Targeting the Non-structural Protein 1 from Dengue Virus to a Dendritic Cell Population Confers Protective Immunity to Lethal Virus Challenge

Detalhes bibliográficos
Autor(a) principal: Henriques, Hugo R.
Data de Publicação: 2013
Outros Autores: Rampazo, Eline V., Gonçalves, Antonio J. S., Vicentin, Elaine C. M., Amorim, Jaime H., Panatieri, Raquel H., Amorim, Kelly N. S., Yamamoto, Marcio M., Ferreira, Luís C. S., Alves, Ada M. B., Boscardin, Silvia B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/11458
Resumo: Universidade de São Paulo. Departamento de Parasitologia. Laboratório de Direcionamento de Antígenos para Células Dendríticas. São Paulo, SP, Brasil.
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spelling Henriques, Hugo R.Rampazo, Eline V.Gonçalves, Antonio J. S.Vicentin, Elaine C. M.Amorim, Jaime H.Panatieri, Raquel H.Amorim, Kelly N. S.Yamamoto, Marcio M.Ferreira, Luís C. S.Alves, Ada M. B.Boscardin, Silvia B.2015-08-19T13:49:12Z2015-08-19T13:49:12Z2013HENRIQUES, Hugo R.; et al. Targeting the Non-structural Protein 1 from Dengue Virus to a Dendritic Cell Population Confers Protective Immunity to Lethal Virus Challenge. PLoS Negl Trop Dis, v.7, n.7, e2330, 13p, July 2013.https://www.arca.fiocruz.br/handle/icict/1145810.1371/journal.pntd.0002330engPlos OneTargeting the Non-structural Protein 1 from Dengue Virus to a Dendritic Cell Population Confers Protective Immunity to Lethal Virus Challengeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade de São Paulo. Departamento de Parasitologia. Laboratório de Direcionamento de Antígenos para Células Dendríticas. São Paulo, SP, Brasil.Universidade de São Paulo. Departamento de Parasitologia. Laboratório de Direcionamento de Antígenos para Células Dendríticas. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biotecnologia e Fisiologia de Infecções Virais. Rio de Janeiro, RJ, Brasil.Universidade de São Paulo. Departamento de Parasitologia. Laboratório de Direcionamento de Antígenos para Células Dendríticas. São Paulo, SP, Brasil.Universidade de São Paulo. Departamento de Microbiologia. Laboratório de Desenvolvimento de Vacinas. São Paulo, SP, Brasil.Universidade de São Paulo. Departamento de Parasitologia. Laboratório de Direcionamento de Antígenos para Células Dendríticas. São Paulo, SP, Brasil.Universidade de São Paulo. Departamento de Parasitologia. Laboratório de Direcionamento de Antígenos para Células Dendríticas. São Paulo, SP, Brasil.Universidade de São Paulo. Departamento de Parasitologia. Laboratório de Direcionamento de Antígenos para Células Dendríticas. São Paulo, SP, Brasil.Universidade de São Paulo. Departamento de Microbiologia. Laboratório de Desenvolvimento de Vacinas. São Paulo, SP, Brasil / Instituto Nacional de Ciência e Tecnologia em Vacinas. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biotecnologia e Fisiologia de Infecções Virais. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Vacinas. Belo Horizonte, MG, Brasil.Universidade de São Paulo. Departamento de Parasitologia. Laboratório de Direcionamento de Antígenos para Células Dendríticas. São Paulo, SP, Brasil / Instituto Nacional de Ciência e Tecnologia em Vacinas. Belo Horizonte, MG, Brasil.Dengue is the most prevalent arboviral infection, affecting millions of people every year. Attempts to control such infection are being made, and the development of a vaccine is a World Health Organization priority. Among the proteins being tested as vaccine candidates in preclinical settings is the non-structural protein 1 (NS1). In the present study, we tested the immune responses generated by targeting the NS1 protein to two different dendritic cell populations. Dendritic cells (DCs) are important antigen presenting cells, and targeting proteins to maturing DCs has proved to be an efficient means of immunization. Antigen targeting is accomplished by the use of a monoclonal antibody (mAb) directed against a DC cell surface receptor fused to the protein of interest. We used two mAbs (aDEC205 and aDCIR2) to target two distinct DC populations, expressing either DEC205 or DCIR2 endocytic receptors, respectively, in mice. The fusion mAbs were successfully produced, bound to their respective receptors, and were used to immunize BALB/c mice in the presence of polyriboinosinic: polyribocytidylic acid (poly (I:C)), as a DC maturation stimulus. We observed induction of strong anti-NS1 antibody responses and similar antigen binding affinity irrespectively of the DC population targeted. Nevertheless, the IgG1/ IgG2a ratios were different between mouse groups immunized with aDEC-NS1 and aDCIR2-NS1 mAbs. When we tested the induction of cellular immune responses, the number of IFN-c producing cells was higher in aDEC-NS1 immunized animals. In addition, mice immunized with the aDEC-NS1 mAb were significantly protected from a lethal intracranial challenge with the DENV2 NGC strain when compared to mice immunized with aDCIR2-NS1 mAb. Protection was partially mediated by CD4+ and CD8+ T cells as depletion of these populations reduced both survival and morbidity signs. We conclude that targeting the NS1 protein to the DEC205+ DC population with poly (I:C) opens perspectives for dengue vaccine development.DengueDendritic CellNon-structural Protein 1DengueCélulas Dendríticasinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/11458/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALada_alves_etal_IOC_2013.pdfapplication/pdf1495925https://www.arca.fiocruz.br/bitstream/icict/11458/2/ada_alves_etal_IOC_2013.pdf2256c2cca71008f6696fcdf085ccddb6MD52TEXTada_alves_etal_IOC_2013.pdf.txtada_alves_etal_IOC_2013.pdf.txtExtracted texttext/plain66182https://www.arca.fiocruz.br/bitstream/icict/11458/3/ada_alves_etal_IOC_2013.pdf.txt9581662b8d1c765e134397b0e29166acMD53icict/114582023-09-05 10:23:00.894oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-05T13:23Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Targeting the Non-structural Protein 1 from Dengue Virus to a Dendritic Cell Population Confers Protective Immunity to Lethal Virus Challenge
title Targeting the Non-structural Protein 1 from Dengue Virus to a Dendritic Cell Population Confers Protective Immunity to Lethal Virus Challenge
spellingShingle Targeting the Non-structural Protein 1 from Dengue Virus to a Dendritic Cell Population Confers Protective Immunity to Lethal Virus Challenge
Henriques, Hugo R.
Dengue
Dendritic Cell
Non-structural Protein 1
Dengue
Células Dendríticas
title_short Targeting the Non-structural Protein 1 from Dengue Virus to a Dendritic Cell Population Confers Protective Immunity to Lethal Virus Challenge
title_full Targeting the Non-structural Protein 1 from Dengue Virus to a Dendritic Cell Population Confers Protective Immunity to Lethal Virus Challenge
title_fullStr Targeting the Non-structural Protein 1 from Dengue Virus to a Dendritic Cell Population Confers Protective Immunity to Lethal Virus Challenge
title_full_unstemmed Targeting the Non-structural Protein 1 from Dengue Virus to a Dendritic Cell Population Confers Protective Immunity to Lethal Virus Challenge
title_sort Targeting the Non-structural Protein 1 from Dengue Virus to a Dendritic Cell Population Confers Protective Immunity to Lethal Virus Challenge
author Henriques, Hugo R.
author_facet Henriques, Hugo R.
Rampazo, Eline V.
Gonçalves, Antonio J. S.
Vicentin, Elaine C. M.
Amorim, Jaime H.
Panatieri, Raquel H.
Amorim, Kelly N. S.
Yamamoto, Marcio M.
Ferreira, Luís C. S.
Alves, Ada M. B.
Boscardin, Silvia B.
author_role author
author2 Rampazo, Eline V.
Gonçalves, Antonio J. S.
Vicentin, Elaine C. M.
Amorim, Jaime H.
Panatieri, Raquel H.
Amorim, Kelly N. S.
Yamamoto, Marcio M.
Ferreira, Luís C. S.
Alves, Ada M. B.
Boscardin, Silvia B.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Henriques, Hugo R.
Rampazo, Eline V.
Gonçalves, Antonio J. S.
Vicentin, Elaine C. M.
Amorim, Jaime H.
Panatieri, Raquel H.
Amorim, Kelly N. S.
Yamamoto, Marcio M.
Ferreira, Luís C. S.
Alves, Ada M. B.
Boscardin, Silvia B.
dc.subject.en.pt_BR.fl_str_mv Dengue
Dendritic Cell
Non-structural Protein 1
topic Dengue
Dendritic Cell
Non-structural Protein 1
Dengue
Células Dendríticas
dc.subject.decs.pt_BR.fl_str_mv Dengue
Células Dendríticas
description Universidade de São Paulo. Departamento de Parasitologia. Laboratório de Direcionamento de Antígenos para Células Dendríticas. São Paulo, SP, Brasil.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2015-08-19T13:49:12Z
dc.date.available.fl_str_mv 2015-08-19T13:49:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv HENRIQUES, Hugo R.; et al. Targeting the Non-structural Protein 1 from Dengue Virus to a Dendritic Cell Population Confers Protective Immunity to Lethal Virus Challenge. PLoS Negl Trop Dis, v.7, n.7, e2330, 13p, July 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/11458
dc.identifier.doi.pt_BR.fl_str_mv 10.1371/journal.pntd.0002330
identifier_str_mv HENRIQUES, Hugo R.; et al. Targeting the Non-structural Protein 1 from Dengue Virus to a Dendritic Cell Population Confers Protective Immunity to Lethal Virus Challenge. PLoS Negl Trop Dis, v.7, n.7, e2330, 13p, July 2013.
10.1371/journal.pntd.0002330
url https://www.arca.fiocruz.br/handle/icict/11458
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Plos One
publisher.none.fl_str_mv Plos One
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/11458/1/license.txt
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