Gene Expression Profile of High IFN-γ Producers Stimulated with Leishmania braziliensis Identifies Genes Associated with Cutaneous Leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Carneiro, Marcia Weber
Data de Publicação: 2016
Outros Autores: Fukutani, Kiyoshi Ferreira, Andrade, Bruno de Bezerril, Curvelo, Rebecca Pereira, Cristal, Juqueline Rocha, Carvalho, Augusto Marcelino Pedreira de, Barral, Aldina Maria Prado, Van Weyenbergh, Johan Jozef Rosa Maria, Barral Netto, Manoel, Oliveira, Camila Indiani de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/18109
Resumo: Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq; grant 302464/2009-3 to M.BN and fellowship to MWC and KFF), Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB) (fellowship to KFF) and Coordenação de Apoio à Pesquisa e Ensino Superior (CAPES)
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spelling Carneiro, Marcia WeberFukutani, Kiyoshi FerreiraAndrade, Bruno de BezerrilCurvelo, Rebecca PereiraCristal, Juqueline RochaCarvalho, Augusto Marcelino Pedreira deBarral, Aldina Maria PradoVan Weyenbergh, Johan Jozef Rosa MariaBarral Netto, ManoelOliveira, Camila Indiani de2017-03-21T18:00:06Z2017-03-21T18:00:06Z2016Gene Expression Profile of High IFN-γ CARNEIRO, M. W. et al. Producers Stimulated with Leishmania braziliensis Identifies Genes Associated with Cutaneous Leishmaniasis. PLoS Neglected Tropical Diseases, v. 10, n. 11, p. e0005116, 2016.1935-2727https://www.arca.fiocruz.br/handle/icict/1810910.1371/journal.pntd.0005116Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq; grant 302464/2009-3 to M.BN and fellowship to MWC and KFF), Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB) (fellowship to KFF) and Coordenação de Apoio à Pesquisa e Ensino Superior (CAPES)Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Fundação Jose Silveira. Multinational Organization Network Sponsoring Translational and Epidemiological Research (MONSTER) Initiative. Salvador, BA, Brasil,Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. School of Medicine. Salvador, BA, BrasilRega Institute for Medical Research. Department of Microbiology and Immunology. KU Leuven, Leuven, BelgiumFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. School of Medicine. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilThe initial response to Leishmania parasites is essential in determining disease development or resistance. In vitro, a divergent response to Leishmania, characterized by high or low IFN-γ production has been described as a potential tool to predict both vaccine response and disease susceptibility in vivo. Methods and findings We identified uninfected and healthy individuals that were shown to be either high- or low IFN-γ producers (HPs and LPs, respectively) following stimulation of peripheral blood cells with Leishmania braziliensis. Following stimulation, RNA was processed for gene expression analysis using immune gene arrays. Both HPs and LPs were shown to upregulate the expression of CXCL10, IFI27, IL6 and LTA. Genes expressed in HPs only (CCL7, IL8, IFI44L and IL1B) were associated with pathways related to IL17 and TREM 1 signaling. In LPs, uniquely expressed genes (for example IL9, IFI44, IFIT1 and IL2RA) were associated with pathways related to pattern recognition receptors and interferon signaling. We then investigated whether the unique gene expression profiles described here could be recapitulated in vivo, in individuals with active Cutaneous Leishmaniasis or with subclinical infection. Indeed, using a set of six genes (TLR2, JAK2, IFI27, IFIT1, IRF1 and IL6) modulated in HPs and LPs, we could successfully discriminate these two clinical groups. Finally, we demonstrate that these six genes are significantly overexpressed in CL lesions. Conclusion Upon interrogation of the peripheral response of naive individuals with diverging IFN-γ production to L. braziliensis, we identified differences in the innate response to the parasite that are recapitulated in vivo and that discriminate CL patients from individuals presenting a subclinical infectionengPublic Library of ScienceLeishmania braziliensisLeishmaniose cutâneaGenéticaVacinaInfecçãoLeishmania braziliensisCutaneous leishmaniasisGeneticVaccineGene Expression Profile of High IFN-γ Producers Stimulated with Leishmania braziliensis Identifies Genes Associated with Cutaneous Leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/18109/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALCarneiro MW Gene 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dc.title.pt_BR.fl_str_mv Gene Expression Profile of High IFN-γ Producers Stimulated with Leishmania braziliensis Identifies Genes Associated with Cutaneous Leishmaniasis
title Gene Expression Profile of High IFN-γ Producers Stimulated with Leishmania braziliensis Identifies Genes Associated with Cutaneous Leishmaniasis
spellingShingle Gene Expression Profile of High IFN-γ Producers Stimulated with Leishmania braziliensis Identifies Genes Associated with Cutaneous Leishmaniasis
Carneiro, Marcia Weber
Leishmania braziliensis
Leishmaniose cutânea
Genética
Vacina
Infecção
Leishmania braziliensis
Cutaneous leishmaniasis
Genetic
Vaccine
title_short Gene Expression Profile of High IFN-γ Producers Stimulated with Leishmania braziliensis Identifies Genes Associated with Cutaneous Leishmaniasis
title_full Gene Expression Profile of High IFN-γ Producers Stimulated with Leishmania braziliensis Identifies Genes Associated with Cutaneous Leishmaniasis
title_fullStr Gene Expression Profile of High IFN-γ Producers Stimulated with Leishmania braziliensis Identifies Genes Associated with Cutaneous Leishmaniasis
title_full_unstemmed Gene Expression Profile of High IFN-γ Producers Stimulated with Leishmania braziliensis Identifies Genes Associated with Cutaneous Leishmaniasis
title_sort Gene Expression Profile of High IFN-γ Producers Stimulated with Leishmania braziliensis Identifies Genes Associated with Cutaneous Leishmaniasis
author Carneiro, Marcia Weber
author_facet Carneiro, Marcia Weber
Fukutani, Kiyoshi Ferreira
Andrade, Bruno de Bezerril
Curvelo, Rebecca Pereira
Cristal, Juqueline Rocha
Carvalho, Augusto Marcelino Pedreira de
Barral, Aldina Maria Prado
Van Weyenbergh, Johan Jozef Rosa Maria
Barral Netto, Manoel
Oliveira, Camila Indiani de
author_role author
author2 Fukutani, Kiyoshi Ferreira
Andrade, Bruno de Bezerril
Curvelo, Rebecca Pereira
Cristal, Juqueline Rocha
Carvalho, Augusto Marcelino Pedreira de
Barral, Aldina Maria Prado
Van Weyenbergh, Johan Jozef Rosa Maria
Barral Netto, Manoel
Oliveira, Camila Indiani de
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Carneiro, Marcia Weber
Fukutani, Kiyoshi Ferreira
Andrade, Bruno de Bezerril
Curvelo, Rebecca Pereira
Cristal, Juqueline Rocha
Carvalho, Augusto Marcelino Pedreira de
Barral, Aldina Maria Prado
Van Weyenbergh, Johan Jozef Rosa Maria
Barral Netto, Manoel
Oliveira, Camila Indiani de
dc.subject.other.pt_BR.fl_str_mv Leishmania braziliensis
Leishmaniose cutânea
Genética
Vacina
Infecção
topic Leishmania braziliensis
Leishmaniose cutânea
Genética
Vacina
Infecção
Leishmania braziliensis
Cutaneous leishmaniasis
Genetic
Vaccine
dc.subject.en.pt_BR.fl_str_mv Leishmania braziliensis
Cutaneous leishmaniasis
Genetic
Vaccine
description Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq; grant 302464/2009-3 to M.BN and fellowship to MWC and KFF), Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB) (fellowship to KFF) and Coordenação de Apoio à Pesquisa e Ensino Superior (CAPES)
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-03-21T18:00:06Z
dc.date.available.fl_str_mv 2017-03-21T18:00:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv Gene Expression Profile of High IFN-γ CARNEIRO, M. W. et al. Producers Stimulated with Leishmania braziliensis Identifies Genes Associated with Cutaneous Leishmaniasis. PLoS Neglected Tropical Diseases, v. 10, n. 11, p. e0005116, 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/18109
dc.identifier.issn.pt_BR.fl_str_mv 1935-2727
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0005116
identifier_str_mv Gene Expression Profile of High IFN-γ CARNEIRO, M. W. et al. Producers Stimulated with Leishmania braziliensis Identifies Genes Associated with Cutaneous Leishmaniasis. PLoS Neglected Tropical Diseases, v. 10, n. 11, p. e0005116, 2016.
1935-2727
10.1371/journal.pntd.0005116
url https://www.arca.fiocruz.br/handle/icict/18109
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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