Unveiling the partners of the DRBD2-mRNP complex, an RBP in Trypanosoma cruzi and ortholog to the yeast SR-protein Gbp2

Detalhes bibliográficos
Autor(a) principal: Wippel, Helisa Helena
Data de Publicação: 2019
Outros Autores: Malgarin, Juliane Soldi, Inoue, Alexandre Haruo, Leprevost, Felipe da Veiga, Carvalho, Paulo Costa, Goldenberg, Samuel, Alves, Lysangela Ronalte
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/34842
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas.Curitiba, PR, Brasil.
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spelling Wippel, Helisa HelenaMalgarin, Juliane SoldiInoue, Alexandre HaruoLeprevost, Felipe da VeigaCarvalho, Paulo CostaGoldenberg, SamuelAlves, Lysangela Ronalte2019-08-15T19:23:11Z2019-08-15T19:23:11Z2019WIPPEL, Helisa Helena et al. Unveiling the partners of the DRBD2-mRNP complex, an RBP in Trypanosoma cruzi and ortholog to the yeast SR-protein Gbp2. BMC Microbiology, v. 19, n. 128, p. 1-12, 2019.1471-2180https://www.arca.fiocruz.br/handle/icict/3484210.1186/s12866-019-1505-81471-2180porBMCMetabolismo de RNATrypanosoma cruziRNA-Binding ProteinsGene Expression RegulationRNAProteínas de Unión al ARNRegulación de la Expresión GénicaARNProteínas de Ligação a RNARegulação da Expressão GênicaRNAUnveiling the partners of the DRBD2-mRNP complex, an RBP in Trypanosoma cruzi and ortholog to the yeast SR-protein Gbp2info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas.Curitiba, PR, Brasil.Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas.Curitiba, PR, Brasil / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Medical Science Unit I. Department of Pathology. University of Michigan. Michigan, USA.Fundação Oswaldo Cruz. Instituto Carlos Chagas.Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas.Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.RNA-binding proteins (RBPs) are well known as key factors in gene expression regulation in eukaryotes. These proteins associate with mRNAs and other proteins to form mRNP complexes that ultimately determine the fate of target transcripts in the cell. This association is usually mediated by an RNA-recognition motif (RRM). In the case of trypanosomatids, these proteins play a paramount role, as gene expression regulation is mostly posttranscriptional. Despite their relevance in the life cycle of Trypanosoma cruzi, the causative agent of Chagas’ disease, to date, few RBPs have been characterized in this parasite. And as results , we investigated the role of DRBD2 in T. cruzi, an RBP with two RRM domains that is associated with cytoplasmic translational complexes. We show that DRBD2 is an ortholog of the Gbp2 in yeast, an SR-rich protein involved in mRNA quality control and export. We used an immunoprecipitation assay followed by shotgun proteomics and RNA-seq to assess the interaction partners of the DRBD2-mRNP complex in epimastigotes. The analysis identified mostly proteins involved in RNA metabolism and regulation, such as ALBA1, ALBA3, ALBA4, UBP1, UBP2, DRBD3, and PABP2. The RNA-seq results showed that most of the transcripts regulated by the DRBD2 complex mapped to hypothetical proteins related to multiple processes, such as to biosynthetic process, DNA metabolic process, protein modification, and response to stress. Thus, It concluded that, the identification of regulatory proteins in the DRBD2-mRNP complex corroborates the important role of DRBD2 in gene expression regulation in T. cruzi. We consider these results an important contribution to future studies regarding gene expression regulation in T. cruzi, especially in the field of RNA-binding proteins.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/34842/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALs12866-019-1505.pdfs12866-019-1505.pdfapplication/pdf2567810https://www.arca.fiocruz.br/bitstream/icict/34842/2/s12866-019-1505.pdf8cedb9c28885f4fc96904def1459961fMD52TEXTs12866-019-1505.pdf.txts12866-019-1505.pdf.txtExtracted texttext/plain53784https://www.arca.fiocruz.br/bitstream/icict/34842/3/s12866-019-1505.pdf.txt6b76a3c3b65c7dbcd09e0cd0b5cb1656MD53icict/348422019-08-16 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dc.title.pt_BR.fl_str_mv Unveiling the partners of the DRBD2-mRNP complex, an RBP in Trypanosoma cruzi and ortholog to the yeast SR-protein Gbp2
title Unveiling the partners of the DRBD2-mRNP complex, an RBP in Trypanosoma cruzi and ortholog to the yeast SR-protein Gbp2
spellingShingle Unveiling the partners of the DRBD2-mRNP complex, an RBP in Trypanosoma cruzi and ortholog to the yeast SR-protein Gbp2
Wippel, Helisa Helena
Metabolismo de RNA
Trypanosoma cruzi
RNA-Binding Proteins
Gene Expression Regulation
RNA
Proteínas de Unión al ARN
Regulación de la Expresión Génica
ARN
Proteínas de Ligação a RNA
Regulação da Expressão Gênica
RNA
title_short Unveiling the partners of the DRBD2-mRNP complex, an RBP in Trypanosoma cruzi and ortholog to the yeast SR-protein Gbp2
title_full Unveiling the partners of the DRBD2-mRNP complex, an RBP in Trypanosoma cruzi and ortholog to the yeast SR-protein Gbp2
title_fullStr Unveiling the partners of the DRBD2-mRNP complex, an RBP in Trypanosoma cruzi and ortholog to the yeast SR-protein Gbp2
title_full_unstemmed Unveiling the partners of the DRBD2-mRNP complex, an RBP in Trypanosoma cruzi and ortholog to the yeast SR-protein Gbp2
title_sort Unveiling the partners of the DRBD2-mRNP complex, an RBP in Trypanosoma cruzi and ortholog to the yeast SR-protein Gbp2
author Wippel, Helisa Helena
author_facet Wippel, Helisa Helena
Malgarin, Juliane Soldi
Inoue, Alexandre Haruo
Leprevost, Felipe da Veiga
Carvalho, Paulo Costa
Goldenberg, Samuel
Alves, Lysangela Ronalte
author_role author
author2 Malgarin, Juliane Soldi
Inoue, Alexandre Haruo
Leprevost, Felipe da Veiga
Carvalho, Paulo Costa
Goldenberg, Samuel
Alves, Lysangela Ronalte
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Wippel, Helisa Helena
Malgarin, Juliane Soldi
Inoue, Alexandre Haruo
Leprevost, Felipe da Veiga
Carvalho, Paulo Costa
Goldenberg, Samuel
Alves, Lysangela Ronalte
dc.subject.other.pt_BR.fl_str_mv Metabolismo de RNA
Trypanosoma cruzi
topic Metabolismo de RNA
Trypanosoma cruzi
RNA-Binding Proteins
Gene Expression Regulation
RNA
Proteínas de Unión al ARN
Regulación de la Expresión Génica
ARN
Proteínas de Ligação a RNA
Regulação da Expressão Gênica
RNA
dc.subject.en.pt_BR.fl_str_mv RNA-Binding Proteins
Gene Expression Regulation
RNA
dc.subject.es.pt_BR.fl_str_mv Proteínas de Unión al ARN
Regulación de la Expresión Génica
ARN
dc.subject.decs.pt_BR.fl_str_mv Proteínas de Ligação a RNA
Regulação da Expressão Gênica
RNA
description Fundação Oswaldo Cruz. Instituto Carlos Chagas.Curitiba, PR, Brasil.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-08-15T19:23:11Z
dc.date.available.fl_str_mv 2019-08-15T19:23:11Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv WIPPEL, Helisa Helena et al. Unveiling the partners of the DRBD2-mRNP complex, an RBP in Trypanosoma cruzi and ortholog to the yeast SR-protein Gbp2. BMC Microbiology, v. 19, n. 128, p. 1-12, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/34842
dc.identifier.issn.pt_BR.fl_str_mv 1471-2180
dc.identifier.doi.none.fl_str_mv 10.1186/s12866-019-1505-8
dc.identifier.eissn.none.fl_str_mv 1471-2180
identifier_str_mv WIPPEL, Helisa Helena et al. Unveiling the partners of the DRBD2-mRNP complex, an RBP in Trypanosoma cruzi and ortholog to the yeast SR-protein Gbp2. BMC Microbiology, v. 19, n. 128, p. 1-12, 2019.
1471-2180
10.1186/s12866-019-1505-8
url https://www.arca.fiocruz.br/handle/icict/34842
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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institution FIOCRUZ
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