A heart-specific CD4+ T-cell line obtained from a chronic chagasic mouse induces carditis in heart-immunized mice and rejection of normal heart transplants in the absence of Trypanosoma cruzi.

Santos, Ricardo Ribeiro dos; Mengele Junior, José Orivaldo; Postol, Edilberto; Soares, R. A. O; Fernandez, E. Ferreira; Soares, Milena Botelho Pereira; Pontes-de-Carvalho, Lain Carlos

A heart-specific CD4+ T-cell line obtained from a chronic chagasic mouse induces carditis in heart-immunized mice and rejection of normal heart transplants in the absence of Trypanosoma cruzi.

Bibliographic Details
Main Author:	Santos, Ricardo Ribeiro dos
Publication Date:	2001
Other Authors:	Mengele Junior, José Orivaldo, Postol, Edilberto, Soares, R. A. O, Fernandez, E. Ferreira, Soares, Milena Botelho Pereira, Pontes-de-Carvalho, Lain Carlos
Format:	Article
Language:	eng
Source:	Repositório Institucional da FIOCRUZ (ARCA)
Download full:	https://www.arca.fiocruz.br/handle/icict/5625
Summary:	Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil

Item metadata

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network_acronym_str	CRUZ
network_name_str	Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str	2135
spelling	Santos, Ricardo Ribeiro dosMengele Junior, José OrivaldoPostol, EdilbertoSoares, R. A. OFernandez, E. FerreiraSoares, Milena Botelho PereiraPontes-de-Carvalho, Lain Carlos2012-10-02T20:59:07Z2012-10-02T20:59:07Z2001SANTOS, R. R. dos et al. A heart-specific CD4+ T-cell line obtained from a chronic chagasic mouse induces carditis in heart-immunized mice and rejection of normal heart transplants in the absence of Trypanosoma cruzi. Parasite Immunology, v. 23, n. 2, p. 93-101, feb. 2001.0141-9838https://www.arca.fiocruz.br/handle/icict/5625engA heart-specific CD4+ T-cell line obtained from a chronic chagasic mouse induces carditis in heart-immunized mice and rejection of normal heart transplants in the absence of Trypanosoma cruzi.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Imunologia. São Paulo, SP, BrasilFundação Oswaldo Cruz. Farmanguinhos. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Farmanguinhos. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilTo study the role of autoreactive T cells in the pathogenesis of cardiomyopathy in Chagas' disease, we generated a cell line by repeated in vitro antigenic stimulation of purified splenic CD4+ T lymphocytes from a chronically Trypanosoma cruzi-infected mouse. Cells from this line were confirmed to be CD4+ CD8- and proliferated upon stimulation with soluble heart antigens from different animal species, as well as with T. cruzi antigen, in the presence of syngeneic feeder cells. In vitro antigen stimulation of the cell line produced a Th1 cytokine profile, with high levels of IFNgamma and IL-2 and absence of IL-4, IL-5 and IL-10. The cell line also terminated the beating of fetal heart clusters in vitro when cocultured with irradiated syngeneic normal spleen cells. In situ injection of the cell line into well established heart transplants also induced the cessation of heart beating. Finally, adoptive transfer of the cell line to heart-immunized or T. cruzi-infected BALB/c nude mice caused intense heart inflammation.T-cell lineTrypanosoma cruziAutoreactivityCarditisChagas' diseaseLinfócitos T CD4-Positivos/imunologiaDoença de Chagas/imunologiaTrypanosoma cruziAnimaisLinhagem CelularDoença CrônicaTécnicas de CoculturaModelos Animais de DoençasRejeição de EnxertoTransplante de CoraçãoInterferon gama/análiseInterleucina-2/análiseCamundongosCamundongos Endogâmicos BALB CCamundongos Endogâmicos C57BLCamundongos Endogâmicos DBACamundongos NusMiocardite/imunologiaMiocárdio/citologiaMiocárdio/imunologiaCoelhosRatosBaço/imunologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALRibeiro-Dos_Santos R A heart specific CD4+ T-cell....pdfRibeiro-Dos_Santos R A heart specific CD4+ T-cell....pdfapplication/pdf365996https://www.arca.fiocruz.br/bitstream/icict/5625/1/Ribeiro-Dos_Santos%20R%20A%20heart%20specific%20CD4%2b%20T-cell....pdf63005200e720e052c9d7923012979ecaMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82008https://www.arca.fiocruz.br/bitstream/icict/5625/2/license.txt06db3f0df647fd9ddff657403bbe0ee2MD52TEXTRibeiro-Dos_Santos R A heart specific CD4+ T-cell....pdf.txtRibeiro-Dos_Santos R A heart specific CD4+ T-cell....pdf.txtExtracted texttext/plain34457https://www.arca.fiocruz.br/bitstream/icict/5625/5/Ribeiro-Dos_Santos%20R%20A%20heart%20specific%20CD4%2b%20T-cell....pdf.txtbed27b2b20c21f2323d871ecfcc5ab4fMD55THUMBNAILRibeiro-Dos_Santos R A heart specific CD4+ T-cell....pdf.jpgRibeiro-Dos_Santos R A heart specific CD4+ T-cell....pdf.jpgGenerated Thumbnailimage/jpeg2059https://www.arca.fiocruz.br/bitstream/icict/5625/4/Ribeiro-Dos_Santos%20R%20A%20heart%20specific%20CD4%2b%20T-cell....pdf.jpgacc1ad708572342568b64ee9d878ae34MD54icict/56252023-03-15 14:33:12.689oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:12Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv	A heart-specific CD4+ T-cell line obtained from a chronic chagasic mouse induces carditis in heart-immunized mice and rejection of normal heart transplants in the absence of Trypanosoma cruzi.
title	A heart-specific CD4+ T-cell line obtained from a chronic chagasic mouse induces carditis in heart-immunized mice and rejection of normal heart transplants in the absence of Trypanosoma cruzi.
spellingShingle	A heart-specific CD4+ T-cell line obtained from a chronic chagasic mouse induces carditis in heart-immunized mice and rejection of normal heart transplants in the absence of Trypanosoma cruzi. Santos, Ricardo Ribeiro dos T-cell line Trypanosoma cruzi Autoreactivity Carditis Chagas' disease Linfócitos T CD4-Positivos/imunologia Doença de Chagas/imunologia Trypanosoma cruzi Animais Linhagem Celular Doença Crônica Técnicas de Cocultura Modelos Animais de Doenças Rejeição de Enxerto Transplante de Coração Interferon gama/análise Interleucina-2/análise Camundongos Camundongos Endogâmicos BALB C Camundongos Endogâmicos C57BL Camundongos Endogâmicos DBA Camundongos Nus Miocardite/imunologia Miocárdio/citologia Miocárdio/imunologia Coelhos Ratos Baço/imunologia
title_short	A heart-specific CD4+ T-cell line obtained from a chronic chagasic mouse induces carditis in heart-immunized mice and rejection of normal heart transplants in the absence of Trypanosoma cruzi.
title_full	A heart-specific CD4+ T-cell line obtained from a chronic chagasic mouse induces carditis in heart-immunized mice and rejection of normal heart transplants in the absence of Trypanosoma cruzi.
title_fullStr	A heart-specific CD4+ T-cell line obtained from a chronic chagasic mouse induces carditis in heart-immunized mice and rejection of normal heart transplants in the absence of Trypanosoma cruzi.
title_full_unstemmed	A heart-specific CD4+ T-cell line obtained from a chronic chagasic mouse induces carditis in heart-immunized mice and rejection of normal heart transplants in the absence of Trypanosoma cruzi.
title_sort	A heart-specific CD4+ T-cell line obtained from a chronic chagasic mouse induces carditis in heart-immunized mice and rejection of normal heart transplants in the absence of Trypanosoma cruzi.
author	Santos, Ricardo Ribeiro dos
author_facet	Santos, Ricardo Ribeiro dos Mengele Junior, José Orivaldo Postol, Edilberto Soares, R. A. O Fernandez, E. Ferreira Soares, Milena Botelho Pereira Pontes-de-Carvalho, Lain Carlos
author_role	author
author2	Mengele Junior, José Orivaldo Postol, Edilberto Soares, R. A. O Fernandez, E. Ferreira Soares, Milena Botelho Pereira Pontes-de-Carvalho, Lain Carlos
author2_role	author author author author author author
dc.contributor.author.fl_str_mv	Santos, Ricardo Ribeiro dos Mengele Junior, José Orivaldo Postol, Edilberto Soares, R. A. O Fernandez, E. Ferreira Soares, Milena Botelho Pereira Pontes-de-Carvalho, Lain Carlos
dc.subject.en.pt_BR.fl_str_mv	T-cell line Trypanosoma cruzi Autoreactivity Carditis Chagas' disease
topic	T-cell line Trypanosoma cruzi Autoreactivity Carditis Chagas' disease Linfócitos T CD4-Positivos/imunologia Doença de Chagas/imunologia Trypanosoma cruzi Animais Linhagem Celular Doença Crônica Técnicas de Cocultura Modelos Animais de Doenças Rejeição de Enxerto Transplante de Coração Interferon gama/análise Interleucina-2/análise Camundongos Camundongos Endogâmicos BALB C Camundongos Endogâmicos C57BL Camundongos Endogâmicos DBA Camundongos Nus Miocardite/imunologia Miocárdio/citologia Miocárdio/imunologia Coelhos Ratos Baço/imunologia
dc.subject.decs.pt_BR.fl_str_mv	Linfócitos T CD4-Positivos/imunologia Doença de Chagas/imunologia Trypanosoma cruzi Animais Linhagem Celular Doença Crônica Técnicas de Cocultura Modelos Animais de Doenças Rejeição de Enxerto Transplante de Coração Interferon gama/análise Interleucina-2/análise Camundongos Camundongos Endogâmicos BALB C Camundongos Endogâmicos C57BL Camundongos Endogâmicos DBA Camundongos Nus Miocardite/imunologia Miocárdio/citologia Miocárdio/imunologia Coelhos Ratos Baço/imunologia
description	Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
publishDate	2001
dc.date.issued.fl_str_mv	2001
dc.date.accessioned.fl_str_mv	2012-10-02T20:59:07Z
dc.date.available.fl_str_mv	2012-10-02T20:59:07Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	SANTOS, R. R. dos et al. A heart-specific CD4+ T-cell line obtained from a chronic chagasic mouse induces carditis in heart-immunized mice and rejection of normal heart transplants in the absence of Trypanosoma cruzi. Parasite Immunology, v. 23, n. 2, p. 93-101, feb. 2001.
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/5625
dc.identifier.issn.none.fl_str_mv	0141-9838
identifier_str_mv	SANTOS, R. R. dos et al. A heart-specific CD4+ T-cell line obtained from a chronic chagasic mouse induces carditis in heart-immunized mice and rejection of normal heart transplants in the absence of Trypanosoma cruzi. Parasite Immunology, v. 23, n. 2, p. 93-101, feb. 2001. 0141-9838
url	https://www.arca.fiocruz.br/handle/icict/5625
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
instname_str	Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str	FIOCRUZ
institution	FIOCRUZ
reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
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A heart-specific CD4+ T-cell line obtained from a chronic chagasic mouse induces carditis in heart-immunized mice and rejection of normal heart transplants in the absence of Trypanosoma cruzi.

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