An alternative technique to reveal polysaccharides in Toxoplasma gondii tissue cysts

Detalhes bibliográficos
Autor(a) principal: Guimarães, Erick Vaz
Data de Publicação: 2003
Outros Autores: Carvalho, Laís de, Barbosa, Helene Santos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/34338
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-estrutura e Biologia Celular. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ. Brasil.
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spelling Guimarães, Erick VazCarvalho, Laís deBarbosa, Helene Santos2019-07-23T12:56:09Z2019-07-23T12:56:09Z2003GUIMARÃES, Erick Vaz; CARVALHO, Laís de; BARBOSA, Helene Santos. An alternative technique to reveal polysaccharides in Toxoplasma gondii tissue cysts. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 98, n. 7, p. 915-917, Oct. 2003.0074-0276https://www.arca.fiocruz.br/handle/icict/3433810.1590/s0074-027620030007000101678-8060engFundação Oswaldo Cruz. Instituto Oswaldo CruzToxoplasma gondiiCitoquímica ultraestruturalGrânulos de amilopectinaCistos teciduaisToxoplasma gondiiAmylopectin granulesTissue cystsUltrastructural cytochemistryAn alternative technique to reveal polysaccharides in Toxoplasma gondii tissue cystsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-estrutura e Biologia Celular. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ. Brasil.Universidade do Estado do Rio de Janeiro. Departamento de Histologia e Embriologia. Laboratório de Cultura de Células. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-estrutura e Biologia Celular. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ. Brasil.Ultrathin sections of tissue cysts isolated from the brain of Toxoplasma gondii infected mice were submitted to two different methodologies derived from the periodic acid - Schiff's reagent (PAS) technique. The use of osmium tetroxide vapor as a developing agent of the aldehyde oxidation to reveal polysaccharides with periodic acid resulted in positive reaction in amylopectin granules in bradyzoites, as well as in the wall and matrix of the cysts, with excellent increment of the ultrastructural morphology. This technique can be used for study of T. gondii-host cell intracellular cycle, the differentiation tachyzoite-bradyzoite, and also for the formation of cysts into the host cells.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/34338/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALHeleneBarbosa_ErickGuimaraes_etal_IOC_2003.pdfHeleneBarbosa_ErickGuimaraes_etal_IOC_2003.pdfapplication/pdf996353https://www.arca.fiocruz.br/bitstream/icict/34338/2/HeleneBarbosa_ErickGuimaraes_etal_IOC_2003.pdf9ea71828541a92b9e210fb244dbd3572MD52TEXTHeleneBarbosa_ErickGuimaraes_etal_IOC_2003.pdf.txtHeleneBarbosa_ErickGuimaraes_etal_IOC_2003.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv An alternative technique to reveal polysaccharides in Toxoplasma gondii tissue cysts
title An alternative technique to reveal polysaccharides in Toxoplasma gondii tissue cysts
spellingShingle An alternative technique to reveal polysaccharides in Toxoplasma gondii tissue cysts
Guimarães, Erick Vaz
Toxoplasma gondii
Citoquímica ultraestrutural
Grânulos de amilopectina
Cistos teciduais
Toxoplasma gondii
Amylopectin granules
Tissue cysts
Ultrastructural cytochemistry
title_short An alternative technique to reveal polysaccharides in Toxoplasma gondii tissue cysts
title_full An alternative technique to reveal polysaccharides in Toxoplasma gondii tissue cysts
title_fullStr An alternative technique to reveal polysaccharides in Toxoplasma gondii tissue cysts
title_full_unstemmed An alternative technique to reveal polysaccharides in Toxoplasma gondii tissue cysts
title_sort An alternative technique to reveal polysaccharides in Toxoplasma gondii tissue cysts
author Guimarães, Erick Vaz
author_facet Guimarães, Erick Vaz
Carvalho, Laís de
Barbosa, Helene Santos
author_role author
author2 Carvalho, Laís de
Barbosa, Helene Santos
author2_role author
author
dc.contributor.author.fl_str_mv Guimarães, Erick Vaz
Carvalho, Laís de
Barbosa, Helene Santos
dc.subject.other.pt_BR.fl_str_mv Toxoplasma gondii
Citoquímica ultraestrutural
Grânulos de amilopectina
Cistos teciduais
topic Toxoplasma gondii
Citoquímica ultraestrutural
Grânulos de amilopectina
Cistos teciduais
Toxoplasma gondii
Amylopectin granules
Tissue cysts
Ultrastructural cytochemistry
dc.subject.en.pt_BR.fl_str_mv Toxoplasma gondii
Amylopectin granules
Tissue cysts
Ultrastructural cytochemistry
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-estrutura e Biologia Celular. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ. Brasil.
publishDate 2003
dc.date.issued.fl_str_mv 2003
dc.date.accessioned.fl_str_mv 2019-07-23T12:56:09Z
dc.date.available.fl_str_mv 2019-07-23T12:56:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GUIMARÃES, Erick Vaz; CARVALHO, Laís de; BARBOSA, Helene Santos. An alternative technique to reveal polysaccharides in Toxoplasma gondii tissue cysts. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 98, n. 7, p. 915-917, Oct. 2003.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/34338
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/s0074-02762003000700010
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv GUIMARÃES, Erick Vaz; CARVALHO, Laís de; BARBOSA, Helene Santos. An alternative technique to reveal polysaccharides in Toxoplasma gondii tissue cysts. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 98, n. 7, p. 915-917, Oct. 2003.
0074-0276
10.1590/s0074-02762003000700010
1678-8060
url https://www.arca.fiocruz.br/handle/icict/34338
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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