Phenotypic and functional characteristics of HLA-DR(+) neutrophils in Brazilians with cutaneous leishmaniasis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/18783 |
Resumo: | Tropical Medicine Research Center from the U.S. National Institutes of Health (Grant P50 AI-30639 to O.B., E.M.C., and M.E.W. and Grants R01 AI076233 and R01 AI045540), a Merit Review grant from the Department of Veterans’ Affairs (Grant 5I01BX001983), and a Major Project Award and Travel grant from the Stanley Foundation/University of Iowa Iowa. |
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Davis, Richard ESharma, SmritiConceição, Jacilara AlexandrinoCarneiro, Pedro Paulo OliveiraNovais, Fernanda OliveiraScott, PhillipSundar, ShyamBacellar, Maria Olívia Amado RamosCarvalho Filho, Edgar MarcelinoWilson, Mary E2017-05-08T16:47:48Z2017-05-08T16:47:48Z2017DAVIS, R. E. et al. Phenotypic and functional characteristics of HLA-DR(+) neutrophils in Brazilians with cutaneous leishmaniasis. Journal of Leukocyte Biology, v. 101, p. 739–749, 2017.0741-5400https://www.arca.fiocruz.br/handle/icict/1878310.1189/jlb.4A0915-442RRTropical Medicine Research Center from the U.S. National Institutes of Health (Grant P50 AI-30639 to O.B., E.M.C., and M.E.W. and Grants R01 AI076233 and R01 AI045540), a Merit Review grant from the Department of Veterans’ Affairs (Grant 5I01BX001983), and a Major Project Award and Travel grant from the Stanley Foundation/University of Iowa Iowa.University of Iowa. Departments of Internal Medicine and Microbiology. Iowa City, Iowa, USABanaras Hindu University. Institute of Medical Sciences. Varanasi, Uttar Pradesh, IndiaUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais. Salvador, BA, BrasilUniversity of Pennsylvania. School of Veterinary Medicine. Department of Pathobiology. Philadelphia, Pennsylvania, USAUniversity of Pennsylvania. School of Veterinary Medicine. Department of Pathobiology. Philadelphia, Pennsylvania, USABanaras Hindu University. Institute of Medical Sciences. Varanasi, Uttar Pradesh, IndiaUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilUniversity of Iowa. Departments of Internal Medicine and Microbiology. Iowa City, Iowa, USA / Iowa City Veterans’ Affairs Medical Center. Research Service. Iowa City, Iowa, USAThe protozoan Leishmania braziliensis causes cutaneous leishmaniasis (CL) in endemic regions. In murine models, neutrophils (PMNs) are recruited to the site of infection soon after parasite inoculation. However, the roles of neutrophils during chronic infection and in human disease remain undefined. We hypothesized that neutrophils help maintain a systemic inflammatory state in subjects with CL. Lesion biopsies from all patients with CL tested contained neutrophils expressing HLA-DR, a molecule thought to be restricted to professional antigen-presenting cells. Although CL is a localized disease, a subset of patients with CL also had circulating neutrophils expressing HLA-DR and the costimulatory molecules CD80, CD86, and CD40. PMNs isolated from a low-density leukocyte blood fraction (LD-PMNs) contained a higher percentage of HLA-DR(+) PMNs than did normal-density PMNs. In vitro coculture experiments suggested LD-PMNs do not suppress T cell responses, differentiating them from MDSCs. Flow-sorted HLA-DR(+) PMNs morphologically resembled conventional PMNs, and they exhibited functional properties of PMNs. Compared with conventional PMNs, HLA-DR(+) PMNs showed increased activation, degranulation, DHR123 oxidation, and phagocytic capacity. A few HLA-DR(+) PMNs were observed in healthy subjects, and that proportion could be increased by incubation in either inflammatory cytokines or in plasma from a patient with CL. This was accompanied by an increase in PMN hladrb1 mRNA, suggesting a possible connection between neutrophil "priming" and up-regulation of HLA-DR. These data suggest that PMNs that are primed for activation and that also express surface markers of antigen-presenting cells emerge in the circulation and infected tissue lesions of patients with CL.engSociety for Leukocyte BiologyLeishmaniaNeutrófiloGranulócitoLeishmaniaNeutrophilGranulocyteLeishmaniaNeutrófilosGranulócitosLeishmania braziliensisPhenotypic and functional characteristics of HLA-DR(+) neutrophils in Brazilians with cutaneous leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/18783/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALDavis RE Phenotyoic and functional....pdfDavis RE Phenotyoic and 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| dc.title.pt_BR.fl_str_mv |
Phenotypic and functional characteristics of HLA-DR(+) neutrophils in Brazilians with cutaneous leishmaniasis |
| title |
Phenotypic and functional characteristics of HLA-DR(+) neutrophils in Brazilians with cutaneous leishmaniasis |
| spellingShingle |
Phenotypic and functional characteristics of HLA-DR(+) neutrophils in Brazilians with cutaneous leishmaniasis Davis, Richard E Leishmania Neutrófilo Granulócito Leishmania Neutrophil Granulocyte Leishmania Neutrófilos Granulócitos Leishmania braziliensis |
| title_short |
Phenotypic and functional characteristics of HLA-DR(+) neutrophils in Brazilians with cutaneous leishmaniasis |
| title_full |
Phenotypic and functional characteristics of HLA-DR(+) neutrophils in Brazilians with cutaneous leishmaniasis |
| title_fullStr |
Phenotypic and functional characteristics of HLA-DR(+) neutrophils in Brazilians with cutaneous leishmaniasis |
| title_full_unstemmed |
Phenotypic and functional characteristics of HLA-DR(+) neutrophils in Brazilians with cutaneous leishmaniasis |
| title_sort |
Phenotypic and functional characteristics of HLA-DR(+) neutrophils in Brazilians with cutaneous leishmaniasis |
| author |
Davis, Richard E |
| author_facet |
Davis, Richard E Sharma, Smriti Conceição, Jacilara Alexandrino Carneiro, Pedro Paulo Oliveira Novais, Fernanda Oliveira Scott, Phillip Sundar, Shyam Bacellar, Maria Olívia Amado Ramos Carvalho Filho, Edgar Marcelino Wilson, Mary E |
| author_role |
author |
| author2 |
Sharma, Smriti Conceição, Jacilara Alexandrino Carneiro, Pedro Paulo Oliveira Novais, Fernanda Oliveira Scott, Phillip Sundar, Shyam Bacellar, Maria Olívia Amado Ramos Carvalho Filho, Edgar Marcelino Wilson, Mary E |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Davis, Richard E Sharma, Smriti Conceição, Jacilara Alexandrino Carneiro, Pedro Paulo Oliveira Novais, Fernanda Oliveira Scott, Phillip Sundar, Shyam Bacellar, Maria Olívia Amado Ramos Carvalho Filho, Edgar Marcelino Wilson, Mary E |
| dc.subject.other.pt_BR.fl_str_mv |
Leishmania Neutrófilo Granulócito |
| topic |
Leishmania Neutrófilo Granulócito Leishmania Neutrophil Granulocyte Leishmania Neutrófilos Granulócitos Leishmania braziliensis |
| dc.subject.en.pt_BR.fl_str_mv |
Leishmania Neutrophil Granulocyte |
| dc.subject.decs.pt_BR.fl_str_mv |
Leishmania Neutrófilos Granulócitos Leishmania braziliensis |
| description |
Tropical Medicine Research Center from the U.S. National Institutes of Health (Grant P50 AI-30639 to O.B., E.M.C., and M.E.W. and Grants R01 AI076233 and R01 AI045540), a Merit Review grant from the Department of Veterans’ Affairs (Grant 5I01BX001983), and a Major Project Award and Travel grant from the Stanley Foundation/University of Iowa Iowa. |
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2017 |
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2017-05-08T16:47:48Z |
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2017-05-08T16:47:48Z |
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2017 |
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DAVIS, R. E. et al. Phenotypic and functional characteristics of HLA-DR(+) neutrophils in Brazilians with cutaneous leishmaniasis. Journal of Leukocyte Biology, v. 101, p. 739–749, 2017. |
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https://www.arca.fiocruz.br/handle/icict/18783 |
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0741-5400 |
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10.1189/jlb.4A0915-442RR |
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DAVIS, R. E. et al. Phenotypic and functional characteristics of HLA-DR(+) neutrophils in Brazilians with cutaneous leishmaniasis. Journal of Leukocyte Biology, v. 101, p. 739–749, 2017. 0741-5400 10.1189/jlb.4A0915-442RR |
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Society for Leukocyte Biology |
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