Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies

Detalhes bibliográficos
Autor(a) principal: Paiva,Marcelo Henrique Santos
Data de Publicação: 2017
Outros Autores: Guedes,Duschinka Ribeiro Duarte, Leal,Walter Soares, Ayres,Constância Flávia Junqueira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400597
Resumo: Abstract Tissue samples from mosquitoes artificially infected with Zika virus and shown to be positive by RT-qPCR were reexamined by RT-PCR. Using these samples we compared the two methods employed in virus RNA detection for vector competence studies. Results demonstrated that, albeit useful, RT-PCR gave false negatives with low viral loads (< 106 RNA copies/ml).
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spelling Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studiesZIKVRT-PCRAedes aegyptivector competenceAbstract Tissue samples from mosquitoes artificially infected with Zika virus and shown to be positive by RT-qPCR were reexamined by RT-PCR. Using these samples we compared the two methods employed in virus RNA detection for vector competence studies. Results demonstrated that, albeit useful, RT-PCR gave false negatives with low viral loads (< 106 RNA copies/ml).Sociedade Brasileira de Genética2017-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400597Genetics and Molecular Biology v.40 n.3 2017reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/1678-4685-gmb-2016-0312info:eu-repo/semantics/openAccessPaiva,Marcelo Henrique SantosGuedes,Duschinka Ribeiro DuarteLeal,Walter SoaresAyres,Constância Flávia Junqueiraeng2017-09-01T00:00:00Zoai:scielo:S1415-47572017000400597Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2017-09-01T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
title Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
spellingShingle Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
Paiva,Marcelo Henrique Santos
ZIKV
RT-PCR
Aedes aegypti
vector competence
title_short Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
title_full Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
title_fullStr Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
title_full_unstemmed Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
title_sort Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
author Paiva,Marcelo Henrique Santos
author_facet Paiva,Marcelo Henrique Santos
Guedes,Duschinka Ribeiro Duarte
Leal,Walter Soares
Ayres,Constância Flávia Junqueira
author_role author
author2 Guedes,Duschinka Ribeiro Duarte
Leal,Walter Soares
Ayres,Constância Flávia Junqueira
author2_role author
author
author
dc.contributor.author.fl_str_mv Paiva,Marcelo Henrique Santos
Guedes,Duschinka Ribeiro Duarte
Leal,Walter Soares
Ayres,Constância Flávia Junqueira
dc.subject.por.fl_str_mv ZIKV
RT-PCR
Aedes aegypti
vector competence
topic ZIKV
RT-PCR
Aedes aegypti
vector competence
description Abstract Tissue samples from mosquitoes artificially infected with Zika virus and shown to be positive by RT-qPCR were reexamined by RT-PCR. Using these samples we compared the two methods employed in virus RNA detection for vector competence studies. Results demonstrated that, albeit useful, RT-PCR gave false negatives with low viral loads (< 106 RNA copies/ml).
publishDate 2017
dc.date.none.fl_str_mv 2017-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400597
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400597
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-4685-gmb-2016-0312
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.40 n.3 2017
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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