Large-scale, high-efficiency production of coffee somatic embryos.
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1160431 http://dx.doi.org/10.1590/198470332016v16n2a16 |
Resumo: | The study aims to compare the efficiency of previously used liquid media for coffee, evaluating NAA concentrations in liquid medium and proline in semi-solid medium in the regeneration of somatic embryos and asses concentrations of BA and IAA in the maturation of embryos in temporary immersion bioreactors. For the regeneration of globular embryos from embryogenic aggregates and calli, we tested five concentrations (0.00, 0.25, 0.50, 1.00 and 2.00 mg L-1) of NAA in liquid medium and five concentrations (0.0, 0.5, 1.0, 2.0 and 4.0 g L-1) of proline in semisolid medium. The multiplication of embryogenic aggregates was highest in culture medium MM, reaching a density 7.5 times greater than that of the initial density. NAA promoted a linear increase in embryo regeneration. The medium containing 2.0 mg L-1 BA and 0.0 mg L-1 IAA yielded the highest percentage of large cotyledonary embryos. |
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Large-scale, high-efficiency production of coffee somatic embryos.Vegetative propagationBiotechnologyTissue cultureCoffea arabica var. arabicaThe study aims to compare the efficiency of previously used liquid media for coffee, evaluating NAA concentrations in liquid medium and proline in semi-solid medium in the regeneration of somatic embryos and asses concentrations of BA and IAA in the maturation of embryos in temporary immersion bioreactors. For the regeneration of globular embryos from embryogenic aggregates and calli, we tested five concentrations (0.00, 0.25, 0.50, 1.00 and 2.00 mg L-1) of NAA in liquid medium and five concentrations (0.0, 0.5, 1.0, 2.0 and 4.0 g L-1) of proline in semisolid medium. The multiplication of embryogenic aggregates was highest in culture medium MM, reaching a density 7.5 times greater than that of the initial density. NAA promoted a linear increase in embryo regeneration. The medium containing 2.0 mg L-1 BA and 0.0 mg L-1 IAA yielded the highest percentage of large cotyledonary embryos.ANNA LYGIA DE REZENDE MACIEL, INSTITUTO FEDERAL DE EDUCAÇÃO, CIÊNCIA E TECNOLOGIA DO SUL DE MINAS GERAIS; FILIPE ALMENDAGNA RODRIGUES, UNIVERSIDADE FEDERAL DE LAVRAS; MOACIR PASQUAL, UNIVERSIDADE FEDERAL DE LAVRAS; CARLOS HENRIQUE S DE CARVALHO, CNPCa.MACIEL, A. L. de R.RODRIGUES, F. A.PASQUAL, M.CARVALHO, C. H. S. de2024-01-04T12:32:10Z2024-01-04T12:32:10Z2024-01-042016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCrop Breeding and Applied Biotechnology, v. 16, n. 2, p. 102-107, 2016.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1160431http://dx.doi.org/10.1590/198470332016v16n2a16enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2024-01-04T12:32:10Zoai:www.alice.cnptia.embrapa.br:doc/1160431Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542024-01-04T12:32:10falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542024-01-04T12:32:10Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Large-scale, high-efficiency production of coffee somatic embryos. |
title |
Large-scale, high-efficiency production of coffee somatic embryos. |
spellingShingle |
Large-scale, high-efficiency production of coffee somatic embryos. MACIEL, A. L. de R. Vegetative propagation Biotechnology Tissue culture Coffea arabica var. arabica |
title_short |
Large-scale, high-efficiency production of coffee somatic embryos. |
title_full |
Large-scale, high-efficiency production of coffee somatic embryos. |
title_fullStr |
Large-scale, high-efficiency production of coffee somatic embryos. |
title_full_unstemmed |
Large-scale, high-efficiency production of coffee somatic embryos. |
title_sort |
Large-scale, high-efficiency production of coffee somatic embryos. |
author |
MACIEL, A. L. de R. |
author_facet |
MACIEL, A. L. de R. RODRIGUES, F. A. PASQUAL, M. CARVALHO, C. H. S. de |
author_role |
author |
author2 |
RODRIGUES, F. A. PASQUAL, M. CARVALHO, C. H. S. de |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
ANNA LYGIA DE REZENDE MACIEL, INSTITUTO FEDERAL DE EDUCAÇÃO, CIÊNCIA E TECNOLOGIA DO SUL DE MINAS GERAIS; FILIPE ALMENDAGNA RODRIGUES, UNIVERSIDADE FEDERAL DE LAVRAS; MOACIR PASQUAL, UNIVERSIDADE FEDERAL DE LAVRAS; CARLOS HENRIQUE S DE CARVALHO, CNPCa. |
dc.contributor.author.fl_str_mv |
MACIEL, A. L. de R. RODRIGUES, F. A. PASQUAL, M. CARVALHO, C. H. S. de |
dc.subject.por.fl_str_mv |
Vegetative propagation Biotechnology Tissue culture Coffea arabica var. arabica |
topic |
Vegetative propagation Biotechnology Tissue culture Coffea arabica var. arabica |
description |
The study aims to compare the efficiency of previously used liquid media for coffee, evaluating NAA concentrations in liquid medium and proline in semi-solid medium in the regeneration of somatic embryos and asses concentrations of BA and IAA in the maturation of embryos in temporary immersion bioreactors. For the regeneration of globular embryos from embryogenic aggregates and calli, we tested five concentrations (0.00, 0.25, 0.50, 1.00 and 2.00 mg L-1) of NAA in liquid medium and five concentrations (0.0, 0.5, 1.0, 2.0 and 4.0 g L-1) of proline in semisolid medium. The multiplication of embryogenic aggregates was highest in culture medium MM, reaching a density 7.5 times greater than that of the initial density. NAA promoted a linear increase in embryo regeneration. The medium containing 2.0 mg L-1 BA and 0.0 mg L-1 IAA yielded the highest percentage of large cotyledonary embryos. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2024-01-04T12:32:10Z 2024-01-04T12:32:10Z 2024-01-04 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Crop Breeding and Applied Biotechnology, v. 16, n. 2, p. 102-107, 2016. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1160431 http://dx.doi.org/10.1590/198470332016v16n2a16 |
identifier_str_mv |
Crop Breeding and Applied Biotechnology, v. 16, n. 2, p. 102-107, 2016. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1160431 http://dx.doi.org/10.1590/198470332016v16n2a16 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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