Large-scale, high-efficiency production of coffee somatic embryos

Detalhes bibliográficos
Autor(a) principal: Maciel, Anna Lygia de Rezende
Data de Publicação: 2016
Outros Autores: Rodrigues, Filipe Almendagna, Pasqual, Moacir, Carvalho, Carlos Henrique Siqueira de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/32819
Resumo: The study aims to compare the efficiency of previously used liquid media for coffee, evaluating NAA concentrations in liquid medium and proline in semi-solid medium in the regeneration of somatic embryos and asses concentrations of BA and IAA in the maturation of embryos in temporary immersion bioreactors. For the regeneration of globular embryos from embryogenic aggregates and calli, we tested five concentrations (0.00, 0.25, 0.50, 1.00 and 2.00 mg L-1) of NAA in liquid medium and five concentrations (0.0, 0.5, 1.0, 2.0 and 4.0 g L-1) of proline in semisolid medium. The multiplication of embryogenic aggregates was highest in culture medium MM, reaching a density 7.5 times greater than that of the initial density. NAA promoted a linear increase in embryo regeneration. The medium containing 2.0 mg L-1 BA and 0.0 mg L-1 IAA yielded the highest percentage of large cotyledonary embryos.
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spelling Large-scale, high-efficiency production of coffee somatic embryosVegetative propagationBiotechnologyTissue cultureCotyledonary embryosPropagação vegetativaBiotecnologiaCultura de tecidosEmbriões cotiledonaresThe study aims to compare the efficiency of previously used liquid media for coffee, evaluating NAA concentrations in liquid medium and proline in semi-solid medium in the regeneration of somatic embryos and asses concentrations of BA and IAA in the maturation of embryos in temporary immersion bioreactors. For the regeneration of globular embryos from embryogenic aggregates and calli, we tested five concentrations (0.00, 0.25, 0.50, 1.00 and 2.00 mg L-1) of NAA in liquid medium and five concentrations (0.0, 0.5, 1.0, 2.0 and 4.0 g L-1) of proline in semisolid medium. The multiplication of embryogenic aggregates was highest in culture medium MM, reaching a density 7.5 times greater than that of the initial density. NAA promoted a linear increase in embryo regeneration. The medium containing 2.0 mg L-1 BA and 0.0 mg L-1 IAA yielded the highest percentage of large cotyledonary embryos.Crop Breeding and Applied Biotechnology2019-02-15T14:32:11Z2019-02-15T14:32:11Z2016-04info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfMACIEL, A. L. de R. et al. Large-scale, high-efficiency production of coffee somatic embryos. Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 16, n. 2, p. 102-107, Apr./June 2017.http://repositorio.ufla.br/jspui/handle/1/32819Crop Breeding and Applied Biotechnologyreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessMaciel, Anna Lygia de RezendeRodrigues, Filipe AlmendagnaPasqual, MoacirCarvalho, Carlos Henrique Siqueira deeng2023-05-26T18:43:58Zoai:localhost:1/32819Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-26T18:43:58Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Large-scale, high-efficiency production of coffee somatic embryos
title Large-scale, high-efficiency production of coffee somatic embryos
spellingShingle Large-scale, high-efficiency production of coffee somatic embryos
Maciel, Anna Lygia de Rezende
Vegetative propagation
Biotechnology
Tissue culture
Cotyledonary embryos
Propagação vegetativa
Biotecnologia
Cultura de tecidos
Embriões cotiledonares
title_short Large-scale, high-efficiency production of coffee somatic embryos
title_full Large-scale, high-efficiency production of coffee somatic embryos
title_fullStr Large-scale, high-efficiency production of coffee somatic embryos
title_full_unstemmed Large-scale, high-efficiency production of coffee somatic embryos
title_sort Large-scale, high-efficiency production of coffee somatic embryos
author Maciel, Anna Lygia de Rezende
author_facet Maciel, Anna Lygia de Rezende
Rodrigues, Filipe Almendagna
Pasqual, Moacir
Carvalho, Carlos Henrique Siqueira de
author_role author
author2 Rodrigues, Filipe Almendagna
Pasqual, Moacir
Carvalho, Carlos Henrique Siqueira de
author2_role author
author
author
dc.contributor.author.fl_str_mv Maciel, Anna Lygia de Rezende
Rodrigues, Filipe Almendagna
Pasqual, Moacir
Carvalho, Carlos Henrique Siqueira de
dc.subject.por.fl_str_mv Vegetative propagation
Biotechnology
Tissue culture
Cotyledonary embryos
Propagação vegetativa
Biotecnologia
Cultura de tecidos
Embriões cotiledonares
topic Vegetative propagation
Biotechnology
Tissue culture
Cotyledonary embryos
Propagação vegetativa
Biotecnologia
Cultura de tecidos
Embriões cotiledonares
description The study aims to compare the efficiency of previously used liquid media for coffee, evaluating NAA concentrations in liquid medium and proline in semi-solid medium in the regeneration of somatic embryos and asses concentrations of BA and IAA in the maturation of embryos in temporary immersion bioreactors. For the regeneration of globular embryos from embryogenic aggregates and calli, we tested five concentrations (0.00, 0.25, 0.50, 1.00 and 2.00 mg L-1) of NAA in liquid medium and five concentrations (0.0, 0.5, 1.0, 2.0 and 4.0 g L-1) of proline in semisolid medium. The multiplication of embryogenic aggregates was highest in culture medium MM, reaching a density 7.5 times greater than that of the initial density. NAA promoted a linear increase in embryo regeneration. The medium containing 2.0 mg L-1 BA and 0.0 mg L-1 IAA yielded the highest percentage of large cotyledonary embryos.
publishDate 2016
dc.date.none.fl_str_mv 2016-04
2019-02-15T14:32:11Z
2019-02-15T14:32:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv MACIEL, A. L. de R. et al. Large-scale, high-efficiency production of coffee somatic embryos. Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 16, n. 2, p. 102-107, Apr./June 2017.
http://repositorio.ufla.br/jspui/handle/1/32819
identifier_str_mv MACIEL, A. L. de R. et al. Large-scale, high-efficiency production of coffee somatic embryos. Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 16, n. 2, p. 102-107, Apr./June 2017.
url http://repositorio.ufla.br/jspui/handle/1/32819
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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