Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies

Detalhes bibliográficos
Autor(a) principal: Paiva, Marcelo Henrique Santos
Data de Publicação: 2017
Outros Autores: Guedes, Duschinka Ribeiro Duarte, Leal, Walter Soares, Ayres, Constância Flávia Junqueira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/19419
Resumo: Universidade Federal de Pernambuco. Centro Acadêmico do Agreste. Caruaru, PE, Brazil / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.
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spelling Paiva, Marcelo Henrique SantosGuedes, Duschinka Ribeiro DuarteLeal, Walter SoaresAyres, Constância Flávia Junqueira2017-06-19T19:36:01Z2017-06-19T19:36:01Z2017PAIVA, M. H. S. et al. Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies. Genetics and Molecular Biology, v. 40, n. 3, p. 597–599, 22 maio 2017.1415-4757https://www.arca.fiocruz.br/handle/icict/1941910.1590/1678-4685-GMB-2016-0312engSensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studiesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Pernambuco. Centro Acadêmico do Agreste. Caruaru, PE, Brazil / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Universidade Federal de Pernambuco. Centro Acadêmico do Agreste. Caruaru, PE, Brazil.University of California-Davis. Department of Molecular and Cellular Biology. Davis, CA, USA.Universidade Federal de Pernambuco. Centro Acadêmico do Agreste. Caruaru, PE, Brazil.Tissue samples from mosquitoes artificially infected with Zika virus and shown to be positive by RT-qPCR were reexamined by RT-PCR. Using these samples we compared the two methods employed in virus RNA detection for vector competence studies. Results demonstrated that, albeit useful, RT-PCR gave false negatives with low viral loads (< 106 RNA copies/ml).ZIKVRT-PCRAedes aegyptiVector competenceinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/19419/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINAL28534930 2017 pai-sen.oa.pdf28534930 2017 pai-sen.oa.pdfapplication/pdf584338https://www.arca.fiocruz.br/bitstream/icict/19419/2/28534930%202017%20pai-sen.oa.pdf8d6cf9195aca582f536010d665b450e4MD52TEXT28534930 2017 pai-sen.oa.pdf.txt28534930 2017 pai-sen.oa.pdf.txtExtracted texttext/plain14811https://www.arca.fiocruz.br/bitstream/icict/19419/3/28534930%202017%20pai-sen.oa.pdf.txt996374393694ae2d0b94b02fb8ef1a50MD53icict/194192019-04-02 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dc.title.pt_BR.fl_str_mv Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
title Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
spellingShingle Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
Paiva, Marcelo Henrique Santos
ZIKV
RT-PCR
Aedes aegypti
Vector competence
title_short Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
title_full Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
title_fullStr Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
title_full_unstemmed Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
title_sort Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
author Paiva, Marcelo Henrique Santos
author_facet Paiva, Marcelo Henrique Santos
Guedes, Duschinka Ribeiro Duarte
Leal, Walter Soares
Ayres, Constância Flávia Junqueira
author_role author
author2 Guedes, Duschinka Ribeiro Duarte
Leal, Walter Soares
Ayres, Constância Flávia Junqueira
author2_role author
author
author
dc.contributor.author.fl_str_mv Paiva, Marcelo Henrique Santos
Guedes, Duschinka Ribeiro Duarte
Leal, Walter Soares
Ayres, Constância Flávia Junqueira
dc.subject.en.none.fl_str_mv ZIKV
RT-PCR
Aedes aegypti
Vector competence
topic ZIKV
RT-PCR
Aedes aegypti
Vector competence
description Universidade Federal de Pernambuco. Centro Acadêmico do Agreste. Caruaru, PE, Brazil / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-06-19T19:36:01Z
dc.date.available.fl_str_mv 2017-06-19T19:36:01Z
dc.date.issued.fl_str_mv 2017
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PAIVA, M. H. S. et al. Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies. Genetics and Molecular Biology, v. 40, n. 3, p. 597–599, 22 maio 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/19419
dc.identifier.issn.pt_BR.fl_str_mv 1415-4757
dc.identifier.doi.pt_BR.fl_str_mv 10.1590/1678-4685-GMB-2016-0312
identifier_str_mv PAIVA, M. H. S. et al. Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies. Genetics and Molecular Biology, v. 40, n. 3, p. 597–599, 22 maio 2017.
1415-4757
10.1590/1678-4685-GMB-2016-0312
url https://www.arca.fiocruz.br/handle/icict/19419
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