotechnology
instname:Sociedade Brasileira de Melhoramento de Plantas
instacron:CBAB
instname_str Sociedade Brasileira de Melhoramento de Plantas
instacron_str CBAB
institution CBAB
reponame_str Crop Breeding and Applied Biotechnology
collection Crop Breeding and Applied Biotechnology
repository.name.fl_str_mv Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas
repository.mail.fl_str_mv cbabjournal@gmail.com||cbab@ufv.br
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