Characterization of virulence of Leptospira isolates in a hamster model.

Detalhes bibliográficos
Autor(a) principal: Silva, Éverton Fagonde
Data de Publicação: 2008
Outros Autores: Santos, Cleiton Silva, Athanazio, Daniel Abensur, Seyffert, Núbia, Seixas, Fabiana K, Cerqueira, Gustavo Maia de, Fagundes, Michel Q, Brod, Claudiomar Soares, Reis, Mitermayer Galvão dos, Dellagostin, Odir Antonio, Ko, Albert Icksang
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7532
Resumo: Universidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
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spelling Silva, Éverton FagondeSantos, Cleiton SilvaAthanazio, Daniel AbensurSeyffert, NúbiaSeixas, Fabiana KCerqueira, Gustavo Maia deFagundes, Michel QBrod, Claudiomar SoaresReis, Mitermayer Galvão dosDellagostin, Odir AntonioKo, Albert Icksang2014-04-24T13:23:13Z2014-04-24T13:23:13Z2008SILVA, E. F. et al. Characterization of virulence of Leptospira isolates in a hamster model. Vaccine, v. 26, n. 31, p. 3892-3896, 2008.0264-410Xhttps://www.arca.fiocruz.br/handle/icict/7532engNational Institute of HealthCharacterization of virulence of Leptospira isolates in a hamster model.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, BrasilUniversidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, BrasilUniversidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, BrasilUniversidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, BrasilFaculdade de Medicina Veterinária. Centro de Controle de Zoonoses. Pelotas, RS, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Weill Medical College of Cornell University. Division of International Medicine and Infectious Disease. New York, USAEffort has been made to identify protective antigens in order to develop a recombinant vaccine against leptospirosis. Several attempts failed to conclusively demonstrate efficacy of vaccine candidates due to the lack of an appropriate model of lethal leptospirosis. The purposes of our study were: (i) to test the virulence of leptospiral isolates from Brazil, which are representative of important serogroups that cause disease in humans and animals; and (ii) to standardize the lethal dose 50% (LD(50)) for each of the virulent strains using a hamster (Mesocricetus auratus) model. Five of seven Brazilian isolates induced lethality in a hamster model, with inocula lower than 200 leptospires. Histopathological examination of infected animals showed typical lesions found in both natural and experimental leptospirosis. Results described here demonstrated the potential use of Brazilian isolates as highly virulent strains in challenge experiments using hamster as an appropriate animal model for leptospirosis. Furthermore these strains may be useful in heterologous challenge studies which aim to evaluate cross-protective responses induced by sub-unit vaccine candidates.LeptospiraLeptospirosislethal doseisolation; animal model; virulenceAnimal modelVirulenceModelos Animais de DoençasLeptospira/patogenicidadeLeptospirose/microbiologiaAnimaisCricetinaeFemininoHumanosRim/patologiaDose Letal MedianaPulmão/patologiaMasculinoMesocricetusAnálise de SobrevidaVirulênciainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALSilva EF Characterization of virulence....pdfSilva EF Characterization of virulence....pdfapplication/pdf645232https://www.arca.fiocruz.br/bitstream/icict/7532/1/Silva%20EF%20Characterization%20of%20virulence....pdfa68e323e0a0815d562c6775db42456d8MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7532/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTSilva EF Characterization of virulence....pdf.txtSilva EF Characterization of virulence....pdf.txtExtracted texttext/plain34419https://www.arca.fiocruz.br/bitstream/icict/7532/5/Silva%20EF%20Characterization%20of%20virulence....pdf.txtd67d655d1002816cb3dc53dc7c981b4dMD55THUMBNAILSilva EF Characterization of virulence....pdf.jpgSilva EF Characterization of virulence....pdf.jpgGenerated Thumbnailimage/jpeg2323https://www.arca.fiocruz.br/bitstream/icict/7532/4/Silva%20EF%20Characterization%20of%20virulence....pdf.jpg1ed5224b95b795acf19aa0cb53f06371MD54icict/75322023-03-15 14:33:16.732oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:16Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Characterization of virulence of Leptospira isolates in a hamster model.
title Characterization of virulence of Leptospira isolates in a hamster model.
spellingShingle Characterization of virulence of Leptospira isolates in a hamster model.
Silva, Éverton Fagonde
Leptospira
Leptospirosis
lethal dose
isolation; animal model; virulence
Animal model
Virulence
Modelos Animais de Doenças
Leptospira/patogenicidade
Leptospirose/microbiologia
Animais
Cricetinae
Feminino
Humanos
Rim/patologia
Dose Letal Mediana
Pulmão/patologia
Masculino
Mesocricetus
Análise de Sobrevida
Virulência
title_short Characterization of virulence of Leptospira isolates in a hamster model.
title_full Characterization of virulence of Leptospira isolates in a hamster model.
title_fullStr Characterization of virulence of Leptospira isolates in a hamster model.
title_full_unstemmed Characterization of virulence of Leptospira isolates in a hamster model.
title_sort Characterization of virulence of Leptospira isolates in a hamster model.
author Silva, Éverton Fagonde
author_facet Silva, Éverton Fagonde
Santos, Cleiton Silva
Athanazio, Daniel Abensur
Seyffert, Núbia
Seixas, Fabiana K
Cerqueira, Gustavo Maia de
Fagundes, Michel Q
Brod, Claudiomar Soares
Reis, Mitermayer Galvão dos
Dellagostin, Odir Antonio
Ko, Albert Icksang
author_role author
author2 Santos, Cleiton Silva
Athanazio, Daniel Abensur
Seyffert, Núbia
Seixas, Fabiana K
Cerqueira, Gustavo Maia de
Fagundes, Michel Q
Brod, Claudiomar Soares
Reis, Mitermayer Galvão dos
Dellagostin, Odir Antonio
Ko, Albert Icksang
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Éverton Fagonde
Santos, Cleiton Silva
Athanazio, Daniel Abensur
Seyffert, Núbia
Seixas, Fabiana K
Cerqueira, Gustavo Maia de
Fagundes, Michel Q
Brod, Claudiomar Soares
Reis, Mitermayer Galvão dos
Dellagostin, Odir Antonio
Ko, Albert Icksang
dc.subject.en.pt_BR.fl_str_mv Leptospira
Leptospirosis
lethal dose
isolation; animal model; virulence
Animal model
Virulence
topic Leptospira
Leptospirosis
lethal dose
isolation; animal model; virulence
Animal model
Virulence
Modelos Animais de Doenças
Leptospira/patogenicidade
Leptospirose/microbiologia
Animais
Cricetinae
Feminino
Humanos
Rim/patologia
Dose Letal Mediana
Pulmão/patologia
Masculino
Mesocricetus
Análise de Sobrevida
Virulência
dc.subject.decs.pt_BR.fl_str_mv Modelos Animais de Doenças
Leptospira/patogenicidade
Leptospirose/microbiologia
Animais
Cricetinae
Feminino
Humanos
Rim/patologia
Dose Letal Mediana
Pulmão/patologia
Masculino
Mesocricetus
Análise de Sobrevida
Virulência
description Universidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2014-04-24T13:23:13Z
dc.date.available.fl_str_mv 2014-04-24T13:23:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, E. F. et al. Characterization of virulence of Leptospira isolates in a hamster model. Vaccine, v. 26, n. 31, p. 3892-3896, 2008.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7532
dc.identifier.issn.none.fl_str_mv 0264-410X
identifier_str_mv SILVA, E. F. et al. Characterization of virulence of Leptospira isolates in a hamster model. Vaccine, v. 26, n. 31, p. 3892-3896, 2008.
0264-410X
url https://www.arca.fiocruz.br/handle/icict/7532
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv National Institute of Health
publisher.none.fl_str_mv National Institute of Health
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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