Exposure to Leishmania braziliensis triggers neutrophil activation and apoptosis.

Detalhes bibliográficos
Autor(a) principal: Falcão, Sarah de Athayde Couto
Data de Publicação: 2015
Outros Autores: Weinkopff, Tiffany, Hurrell, Benjamin P, Celes, Fabiana Santana, Curvelo, Rebecca Pereira, Prates, Deboraci Brito, Barral, Aldina Maria Prado, Borges, Valeria de Matos, Cottier, Fabienne Tacchini, Oliveira, Camila Indiani de
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10576
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
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spelling Falcão, Sarah de Athayde CoutoWeinkopff, TiffanyHurrell, Benjamin PCeles, Fabiana SantanaCurvelo, Rebecca PereiraPrates, Deboraci BritoBarral, Aldina Maria PradoBorges, Valeria de MatosCottier, Fabienne TacchiniOliveira, Camila Indiani de2015-05-28T16:18:59Z2015-05-28T16:18:59Z2015FALCÃO, S. A. C. et al. Exposure to Leishmania braziliensis triggers neutrophil activation and apoptosis. PLoS Neglected Tropical Diseases, v. 9, n. 3, p. e0003601, 2015.1935-2727https://www.arca.fiocruz.br/handle/icict/1057610.1371/journal.pntd.0003601porPublic Library of ScienceNeutrófilosLeishmaniaLeishmania braziliensisApoptoseAnimaisRatosMedula ósseaInfecçãoExposure to Leishmania braziliensis triggers neutrophil activation and apoptosis.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversity of Lausanne. Department of Biochemistry and WHO-Immunology Research and Training Center. Epalinges, SwitzerlandUniversity of Lausanne. Department of Biochemistry and WHO-Immunology Research and Training Center. Epalinges, SwitzerlandFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Biomorfologia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. Salvador, BA, BrasilUniversity of Lausanne. Department of Biochemistry and WHO-Immunology Research and Training Center. Epalinges, SwitzerlandFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. Salvador, BA, BrasilBACKGROUND: Neutrophils are the first line of defense against invading pathogens and are rapidly recruited to the sites of Leishmania inoculation. During Leishmania braziliensis infection, depletion of inflammatory cells significantly increases the parasite load whereas co-inoculation of neutrophils plus L. braziliensis had an opposite effect. Moreover, the co-culture of infected macrophages and neutrophils also induced parasite killing leading us to ask how neutrophils alone respond to an L. braziliensis exposure. Herein we focused on understanding the interaction between neutrophils and L. braziliensis, exploring cell activation and apoptotic fate. METHODS AND FINDINGS: Inoculation of serum-opsonized L. braziliensis promastigotes in mice induced neutrophil accumulation in vivo, peaking at 24 h. In vitro, exposure of thyoglycollate-elicited inflammatory or bone marrow neutrophils to L. braziliensis modulated the expression of surface molecules such as CD18 and CD62L, and induced the oxidative burst. Using mCherry-expressing L. braziliensis, we determined that such effects were mainly observed in infected and not in bystander cells. Neutrophil activation following contact with L. braziliensis was also confirmed by the release of TNF-α and neutrophil elastase. Lastly, neutrophils infected with L. braziliensis but not with L. major displayed markers of early apoptosis. CONCLUSIONS: We show that L. braziliensis induces neutrophil recruitment in vivo and that neutrophils exposed to the parasite in vitro respond through activation and release of inflammatory mediators. This outcome may impact on parasite elimination, particularly at the early stages of infection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82352https://www.arca.fiocruz.br/bitstream/icict/10576/1/license.txtafbdf7d7a9bcf771a1cc7edf5f618413MD51ORIGINALFalcao SAC Exposure....pdfFalcao SAC Exposure....pdfapplication/pdf1761068https://www.arca.fiocruz.br/bitstream/icict/10576/2/Falcao%20SAC%20Exposure....pdf0f6a763ad219461db536d8b712207468MD52TEXTFalcao SAC Exposure....pdf.txtFalcao SAC Exposure....pdf.txtExtracted texttext/plain58673https://www.arca.fiocruz.br/bitstream/icict/10576/3/Falcao%20SAC%20Exposure....pdf.txt0658ce787044c524a5425afa725c63eaMD53icict/105762023-03-15 14:33:24.285oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:24Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Exposure to Leishmania braziliensis triggers neutrophil activation and apoptosis.
title Exposure to Leishmania braziliensis triggers neutrophil activation and apoptosis.
spellingShingle Exposure to Leishmania braziliensis triggers neutrophil activation and apoptosis.
Falcão, Sarah de Athayde Couto
Neutrófilos
Leishmania
Leishmania braziliensis
Apoptose
Animais
Ratos
Medula óssea
Infecção
title_short Exposure to Leishmania braziliensis triggers neutrophil activation and apoptosis.
title_full Exposure to Leishmania braziliensis triggers neutrophil activation and apoptosis.
title_fullStr Exposure to Leishmania braziliensis triggers neutrophil activation and apoptosis.
title_full_unstemmed Exposure to Leishmania braziliensis triggers neutrophil activation and apoptosis.
title_sort Exposure to Leishmania braziliensis triggers neutrophil activation and apoptosis.
author Falcão, Sarah de Athayde Couto
author_facet Falcão, Sarah de Athayde Couto
Weinkopff, Tiffany
Hurrell, Benjamin P
Celes, Fabiana Santana
Curvelo, Rebecca Pereira
Prates, Deboraci Brito
Barral, Aldina Maria Prado
Borges, Valeria de Matos
Cottier, Fabienne Tacchini
Oliveira, Camila Indiani de
author_role author
author2 Weinkopff, Tiffany
Hurrell, Benjamin P
Celes, Fabiana Santana
Curvelo, Rebecca Pereira
Prates, Deboraci Brito
Barral, Aldina Maria Prado
Borges, Valeria de Matos
Cottier, Fabienne Tacchini
Oliveira, Camila Indiani de
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Falcão, Sarah de Athayde Couto
Weinkopff, Tiffany
Hurrell, Benjamin P
Celes, Fabiana Santana
Curvelo, Rebecca Pereira
Prates, Deboraci Brito
Barral, Aldina Maria Prado
Borges, Valeria de Matos
Cottier, Fabienne Tacchini
Oliveira, Camila Indiani de
dc.subject.other.pt_BR.fl_str_mv Neutrófilos
Leishmania
Leishmania braziliensis
Apoptose
Animais
Ratos
Medula óssea
Infecção
topic Neutrófilos
Leishmania
Leishmania braziliensis
Apoptose
Animais
Ratos
Medula óssea
Infecção
description Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
publishDate 2015
dc.date.accessioned.fl_str_mv 2015-05-28T16:18:59Z
dc.date.available.fl_str_mv 2015-05-28T16:18:59Z
dc.date.issued.fl_str_mv 2015
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FALCÃO, S. A. C. et al. Exposure to Leishmania braziliensis triggers neutrophil activation and apoptosis. PLoS Neglected Tropical Diseases, v. 9, n. 3, p. e0003601, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10576
dc.identifier.issn.none.fl_str_mv 1935-2727
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0003601
identifier_str_mv FALCÃO, S. A. C. et al. Exposure to Leishmania braziliensis triggers neutrophil activation and apoptosis. PLoS Neglected Tropical Diseases, v. 9, n. 3, p. e0003601, 2015.
1935-2727
10.1371/journal.pntd.0003601
url https://www.arca.fiocruz.br/handle/icict/10576
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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