Characterization of virulence of Leptospira isolates in a hamster model.
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2008 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/7532 |
Resumo: | Universidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil |
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Silva, Éverton FagondeSantos, Cleiton SilvaAthanazio, Daniel AbensurSeyffert, NúbiaSeixas, Fabiana KCerqueira, Gustavo Maia deFagundes, Michel QBrod, Claudiomar SoaresReis, Mitermayer Galvão dosDellagostin, Odir AntonioKo, Albert Icksang2014-04-24T13:23:13Z2014-04-24T13:23:13Z2008SILVA, E. F. et al. Characterization of virulence of Leptospira isolates in a hamster model. Vaccine, v. 26, n. 31, p. 3892-3896, 2008.0264-410Xhttps://www.arca.fiocruz.br/handle/icict/7532engNational Institute of HealthCharacterization of virulence of Leptospira isolates in a hamster model.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, BrasilUniversidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, BrasilUniversidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, BrasilUniversidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, BrasilFaculdade de Medicina Veterinária. Centro de Controle de Zoonoses. Pelotas, RS, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Weill Medical College of Cornell University. Division of International Medicine and Infectious Disease. New York, USAEffort has been made to identify protective antigens in order to develop a recombinant vaccine against leptospirosis. Several attempts failed to conclusively demonstrate efficacy of vaccine candidates due to the lack of an appropriate model of lethal leptospirosis. The purposes of our study were: (i) to test the virulence of leptospiral isolates from Brazil, which are representative of important serogroups that cause disease in humans and animals; and (ii) to standardize the lethal dose 50% (LD(50)) for each of the virulent strains using a hamster (Mesocricetus auratus) model. Five of seven Brazilian isolates induced lethality in a hamster model, with inocula lower than 200 leptospires. Histopathological examination of infected animals showed typical lesions found in both natural and experimental leptospirosis. Results described here demonstrated the potential use of Brazilian isolates as highly virulent strains in challenge experiments using hamster as an appropriate animal model for leptospirosis. Furthermore these strains may be useful in heterologous challenge studies which aim to evaluate cross-protective responses induced by sub-unit vaccine candidates.LeptospiraLeptospirosislethal doseisolation; animal model; virulenceAnimal modelVirulenceModelos Animais de DoençasLeptospira/patogenicidadeLeptospirose/microbiologiaAnimaisCricetinaeFemininoHumanosRim/patologiaDose Letal MedianaPulmão/patologiaMasculinoMesocricetusAnálise de SobrevidaVirulênciainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALSilva EF Characterization of virulence....pdfSilva EF Characterization of virulence....pdfapplication/pdf645232https://www.arca.fiocruz.br/bitstream/icict/7532/1/Silva%20EF%20Characterization%20of%20virulence....pdfa68e323e0a0815d562c6775db42456d8MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7532/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTSilva EF Characterization of virulence....pdf.txtSilva EF Characterization of virulence....pdf.txtExtracted texttext/plain34419https://www.arca.fiocruz.br/bitstream/icict/7532/5/Silva%20EF%20Characterization%20of%20virulence....pdf.txtd67d655d1002816cb3dc53dc7c981b4dMD55THUMBNAILSilva EF Characterization of virulence....pdf.jpgSilva EF Characterization of virulence....pdf.jpgGenerated Thumbnailimage/jpeg2323https://www.arca.fiocruz.br/bitstream/icict/7532/4/Silva%20EF%20Characterization%20of%20virulence....pdf.jpg1ed5224b95b795acf19aa0cb53f06371MD54icict/75322023-03-15 14:33:16.732oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:16Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Characterization of virulence of Leptospira isolates in a hamster model. |
| title |
Characterization of virulence of Leptospira isolates in a hamster model. |
| spellingShingle |
Characterization of virulence of Leptospira isolates in a hamster model. Silva, Éverton Fagonde Leptospira Leptospirosis lethal dose isolation; animal model; virulence Animal model Virulence Modelos Animais de Doenças Leptospira/patogenicidade Leptospirose/microbiologia Animais Cricetinae Feminino Humanos Rim/patologia Dose Letal Mediana Pulmão/patologia Masculino Mesocricetus Análise de Sobrevida Virulência |
| title_short |
Characterization of virulence of Leptospira isolates in a hamster model. |
| title_full |
Characterization of virulence of Leptospira isolates in a hamster model. |
| title_fullStr |
Characterization of virulence of Leptospira isolates in a hamster model. |
| title_full_unstemmed |
Characterization of virulence of Leptospira isolates in a hamster model. |
| title_sort |
Characterization of virulence of Leptospira isolates in a hamster model. |
| author |
Silva, Éverton Fagonde |
| author_facet |
Silva, Éverton Fagonde Santos, Cleiton Silva Athanazio, Daniel Abensur Seyffert, Núbia Seixas, Fabiana K Cerqueira, Gustavo Maia de Fagundes, Michel Q Brod, Claudiomar Soares Reis, Mitermayer Galvão dos Dellagostin, Odir Antonio Ko, Albert Icksang |
| author_role |
author |
| author2 |
Santos, Cleiton Silva Athanazio, Daniel Abensur Seyffert, Núbia Seixas, Fabiana K Cerqueira, Gustavo Maia de Fagundes, Michel Q Brod, Claudiomar Soares Reis, Mitermayer Galvão dos Dellagostin, Odir Antonio Ko, Albert Icksang |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Silva, Éverton Fagonde Santos, Cleiton Silva Athanazio, Daniel Abensur Seyffert, Núbia Seixas, Fabiana K Cerqueira, Gustavo Maia de Fagundes, Michel Q Brod, Claudiomar Soares Reis, Mitermayer Galvão dos Dellagostin, Odir Antonio Ko, Albert Icksang |
| dc.subject.en.pt_BR.fl_str_mv |
Leptospira Leptospirosis lethal dose isolation; animal model; virulence Animal model Virulence |
| topic |
Leptospira Leptospirosis lethal dose isolation; animal model; virulence Animal model Virulence Modelos Animais de Doenças Leptospira/patogenicidade Leptospirose/microbiologia Animais Cricetinae Feminino Humanos Rim/patologia Dose Letal Mediana Pulmão/patologia Masculino Mesocricetus Análise de Sobrevida Virulência |
| dc.subject.decs.pt_BR.fl_str_mv |
Modelos Animais de Doenças Leptospira/patogenicidade Leptospirose/microbiologia Animais Cricetinae Feminino Humanos Rim/patologia Dose Letal Mediana Pulmão/patologia Masculino Mesocricetus Análise de Sobrevida Virulência |
| description |
Universidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil |
| publishDate |
2008 |
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2008 |
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2014-04-24T13:23:13Z |
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2014-04-24T13:23:13Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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SILVA, E. F. et al. Characterization of virulence of Leptospira isolates in a hamster model. Vaccine, v. 26, n. 31, p. 3892-3896, 2008. |
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https://www.arca.fiocruz.br/handle/icict/7532 |
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0264-410X |
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SILVA, E. F. et al. Characterization of virulence of Leptospira isolates in a hamster model. Vaccine, v. 26, n. 31, p. 3892-3896, 2008. 0264-410X |
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National Institute of Health |
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National Institute of Health |
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