Large-scale, high-efficiency production of coffee somatic embryos.

Detalhes bibliográficos
Autor(a) principal: MACIEL, A. L. de R.
Data de Publicação: 2016
Outros Autores: RODRIGUES, F. A., PASQUAL, M., CARVALHO, C. H. S. de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1160431
http://dx.doi.org/10.1590/198470332016v16n2a16
Resumo: The study aims to compare the efficiency of previously used liquid media for coffee, evaluating NAA concentrations in liquid medium and proline in semi-solid medium in the regeneration of somatic embryos and asses concentrations of BA and IAA in the maturation of embryos in temporary immersion bioreactors. For the regeneration of globular embryos from embryogenic aggregates and calli, we tested five concentrations (0.00, 0.25, 0.50, 1.00 and 2.00 mg L-1) of NAA in liquid medium and five concentrations (0.0, 0.5, 1.0, 2.0 and 4.0 g L-1) of proline in semisolid medium. The multiplication of embryogenic aggregates was highest in culture medium MM, reaching a density 7.5 times greater than that of the initial density. NAA promoted a linear increase in embryo regeneration. The medium containing 2.0 mg L-1 BA and 0.0 mg L-1 IAA yielded the highest percentage of large cotyledonary embryos.
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spelling Large-scale, high-efficiency production of coffee somatic embryos.Vegetative propagationBiotechnologyTissue cultureCoffea arabica var. arabicaThe study aims to compare the efficiency of previously used liquid media for coffee, evaluating NAA concentrations in liquid medium and proline in semi-solid medium in the regeneration of somatic embryos and asses concentrations of BA and IAA in the maturation of embryos in temporary immersion bioreactors. For the regeneration of globular embryos from embryogenic aggregates and calli, we tested five concentrations (0.00, 0.25, 0.50, 1.00 and 2.00 mg L-1) of NAA in liquid medium and five concentrations (0.0, 0.5, 1.0, 2.0 and 4.0 g L-1) of proline in semisolid medium. The multiplication of embryogenic aggregates was highest in culture medium MM, reaching a density 7.5 times greater than that of the initial density. NAA promoted a linear increase in embryo regeneration. The medium containing 2.0 mg L-1 BA and 0.0 mg L-1 IAA yielded the highest percentage of large cotyledonary embryos.ANNA LYGIA DE REZENDE MACIEL, INSTITUTO FEDERAL DE EDUCAÇÃO, CIÊNCIA E TECNOLOGIA DO SUL DE MINAS GERAIS; FILIPE ALMENDAGNA RODRIGUES, UNIVERSIDADE FEDERAL DE LAVRAS; MOACIR PASQUAL, UNIVERSIDADE FEDERAL DE LAVRAS; CARLOS HENRIQUE S DE CARVALHO, CNPCa.MACIEL, A. L. de R.RODRIGUES, F. A.PASQUAL, M.CARVALHO, C. H. S. de2024-01-04T12:32:10Z2024-01-04T12:32:10Z2024-01-042016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCrop Breeding and Applied Biotechnology, v. 16, n. 2, p. 102-107, 2016.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1160431http://dx.doi.org/10.1590/198470332016v16n2a16enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2024-01-04T12:32:10Zoai:www.alice.cnptia.embrapa.br:doc/1160431Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542024-01-04T12:32:10falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542024-01-04T12:32:10Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Large-scale, high-efficiency production of coffee somatic embryos.
title Large-scale, high-efficiency production of coffee somatic embryos.
spellingShingle Large-scale, high-efficiency production of coffee somatic embryos.
MACIEL, A. L. de R.
Vegetative propagation
Biotechnology
Tissue culture
Coffea arabica var. arabica
title_short Large-scale, high-efficiency production of coffee somatic embryos.
title_full Large-scale, high-efficiency production of coffee somatic embryos.
title_fullStr Large-scale, high-efficiency production of coffee somatic embryos.
title_full_unstemmed Large-scale, high-efficiency production of coffee somatic embryos.
title_sort Large-scale, high-efficiency production of coffee somatic embryos.
author MACIEL, A. L. de R.
author_facet MACIEL, A. L. de R.
RODRIGUES, F. A.
PASQUAL, M.
CARVALHO, C. H. S. de
author_role author
author2 RODRIGUES, F. A.
PASQUAL, M.
CARVALHO, C. H. S. de
author2_role author
author
author
dc.contributor.none.fl_str_mv ANNA LYGIA DE REZENDE MACIEL, INSTITUTO FEDERAL DE EDUCAÇÃO, CIÊNCIA E TECNOLOGIA DO SUL DE MINAS GERAIS; FILIPE ALMENDAGNA RODRIGUES, UNIVERSIDADE FEDERAL DE LAVRAS; MOACIR PASQUAL, UNIVERSIDADE FEDERAL DE LAVRAS; CARLOS HENRIQUE S DE CARVALHO, CNPCa.
dc.contributor.author.fl_str_mv MACIEL, A. L. de R.
RODRIGUES, F. A.
PASQUAL, M.
CARVALHO, C. H. S. de
dc.subject.por.fl_str_mv Vegetative propagation
Biotechnology
Tissue culture
Coffea arabica var. arabica
topic Vegetative propagation
Biotechnology
Tissue culture
Coffea arabica var. arabica
description The study aims to compare the efficiency of previously used liquid media for coffee, evaluating NAA concentrations in liquid medium and proline in semi-solid medium in the regeneration of somatic embryos and asses concentrations of BA and IAA in the maturation of embryos in temporary immersion bioreactors. For the regeneration of globular embryos from embryogenic aggregates and calli, we tested five concentrations (0.00, 0.25, 0.50, 1.00 and 2.00 mg L-1) of NAA in liquid medium and five concentrations (0.0, 0.5, 1.0, 2.0 and 4.0 g L-1) of proline in semisolid medium. The multiplication of embryogenic aggregates was highest in culture medium MM, reaching a density 7.5 times greater than that of the initial density. NAA promoted a linear increase in embryo regeneration. The medium containing 2.0 mg L-1 BA and 0.0 mg L-1 IAA yielded the highest percentage of large cotyledonary embryos.
publishDate 2016
dc.date.none.fl_str_mv 2016
2024-01-04T12:32:10Z
2024-01-04T12:32:10Z
2024-01-04
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Crop Breeding and Applied Biotechnology, v. 16, n. 2, p. 102-107, 2016.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1160431
http://dx.doi.org/10.1590/198470332016v16n2a16
identifier_str_mv Crop Breeding and Applied Biotechnology, v. 16, n. 2, p. 102-107, 2016.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1160431
http://dx.doi.org/10.1590/198470332016v16n2a16
dc.language.iso.fl_str_mv eng
language eng
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instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
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repository.mail.fl_str_mv cg-riaa@embrapa.br
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